Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments

Luminescent nanoparticles are innovative tools for medicine, allowing the imaging of cells and tissues, and, at the same time, carrying and releasing different types of molecules. We explored and compared the loading/release ability of diclofenac (COX-2 antagonist), in both undoped-and luminescent Terbium3+ (Tb3+)-doped citrate-coated carbonated apatite nanoparticles at different temperatures (25, 37, 40 °C) and pHs (7.4, 5.2). The cytocompatibility was evaluated on two osteosarcoma cell lines and primary human osteoblasts. Biological effects of diclofenac-loadednanoparticles were monitored in an in vitro osteoblast’s cytokine–induced inflammation model by evaluating COX-2 mRNA expression and production of PGE2. Adsorption isotherms fitted the multilayer Langmuir-Freundlich model. The maximum adsorbed amounts at 37 °C were higher than at 25 °C, and particularly when using the Tb3+-doped particles. Diclofenac-release efficiencies were higher at pH 5.2, a condition simulating a local inflammation. The luminescence properties of diclofenac-loaded Tb3+-doped particles were affected by pH, being the relative luminescence intensity higher at pH 5.2 and the luminescence lifetime higher at pH 7.4, but not influenced either by the temperature or by the diclofenac-loaded amount. Both undoped and Tb3+-doped nanoparticles were cytocompatible. In addition, diclofenac release increased COX-2 expression and decreased PGE2 production in an in vitro inflammation model. These findings evidence the potential of these nanoparticles for osteo-localized delivery of anti-inflammatory drugs and the possibility to localize the inflammation, characterized by a decrease in pH, by changes in luminescence

Far Ultraviolet Absolute Flux of alpha Virginis

We present the far ultraviolet spectrum of alpha Virginis taken with EURD spectrograph on-board MINISAT-01. The spectral range covered is from ~900 to 1080 A with 5 A spectral resolution. We have fitted Kurucz models to IUE spectra of alpha Vir and compared the extension of the model to our wavelengths with EURD data. This comparison shows that EURD fluxes are consistent with the prediction of the model within 20-30%, depending on the reddening assumed. EURD fluxes are consistent with Voyager observations but are ~60% higher than most previous rocket observations of alpha Vir.Comment: 13 pages, 4 figures. Submitted to The Astrophysical Journa

EMPLEO DE LA ESPECTROSCOPIA INFRARROJA Y LA DIFRACCION DE RAYOS-X EN LA CARACTERIZACION DE CATALIZADOR DE CRAQUEO FCC SOMETIDO A PROCESO DE DESACTIVACION HIDROTERMICA

Se empleb In espectroscopia infrarroja, la difraccibn de rayos-X y el analisis de microactividad en el seguimiento del proceso de desactivación hidrotermica -a nivel de laboratorio- de un catalizador de craqueo cataiitice FCC dcl tipo REY. En la evaluaci n del proceso de desactivacibn mediante difraccibn dc rayos-X se emplcaron Ins analisis de por ciento de eristalinidad-asociado a la concentración de zeolita taujasita, fase activa del catalizador- y la determinación del parametro de la celda unidad as de la mencionada zeolita. Se observó que el parámetro cristalográfico y disminuye a medida que avanza el proceso de desactivación, lo cual indica que esta ocurriendo la contracción de la celda de la zeolita producto del efecto de desaluminación. en este trabajo se demuestra que el procedimiento analítico utilizado para la determinación del por ciento de cristanilidad por difracciónde rayos-X conduce a una sobrevaloración de este índice cuando en el proceso de desactivación esta presente de manera apreciable la desaluminacibn de la zeolita. En este estudio se propone el empleo de la espectroscopia infrarroja como método alternative para el seguimiento de procesos de desactivación, especificamente, la variación de intensidad de Ias zonas de bandas de absorción presentes entre 400-700 cm-1 que están asociadas a vibraciones de deformación de enlaces- individuales del tetraedro AI,SiO4 y enlaces AI,SiO4  en su conjunto. La mediciones de microactividad reflejan el comportamiento de la desactivación hidrotérmica en función del tiempo de tratamiento y corroboraron los resultados obtenidos por difracción de rayos-X y espectroscopia infrarroja

