EVIDENCE OF COMMUNAL OVIPOSITION AND NEST ABANDONMENT IN THE NORTHERN TWO-LINED SALAMANDER (EURYCEA BISLINEATA, (GREEN, 1818)) IN NORTHEASTERN CONNECTICUT

Most plethodontid salamanders oviposit their eggs in an individual nest and attend the clutch until hatching. Here, we describe aspects of the reproduction of Eurycea bislineata (Northern Two-lined Salamander) from three field sites in northeastern Connecticut that contrast with the typical plethodontid reproductive behavior. Rocks used as oviposition sites contained up to 296 eggs, with an average of more than 100. These numbers exceed the maximum ovarian egg counts for this species, indicating that communal oviposition is common. The lack of correlation between rock size and number of eggs, as well as the lack of discrete clutches when eggs are laid in large clusters, suggests that communal oviposition may be caused by something other than nest site limitation. Additionally, the rate of maternal attendance at nests was low. Thus, communal oviposition with high rates of nest abandonment is the dominant reproductive strategy in E. bislineata at these sites

On a Recent Construction of "Vacuum-like" Quantum Field States in Curved Spacetime

Afshordi, Aslanbeigi and Sorkin have recently proposed a construction of a distinguished "S-J state" for scalar field theory in (bounded regions of) general curved spacetimes. We establish rigorously that the proposal is well-defined on globally hyperbolic spacetimes or spacetime regions that can be embedded as relatively compact subsets of other globally hyperbolic spacetimes, and also show that, whenever the proposal is well-defined, it yields a pure quasifree state. However, by explicitly considering portions of ultrastatic spacetimes, we show that the S-J state is not in general a Hadamard state. In the specific case where the Cauchy surface is a round 3-sphere, we prove that the representation induced by the S-J state is generally not unitarily equivalent to that of a Hadamard state, and indeed that the representations induced by S-J states on nested regions of the ultrastatic spacetime also fail to be unitarily equivalent in general. The implications of these results are discussed.Comment: 25pp, LaTeX. v2 References added, typos corrected. To appear in Class Quantum Gravit

Individual differences in loss aversion: conscientiousness predicts how life satisfaction responds to losses versus gains in income

Loss aversion is considered a general pervasive bias occurring regardless of context or person making the decision. We hypothesized that conscientiousness would predict an aversion to losses in the financial domain. We index loss aversion by the relative impact of income losses and gains on life satisfaction. In a representative German sample (N¬ = 105,558: replicated in a British sample, N = 33,848), with conscientiousness measured at baseline, those high on conscientiousness have the strongest reactions to income losses, suggesting a pronounced loss aversion effect, whilst for those moderately un-conscientious there is no loss aversion effect. Our research; (a) provides the first evidence of personality moderation of any loss aversion phenomena; (b) supports contextual perspectives that both personality and situational factors need to be examined in combination; (c) shows that the small but robust relationship with life satisfaction is primarily driven by a subset of people experiencing highly impactful losses

Relating basic properties of bright early-type dwarf galaxies to their location in Abell 901/902

We present a study of the population of bright early-type dwarf galaxies in the multiple-cluster system Abell 901/902. We use data from the STAGES survey and COMBO-17 to investigate the relation between the color and structural properties of the dwarfs and their location in the cluster. The definition of the dwarf sample is based on the central surface brightness and includes galaxies in the luminosity range -16 >= M_B >~-19 mag. Using a fit to the color magnitude relation of the dwarfs, our sample is divided into a red and blue subsample. We find a color-density relation in the projected radial distribution of the dwarf sample: at the same luminosity dwarfs with redder colors are located closer to the cluster centers than their bluer counterparts. Furthermore, the redder dwarfs are on average more compact and rounder than the bluer dwarfs. These findings are consistent with theoretical expectations assuming that bright early-type dwarfs are the remnants of transformed late-type disk galaxies involving processes such as ram pressure stripping and galaxy harassment. This indicates that a considerable fraction of dwarf elliptical galaxies in clusters are the results of transformation processes related to interactions with their host cluster.Comment: 12 pages, 8 figures, accepted for publication in A&A, typo corrected in abstrac

