Manual de producción del sistema acuaponía del Centro Agroempresarial y Acuícola

El presente, es un manual de producción del sistema acuapónico de la unidad productiva acuícola del centro Agroempresarial y acuícola de la Regional Guajira, realizada como instrumento, que recopila la documentación de procedimientos que conforman las actividades ejecutadas en el sistema acuaponia del centro de formación, además, sirve como guía para aprendices, instructores, productores y población en general, sobre el desarrollo de la producción acuícola bajo el enfoque acuapónico.Generalidades -- Antecedentes -- Acuicultura -- Hidroponía --. Acuaponía -- Descripción del sistema acuaponía de la unidad productiva acuícola del CAA -- Procedimiento para la producción de especies animales “Peces” -- Mantenimiento, limpieza y desinfección de los tanques de producción -- Siembra de Peces -- Nutrición y suministro de alimento -- Cosecha, sacrificio y comercialización de la producción -- Procedimiento para la producción de especies vegetales -- Preparación de las camas hidropónicas y sistema NFT -- Instalación y condiciones de invernadero -- Siembra y mantenimiento de las semillas -- Trasplante de especies vegetales al sistema hidropónico -- Mantenimiento de especies vegetales -- Análisis del desarrollo vegetal y recolección de frutos -- Parámetros de operación del sistema acuapónico -- Controles aplicados en el sistema acuaponía -- Medición de parámetros del agua -- Aplicación de sal marina -- Melaza como medida de control -- Resultados -- Producción de especies animales -- Producción de especies vegetalesna49 página

Uma nova abordagem para entender a escolha modal e de rotas dos pedestres em Portugal

O presente trabalho visa analisar os fatores que interferem nas escolhas que as pessoas fazem de modos de transporte ou caminhos a pé, tendo em conta quatro grupos de variáveis: geométricos, sintáticos, uso do solo e acessibilidade de transportes. Para isso, foi formulado um modelo de escolha discreta em duas partes: escolha do modo e escolha de caminhos (a pé). Os resultados mostraram que fatores como segurança, conforto e forma urbana contribuem significativamente para a escolha dos caminhos. Para a escolha do modo, a maioria dos fatores determinantes foram: tempo e períodos do dia, sendo que ambos são exemplos de fatores que não são controláveis; e elementos econômicos e o tempo de deslocamento. Para a escolha da bicicleta, os principais fatores foram a presença de ciclovias e bicicletários. Foi possível concluir que o carro é o modo de transporte com maior preferência natural, seguida de caminhada e finalmente bicicleta

Oral and dental health status in patients with eating disorders in Madrid, Spain

The aim of the present study was to describe and compare the oral and dental health status of two groups, one diagnosed with eating disorders (EDs), and another group without this pathology, assessing the following oral manifestations: dental alterations, periodontal disorders, soft tissue disorders, non-stimulated salivary flow, and oral pH. This comparative transversal epidemiological study included 179 participants, of whom 59 were diagnosed with EDs (Eating Disorder Group: EDG) and 120 had no antecedents of EDs (No Eating Disorder Group: NEDG). All patients fulfilled the following inclusion criteria: women aged over 18 years, diagnosed with an ED by a specialist, patients who had undergone at least 1 year monitoring by the Clinical Nutrition Unit, and had not received any periodontal treatment during the previous 6 months. Both groups were homogeneous in terms of sex, age, education, and socioeconomic level. Oral exploration was performed, registering clinical variables, as well as sociodemographic and socioeconomic data, oral hygiene habits, and smoking. Statistical significance was established as p 95%). The dental erosion (DE) was the most significative feature of dental alterations. The degree of DE was significantly greater in the EDG (p<0.001). A significant association between soft tissue lesions and EDs was found (p<0.001) A notable difference in non-stimulated salivary flow was found between the groups (p<0.001). No significant differences between the groups were found for periodontal status, dental caries, or oral hygiene practices. On the basis of the results obtained, it is necessary to carry out oral/dental examination as soon as an ED is diagnosed with regular check-ups thereafter

