Optimizing in vitro shoot propagation of Indonesian true seeds of shallot (TSS) using MS and DKW media with BAP: English

Using shallot botanical seeds or true seed of shallot (TSS) as planting material can produce plants free from bulb-borne disease and be more technically and economically efficient. However, its application in the field still has limitations. The objective of this study was to evaluate the in vitro propagation media containing a combination of MS and DKW media with the addition of BAP  on the growth parameters and multiplication frequency as well as plantlets condition of three cultivars of TSS to initiation support the supply of quality seeds in the field.  The seeds were germinated in vitro using an MS medium, and then aseptic TSS seedlings were arranged using a completely randomized design with two factors and twelve replications. The first factor was media composition.  MS (Murashige and Skoog) and DKW (Driver Kuniyaki Walnut) media were used for the in vitro shoot propagation media in combination with BAP (Benzyl Amino Purine) at the level of concentrations:  0, 1, 2 and 4 mgL-1.   The second factor was TSS cultivars: Bima Brebes, Trisula and Tuktuk.  The result showed that Bima Brebes and Trisula cultivars significantly differed in multiplication frequency from the Tuktuk cultivar. DKW medium with 4 mgL-1 BAP produced the highest multiplication frequency but not significantly different with 4 mgL-1 BAP in MS and 2 mgL-1 BAP in DKW media. Adding 1-2 mgL-1 BAP in the DKW medium could induce new shoots on the Bima Brebes cultivar with better performance, though with lower multiplication frequency. Using shallot botanical seeds or true seed of shallot (TSS) as planting material can produce plants free from bulb-borne disease and be more technically and economically efficient. However, its application in the field still has limitations. The objective of this study was to evaluate the in vitro propagation media containing a combination of MS and DKW media with the addition of BAP  on the growth parameters and multiplication frequency as well as plantlets condition of three cultivars of TSS to initiation support the supply of quality seeds in the field.  The seeds were germinated in vitro using an MS medium, and then aseptic TSS seedlings were arranged using a completely randomized design with two factors and twelve replications. The first factor was media composition.  MS (Murashige and Skoog) and DKW (Driver Kuniyaki Walnut) media were used for the in vitro shoot propagation media in combination with BAP (Benzyl Amino Purine) at the level of concentrations:  0, 1, 2 and 4 mgL-1.   The second factor was TSS cultivars: Bima Brebes, Trisula and Tuktuk.  The result showed that Bima Brebes and Trisula cultivars significantly differed in multiplication frequency from the Tuktuk cultivar. DKW medium with 4 mgL-1 BAP produced the highest multiplication frequency but not significantly different with 4 mgL-1 BAP in MS and 2 mgL-1 BAP in DKW media. Adding 1-2 mgL-1 BAP in the DKW medium could induce new shoots on the Bima Brebes cultivar with better performance, though with lower multiplication frequency.

