Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

Effects of Alpha Particle and Proton Beam Irradiation as Putative Cross-Talk between A549 Cancer Cells and the Endothelial Cells in a Co-Culture System.

High-LET ion irradiation is being more and more often used to control tumors in patients. Given that tumors are now considered as complex organs composed of multiple cell types that can influence radiosensitivity, we investigated the effects of proton and alpha particle irradiation on the possible radioprotective cross-talk between cancer and endothelial cells

A ceux qui s'interrogent sur les compétences et leur évaluation

info:eu-repo/semantics/publishe

Population genetics and dynamics of the black truffle in a man-made truffle field

The colonization dynamics of the black true in an arti®cial ®eld were assessed through analyses of microsatellite and RAPD markers. The true ®eld was composed of three tree species and mycelial inoculum of three di€erent origins, and was monitored for the ®rst three years of true production. We found very low levels of genetic diversity. Isolation by distance was detected only at the between- tree level. This could be interpreted as local colonization around each tree facilitated by the presence of the tree root system. At the larger spatial scale of the European range, the absence of isolation by distance corroborates the hypothesis of an impact of glaciation on genetic variation, followed by rapid postglaciation demographic expansion. In addition, genetic variation of harvested trues was explained by neither inoculation origin, nor tree species. Our study questions the real impact of man- made inoculation of tree root systems with fungal mycelia

First results from the INDRA-FAZIA apparatus on isospin diffusion in 58,64^{58,64}Ni+58,64^{58,64}Ni systems at Fermi energies

International audienceAn investigation of the isospin equilibration process in the reactions $^{58,64}$Ni+$^{58,64}$Ni at two bombarding energies in the Fermi regime ($32\,$MeV/nucleon and $52\,$MeV/nucleon) is presented. Data have been acquired during the first experimental campaign of the coupled INDRA-FAZIA apparatus in GANIL. Selecting from peripheral to semi-central collisions, both the neutron content of the quasiprojectile residue and that of the light ejectiles coming from the quasiprojectile evaporation have been used as probes of the dynamical process of isospin diffusion between projectile and target for the asymmetric systems. The isospin transport ratio technique has been employed. The relaxation of the initial isospin imbalance with increasing centrality has been clearly evidenced. The isospin equilibration appears stronger for the reactions at $32\,$MeV/nucleon, as expected due to the longer projectile-target interaction time than at $52\,$MeV/nucleon. Coherent indications of isospin equilibration come from the quasiprojectile residue characteristics and from particles ascribed to the quasiprojectile decay

First results from the INDRA-FAZIA apparatus on isospin diffusion in Ni 58,64 + Ni 58,64 systems at Fermi energies

An investigation of the isospin equilibration process in the reactions Ni58,64+Ni58,64 at two bombarding energies in the Fermi regime (32 and 52 MeV/nucleon) is presented. Data have been acquired during the first experimental campaign of the coupled INDRA-FAZIA apparatus in GANIL. Selecting from peripheral to semicentral collisions, both the neutron content of the quasiprojectile residue and that of the light ejectiles coming from the quasiprojectile evaporation have been used as probes of the dynamical process of isospin diffusion between projectile and target for the asymmetric systems. The isospin transport ratio technique has been employed. The relaxation of the initial isospin imbalance with increasing centrality has been clearly evidenced. The isospin equilibration appears stronger for the reactions at 32 MeV/nucleon, as expected due to the longer projectile-target interaction time than at 52 MeV/nucleon. Coherent indications of isospin equilibration come from the quasiprojectile residue characteristics and from particles ascribed to the quasiprojectile decay