Lispector y Valenzuela: hacia una poética del devenir

Considering the metaliterary focalization as well as the importance of sensible and sensorial traits in the novels El gato eficaz by Luisa Valenzuela and Un soplo de vida by Clarice Lispector this study proposes the realization of a becoming poetics in both literary works, in the sense that Deleuze and Guattari have developed the theory of becoming. Such an aesthetic is based mainly on two significant factors: first, the transformation of the subject in a virtual entity; secondly, the conversion of the discourse into a modality of becoming guided by a proliferation of voices, planes and fictions

Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal

BACKGROUND: Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal. METHODS: 147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing. RESULTS: The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017. CONCLUSIONS: This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal.publishersversionpublishe

On the ordeal of quinolone preparation via cyclisation of aryl-enamines; synthesis and structure of ethyl 6-methyl-7-iodo-4-(3-iodo-4-methylphenoxy)-quinoline-3-carboxylate

Recent studies directed to the design of compounds targeting the bc(1) protein complex of Plasmodium falciparum, the parasite responsible for most lethal cases of malaria, identified quinolones (4-oxo-quinolines) with low nanomolar inhibitory activity against both the enzyme and infected erythrocytes. The 4-oxo-quinoline 3-ester chemotype emerged as a possible source of potent bc(1) inhibitors, prompting us to expand the library of available analogs for SAR studies and subsequent lead optimization. We now report the synthesis and structural characterization of unexpected ethyl 6-methyl-7-iodo-4-(3-iodo-4-methylphenoxy)quinoline-3-carboxylate, a 4-aryloxy-quinoline 3-ester formed during attempted preparation of 6-methyl-7-iodo-4-oxo-quinoline-3-carboxylate (4-oxo-quinoline 3-ester). We propose that the 4-aryloxy-quinoline 3-ester derives from 6-methyl-7-iodo-4-hydroxy-quinoline-3-carboxylate (4-hydroxy-quinoline 3-ester), the enol form of 6-methyl-7-iodo-4-oxo-quinoline-3-carboxylate. Formation of the 4-aryloxy-quinoline 3-ester confirms the impact of quinolone/hydroxyquinoline tautomerism, both on the efficiency of synthetic routes to quinolones and on pharmacologic profiles. Tautomers exhibit different cLogP values and interact differently with the enzyme active site. A structural investigation of 6-methyl-7-iodo-4-oxo-quinoline-3-carboxylate and 6-methyl-7-iodo-4-hydroxy-quinoline-3-carboxylate, using matrix isolation coupled to FTIR spectroscopy and theoretical calculations, revealed that the lowest energy conformers of 6-methyl-7-iodo-4-hydroxy-quinoline-3-carboxylate, lower in energy than their most stable 4-oxo-quinoline tautomer by about 27 kJ mol(-1), are solely present in the matrix, while the most stable 4-oxo-quinoline tautomer is solely present in the crystalline phase.Fundacao para a Ciencia e Tecnologia (FCT - Portugal) [UID/Multi/04326/2013]; QREN-COMPETE-UE; CCMAR; FCT [SFRH/BD/81821/2011, RECI/BBB-BQB/0230/2012, UI0313/QUI/2013, UID/FIS/04564/2016]; FEDER/COMPETE-UE; [PTDC/QEQ-QFI/3284/2014 - POCI-01-0145-FEDER-016617]info:eu-repo/semantics/publishedVersio

Información en línea sobre el transporte intrahospitalario de los pacientes enfermos graves adultos

