the spatial ecology off the Guina (Oncifelis guigna) in Southern Chile

This thesis describes the diet, activity, home range and habitat utilisation of guina (Oncifelis guigna) within two populations located inside regions of minimal anthropogenic disturbance in southern Chile. Fieldwork was comprised of several components: guina captured in live traps were fitted with radio collars and monitored on foot using standard radiotelemetry techniques; landcover maps were created for each study area from satellite data, aerial photography and ground truthing, and the relative abundance and diversity of potential small mammal prey were assessed by means of grid-based live-trapping studies and tree-mounted hair-traps. The behavioural data obtained from the radiotelemetry study were analysed using RANGES V to assess the area requirements of the guina and to determine how the home ranges and movements of individuals were distributed with respect to those of conspecifics. This data was also investigated with reference to the landcover maps generated for each site to identify habitat categories that were preferentially utilised or avoided by guina. The diet of this species was determined via faecal analysis. The composition of the guina diet within each site was then related to small mammal relative abundances determined in the field. Small mammals, particularly rodents represented the major component of the guina diet, and no evidence of prey selection was determined. Trapping surveys indicated both sites had abundant prey resources in the form of relatively dense rodent populations. Radiocollared guina were largely arrhythmic in their activity pattern and neighbouring individuals within both populations showed a high degree of spatial overlap, both within and between sexes. Core use areas also overlapped extensively, and no evidence was found to indicate that guina actively avoid conspecifics. These cats exhibited a consistent preference for relatively dense, thicket-forest habitat over less complexly structured vegetation, including stands of Nothofagus forest, the habitat category previously assumed to be key for this species. The results of this study are discussed within the context of conservation management for the continued survival of this endangered felid

Control of crystal polymorph in microfluidics using molluscan 28 kDa Ca2+-binding protein

Biominerals produced by biological systems in physiologically relevant environments possess extraordinary properties that are often difficult to replicate under laboratory conditions. Understanding the mechanism that underlies the process of biomineralisation can lead to novel strategies in the development of advanced materials. Using microfluidics, we have demonstrated for the first time, that an extrapallial (EP) 28 kDa protein, located in the extrapallial compartment between mantle and shell of Mytilus edulis, can influence, at both micro- and nanoscopic levels, the morphology, structure and polymorph that is laid down in the shell ultrastructure. Crucially, this influence is predominantly dependent on the existence of an EP protein concentration gradient and its consecutive interaction with Ca2+ ions. Novel lemon-shaped hollow vaterite structures with a clearly defined nanogranular assembly occur only where particular EP protein and Ca2+ gradients co-exist. Computational fluid dynamics enabled the progress of the reaction to be mapped and the influence of concentration gradients across the device to be calculated. Importantly, these findings could not have been observed using conventional bulk mixing methods. Our findings not only provide direct experimental evidence of the potential influence of EP proteins in crystal formation, but also offer a new biomimetic strategy to develop functional biomaterials for applications such as encapsulation and drug delivery

Structure of myelin P2 protein from equine spinal cord

Equine P2 protein has been isolated from horse spinal cord and its structure determined to 2.1 Å. Since equine myelin is a viable alternative to bovine tissue for large-scale preparations, characterization of the proteins from equine spinal cord myelin has been initiated. There is an unusually high amount of P2 protein in equine CNS myelin compared with other species. The structure was determined by molecular replacement and subsequently refined to an R value of 0.187 (<sub>free</sub> = 0.233). The structure contains a molecule of the detergent LDAO and HEPES buffer in the binding cavity and is otherwise analogous to other cellular retinol-binding proteins