Post common envelope binaries from the SDSS VI. SDSS J120615.73+510047.0 : a new low accretion rate magnetic binary

We report the discovery of the ninth pre-polar consisting of a late-type ZAMS secondary and a magnetic white dwarf. The white dwarf accretes at an extreme low rate, \dot{M} \sim 10^ $M_\odot$ yr-1, from the wind of the companion donor star. The source was found in our systematic search for WD/MS binaries within SDSS/SEGUE. Based on seven Sloan spectra we estimate a binary period of ~200, 230, or 270 min. The UV to IR spectral energy distribution was decomposed into a dM3-dM4 ZAMS secondary and a cool white dwarf, ~9000 K, which consistently imply a distance between 360 and 420 pc. The optical spectrum displays one pronounced cyclotron hump, likely originating from a low-temperature plasma, ~1 keV, in a field of 108 MG. We comment on the evolutionary link between polars and pre-polars

Immunodiagnosis of Trichinella infection in the horse.

From 1998 to 2000, 5,267 horse sera were collected from several Trichinella regions in Romania. Sera were initially screened in laboratories in Romania, Serbia and Italy with an ELISA and a Western blot (Wb) using an excretory/secretory (ES) antigen and several conjugates (protein A, protein G, and sheep or goat anti-horse). Differences in serology results were obtained among the different conjugates and also between ELISA and Wb. Depending on the test used, specific antibodies were found at a prevalence rate of 3-6 % of horses. Serum samples classified as positive were tested again by ELISA using a synthetic tyvelose glycan-BSA antigen, in Italy. All serum samples tested using this antigen were negative; in contrast, serum samples from experimentally infected horses were positive with the glycan antigen. The negative results obtained with the glycan antigen are consistent with the low prevalence of horse trichinellosis reported in the literature. Based on these results, further studies are needed to validate immunodiagnostic tests to detect Trichinella infection in horses

Far Ultraviolet Spectra of B Stars near the Ecliptic

Spectra of B stars in the wavelength range of 911-1100 A have been obtained with the EURD spectrograph onboard the Spanish satellite MINISAT-01 with ~5 A spectral resolution. IUE spectra of the same stars have been used to normalize Kurucz models to the distance, reddening and spectral type of the corresponding star. The comparison of 8 main-sequence stars studied in detail (alpha Vir, epsilon Tau, lambda Tau, tau Tau, alpha Leo, zeta Lib, theta Oph, and sigma Sgr) shows agreement with Kurucz models, but observed fluxes are 10-40% higher than the models in most cases. The difference in flux between observations and models is higher in the wavelength range between Lyman alpha and Lyman beta. We suggest that Kurucz models underestimate the FUV flux of main-sequence B stars between these two Lyman lines. Computation of flux distributions of line-blanketed model atmospheres including non-LTE effects suggests that this flux underestimate could be due to departures from LTE, although other causes cannot be ruled out. We found the common assumption of solar metallicity for young disk stars should be made with care, since small deviations can have a significant impact on FUV model fluxes. Two peculiar stars (rho Leo and epsilon Aqr), and two emission line stars (epsilon Cap and pi Aqr) were also studied. Of these, only epsilon Aqr has a flux in agreement with the models. The rest have strong variability in the IUE range and/or uncertain reddening, which makes the comparison with models difficult.Comment: 25 pages, 6 figures, to be published in The Astrophysical Journa

Meshless electrophysiological modeling of cardiac resynchronization therapy—benchmark analysis with finite-element methods in experimental data

