Are pan-immune-inflammation value, systemic inflammatory response index and other hematologic inflammatory indexes clinically useful to predict first-trimester pregnancy loss

The purpose of the study is to investigate the importance of hematological inflammatory parameters in predicting pregnancy loss in the first trimester. Material and Methods: This study was conducted retrospectively between February and May 2022 in Aksaray University Training and Research Hospital, Obstetrics and gynecology outpatient clinic. Fifty-five of 110 patients diagnosed with first trimester pregnancy loss were included in the study. The control group consisted of 55 pregnant women with first trimester pregnancies. Results: When the whole groups were compared, no statistically significant difference was observed in terms of hemoglobin, platelet, neutrophil, lymphocyte count, neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), lymphocyte-monocyte ratio (LMR), derived NLR ratio (dNLR), systemic inflammatory index (SII), systemic inflammatory response index (SIRI) and pan-immune-inflammation value (PIV). Discussion: NLR, PLR, LMR, dNLR, SII, SIRI and PIV parameters were not associated with first trimester abortions. In this study, unlike previous studies, no hematological parameters, including the pan-immune-inflammation value, were found to have a role in predicting first trimester pregnancy loss

Salicylic acid-induced germination, biochemical and developmental alterations in rye (Secale cereale L.)

Salicylic acid (SA) is one of the endogenous plant growth regulators that modulate various metabolic and physiological events. To evaluate the exogenous SA-induced germination, biochemical and developmental alterations, different concentrations (10, 100, 500 and 1000 μM) of SA were applied to rye (Secale cereale L.) seeds in hydroponic culture conditions for 15 days. The observations revealed that seed germination and root elongation were stimulated in 10 μM SA treatment, however they were inhibited in higher concentrations (100 and 500 μM) of SA. Furthermore, there was no germination in 1000 μM SA. The analysis of antioxidant enzymes revealed that although superoxide dismutase activity increased, catalase activity decreased in comparison to control. Besides, lipid peroxidation and peroxidase activity increased in 10 μM SA, whereas they decreased in higher concentrations. Similarly total chlorophyll content increased in 10 μM SA, but it decreased in 100 and 500 μM SA treatments. Moreover anthocyanins and carotenoids increased after SA treatment. In conclusion, exogenous SA application causes developmental and biochemical alterations in rye

Etkinlik çizelgeleri otizm spektrum bozukluğu olan bireyleri nasıl etkiliyor?Bir sistematik derleme ve meta-analiz

: The main purpose of this study was to systematically review and meta-analyse the single-case studies on the use of activity schedules with individuals with autism spectrum disorders. The other purposes were to describe the characteristics of activity schedule studies, assess the design standards of activity schedule studies, estimate the treatment effect of activity schedules, and determine whether activity schedule is an evidence-based practice for children with autism spectrum disorders in improving appropriate behaviours. The non-overlap of all pairs was used to analyse the treatment effect measure. According to the results, seven of 32 activity schedule studies met the design standards with and without reservation. Twenty-four children with autism spectrum disorders whose ages ranged between 3 and 17 years participated in the studies. The target behaviours were on task, independent transition, appropriate peer-play, and following schedule. The photographs and texts were used in both traditional and innovative activity schedules. The overall non-overlap of all pairs score shows that the activity schedule strongly affects those participants with autism spectrum disorders. Finally, this study indicates that the activity schedules can be recommended as an evidence-based practice to improve the appropriate behaviours of children with autism spectrum disorders.Bu çalışmanın amacı, etkinlik çizelgelerinin otizm spektrum bozukluğu olan bireylerle kullanımına ilişkin tek denekli çalışmaların sistematik derlemesini ve meta-analizini yapmaktır. Ayrıca, etkinlik çizelgesi çalışmalarının özelliklerini tanımlamak, desen standartlarını değerlendirmek, etkinlik çizelgelerinin etki büyüklüğünü belirlemek ve etkinlik çizelgelerinin otizm spektrum bozukluğu olan bireyler için uygun davranışları geliştirmede kanıta-dayalı bir uygulama olup olmadığını ortaya koymak amaçlanmıştır. Müdahalenin etki büyüklüğünü analiz etmek için tüm örtüşmeyen çiftler kullanılmıştır. Bulgular, 32 çalışmadan yedisinin desen standartlarını koşulsuz ve koşullu karşıladığını göstermektedir. Çalışmalara yaşları 3 ile 17 arasında değişen, otizm spektrum bozukluğu tanısı almış 24 birey katılmıştır. Hedef davranışlar; etkinlikle ilgili olma, bağımsız geçiş, akranıyla uygun oyun oynama ve çizelgeyi izlemedir. Hem geleneksel hem de yenilikçi etkinlik çizelgelerinde fotoğraflar ve metinler kullanılmıştır. Tüm örtüşmeyen çiftlere ilişkin genel puan, etkinlik çizelgelerinin otizm spektrum bozukluğu olan katılımcılar için güçlü bir etkisi olduğunu göstermiştir. Son olarak bu çalışma, otizm spektrum bozukluğu olan bireylerin uygun davranışlarını geliştirmek için kanıta-dayalı bir uygulama olarak etkinlik çizelgelerinin önerilebileceğini göstermektedir