OBJECTIVE: This study describes the development of an educational web site about intrahospital transportation of critically ill patients. METHODS: The goal of this Web site is to provide information to health care professionals, students, and faculty members about safe transportation of this type of patient. Trochim's model for development of Web sites served as a conceptual framework for this study. RESULTS: The model consists of four general phases: conceptualization, development, implementation, and evaluation. These phases are interrelated to each other and constitute a recurring cycle. CONCLUSIONS: The newly developed web site may serve as a practical guideline to improve the quality of care when transporting critical ill patients. It can be accessed in the World Wide Web at URL: www.unifesp.br/denf/nien/transporte.OBJETIVO: En este artículo se relata el desarrollo de un Web site educativo en relación al transporte intra hospitalario de pacientes críticos adultos. MÉTODOS: Se trata de una investigación aplicada que visa ofrecer informaciones a profesionales de salud, discentes y docentes. El método de desarrollo utilizado, fue el de Trochim. RESULTADOS: comprendió 4 etapas: conceptuación, desarrollo, implementación y la evaluación de forma interactiva y cíclica. CONCLUSIONES: Se sugiere recomendaciones para la práctica clínica basada en evidencias, a fin de mejorar la calidad de la asistencia. Estas informaciones pueden ser buscadas en la World Wide Web, en la URL: www.unifesp.br/denf/nien/transporte .OBJETIVO: Este artigo relata o desenvolvimento de um Web site educacional em transporte intra-hospitalar de pacientes críticos adultos. MÉTODOS: É uma pesquisa aplicada que visa oferecer informações para profissionais de saúde, discentes e docentes. O método de desenvolvimento utilizado, foi o de Trochim. RESULTADOS: compreendeu 4 etapas: conceituação, desenvolvimento, implementação e a avaliação numa forma interativa e cíclica. CONCLUSÕES: Sugere recomendações para a prática clínica baseada em evidências, na busca da melhor qualidade da assistência. Estas informações podem ser acessadas na World Wide Web, na URL: www.unifesp.br/denf/nien/transporte .Centro Universitário Nove de Julho Curso de EnfermagemUniversidade Federal de São Paulo (UNIFESP) Departamento de EnfermagemUNIFESP, Depto. de EnfermagemSciEL

Influence of precipitation and air temperature in production of litterfall in stretch of seasonal forest

A fragmentação e destruição da Mata Atlântica tornam a sua conservação fundamental. A serapilheira é um importante componente desse sistema e apresenta diferenças na deposição nos diferentes ecossistemas brasileiros. Com isso, o objetivo deste estudo foi avaliar o efeito da precipitação e da temperatura do ar no aporte de serapilheira em fragmentos florestais localizados em Sorocaba, SP. A quantidade média de serapilheira aportada foi de 6,9 ± 0,4 mg.ha-1.ano-1, formada por 65% de folhas. Houve diferenças entre as deposições nas estações do ano, sendo que a menor quantidade foi depositada no período seco. A correlação da serapilheira e suas frações não foram significativas quando analisada para o mesmo mês da avaliação da temperatura do ar e precipitação. Porém, quando se relacionam meses anteriores, a correlação é mais expressiva, demonstrando que a resposta da vegetação a variações ambientais não é imediata.Palavras-chave: Clima; sazonalidade; Mata Atlântica. AbstractInfluence of precipitation and air temperature in production of litterfall in stretch of seasonal forest. Fragmentation and destruction of the Atlantic Rainforest make its conservation essential. The litter is an important component of this system, and reveals differences in deposition in the different Brazilian ecosystems. This research aims to evaluate the effect of rainfall and air temperature on litterfall input in a forest fragment at the municipality of Sorocaba, São Paulo State, Brazil. The average contribution of litterfall was 6.9±0.4Mg ha-1 yr-1, comprising 65% of leaves. There were differences between the seasons, with the less amount in the dry season. The correlation of litter and its fractions was not relevant for the same month of air temperature and rainfall measurement. However, the correlation is significant when related to the previous month, demonstrating that the response of vegetation to environmental changes is not immediate.Keywords: Climate; seasonality; Atlantic forest.AbstractFragmentation and destruction of the Atlantic Rainforest make its conservation essential. The litter is an important component of this system, and reveals differences in deposition in the different Brazilian ecosystems. This research aims to evaluate the effect of rainfall and air temperature on litterfall input in a forest fragment at the municipality of Sorocaba, São Paulo State, Brazil. The average contribution of litterfall was 6.9±0.4Mg ha-1 yr-1, comprising 65% of leaves. There were differences between the seasons, with the less amount in the dry season. The correlation of litter and its fractions was not relevant for the same month of air temperature and rainfall measurement. However, the correlation is significant when related to the previous month, demonstrating that the response of vegetation to environmental changes is not immediate.Keywords: Climate; seasonality; Atlantic forest

Heparin Administered to Anopheles in Membrane Feeding Assays Blocks Plasmodium Development in the Mosquito

Innovative antimalarial strategies are urgently needed given the alarming evolution of resistance to every single drug developed against Plasmodium parasites. The sulfated glycosaminoglycan heparin has been delivered in membrane feeding assays together with Plasmodium berghei-infected blood to Anopheles stephensi mosquitoes. The transition between ookinete and oocyst pathogen stages in the mosquito has been studied in vivo through oocyst counting in dissected insect midguts, whereas ookinete interactions with heparin have been followed ex vivo by flow cytometry. Heparin interferes with the parasite's ookinete-oocyst transition by binding ookinetes, but it does not affect fertilization. Hypersulfated heparin is a more efficient blocker of ookinete development than native heparin, significantly reducing the number of oocysts per midgut when offered to mosquitoes at 5 µg/mL in membrane feeding assays. Direct delivery of heparin to mosquitoes might represent a new antimalarial strategy of rapid implementation, since it would not require clinical trials for its immediate deployment