The impact of low erythrocyte density in human blood on the fitness and energetic reserves of the African malaria vector Anopheles gambiae

Background Anaemia is a common health problem in the developing world. This condition is characterized by a reduction in erythrocyte density, primarily from malnutrition and/or infectious diseases such as malaria. As red blood cells are the primary source of protein for haematophagous mosquitoes, any reduction could impede the ability of mosquito vectors to transmit malaria by influencing their fitness or that of the parasites they transmit. The aim of this study was to determine the impact of differences in the density of red blood cells in human blood on malaria vector (Anopheles gambiae sensu stricto) fitness. The hypotheses tested are that mosquito vector energetic reserves and fitness are negatively influenced by reductions in the red cell density of host human blood meals commensurate with those expected from severe anaemia. Methods Mosquitoes (An. gambiae s.s.) were offered blood meals of different packed cell volume(PCV) of human blood consistent with those arising from severe anaemia (15%) and normalPCV (50%). Associations between mosquito energetic reserves (lipid, glucose and glycogen)and fitness measures (reproduction and survival) and blood meal PCV were investigated. Results The amount of protein that malaria vectors acquired from blood feeding (indexed by haematin excretion) was significantly reduced at low blood PCV. However, mosquitoes feeding on blood of low PCV had the same oviposition rates as those feeding on blood of normal PCV, and showed an increase in egg production of around 15%. The long-term survival of An. gambiae s.s was reduced after feeding on low PCV blood, but PCV had no significant impact on the proportion of mosquitoes surviving through the minimal period required to develop and transmit malaria parasites (estimated as 14 days post-blood feeding). The impact of blood PCV on the energetic reserves of mosquitoes was relatively minor. Conclusions These results suggest that feeding on human hosts whose PCV has been depleted due to severe anaemia does not significantly reduce the fitness or transmission potential of malaria vectors, and indicates that mosquitoes may be able exploit resources for reproduction more efficiently from blood of low rather than normal PCV

The Star Formation History in The Far Outer Disc of M33

The outer regions of disc galaxies are becoming increasingly recognized as key testing sites for models of disc assembly and evolution. Important issues are the epoch at which the bulk of the stars in these regions formed and how discs grow radially over time. To address these issues, we use Hubble Space Telescope Advanced Camera for Surveys imaging to study the star formation history (SFH) of two fields at 9.1 and 11.6 kpc along M33's northern major axis. These fields lie at ~ 4 and 5 V-band disc scale-lengths and straddle the break in M33's surface brightness profile. The colour-magnitude diagrams (CMDs) reach the ancient main sequence turnoff with a signal-to-noise ratio of ~ 5. From detailed modelling of the CMDs, we find that the majority of stars in both fields combined formed at z < 1. The mean age in the inner field, S1, is ~ 3 +/- 1 Gyr and the mean metallicity is [M/H] ~ -0.5 +/- 0.2 dex. The star formation history of S1 unambiguously reveals how the inside-out growth previously measured for M33's inner disc out to ~ 6 kpc extends out to the disc edge at ~ 9 kpc. In comparison, the outer field, S2, is older (mean age ~ 7 +/- 2 Gyr), more metal-poor (mean [M/H] ~ -0.8 +/- 0.3 dex), and contains ~ 30 times less stellar mass. These results provide the most compelling evidence yet that M33's age gradient reverses at large radii near the disc break and that this reversal is accompanied by a break in stellar mass surface density. We discuss several possible interpretations of this behaviour including radial stellar mixing, warping of the gaseous disc, a change in star formation efficiency, and a transition to another structural component. These results offer one of the most detailed views yet of the peripheral regions of any disc galaxy and provide a much-needed observational constraint on the last major epoch of star formation in the outer disc.Comment: 15 pages, 9 figures, accepted to MNRAS, hi-res version available at http://www.roe.ac.uk/~mkb/astroph/m33hires.pd