Molecular Detection of the Mutations in Khoderi Olive cultivar : NO

The characterization of olive cultivars using molecular markers is necessary for genetic diversity conservation, cultivar verifiction, and the identification of locally adapted genotypes. This study searches for genetic variation in the Khoderi olive cultivar from Karbala, Iraq, utilization two genetic molecular regions: the nuclear ribosomal ITS1 spacer and the plastid matK gene. Through DNA was extracted using a modified CTAB protocol, amplified by PCR, and get sequenced utilization Sanger sequencing. Alignment and comparative analysis with reference sequences deposited in Gen-Bank the results revealed 92.3% similarity of the ITS1 sequence with the Khoderi-Bakraju cultivar with serial number (OQ134700.1), indicating genetic affinity but insufficient similarity for confirmed cultivar identity. The matK region showed 96% similarity with an Olea europaea subsp. europaea plastid genome with serial number (MG372119.1), we notice reflecting high conservation of the chloroplast coding sequences. Multiple SNPs and small indels were detected in both regions, representing intraspecific polymorphisms. The combined data suggest that the studied sample belongs to the broader O. europaea gene pool but may represent a distinct local genotype. Further analyses using high-resolution molecular markers (SSR, SNP-based GBS) are recommended to refine cultivar differentiation.The characterization of olive cultivars using molecular markers is necessary for genetic diversity conservation, cultivar verifiction, and the identification of locally adapted genotypes. This study searches for genetic variation in the Khoderi olive cultivar from Karbala, Iraq, utilization two genetic molecular regions: the nuclear ribosomal ITS1 spacer and the plastid matK gene. Through DNA was extracted using a modified CTAB protocol, amplified by PCR, and get sequenced utilization Sanger sequencing. Alignment and comparative analysis with reference sequences deposited in Gen-Bank the results revealed 92.3% similarity of the ITS1 sequence with the Khoderi-Bakraju cultivar with serial number (OQ134700.1), indicating genetic affinity but insufficient similarity for confirmed cultivar identity. The matK region showed 96% similarity with an Olea europaea subsp. europaea plastid genome with serial number (MG372119.1), we notice reflecting high conservation of the chloroplast coding sequences. Multiple SNPs and small indels were detected in both regions, representing intraspecific polymorphisms. The combined data suggest that the studied sample belongs to the broader O. europaea gene pool but may represent a distinct local genotype. Further analyses using high-resolution molecular markers (SSR, SNP-based GBS) are recommended to refine cultivar differentiation

Dominance and complexity of Begomovirus Species infection on eggplant in lowlands and highlands

Eggplant (Solanum melongena L.) is an important Indonesian crop increasingly threatened by begomoviruses that reduce yield and quality. Transmitted persistently by the whitefly Bemisia tabaci, these viruses are genetically diverse and often occur in mixed infections, intensifying disease severity and complicating control. This study aimed to identify begomovirus species infecting eggplant, determine their geographic distribution and dominance, and characterize the associated B. tabaci biotypes across major eggplant-producing regions of Java, Indonesia. Field surveys were conducted in five provinces—Banten, West Java, Central Java, Yogyakarta, and East Java—covering 30 lowland and 15 highland sites. From each site, 20 symptomatic plants were collected for molecular analysis, while disease incidence (DI) and severity (DS) were assessed from 200 plants. Across 45 sites, mean DI and DS reached 54.0% and 32.7% in lowlands and 34.8% and 26.8% in highlands, respectively. PCR amplification and sequencing confirmed three begomovirus species: Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), Pepper yellow leaf curl Indonesia virus (PepYLCIV), and Tomato leaf curl New Delhi virus (ToLCNDV). TYLCKaV was the most prevalent, occurring alone or in combination with PepYLCIV, particularly in lowlands, while ToLCNDV occurred at low frequencies, mainly in mixed infections. Four B. tabaci biotypes (A, B, Q, and AN) were detected, with biotype A dominating in lowlands and Q prevailing in highlands. This study provides the first island-wide molecular mapping of begomovirus species and B. tabaci biotypes infecting eggplant in Indonesia and elucidates the complex virus–vector interactions that shape eggplant disease epidemiology in tropical Asia.Eggplant (Solanum melongena L.) is an important Indonesian crop increasingly threatened by begomoviruses that reduce yield and quality. Transmitted persistently by the whitefly Bemisia tabaci, these viruses are genetically diverse and often occur in mixed infections, intensifying disease severity and complicating control. This study aimed to identify begomovirus species infecting eggplant, determine their geographic distribution and dominance, and characterize the associated B. tabaci biotypes across major eggplant-producing regions of Java, Indonesia. Field surveys were conducted in five provinces—Banten, West Java, Central Java, Yogyakarta, and East Java—covering 30 lowland and 15 highland sites. From each site, 20 symptomatic plants were collected for molecular analysis, while disease incidence (DI) and severity (DS) were assessed from 200 plants. Across 45 sites, mean DI and DS reached 54.0% and 32.7% in lowlands and 34.8% and 26.8% in highlands, respectively. PCR amplification and sequencing confirmed three begomovirus species: Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), Pepper yellow leaf curl Indonesia virus (PepYLCIV), and Tomato leaf curl New Delhi virus (ToLCNDV). TYLCKaV was the most prevalent, occurring alone or in combination with PepYLCIV, particularly in lowlands, while ToLCNDV occurred at low frequencies, mainly in mixed infections. Four B. tabaci biotypes (A, B, Q, and AN) were detected, with biotype A dominating in lowlands and Q prevailing in highlands. This study provides the first island-wide molecular mapping of begomovirus species and B. tabaci biotypes infecting eggplant in Indonesia and elucidates the complex virus–vector interactions that shape eggplant disease epidemiology in tropical Asia

