Numerical Simulations of Highly Porous Dust Aggregates in the Low-Velocity Collision Regime

A highly favoured mechanism of planetesimal formation is collisional growth. Single dust grains, which follow gas flows in the protoplanetary disc, hit each other, stick due to van der Waals forces and form fluffy aggregates up to centimetre size. The mechanism of further growth is unclear since the outcome of aggregate collisions in the relevant velocity and size regime cannot be investigated in the laboratory under protoplanetary disc conditions. Realistic statistics of the result of dust aggregate collisions beyond decimetre size is missing for a deeper understanding of planetary growth. Joining experimental and numerical efforts we want to calibrate and validate a computer program that is capable of a correct simulation of the macroscopic behaviour of highly porous dust aggregates. After testing its numerical limitations thoroughly we will check the program especially for a realistic reproduction of various benchmark experiments. We adopt the smooth particle hydrodynamics (SPH) numerical scheme with extensions for the simulation of solid bodies and a modified version of the Sirono porosity model. Experimentally measured macroscopic material properties of silica dust are implemented. We calibrate and test for the compressive strength relation and the bulk modulus. SPH has already proven to be a suitable tool to simulate collisions at rather high velocities. In this work we demonstrate that its area of application can not only be extended to low-velocity experiments and collisions. It can also be used to simulate the behaviour of highly porous objects in this velocity regime to a very high accuracy.The result of the calibration process in this work is an SPH code that can be utilised to investigate the collisional outcome of porous dust in the low-velocity regime.Comment: accepted by Astronomy & Astrophysic

The Physics of Protoplanetesimal Dust Agglomerates. IV. Towards a Dynamical Collision Model

Recent years have shown many advances in our knowledge of the collisional evolution of protoplanetary dust. Based on a variety of dust-collision experiments in the laboratory, our view of the growth of dust aggregates in protoplanetary disks is now supported by a deeper understanding of the physics involved in the interaction between dust agglomerates. However, the parameter space, which determines the collisional outcome, is huge and sometimes inaccessible to laboratory experiments. Very large or fluffy dust aggregates and extremely low collision velocities are beyond the boundary of today's laboratories. It is therefore desirable to augment our empirical knowledge of dust-collision physics with a numerical method to treat arbitrary aggregate sizes, porosities and collision velocities. In this article, we implement experimentally-determined material parameters of highly porous dust aggregates into a Smooth Particle Hydrodynamics (SPH) code, in particular an omnidirectional compressive-strength and a tensile-strength relation. We also give a prescription of calibrating the SPH code with compression and low-velocity impact experiments. In the process of calibration, we developed a dynamic compressive-strength relation and estimated a relation for the shear strength. Finally, we defined and performed a series of benchmark tests and found the agreement between experimental results and numerical simulations to be very satisfactory. SPH codes have been used in the past to study collisions at rather high velocities. At the end of this work, we show examples of future applications in the low-velocity regime of collisional evolution.Comment: accepted by The astrophysical Journa

Neutralization of SARS-CoV-2 by highly potent, hyperthermostable, and mutation-tolerant nanobodies

Monoclonal anti-SARS-CoV-2 immunoglobulins represent a treatment option for COVID-19. However, their production in mammalian cells is not scalable to meet the global demand. Single-domain (VHH) antibodies (also called nanobodies) provide an alternative suitable for microbial production. Using alpaca immune libraries against the receptor-binding domain (RBD) of the SARS-CoV-2 Spike protein, we isolated 45 infection-blocking VHH antibodies. These include nanobodies that can withstand 95°C. The most effective VHH antibody neutralizes SARS-CoV-2 at 17–50 pM concentration (0.2–0.7 µg per liter), binds the open and closed states of the Spike, and shows a tight RBD interaction in the X-ray and cryo-EM structures. The best VHH trimers neutralize even at 40 ng per liter. We constructed nanobody tandems and identified nanobody monomers that tolerate the K417N/T, E484K, N501Y, and L452R immune-escape mutations found in the Alpha, Beta, Gamma, Epsilon, Iota, and Delta/Kappa lineages. We also demonstrate neutralization of the Beta strain at low-picomolar VHH concentrations. We further discovered VHH antibodies that enforce native folding of the RBD in the E. coli cytosol, where its folding normally fails. Such “fold-promoting” nanobodies may allow for simplified production of vaccines and their adaptation to viral escape-mutations