The Discovery of New Galaxy Members in the NGC 5044 and NGC 1052 Groups

We present the results of neutral hydrogen (HI) observations of the NGC 5044 and NGC 1052 groups, as part of a GEMS (Group Evolution Multiwavelength Study) investigation into the formation and evolution of galaxies in nearby groups. Two new group members have been discovered during a wide-field HI imaging survey conducted using the ATNF Parkes telescope. These results, as well as those from followup HI synthesis and optical imaging, are presented here. J1320-1427, a new member of the NGC 5044 Group, has an HI mass of M_HI=1.05e9Msun and M_HI/L_B=1.65 Msun/Lsun, with a radial velocity of v=2750km/s. The optical galaxy is characterised by two regions of star formation, surrounded by an extended, diffuse halo. J0249-0806, the new member of the NGC 1052 Group, has M_HI=5.4e8Msun, M_HI/L_R=1.13 Msun/Lsun and v=1450km/s. The optical image reveals a low surface brightness galaxy. We interpret both of these galaxies as irregular type, with J0249-0806 possibly undergoing first infall into the NGC 1052 group.Comment: Accepted for publication in MNRAS. 21 pages, 13 figures. Also available with high-resolution figures at http://www.astro.livjm.ac.uk/~npfm/Papers/mmb04.ps.g

Synthetic Lethality of Chk1 Inhibition Combined with p53 and/or p21 Loss During a DNA Damage Response in Normal and Tumor Cells

Cell cycle checkpoints ensure genome integrity and are frequently compromised in human cancers. A therapeutic strategy being explored takes advantage of checkpoint defects in p53-deficient tumors in order to sensitize them to DNA-damaging agents by eliminating Chk1-mediated checkpoint responses. Using mouse models, we demonstrated that p21 is a key determinant of how cells respond to the combination of DNA damage and Chk1 inhibition (combination therapy) in normal cells as well as in tumors. Loss of p21 sensitized normal cells to the combination therapy much more than did p53 loss and the enhanced lethality was partially blocked by CDK inhibition. In addition, basal pools of p21 (p53 independent) provided p53 null cells with protection from the combination therapy. Our results uncover a novel p53-independent function for p21 in protecting cells from the lethal effects of DNA damage followed by Chk1 inhibition. As p21 levels are low in a significant fraction of colorectal tumors, they are predicted to be particularly sensitive to the combination therapy. Results reported in this study support this prediction

The fitness of African malaria vectors in the presence and limitation of host behaviour

&lt;p&gt;Background Host responses are important sources of selection upon the host species range of ectoparasites and phytophagous insects. However little is known about the role of host responses in defining the host species range of malaria vectors. This study aimed to estimate the relative importance of host behaviour to the feeding success and fitness of African malaria vectors, and assess its ability to predict their known host species preferences in nature.&lt;/p&gt; &lt;p&gt;Methods Paired evaluations of the feeding success and fitness of African vectors Anopheles arabiensis and Anopheles gambiae s.s in the presence and limitation of host behaviour were conducted in a semi-field system (SFS) at Ifakara Health Institute, Tanzania. In one set of trials, mosquitoes were released within the SFS and allowed to forage overnight on a host that was free to exhibit natural behaviour in response to insect biting. In the other, mosquitoes were allowed to feed directly on from the skin surface of immobile hosts. The feeding success and subsequent fitness of vectors under these conditions were investigated on 6 host types (humans, calves, chickens, cows, dogs and goats) to assess whether physical movements of preferred host species (cattle for An. arabiensis, humans for An. gambiae s.s.) were less effective at preventing mosquito bites than those of common alternatives.&lt;/p&gt; &lt;p&gt;Results Anopheles arabiensis generally had greater feeding success when applied directly to host skin than when foraging on unrestricted hosts (in five of six host species). However, An. gambiae s.s obtained blood meals from free and restrained hosts with similar success from most host types (four out of six). Overall, the blood meal size, oviposition rate, fecundity and post-feeding survival of mosquito vectors were significantly higher after feeding on hosts free to exhibit behaviour, than those who were immobilized during feeding trials.&lt;/p&gt; &lt;p&gt;Conclusions Allowing hosts to move freely during exposure to mosquitoes was associated with moderate reductions in mosquito feeding success, but no detrimental impact to the subsequent fitness of mosquitoes that were able to feed upon them. This suggests that physical defensive behaviours exhibited by common host species including humans do not impose substantial fitness costs on African malaria vectors.&lt;/p&gt