Resilience and sustainability in the production of Ullucus tuberosus under a biocultural approach in Andean communities

This article evaluates the agroecological and biocultural impact of Ullucus tuberosus cultivation in the Montúfar canton of Carchi province, Ecuador. Focusing on the "Producampo" Association, made up mostly of women, the research analyzes how they combine traditional and sustainable agricultural practices to ensure productive resilience and preserve biodiversity. Through qualitative methodologies such as interviews and participant observation, it was shown that 87.5% of farmers are women aged 39 to 75 who work more than 56 hours per week on their small plots, most of which are less than one hectare. Agroecological practices include the use of organic fertilizers, crop rotation, and natural methods for pest control. These actions not only minimize the use of chemical inputs but also strengthen sustainability and food security. Furthermore, bioculturalism plays a key role: farmers apply ancestral knowledge such as the lunar calendar and seed exchange, promoting social cohesion and adaptation to climate challenges. Despite limitations such as limited access to land, Ullucus tuberosus remains essential to the local economy and food supply. The study concludes by highlighting the need for public policies that support the integration of traditional knowledge with scientific innovations to strengthen agricultural sustainability and the empowerment of rural communities, especially women, within an inclusive development model.This article evaluates the agroecological and biocultural impact of Ullucus tuberosus cultivation in the Montúfar canton of Carchi province, Ecuador. Focusing on the "Producampo" Association, made up mostly of women, the research analyzes how they combine traditional and sustainable agricultural practices to ensure productive resilience and preserve biodiversity. Through qualitative methodologies such as interviews and participant observation, it was shown that 87.5% of farmers are women aged 39 to 75 who work more than 56 hours per week on their small plots, most of which are less than one hectare. Agroecological practices include the use of organic fertilizers, crop rotation, and natural methods for pest control. These actions not only minimize the use of chemical inputs but also strengthen sustainability and food security. Furthermore, bioculturalism plays a key role: farmers apply ancestral knowledge such as the lunar calendar and seed exchange, promoting social cohesion and adaptation to climate challenges. Despite limitations such as limited access to land, Ullucus tuberosus remains essential to the local economy and food supply. The study concludes by highlighting the need for public policies that support the integration of traditional knowledge with scientific innovations to strengthen agricultural sustainability and the empowerment of rural communities, especially women, within an inclusive development model