Effect of plant extracts on the inhibition of the mycelial growth of Penicilium citrinum Link

Objective: Evaluate in vitro plant extracts of three plant species: mistletoe (Psittacanthus spp.), skunk grass (Petiveria alliacea) and ginger (Zingiber officinale), to determine the inhibitory effect on mycelial growth of Penicilliun citrinum isolated from coffee beans. Design/methodology/approach: Filtrations were performed under aseptic conditions using a vacuum system. These were added to the Potato Dextrose Agar medium, once solidified a 5 mm disc of P. citrinum was placed in the center of the Petri dish. Results: The ethyl plant extracts showed inhibition of (100%) on the development of the pathogen as well as the chemical product. Findings/conclusions: Ethyl plant extracts can be an agroecological alternative to control P. citrinum.Objective: To evaluate in vitro plant extracts of three plant species —mistletoe (Psittacanthus spp.), guinea hen weed (Petiveria alliacea), and ginger (Zingiber officinale)— with the aim of determining their inhibitory effect on the mycelial growth of Penicilliun citrinum isolated from coffee beans.Design/Methodology/Approach: Filtrations were carried out under aseptic conditions using a vacuum system and were added to the Potato Dextrose Agar medium. Once it had solidified, a 5-mm disc of P. citrinum was placed in the center of the Petri dish.Results: The ethyl plant extracts, like the chemical product, showed a 100% inhibition on the pathogen development.Findings/Conclusions: Ethyl plant extracts can be an agroecological alternative for the control of P. citrinum

TRATAMIENTO AL CONTENIDO DEL ÁREA DE LAS CIENCIAS NATURALES PARA FAVORECER LA RESOLUCIÓN DE PROBLEMAS

It was presented how deep changes are evidenced in economy, politics, and the scientific knowledge at present times. Wherefore the man is called to adapt and to fight to save the planet and the species, fundamentally the human one. In this aspect it plays an essential role the knowledge of the contents of the subjects of the area of the Natural Sciences in the primary school. They possess wide potentialities to contribute to this pretense, keeping in mind that besides instructing the children in the appropriate interpretation of the phenomena and processes that happen in nature and society, they favor a general culture on the repercussion and the man's sustainable action on nature, as well as the necessity of their care and conservation.Se abordó como en la actualidad se evidencian profundos cambios en la economía, la política y el conocimiento científico donde el hombre está llamado a adaptarse y a luchar por salvar el planeta y las especies, fundamentalmente la humana. En este aspecto juega un papel esencial el tratamiento a los contenidos de las asignaturas del área de las Ciencias Naturales en la escuela primaria, los que poseen amplias potencialidades para el desarrollo de los mismos a partir de la resolución de problemas, y de esta forma contribuir a esta pretensión, teniendo en cuenta que además de instruir a los escolares en la adecuada interpretación de los fenómenos y procesos que ocurren en la naturaleza y la sociedad, también propicia una cultura general sobre la repercusión y la acción sostenible del hombre sobre la naturaleza, así como la necesidad de su cuidado y conservación

Versatile Graphene-Based Platform for Robust Nanobiohybrid Interfaces

Technologically useful and robust graphene-based interfaces for devices require the introduction of highly selective, stable, and covalently bonded functionalities on the graphene surface, whilst essentially retaining the electronic properties of the pristine layer. This work demonstrates that highly controlled, ultrahigh vacuum covalent chemical functionalization of graphene sheets with a thiol-terminated molecule provides a robust and tunable platform for the development of hybrid nanostructures in different environments. We employ this facile strategy to covalently couple two representative systems of broad interest: metal nanoparticles, via S-metal bonds, and thiol-modified DNA aptamers, via disulfide bridges. Both systems, which have been characterized by a multi-technique approach, remain firmly anchored to the graphene surface even after several washing cycles. Atomic force microscopy images demonstrate that the conjugated aptamer retains the functionality required to recognize a target protein. This methodology opens a new route to the integration of high-quality graphene layers into diverse technological platforms, including plasmonics, optoelectronics, or biosensing. With respect to the latter, the viability of a thiol-functionalized chemical vapor deposition graphene-based solution-gated field-effect transistor array was assessed