Computational models of cardiac electrophysiology are promising tools for reducing the rates of non-response patients suitable for cardiac resynchronization therapy (CRT) by optimizing electrode placement. The majority of computational models in the literature are mesh-based, primarily using the finite element method (FEM). The generation of patient-specific cardiac meshes has traditionally been a tedious task requiring manual intervention and hindering the modeling of a large number of cases. Meshless models can be a valid alternative due to their mesh quality independence. The organization of challenges such as the CRT-EPiggy19, providing unique experimental data as open access, enables benchmarking analysis of different cardiac computational modeling solutions with quantitative metrics. We present a benchmark analysis of a meshless-based method with finite-element methods for the prediction of cardiac electrical patterns in CRT, based on a subset of the CRT-EPiggy19 dataset. A data assimilation strategy was designed to personalize the most relevant parameters of the electrophysiological simulations and identify the optimal CRT lead configuration. The simulation results obtained with the meshless model were equivalent to FEM, with the most relevant aspect for accurate CRT predictions being the parameter personalization strategy (e.g., regional conduction velocity distribution, including the Purkinje system and CRT lead distribution). © 2022 by the authors. Licensee MDPI, Basel, Switzerland

The EBLM Project I-Physical and orbital parameters, including spin-orbit angles, of two low-mass eclipsing binaries on opposite sides of the Brown Dwarf limit

This paper introduces a series of papers aiming to study the dozens of low mass eclipsing binaries (EBLM), with F, G, K primaries, that have been discovered in the course of the WASP survey. Our objects are mostly single-line binaries whose eclipses have been detected by WASP and were initially followed up as potential planetary transit candidates. These have bright primaries, which facilitates spectroscopic observations during transit and allows the study of the spin-orbit distribution of F, G, K+M eclipsing binaries through the Rossiter-McLaughlin effect. Here we report on the spin-orbit angle of WASP-30b, a transiting brown dwarf, and improve its orbital parameters. We also present the mass, radius, spin-orbit angle and orbital parameters of a new eclipsing binary, J1219-39b (1SWAPJ121921.03-395125.6, TYC 7760-484-1), which, with a mass of 95 +/- 2 Mjup, is close to the limit between brown dwarfs and stars. We find that both objects orbit in planes that appear aligned with their primaries' equatorial planes. Neither primaries are synchronous. J1219-39b has a modestly eccentric orbit and is in agreement with the theoretical mass--radius relationship, whereas WASP-30b lies above it.Comment: 12 pages, 7 figures, data in appendices, submitted to A&A (taking in account 1st referee report

Anthropometry, dietary intake, physical activity and sitting time patterns in adolescents aged 15-17 years: an international comparison in eight Latin American countries

BACKGROUND: Although there is high prevalence of obesity and other cardiovascular risk factors among Latin American adolescents, there is limited evidence on dietary intake and physical activity (PA) patterns in this population. Therefore, we characterized anthropometry, dietary intake, PA and sitting time (ST) in adolescents aged 15-17 years from eight Latin American countries. METHODS: Six hundred seventy-one adolescents (41.4% girls) from the Latin American Study of Nutrition and Health (ELANS) were included. Nutritional status was classified by four BMI (kg/m2) categories. Waist circumference (WC) was categorized as above or below thresholds. Dietary intake was assessed through two non-consecutive 24-h dietary recalls. PA and ST were measured using the International Physical Activity Questionnaire (IPAQ). We calculated overall and country-specific estimates by sex and tested for differences between boys and girls. RESULTS: Differences in the prevalence of overweightness (15.1 and 21.6%) and obesity (8.5 and 6.5%) between boys and girls, respectively, were statistically insignificant (p = 0.059). Average energy intake was 2289.7 kcal/day (95% CI: 2231-2350) for boys and 1904.2 kcal/day (95% CI: 1840-1963) for girls (p  0.05 for all outcomes). There was no statistically significant difference in the prevalence of total energy (TE) saturated fat and added sugar (>10% of TE) between girls and boys (49.6% versus 44.8 and 81.7% versus 76.1%, respectively). Prevalence of physical inactivity was 19% in boys and 43.7% in girls (p  0.05). CONCLUSIONS: These findings highlight the high prevalence of poor dietary intake and physical inactivity in adolescents from Latin American countries. Therefore, effective and sustainable strategies and programmes are needed that promote healthier diets, regular PA and reduce ST among Latin American adolescents. TRIAL REGISTRATION: Clinical Trials NCT02226627. Retrospectively registered on August 27, 2014