Overview and challenges of citrus industry in Sergipe from 2010 to 2022

The Brazilian citrus industry is one of the country\u27s most significant economic and social sectors, positioning Brazil as a global leader in this field. In the state of Sergipe, the citrus industry represents 3% of the Gross Domestic Product (GDP), playing a crucial role in the local economy, with citrus being one of the region\u27s key agricultural products. Given this context, the objective of this study was to analyze the opportunities and challenges faced by Sergipe\u27s citrus sector between 2010 and 2022. The analysis focused on production and commercialization data for sweet oranges, considering climatic, economic, and competitive factors that impact the sector. Data on planted areas, production, crop yield, prices, and rainfall indices were gathered from public research platforms, including the Brazilian Institute of Geography and Statistics (IBGE) and the Agricultural Development Company of Sergipe (EMDAGRO). Throughout the evaluated historical series, there were fluctuations in orange planted areas, with a reduction of 45%, and a 52% decline in fruit production, largely due to climatic conditions and phytosanitary issues. Despite these ongoing challenges, projections suggest a 0.5% increase in orange yield in the coming years. High international demand and changing consumption patterns for both the fruit and its juice, as seen during the COVID-19 pandemic, present an opportunity for local producers, provided that proper crop management practices are implemented to address the existing challenges.The Brazilian citrus industry is one of the country\u27s most significant economic and social sectors, positioning Brazil as a global leader in this field. In the state of Sergipe, the citrus industry represents 3% of the Gross Domestic Product (GDP), playing a crucial role in the local economy, with citrus being one of the region\u27s key agricultural products. Given this context, the objective of this study was to analyze the opportunities and challenges faced by Sergipe\u27s citrus sector between 2010 and 2022. The analysis focused on production and commercialization data for sweet oranges, considering climatic, economic, and competitive factors that impact the sector. Data on planted areas, production, crop yield, prices, and rainfall indices were gathered from public research platforms, including the Brazilian Institute of Geography and Statistics (IBGE) and the Agricultural Development Company of Sergipe (EMDAGRO). Throughout the evaluated historical series, there were fluctuations in orange planted areas, with a reduction of 45%, and a 52% decline in fruit production, largely due to climatic conditions and phytosanitary issues. Despite these ongoing challenges, projections suggest a 0.5% increase in orange yield in the coming years. High international demand and changing consumption patterns for both the fruit and its juice, as seen during the COVID-19 pandemic, present an opportunity for local producers, provided that proper crop management practices are implemented to address the existing challenges

Experimental plan for radish culture: Experimental plan

One of the main stages of agricultural testing is experimental planning. This study aimed to propose an experimental plan, with estimates of plot size, sample size and number of repetitions in the radish crop. The uniformity sowing tests were carried out in three plots of 6.5 m in length, arranged in four rows with spacing of 0.20 m between them and 0.10 m between plants, totaling 64 plants per row. The cultivars used were Redondo Vermelho Gigante and Gigante Siculo. Each plant was considered a basic experimental unit, and the number of leaves, shoot length, root circumference, plant height, root mass and shoot mass were evaluated. For each line, the plot size was estimated by the method of maximum curvature of the coefficient of variation. Sample size estimation followed Cochran\u27s methodology. To estimate the number of repetitions, the least significant difference from the Tukey test was used. It is recommended to use the largest plot size (13 plants) and sample size with twelve plants in the plot. For a minimum difference of 50% to be considered by Tukey\u27s test, between six and twelve repetitions are necessary.One of the main stages of agricultural testing is experimental planning. This study aimed to propose an experimental plan, with estimates of plot size, sample size and number of repetitions in the radish crop. The uniformity sowing tests were carried out in three plots of 6.5 m in length, arranged in four rows with spacing of 0.20 m between them and 0.10 m between plants, totaling 64 plants per row. The cultivars used were Redondo Vermelho Gigante and Gigante Siculo. Each plant was considered a basic experimental unit, and the number of leaves, shoot length, root circumference, plant height, root mass and shoot mass were evaluated. For each line, the plot size was estimated by the method of maximum curvature of the coefficient of variation. Sample size estimation followed Cochran\u27s methodology. To estimate the number of repetitions, the least significant difference from the Tukey test was used. It is recommended to use the largest plot size (13 plants) and sample size with twelve plants in the plot. For a minimum difference of 50% to be considered by Tukey\u27s test, between six and twelve repetitions are necessary

Fertilizing and protective potential of castor oil cake on soil and morphological parameters of eggplant (Solanuma melongena)

