Tree Composition and Seedling Recruitment in Urban and Rural Forests

In 1993, Dr. Nancy Broshot randomly located 25 permanent study sites in Forest Park in Portland, Oregon to examine the effects of urbanization on forest health. Plant community structure was examined. In 2003, Dr. Broshot reexamined the plant communities at each site and found significantly higher tree mortality and reduced recruitment (young trees) in all areas of the park. Many seedlings that had been present in 1993 were absent in 2003. In 2013, a 20-year follow up study of the tree community was conducted. Although the rate of tree mortality had dropped, recruitment of seedlings and saplings was still low. A series of lichen studies completed at each site in 2013 indicated high levels of nitrogenous air pollution at all sites in the park. In 2014, three control sites along a gradient of air quality in the Mount Hood National Forest above Estacada, Oregon were added to the study. Plant community variables were measured in the same manner as in Forest Park. We found significantly more live trees, saplings and seedlings at the control sites than at sites in Forest Park. We also found significantly fewer dead trees at control sites. Indeed, we had more seedlings at the three control sites than at all 25 of the Forest Park sites. We believe the low level of recruitment may be due to nitrogenous deposition from air pollution in Forest Park; we are waiting for results from collected soil samples to evaluate this hypothesis

Inhibited Synthesis and Erotic Transgression: Georges Bataille in Three Movements

This presentation examines the philosophy of Georges Bataille on three fronts: Part I deals with an examination of Claude Levi-Strauss’ theory on the prohibition of incest, which links the formation of political systems of patrilineal genealogy to the economic reduction of feminine identity at the level of proprietary wealth, defining an exchange fundamental to the movement from nature to society. Bataille disagrees, arguing that the exchange of women is ceremonial, based in giving without expectation of return, claiming that political power is maintained through the sacrifice of wealth. Part II discusses the geopolitical implication of Levi-Strauss’ suggestion that at the opposite end of incest is extreme exogamy, using the example of the South African Apartheid as a case-study in miniature to discuss the “inhibited synthesis” of modern colonial progress in the development of a global order. I critique how the adoption of neoliberal capitalism as a progressive ideology vis-à-vis its promises of national sovereignty and economic partnership fails to recognize the historical entrenchment of political power and wealth accumulation of post-industrial Eurocentric regimes, which systematically exploit new players for their own ends. Bataille assesses this geopolitical deadlock of power by reconsidering the purpose of wealth, arguing that the purpose of wealth is not realized through the capitalist system of profit-investment-accumulation, but rather through acts of useless expenditure, which generate cultural “ecstasies” conducive to synthesis. Part III deals with the critical shift in philosophy and the problem of real access for post-Kantian epistemology, which remains caught in a state of inhibited synthesis between the real and the apparent. Bataille critiques the “failure” of Hegelian idealism to reconstruct the real in the wake of Kant, namely because Hegel still detaches matter, as a unified concept, situating it below the ends of rationality. Bataille argues that matter must be abstracted from conceptual-rational thought, re-constituting thought as matter, and thus allowing for a return of the epistemic access to the real. I argue that Bataille’s work encompasses a major framework that allows for a new kind of speculative realism, envisioning social, geopolitical, and epistemic boundaries

Genome-Wide Association Study of Susceptibility to Idiopathic Pulmonary Fibrosis

Rationale: Idiopathic pulmonary fibrosis (IPF) is a complex lung disease characterised by scarring of the lung that is believed to result from an atypical response to injury of the epithelium. Genome-wide association studies have reported signals of association implicating multiple pathways including host defence, telomere maintenance, signalling and cell-cell adhesion. Objectives: To improve our understanding of factors that increase IPF susceptibility by identifying previously unreported genetic associations. Methods and measurements: We conducted genome-wide analyses across three independent studies and meta-analysed these results to generate the largest genome-wide association study of IPF to date (2,668 IPF cases and 8,591 controls). We performed replication in two independent studies (1,456 IPF cases and 11,874 controls) and functional analyses (including statistical fine-mapping, investigations into gene expression and testing for enrichment of IPF susceptibility signals in regulatory regions) to determine putatively causal genes. Polygenic risk scores were used to assess the collective effect of variants not reported as associated with IPF. Main results: We identified and replicated three new genome-wide significant (P<5×10−8) signals of association with IPF susceptibility (associated with altered gene expression of KIF15, MAD1L1 and DEPTOR) and confirmed associations at 11 previously reported loci. Polygenic risk score analyses showed that the combined effect of many thousands of as-yet unreported IPF susceptibility variants contribute to IPF susceptibility. Conclusions: The observation that decreased DEPTOR expression associates with increased susceptibility to IPF, supports recent studies demonstrating the importance of mTOR signalling in lung fibrosis. New signals of association implicating KIF15 and MAD1L1 suggest a possible role of mitotic spindle-assembly genes in IPF susceptibility