A rare neurodegenerative disorder with a novel mutation in ROGDI and Rett- like phenotype: Kohlschutter- Tönz syndrome

Kohlschutter-Tonz syndrome (KTZS) is a rare neurodegenerative disorder that presents with seizures, developmental delay, psychomotor regression, hypoplastic dental enamel morphology characteristic for amelogenesis imperfecta, and dysmorphologies. Genetic analysis has identified loss of function mutations within the coding region of the ROGDI and SLC13A5 genes in KTZS. In this report, we documented the clinical, radiological, electroencephalographic, and genetic results of a 3.5-year-old Turkish girl, born to nonconsanguineous parents, who was the first patient diagnosed with KTZS in Turkey. The patient presented with Rett syndrome-like phenotype, neurodevelopmental delay, refractory seizures, and amelogenesis imperfecta. After obtaining informed consent, chromosomal DNA was extracted from the peripheral blood of our patient and her parents. To investigate the molecular diagnosis of the patient, the clinical exome sequencing was performed. The Sanger sequencing analysis was performed for all of the family members for the validation and segregation of this mutation. Pub Med/Medline, Web of Science, and Google Scholar were also searched to find all of the published data on KTZS. The literature comprises 18 published studies about KTZS. The genetic analysis of our patient revealed a novel homozygous c.201-1G>T mutation in the ROGDI gene. The same mutation was also found to be heterozygous in her mother and father. The mutation caused alternative splicing of the ROGDI translation and resulted in a disruption of the ROGDI protein

Synthesis, structural characterization and anticancer activity of 3- (3,5-dinitrobenzoyl)-1H-imidazolidine-2-thione

201-209The present study describes the synthesis, characterization and in vitro anticancer potential evaluation of a novel 3-(3,5-dinitrobenzoyl)-1H-imidazolidine-2-thione compound. In the first step, structure analysis of the compound has been elucidated by NMR and FT-IR techniques. Theoretical NMR, FT-IR spectra, HOMO and LUMO orbital energies and MEP analyses have been used to determine the activity of the molecule by Gaussian 09 package program using DFT techniques. Furthermore, docking calculations have been performed for the BRCA2 (PDB Code: 3EU7) active side to foresee the possible mechanism of action of the synthesized compound. In the second step of the study, the synthesized compound has been screened for its potential in vitro anticancer activity against MCF-7 human breast cancer cell line using the cell proliferation (XTT), apoptosis, cell cycle arrest and intracellular ROS production assays. The results of XTT test revealed significant dereases in MCF-7 cell viability with the 24h IC50 value of 7.57 μM. It has been also observed that treatment with the IC50 concentration of the compound can significantly induce apoptosis, intracellular ROS production and G2/M phase arrest in MCF-7 cells

Shifts in appraisal dimensions as mediators of efficiency of reappraisal in emotion regulation

Üks viis oma emotsiooni reguleerida on kasutada ümberhindamist ehk muuta oma tõlgendust emotsiooni tekitanud olukorrast. Kuigi tõlgendusdimensioonide rolli ümberhindamises on ammu teadvustatud, pole seda ideed ümberhindamise uurimisel seni kasutatud. Käesoleva uurimistöö eesmärk on uurida, kas ja kuivõrd võivad muutused tõlgendusdimensioonidel vahendada ümberhindamise mõju emotsiooni regulatsioonile. Igapäevaelus ettetulevate emotsioonide reguleerimise mõõtmiseks kasutati 7 päeva jooksul kogemuse väljavõtte meetodit. Tekstisõnumiga teavitades paluti uuritavatel kella 11, 13 ja 15 ajal täita küsimustiku päevane osa ning kell 19 küsimustiku õhtune kokkuvõtlik osa. Regressioonanalüüsidega uuriti tõlgendusdimensioonide muutuse ja emotsiooni muutuse vahelisi seoseid ning medieerimisanalüüsiga uuriti, kas ja kuivõrd tõlgendusdimensioonide muutused vahendavad ümberhindamise kasutamise mõju emotsioonide muutusele. Uuringus osales 181 inimest vanuses 18-52 aastat ( M =28.25; SD =7.75), kellest 85% olid naised. Saadud tulemused kinnitasid, et kolme analüüsitud emotsiooni - viha, ärevuse, lõõgastuse - muutused on arvestatavas osas seletatavad muutustega kindluse, olulisuse, eesmärgipärasuse, vastutuse ja kontrolli tõlgendusdimensioonidel. Tulemused näitavad ka, et eesmärgipärasus on universaalne tõlgendusdimensioon, mille hinnang tõuseb nii negatiivse emotsiooni vähendamisel kui ka positiivse emotsiooni suurendamisel. Medieerimisanalüüsid näitasid ainult lõõgastuse puhul, et tõlgendusdimensioonid vahendavad ümberhindamise kasutamise ja emotsiooni muutuse vahelist seost ning seda läbi muutuste eesmärgipärasuse, vastutuse ja kontrolli tõlgendusdimensioonidel. Käesolev uuring demonstreerib tõlgendusdimensioonide väärtust ümberhindamise käigus toimuvate kognitiivsete protsesside mõistmisel. Kuna tulemused on eripalgelised, vajab küsimus täiendavat uurimist

Risk factors associated with adverse fetal outcomes in pregnancies affected by Coronavirus disease 2019 (COVID-19): a secondary analysis of the WAPM study on COVID-19.