Vegetable crops are the backbone of agriculture. However, low soil fertility, pest spreading, high cost of chemical fertilizers and effects of pesticides have prompted the search for sustainable alternatives. This study investigated the action of castor oil cake doses on soil properties, rooty nematodes and eggplant parameters. The experiment was conducted on a Fisher block design (4x5). The treatments applied included the negative control, Triple 15 and different doses of castor oil cake (1; 1.9 and 2.8 t/ha). The incorporation of the oil cake changed the texture from clayey-sandy to loamy-sandy. The soil pH changed from 3.9 to 4.6. The saturation rate, the sum of exchangeable bases and the cation exchange capacity also increased by 30 to 41% respectively; 0.94 to 1.36% and 3.1 to 3.29%. Nitrogen content increased from 0.048 to 0.084%, phosphorus and potassium contents did not change. Organic matter rate increased from 0.84 to 2.92%. Analysis of variance showed a significant effect (p < 0.05). Plant mortality rates due to nematode were 25.25% for the negative control, 19.12% for the positive control (triple 15), 13% for D1 (1 t/ha of castor cake) and 0.00% for D2 (1.9 t/ha of castor cake) and D3 (2.8 t/ha of castor cake). Yields varied (from 3.58 to 6.80 t/ha) depending on the treatments.Vegetable crops are the backbone of agriculture. However, low soil fertility, pest spreading, high cost of chemical fertilizers and effects of pesticides have prompted the search for sustainable alternatives. This study investigated the action of castor oil cake doses on soil properties, rooty nematodes and eggplant parameters. The experiment was conducted on a Fisher block design (4x5). The treatments applied included the negative control, Triple 15 and different doses of castor oil cake (1; 1.9 and 2.8 t/ha). The incorporation of the oil cake changed the texture from clayey-sandy to loamy-sandy. The soil pH changed from 3.9 to 4.6. The saturation rate, the sum of exchangeable bases and the cation exchange capacity also increased by 30 to 41% respectively; 0.94 to 1.36% and 3.1 to 3.29%. Nitrogen content increased from 0.048 to 0.084%, phosphorus and potassium contents did not change. Organic matter rate increased from 0.84 to 2.92%. Analysis of variance showed a significant effect (p < 0.05). Plant mortality rates due to nematode were 25.25% for the negative control, 19.12% for the positive control (triple 15), 13% for D1 (1 t/ha of castor cake) and 0.00% for D2 (1.9 t/ha of castor cake) and D3 (2.8 t/ha of castor cake). Yields varied (from 3.58 to 6.80 t/ha) depending on the treatments

Chemical and physicochemical profiles of essential oil from Piper betel (L.) cultivated in different regions of Vietnam : Chemical and physicochemical profiles of essential oil from Piper betel (L.) cultivated in different regions of Vietnam

This study aimed to investigate the variation of essential oils from Piper betel cultivated in three different geographic regions of Vietnam (Hoc Mon, Tien Giang, and Dak Lak). The extraction of essential oil was conducted by hydro-distillation for 3 h using a Clevenger-type apparatus. GC-MS was employed for the analysis of obtained essential oil. Physicochemical parameters were determined as per standard test methods. GC-MS revealed the highest amount of chavibetol present in all three essential oils of Piper betel cultivated in three different geographic regions of Vietnam: Hoc Mon (49.077%), Tien Giang (39.982%) and Dak Lak (34.888%). In contrast to the absence of Cadinene in both Hoc Mon and Tien Giang essential oils, there was remarkably an extremely high content of Cadinene present in Dak Lak essential oil, accounting for approximately 49% of the total constituents of essential oil. α-Cadinene was recorded to be the highest quantity (19.826%), followed by γ-Cadinene (15.470%) and δ-Cadinene (13.369%). Nature variation in different geographic regions of Vietnam (Hoc Mon, Tien Giang, and Dak Lak) plays a key role in the diversification of essential oil composition in Piper betel.This study aimed to investigate the variation of essential oils from Piper betel cultivated in three different geographic regions of Vietnam (Hoc Mon, Tien Giang, and Dak Lak). The extraction of essential oil was conducted by hydro-distillation for 3 h using a Clevenger-type apparatus. GC-MS was employed for the analysis of obtained essential oil. Physicochemical parameters were determined as per standard test methods. GC-MS revealed the highest amount of chavibetol present in all three essential oils of Piper betel cultivated in three different geographic regions of Vietnam: Hoc Mon (49.077%), Tien Giang (39.982%) and Dak Lak (34.888%). In contrast to the absence of Cadinene in both Hoc Mon and Tien Giang essential oils, there was remarkably an extremely high content of Cadinene present in Dak Lak essential oil, accounting for approximately 49% of the total constituents of essential oil. α-Cadinene was recorded to be the highest quantity (19.826%), followed by γ-Cadinene (15.470%) and δ-Cadinene (13.369%). Nature variation in different geographic regions of Vietnam (Hoc Mon, Tien Giang, and Dak Lak) plays a key role in the diversification of essential oil composition in Piper betel