Serum prolactin as a biomarker for the study of intracerebral dopamine effect in adult patients with phenylketonuria: a cross-sectional monocentric study

BACKGROUND: It has been previously postulated that high phenylalanine (Phe) might disturb intracerebral dopamine production, which is the main regulator of prolactin secretion in the pituitary gland. Previously, various associations between Phe and hyperprolactinemia were revealed in studies performed in phenylketonuria (PKU) children and adolescents. The aim of the present study was to clarify whether any relation between serum phenylalanine and prolactin levels can be found in adult PKU patients. PATIENTS AND METHODS: We conducted a cross-sectional, monocentric study including 158 adult patients (male n = 68, female n = 90) with PKU. All patients were diagnosed during newborn screening and were treated since birth. Serum Phe, tyrosine (Tyr), prolactin (PRL), and thyroid-stimulating hormone (TSH) levels were measured, and Phe/Tyr ratio was calculated. Males and females were analyzed separately because the serum prolactin level is gender-dependent. RESULTS: No significant correlations were found between serum phenylalanine, tyrosine, or the Phe/Tyr ratio and serum prolactin level either in the male or in the female group. CONCLUSIONS: In treated adult PKU patients, the serum prolactin level may not be significantly influenced by Phe or Tyr serum levels

Divalent EuRh2Si2 as a reference for the Luttinger theorem and antiferromagnetism in trivalent heavy-fermion YbRh2Si2

Application of the Luttinger theorem to the Kondo lattice YbRh2Si2 suggests that its large 4f-derived Fermi surface (FS) in the paramagnetic (PM) regime should be similar in shape and volume to that of the divalent local-moment antiferromagnet (AFM) EuRh2Si2 in its PM regime. Here we show by angle-resolved photoemission spectroscopy that paramagnetic EuRh2Si2 has a large FS essentially similar to the one seen in YbRh2Si2 down to 1 K. In EuRh2Si2 the onset of AFM order below 24.5 K induces an extensive fragmentation of the FS due to Brillouin zone folding, intersection and resulting hybridization of the Fermi-surface sheets. Our results on EuRh2Si2 indicate that the formation of the AFM state in YbRh2Si2 is very likely also connected with similar changes in the FS, which have to be taken into account in the controversial analysis and discussion of anomalies observed at the quantum critical point in this system

Ladakh: Diverse, high-altitude extreme environments for off-earth analogue and astrobiology research

This paper highlights unique sites in Ladakh, India, investigated during our 2016 multidisciplinary pathfinding expedition to the region. We summarize our scientific findings and the site's potential to support science exploration, testing of new technologies and science protocols within the framework of astrobiology research. Ladakh has several accessible, diverse, pristine and extreme environments at very high altitudes (3000-5700 m above sea level). These sites include glacial passes, sand dunes, hot springs and saline lake shorelines with periglacial features. We report geological observations and environmental characteristics (of astrobiological significance) along with the development of regolith-landform maps for cold high passes. The effects of the diurnal water cycle on salt deliquescence were studied using the ExoMars Mission instrument mockup: HabitAbility: Brines, Irradiance and Temperature (HABIT). It recorded the existence of an interaction between the diurnal water cycle in the atmosphere and salts in the soil (which can serve as habitable liquid water reservoirs). Life detection assays were also tested to establish the best protocols for biomass measurements in brines, periglacial ice-mud and permafrost melt water environments in the Tso-Kar region. This campaign helped confirm the relevance of clays and brines as interest targets of research on Mars for biomarker preservation and life detection.The team would like to express its gratitude to BirbalSahni Institute of Palaeosciences, Department of Science and Technology,Office of Chief Wildlife Warden of Ladakh, Government of India for helpingarrange the requisite clearances and permits for the conducted work. Projectmentoring and guidance provided by Spaceward Bound members at NASAAmes Research Center. Financial and logistics support provided by TataMotors Ltd, Inspired Journeys Co, Pearl Travels Ltd and NationalGeographic Traveller India. Website and IT support provided by the BlueMarble Space Institute of Science. Audio-video documentation support pro-vided by Astroproject India and The H