From the stable to the exotic: clustering in light nuclei

A great deal of research work has been undertaken in alpha-clustering study since the pioneering discovery of 12C+12C molecular resonances half a century ago. Our knowledge on physics of nuclear molecules has increased considerably and nuclear clustering remains one of the most fruitful domains of nuclear physics, facing some of the greatest challenges and opportunities in the years ahead. The occurrence of "exotic" shapes in light N=Z alpha-like nuclei is investigated. Various approaches of the superdeformed and hyperdeformed bands associated with quasimolecular resonant structures are presented. Evolution of clustering from stability to the drip-lines is examined: clustering aspects are, in particular, discussed for light exotic nuclei with large neutron excess such as neutron-rich Oxygen isotopes with their complete spectroscopy.Comment: 15 pages, 5 figures, Presented at the International Symposium on "New Horizons in Fundamental Physics - From Neutrons Nuclei via Superheavy Elements and Supercritical Fields to Neutron Stars and Cosmic Rays" held at Makutsi Safari Farm, South Africa, December 23-29, 2015. arXiv admin note: substantial text overlap with arXiv:1402.6590, arXiv:1303.0960, arXiv:1408.0684, arXiv:1011.342

Resistance to Digitisation: Curated Memory Cards Artefact

date-added: 2015-03-24 04:16:59 +0000 date-modified: 2015-03-24 04:16:59 +0000date-added: 2015-03-24 04:16:59 +0000 date-modified: 2015-03-24 04:16:59 +0000The act of networking in any context has some element of ceremonial performance attached to it. In an analogue world these performances have historically included the act of exchanging business cards. This ‘ceremony of networking’ has the potential to be altered by the emergence of new media, especially digital technology, displacing the old ceremony of business card exchanges and disrupting what can traditional be seen as networking. The history of business cards have shown that, despite several digital alternatives, they are still resistant to digitisation and so predominantly still physical and tangible. So, we sought to explore the ceremony around giving business cards as the sharing of ‘curated memory’, to better understand how and why we share and co-create curated memories with others. Including the sharing curated memories more generally, and the changing nature of networking, arising from the ever-increasing connectivity and digital embeddedness associated with the information age. Therefore, exploring the ceremony around needing, creating, sharing and using business cards, within different contexts and cultures. Also, identifying the tasks that people are trying to perform and optimise at different stages (before, during, and after) in a range of scenarios. Also, to explore how the ceremonies of networking might be significantly altered as a result of digital media and tools. The approach of using sets of cards around Who, How, Why and Where emerged from the need for a tool that could build narratives around the considerable diversity of the disjointed scenarios of networking we observed. So, the cards provide a reference by which to share general understanding in an entertaining and easily accessible manner. Second, provides a tool to summarise narratives from the scenarios we observed, and that we could then use to create new scenarios to explore insights such as post-meeting curation of ‘shared memories’ when networking. Third, define a number of ‘games’ to help anyone explore how to better understand and utilise aspects of networking in their current approaches, and challenge them to develop new approaches. Therefore, generating debate and self-reflection on the ways players use business cards themselves

4He decay of excited states in 14C

A study of the 7Li(9Be,4He 10Be)2H reaction at E{beam}=70 MeV has been performed using resonant particle spectroscopy techniques and provides the first measurements of alpha-decaying states in 14C. Excited states are observed at 14.7, 15.5, 16.4, 18.5, 19.8, 20.6, 21.4, 22.4 and 24.0 MeV. The experimental technique was able to resolve decays to the various particle bound states in 10Be, and provides evidence for the preferential decay of the high energy excited states into states in 10Be at ~6 MeV. The decay processes are used to indicate the possible cluster structure of the 14C excited states.Comment: accepted for publication in PR

Ensemble evaluation of hydrological model hypotheses

It is demonstrated for the first time how model parameter, structural and data uncertainties can be accounted for explicitly and simultaneously within the Generalized Likelihood Uncertainty Estimation (GLUE) methodology. As an example application, 72 variants of a single soil moisture accounting store are tested as simplified hypotheses of runoff generation at six experimental grassland field-scale lysimeters through model rejection and a novel diagnostic scheme. The fields, designed as replicates, exhibit different hydrological behaviors which yield different model performances. For fields with low initial discharge levels at the beginning of events, the conceptual stores considered reach their limit of applicability. Conversely, one of the fields yielding more discharge than the others, but having larger data gaps, allows for greater flexibility in the choice of model structures. As a model learning exercise, the study points to a “leaking” of the fields not evident from previous field experiments. It is discussed how understanding observational uncertainties and incorporating these into model diagnostics can help appreciate the scale of model structural error