Tumor P70S6K hyperactivation is inversely associated with tumor-infiltrating lymphocytes in triple-negative breast cancer

Purpose: Triple-negative breast cancer (TNBC) is characterized by large heterogeneity and relative lack of available targeted therapies. To find therapeutic strategies for distinct patients with TNBC, several approaches have been used for TNBC clustering, including recently immune and phosphoproteomic patterns. Based on 70-kDa ribosomal protein S6 kinase (P70S6K)-TNBC clustering, the current study explores the immune profiling in TNBC tumors. Methods: Stromal tumor-infiltrating lymphocytes (sTILs) were evaluated in human TNBC tumor samples. Furthermore, immunohistochemistry staining for CD8, CD4, Foxp3, and CD20 was performed in tissue microarrays (TMA) sections. Results: Histological analysis showed decreased sTILs, CD20+ cells, and CD8+/CD4+ ratio in high phosphorylated P70S6K (p-P70S6K) tumors. Moreover, p-P70S6K score was directly correlated with CD4+ and Foxp3+ T cells, while it was inversely correlated with CD8+/CD4+ and CD8+/Foxp3+ ratios. Conclusion: sTIL infiltration and lymphocyte profiling vary in the context of hyperactivation of P70S6K in TNBC tumorsThe project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 893597. RC is a recipient of the ISCIII grants: PI17/01865 and PI20/01458. MQF is a recipient of the following Grants: AES-PI19/00454 funded by the ISCIII and co-funded by the European Regional Development Fund (ERDF) and B2017/BMD3733 (Immunothercan-CM)—Call for Coordinated Research Groups from Madrid Region—Madrid Regional Government—ERDF funds. The study was also funded by CRIS Contra el Cancer Foundatio

Recensiones [Revista de Historia Económica Año X Otoño 1992 n. 3 pp. 519-548]

Editada en la Universidad Carlos IIIFranco Amatori y Bruno Bezza. Montecatini 1888-1966. Capitoli di storia di una grande impresa. (Por Núria Puig).-- Dudley Baines. Emigration from Europe, 1815-1930. (Por Blanca Sánchez Alonso).-- Roderick Floud, Kenneth Wachter y Annabel Gregory. Height, Health and History. Nutritional Status in the United Kingdom, 1750-1980. (Por José Miguel Martínez Carrión).-- G. Goertz. The World Chemical Industry around 1910: a Comparative Analysis by Branch and Country. (Por Núria Puig).-- José Luis García Delgado, ed.. Las ciudades en la modernización de España. Los decenios interseculares. VIII Coloquio de Historia Contemporánea de España dirigido por Manuel Tuñón de Lara. (Por Luis Garrido González).-- Charles P. Kindleberger. Manias, Panics and Crashes. A History of Financial Crisis. (Por J. Ignacio Peña).-- Carlos Alberto Mayo. Los Betlemitas en Buenos Aires: Convento, Economía y Sociedad (1748-1822). (Por Carlos Newland).-- Hilda Sábato. Agrarian Capitalism and the World Market: Buenos Aires in the Pastoral Age, 1840-1890. (Por Julio E. Revilla).-- Gabriel Tortella, ed.. Educación y Desarrollo Económico desde la Revolución Industrial. (Por Cecilia Albert)Publicad

Arabidopsis immune responses triggered by cellulose‐ and mixed‐linked glucan‐derived oligosaccharides require a group ofleucine‐rich repeat malectinreceptor kinases