Vegetative growth and fruit quality of \u27Tahiti\u27 acid lime grafted onto different rootstocks

The production of \u27Tahiti\u27 acid lime in Brazil is currently limited to the northeastern and southeastern regions and research is required to identify compatible rootstocks in order to potentiate other producing areas and to meet the needs of the consumer and industrial markets. The aim of this study was to evaluate the physical characteristics during vegetative growth and the physicochemical attributes and quality of fruit produced by \u27Tahiti CNPMF-02\u27 acid lime grafted onto the twelve rootstocks ‘Cravo LCRSC’ and ‘LCR-03’ lime, citrandarins ‘CTRI’ and ‘CTRSD’, citrumelo ‘CTSW’, ‘Pear HTR-069’ orange, ‘Volkamer LVK×LCR- 038’ lemon, and ‘Sunki TSKC×CTSW-028\u27, ‘TSKC×CTSW-041\u27, ‘TSKC×(LCR×TR)-059\u27, ‘TSKC×TRFD-003\u27 and ‘TSKT’ tangerine. The randomized experimental design included 12 treatments with four repetitions and five plants per plot. The characteristics of the grafted plants were evaluated during vegetative growth at 3.5, 4.0 and 4.5 years of age, while fruit quality was assessed at maturity. All tested rootstocks were compatible with the scion \u27Tahiti CNPMF 02\u27 acid lime cultivated under the climate and soil conditions in the north of Mato Grosso state. The rootstocks ‘LCR-003\u27, ‘CTRI’, ‘CTRSD’, ‘CTSW’ and ‘TSKC×CTSW-028\u27 induced the greatest vertical growth, trunk diameter, crown volume and vigor in the grafted plants. The physicochemical characteristics and quality of the fruit produced by grafted plants of \u27Tahiti CNPMF 02\u27 acid lime met market expectations, except for the parameter total titratable acidity. The fruits produced by \u27Tahiti CNPMF 02\u27 acid lime grafted onto ‘CTRSD’, ‘TSKC×(LCR×TR)-059’, ‘LCR-003’, ‘TSKC×CTSW-041’, ‘TSKC×TRFD-003’, ‘LVK×LCR-038’ and ‘CTRI’ were the largest in terms of length and mass.The production of \u27Tahiti\u27 acid lime in Brazil is currently limited to the northeastern and southeastern regions and research is required to identify compatible rootstocks in order to potentiate other producing areas and to meet the needs of the consumer and industrial markets. The aim of this study was to evaluate the physical characteristics during vegetative growth and the physicochemical attributes and quality of fruit produced by \u27Tahiti CNPMF-02\u27 acid lime grafted onto the twelve rootstocks ‘Cravo LCRSC’ and ‘LCR-03’ lime, citrandarins ‘CTRI’ and ‘CTRSD’, citrumelo ‘CTSW’, ‘Pear HTR-069’ orange, ‘Volkamer LVK×LCR- 038’ lemon, and ‘Sunki TSKC×CTSW-028\u27, ‘TSKC×CTSW-041\u27, ‘TSKC×(LCR×TR)-059\u27, ‘TSKC×TRFD-003\u27 and ‘TSKT’ tangerine. The randomized experimental design included 12 treatments with four repetitions and five plants per plot. The characteristics of the grafted plants were evaluated during vegetative growth at 3.5, 4.0 and 4.5 years of age, while fruit quality was assessed at maturity. All tested rootstocks were compatible with the scion \u27Tahiti CNPMF 02\u27 acid lime cultivated under the climate and soil conditions in the north of Mato Grosso state. The rootstocks ‘LCR-003\u27, ‘CTRI’, ‘CTRSD’, ‘CTSW’ and ‘TSKC×CTSW-028\u27 induced the greatest vertical growth, trunk diameter, crown volume and vigor in the grafted plants. The physicochemical characteristics and quality of the fruit produced by grafted plants of \u27Tahiti CNPMF 02\u27 acid lime met market expectations, except for the parameter total titratable acidity. The fruits produced by \u27Tahiti CNPMF 02\u27 acid lime grafted onto ‘CTRSD’, ‘TSKC×(LCR×TR)-059’, ‘LCR-003’, ‘TSKC×CTSW-041’, ‘TSKC×TRFD-003’, ‘LVK×LCR-038’ and ‘CTRI’ were the largest in terms of length and mass