Objectives To evaluate the strength of association between maternal and pregnancy characteristics and the risk of adverse perinatal outcomes in pregnancies with laboratory confirmed COVID-19. Methods Secondary analysis of a multinational, cohort study on all consecutive pregnant women with laboratory-confirmed COVID-19 from February 1, 2020 to April 30, 2020 from 73 centers from 22 different countries. A confirmed case of COVID-19 was defined as a positive result on real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay of nasal and pharyngeal swab specimens. The primary outcome was a composite adverse fetal outcome, defined as the presence of either abortion (pregnancy loss before 22 weeks of gestations), stillbirth (intrauterine fetal death after 22 weeks of gestation), neonatal death (death of a live-born infant within the first 28 days of life), and perinatal death (either stillbirth or neonatal death). Logistic regression analysis was performed to evaluate parameters independently associated with the primary outcome. Logistic regression was reported as odds ratio (OR) with 95% confidence interval (CI). Results Mean gestational age at diagnosis was 30.6+/-9.5 weeks, with 8.0% of women being diagnosed in the first, 22.2% in the second and 69.8% in the third trimester of pregnancy. There were six miscarriage (2.3%), six intrauterine device (IUD) (2.3) and 5 (2.0%) neonatal deaths, with an overall rate of perinatal death of 4.2% (11/265), thus resulting into 17 cases experiencing and 226 not experiencing composite adverse fetal outcome. Neither stillbirths nor neonatal deaths had congenital anomalies found at antenatal or postnatal evaluation. Furthermore, none of the cases experiencing IUD had signs of impending demise at arterial or venous Doppler. Neonatal deaths were all considered as prematurity-related adverse events. Of the 250 live-born neonates, one (0.4%) was found positive at RT-PCR pharyngeal swabs performed after delivery. The mother was tested positive during the third trimester of pregnancy. The newborn was asymptomatic and had negative RT-PCR test after 14 days of life. At logistic regression analysis, gestational age at diagnosis (OR: 0.85, 95% CI 0.8-0.9 per week increase; pPeer reviewe

Diagnosis of comorbid migraine without aura in patients with idiopathic/genetic epilepsy based on the gray zone approach to the International Classification of Headache Disorders 3 criteria

BackgroundMigraine without aura (MwoA) is a very frequent and remarkable comorbidity in patients with idiopathic/genetic epilepsy (I/GE). Frequently in clinical practice, diagnosis of MwoA may be challenging despite the guidance of current diagnostic criteria of the International Classification of Headache Disorders 3 (ICHD-3). In this study, we aimed to disclose the diagnostic gaps in the diagnosis of comorbid MwoA, using a zone concept, in patients with I/GEs with headaches who were diagnosed by an experienced headache expert.MethodsIn this multicenter study including 809 consecutive patients with a diagnosis of I/GE with or without headache, 163 patients who were diagnosed by an experienced headache expert as having a comorbid MwoA were reevaluated. Eligible patients were divided into three subgroups, namely, full diagnosis, zone I, and zone II according to their status of fulfilling the ICHD-3 criteria. A Classification and Regression Tree (CART) analysis was performed to bring out the meaningful predictors when evaluating patients with I/GEs for MwoA comorbidity, using the variables that were significant in the univariate analysis.ResultsLonger headache duration (<4 h) followed by throbbing pain, higher visual analog scale (VAS) scores, increase of pain by physical activity, nausea/vomiting, and photophobia and/or phonophobia are the main distinguishing clinical characteristics of comorbid MwoA in patients with I/GE, for being classified in the full diagnosis group. Despite being not a part of the main ICHD-3 criteria, the presence of associated symptoms mainly osmophobia and also vertigo/dizziness had the distinguishing capability of being classified into zone subgroups. The most common epilepsy syndromes fulfilling full diagnosis criteria (n = 62) in the CART analysis were 48.39% Juvenile myoclonic epilepsy followed by 25.81% epilepsy with generalized tonic-clonic seizures alone.ConclusionLonger headache duration, throbbing pain, increase of pain by physical activity, photophobia and/or phonophobia, presence of vertigo/dizziness, osmophobia, and higher VAS scores are the main supportive associated factors when applying the ICHD-3 criteria for the comorbid MwoA diagnosis in patients with I/GEs. Evaluating these characteristics could be helpful to close the diagnostic gaps in everyday clinical practice and fasten the diagnostic process of comorbid MwoA in patients with I/GEs