[EN] The plant immune system perceives a diversity of carbohydrate ligands from plant and microbial cell walls through the extracellular ectodomains (ECDs) of pattern recognition receptors (PRRs), which activate pattern-triggered immunity (PTI). Among these ligands are oligosaccharides derived from mixed-linked b- 1,3/b-1,4-glucans (MLGs; e.g. b-1,4-D-(Glc)2-b-1,3-D-Glc, MLG43) and cellulose (e.g. b-1,4-D-(Glc)3, CEL3). The mechanisms behind carbohydrate perception in plants are poorly characterized except for fungal chitin oligosaccharides (e.g. b-1,4-D-(GlcNAc)6, CHI6), which involve several receptor kinase proteins (RKs) with LysM-ECDs. Here, we describe the isolation and characterization of Arabidopsis thaliana mutants impaired in glycan perception (igp) that are defective in PTI activation mediated by MLG43 and CEL3, but not by CHI6. igp1–igp4 are altered in three RKs – AT1G56145 (IGP1), AT1G56130 (IGP2/IGP3) and AT1G56140 (IGP4) – with leucine-rich-repeat (LRR) and malectin (MAL) domains in their ECDs. igp1 harbors point mutation E906K and igp2 and igp3 harbor point mutation G773E in their kinase domains, whereas igp4 is a T-DNA insertional loss-of-function mutant. Notably, isothermal titration calorimetry (ITC) assays with purified ECDRKs of IGP1 and IGP3 showed that IGP1 binds with high affinity to CEL3 (with dissociation constant KD = 1.19 0.03 lM) and cellopentaose (KD = 1.40 0.01 lM), but not to MLG43, supporting its function as a plant PRR for cellulose-derived oligosaccharides. Our data suggest that these LRR-MAL RKs are components of a recognition mechanism for both cellulose- and MLG-derived oligosaccharide perception and downstream PTI activation in Arabidopsis.SIGrant PID-2021-126006OB-100 from the Spanish Ministry of Science and Innovation to AMThis work has also been financially supported by the ‘Severo Ochoa (SO) Programme for Centres of Excellence in R&D’ from the Agencia Estatal de Investigaci on (AEI) of Spain (grants SEV-2016-0672 (2017-2021) and CEX2020-000999-S (2022-2025) to the CBGP). In the frame of the SO program, HM and PF-C were supported with postdoctoral fellowships. MM-D, DJB and DR were recipients of PhD Fellows PRE2019-088120 and PRE2019-091276 (SEV-2016- 0672) from AEI, and IND2017/BIO-7800 from Madrid Regional Government, respectively. Research in the lab of JS was financially supported by the University of Lausanne, the European Research Council (ERC) (grant agreement no. 716358) and the Swiss National Science Foundation (grant no. 310030_204526)

Impacto do desenho da malha viária na mobilidade urbana

The paper analyzes the performance of different street plans to determine which variables are present in each type, as first step to creating a walkability index. Three neighborhoods of Lisbon are used as case studies: (a) Campo de Ourique - with a grid street plan - (b) Graça ”“ with irregular roads - and (c) Telheiras - with a contemporary street plan. It was found that the grid-based street plan has more road homogeneity, a lower straightness index and a higher average integration index. The contemporary street plan performs the worst, while the organic street plan is ranked in the middle.&nbsp;O artigo explora o desempenho de diferentes malhas viárias de modo a apresentar quais variáveis estão mais presentes em cada tipo de malha criando diretrizes para a composição de um índice de caminhabilidade. São utilizados três bairros de Lisboa como estudos de caso: (a) Campo de Ourique ”“ cuja malha é regular ”“ (b) Graça ”“ caracterizado pela irregularidade ”“ e (c) Telheiras ”“ cujo desenho apresenta características contemporâneas. Verificou-se que a malha regular apresenta maior homogeneidade viária, menor índice de conversão e maior média de integração, enquanto a malha contemporânea apresentou o pior desempenho e a malha orgânica, por sua vez, uma posição intermediária