Heparin administered to Anopheles in membrane feeding assays blocks Plasmodium development in the mosquito

Innovative antimalarial strategies are urgently needed given the alarming evolution of resistance to every single drug developed against Plasmodium parasites. The sulfated glycosaminoglycan heparin has been delivered in membrane feeding assays together with Plasmodium berghei-infected blood to Anopheles stephensi mosquitoes. The transition between ookinete and oocyst pathogen stages in the mosquito has been studied in vivo through oocyst counting in dissected insect midguts, whereas ookinete interactions with heparin have been followed ex vivo by flow cytometry. Heparin interferes with the parasite’s ookinete–oocyst transition by binding ookinetes, but it does not affect fertilization. Hypersulfated heparin is a more efficient blocker of ookinete development than native heparin, significantly reducing the number of oocysts per midgut when offered to mosquitoes at 5 µg/mL in membrane feeding assays. Direct delivery of heparin to mosquitoes might represent a new antimalarial strategy of rapid implementation, since it would not require clinical trials for its immediate deployment.publishersversionpublishe

Doença de alzheimer e demandas de cuidados: o que os enfermeiros sabem?

O objetivo deste estudo foi desvelar o conhecimento de enfermeiros de unidades de internação clínica acerca daDoença de Alzheimer (DA) e da demanda de cuidados de pacientes e familiares. Estudo descritivo-exploratório,com abordagem qualitativa. Os dados foram obtidos através de entrevista semi-estruturada. A saturação dos dadosocorreu com 11 enfermeiros de dois hospitais privados. Da análise de conteúdo, emergiram seis categorias: caracterizaçãoda DA, fatores de risco, formas de diagnóstico, complicações, medicações para tratamento, cuidados comdoente e família. Observou-se que os enfermeiros possuem um conhecimento limitado sobre a DA, focando as açõesde cuidado na alteração clínica que motivou a internação hospitalar. Reconhecem sinais e sintomas, mas se mostramcomo figuras coadjuvantes na assistência, quando deveriam posicionar-se de forma mais autônoma frente aocuidado e à atenção à família

Perfil de resultados de exames citopatológicos realizados em uma unidade básica de saúde da família no Município de São José dos Campos - São Paulo / Results profile of cytopathological exams carried out in a basic family health unit in the municipality of São José dos Campos - São Paulo

O câncer de colo uterino é uma afecção progressiva, podendo desenvolver-se, ao longo de uma a duas décadas, para um estágio invasivo. Devido sua evolução lenta, o câncer de colo uterino se diagnosticado precocemente apresenta possibilidade de cura. O objetivo do estudo foi identificar o perfil dos resultados dos exames citológicos do colo do útero em uma Unidade Básica de Saúde da Família. Trata-se de um estudo transversal, analítico e retrospectivo a partir dos resultados de exames citopatológicos realizados durante o primeiro semestre de 2019, por mulheres acompanhadas pela equipe de saúde alocada em uma Unidade de Saúde da Família, localizada na região leste do município de São José dos Campos. Foram levantados e organizados 800 resultados de exames citopatológicos, sendo consolidados em planilhas do Excel 2010 e expresso em gráfico. Os resultados apresentados a partir da porcentagem relativa mostraram uma prevalência (98,62%) de resultados sem alterações ou alterações benignas, além de estarem dentro dos limites de normalidade dos esfregaços citológicos. Foram identificados 0,5% de Lesão de baixo grau (LSIL) e 0,12% de lesão de alto grau (HSIL); presença de infecção por Candida sp. (2,87%) e infecção bacteriana por Gardnerella Vaginalis (13,12%), além do resultado de exame citopatológico com presença do protozoário Trichomonas vaginalis (0,62%). Conclui-se que as prevalências das alterações estão dentro do esperado nas ações do câncer de colo de útero realizado pela equipe da Unidade e atualização dos profissionais responsáveis pela coleta na Unidade permitiu observar a qualidade dos materiais colhidos foram boas. Entretanto mesmo os números de infecções estando dentro do esperado, destaca-se a atenção nas ações de promoção e prevenção às doenças sexualmente transmissíveis.