Growth components and gas exchange in Tecoma stans L. plants under water stress

The reduction in water availability has significant effects on plant physiology and morphology, leading to changes in metabolism that impair the plant establishment in the field. This study aimed to evaluate the effects of water stress on morphophysiological traits of Tecoma stans (L.) Juss. ex Kunth seedlings. The experimental design was conducted in a completely randomized design, with four irrigation intervals: daily irrigation (control), and irrigation intervals of 5, 10, and 15 days. Plant height and leaf number were measured weekly after the 15th day after transplanting and the root length, dry matter, and biomass allocation in different plant organs were determined after 60 days. Gas exchange measurements were also assessed. Tecoma stans showed significant response to water stress due to the extended irrigation intervals, showing a significant reduction in plant height, leaf number, dry matter (stem, leaves, roots, and total dry matter). Photosynthetic rates ranged from 1.8 μmol m⁻² s⁻¹ under the longest irrigation interval to 13.6 μmol m⁻² s⁻¹ in plants irrigated daily, with transpiration rates following a similar behavior. The highest gas exchange rates were observed under daily irrigation. The most significant negative effects on photosynthesis, transpiration, and particularly stomatal conductance were observed in plants irrigated under intervals of 10 and 15 days.The reduction in water availability has significant effects on plant physiology and morphology, leading to changes in metabolism that impair the plant establishment in the field. This study aimed to evaluate the effects of water stress on morphophysiological traits of Tecoma stans (L.) Juss. ex Kunth seedlings. The experimental design was conducted in a completely randomized design, with four irrigation intervals: daily irrigation (control), and irrigation intervals of 5, 10, and 15 days. Plant height and leaf number were measured weekly after the 15th day after transplanting and the root length, dry matter, and biomass allocation in different plant organs were determined after 60 days. Gas exchange measurements were also assessed. Tecoma stans showed significant response to water stress due to the extended irrigation intervals, showing a significant reduction in plant height, leaf number, dry matter (stem, leaves, roots, and total dry matter). Photosynthetic rates ranged from 1.8 μmol m⁻² s⁻¹ under the longest irrigation interval to 13.6 μmol m⁻² s⁻¹ in plants irrigated daily, with transpiration rates following a similar behavior. The highest gas exchange rates were observed under daily irrigation. The most significant negative effects on photosynthesis, transpiration, and particularly stomatal conductance were observed in plants irrigated under intervals of 10 and 15 days