The Transcriptome of Trichuris suis – First Molecular Insights into a Parasite with Curative Properties for Key Immune Diseases of Humans

Iatrogenic infection of humans with Trichuris suis (a parasitic nematode of swine) is being evaluated or promoted as a biological, curative treatment of immune diseases, such as inflammatory bowel disease (IBD) and ulcerative colitis, in humans. Although it is understood that short-term T. suis infection in people with such diseases usually induces a modified Th2-immune response, nothing is known about the molecules in the parasite that induce this response.As a first step toward filling the gaps in our knowledge of the molecular biology of T. suis, we characterised the transcriptome of the adult stage of this nematode employing next-generation sequencing and bioinformatic techniques. A total of ∼65,000,000 reads were generated and assembled into ∼20,000 contiguous sequences ( = contigs); ∼17,000 peptides were predicted and classified based on homology searches, protein motifs and gene ontology and biological pathway mapping.These analyses provided interesting insights into a number of molecular groups, particularly predicted excreted/secreted molecules (n = 1,288), likely to be involved in the parasite-host interactions, and also various molecules (n = 120) linked to chemokine, T-cell receptor and TGF-β signalling as well as leukocyte transendothelial migration and natural killer cell-mediated cytotoxicity, which are likely to be immuno-regulatory or -modulatory in the infected host. This information provides a conceptual framework within which to test the immunobiological basis for the curative effect of T. suis infection in humans against some immune diseases. Importantly, the T. suis transcriptome characterised herein provides a curated resource for detailed studies of the immuno-molecular biology of this parasite, and will underpin future genomic and proteomic explorations

The creolization reader: studies in mixed identities and cultures

This book is not available via ORA

MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network of Regulators of T Helper 2 Cell-Associated Cytokine Production

MicroRNAs (miRNAs) are important regulators of cell fate decisions in immune responses. They act by coordinate repression of multiple target genes, a property that we exploited to uncover regulatory networks that govern T helper-2 (Th2) cells. A functional screen of individual miRNAs in primary T cells uncovered multiple miRNAs that inhibited Th2 cell differentiation. Among these were miR-24 and miR-27, miRNAs coexpressed from two genomic clusters, which each functioned independently to limit interleukin-4 (IL-4) production. Mice lacking both clusters in T cells displayed increased Th2 cell responses and tissue pathology in a mouse model of asthma. Gene expression and pathway analyses placed miR-27 upstream of genes known to regulate Th2 cells. They also identified targets not previously associated with Th2 cell biology which regulated IL-4 production in unbiased functional testing. Thus, elucidating the biological function and target repertoire of miR-24 and miR-27 reveals regulators of Th2 cell biology

Clozapine treatment for suicidality in schizophrenia: International Suicide Prevention Trial (InterSePT)

Background: Approximately 50% of patients with schizophrenia or schizoaffective disorder attempt suicide, and approximately 10% die of suicide. Study results suggest that clozapine therapy significantly reduces suicidal behavior in these patients. Methods: A multicenter, randomized, international, 2-year study comparing the risk for suicidal behavior in patients treated with clozapine vs olanzapine was conducted in 980 patients with schizophrenia or schizoaffective disorder, 26.8% of whom were refractory to previous treatment, who were considered at high risk for suicide because of previous suicide attempts or current suicidal ideation. To equalize clinical contact across treatments, all patients were seen weekly for 6 months and then biweekly for 18 months. Subsequent to randomization, unmasked clinicians at each site could make any interventions necessary to prevent the occurrence of suicide attempts. Suicidal behavior was assessed at each visit. Primary end points included suicide attempts (including those that led to death), hospitalizations to prevent suicide, and a rating of "much worsening of suicidality" from baseline. Masked raters, including an independent suicide monitoring board, determined when end point criteria were achieved. Results: Suicidal behavior was significantly less in patients treated with clozapine vs olanzapine (hazard ratio, 0.76; 95% confidence interval, 0.58-0.97; P = .03). Fewer clozapine-treated patients attempted suicide (34 vs 55; P = .03), required hospitalizations (82 vs 107; P = .05) or rescue interventions (118 vs 155; P = .01) to prevent suicide, or required concomitant treatment with antidepressants (221 vs 258; P = .01) or anxiolytics or soporifics (301 vs 331; P = .03). Overall, few of these high-risk patients died of suicide during the study (5 clozapine vs 3 olanzapine-treated patients; P = .73). Conclusions: Clozapine therapy demonstrated superiority to olanzapine therapy in preventing suicide attempts in patients with schizophrenia and schizoaffective disorder at high risk for suicide. Use of clozapine in this population should lead to a significant reduction in suicidal behavior