Lysine and methionine supplementation in high soy protein content diets for silvery-black porgy (Sparidentex hasta) juveniles

A two-month feeding trial was conducted to evaluate the effect of partial replacement of fish meal (FM) by soybean protein (SP) alone or in combination with lysine (Lys) and methionine (Met) supplementation in practical diets for silvery-black porgy juveniles (16.7±0.1 g). Seven isoproteic (ca. 50% crude protein) and isoenergetic (ca. 22.4 MJ kg-1) diets were formulated in which 45% (SP45), 60% (SP60) and 75% (SP75) of FM protein were replaced by SP and the control diet (FM) was prepared with FM as the major source of protein. In SP45+, SP60+ and SP75+ diets, 45 to 75% of FM was replaced by SP with supplementing blends of Lys and Met. Growth performance, feed utilization, and protein and lipid digestibility decreased with increasing dietary SP levels (p<0.05). Fish fed SP75 had the highest whole body lipid content, but with the lowest whole body arginine, lysine, histidine, phenylalanine and taurine concentrations (p<0.05). Supplementing Lys and Met in SP based diets did not improve growth performance, suggesting that the anti-nutritional factors in soybean protein products rather than Met and Lys deficiency might limit FM substitution with SP in silvery-black porgy juveniles.info:eu-repo/semantics/publishedVersio

Determination of optimum levels of energy and protein on pacific white shrimp (Litopenaeus vannamei) diet at marketing stage

To investigate an economical diet for white pacific prawn (Litopenaeus vannamei) rearing coastal zones of south Iran, 9 experimental diets were prepared considering three protein (37%, 37%, 40% CP) and three digestible energy (262, 312, 362 kcal. 100g^-1 DE) levels, using herbal protein source (soybean meal) as main protein source for the diets. 27 polyethylene round tanks (300 L volume) provided with aerated flow-through water system, stocked with …juvenile shrimps (9.16 ± 0.05 g mean weight) were applied to evaluate the growth performance of the shrimps and feed efficiency parameters of the diets for 8 weeks rearing period in an in-door experimental system. Results showed the diets were significantly different (P≤0.05)in the terms of growth performance and diet efficiency the shrimp, while the best obtaining observed in diet 1 (34% CP and 262 kcal. 100g^-1 DE). Average of weight gain was ranged from 4.58 ± 0.52 g in Diet 9 up to 6.88 ± 0.81 g in diet one, SGR was from 0.73 ± 0.06 in diet 9 to 1.00 ± 0.09 in diet one, survival was from 75.00 ± 7.07 % in diet 9 to 95.00 ± 5.00 % in diet one and FCR was from 5.02 ± 0.94 in diet one to 8.24 ± 1.59 in diet 9. Diets did not effectively influenced the body composition of the shrimp (P>0.05). According to the results of the present study, 34% Cp and 262 kcal. 100g^-1 DE could be suggested as suitable levels to be considered in the diet of the white pacific prawn in the grow-out stage

Designing and establishment of ISO/IEC 17025 in laboratories of national inland water aquaculture center and south Iran aquaculture research center

The project was carried out between June of 2011 and November of 2012,8 laboratories of research center in Anzali (Plankton, Algae, Hydrochemistry, Physiology, Ichthyology, Bentose, Parazitology, Virology) and 7 laboratories of research center in Ahvaz (Clinical pathology, Plankton, Hydrochemistry, Physiology, Ichthyology, Bentose, Parazitology, Virology) were selected for accreditation. The main stages for establishment of the system consisted of: 1-Conducting a gap analysis to compare the present state of the laboratories with ISO/IEC 17025 2-Training General requirements for the competence of testing and calibration laboratories Validation of methods Estimation of uncertainty Internal audits 3- Performing of technical and management requirements 4-Submit of quality manual to ASCB center in England in order to accredit In August of 2012 The main results were including: 1-Increase the accuracy of measurement in laboratories 2-Improvement of the Repeatability and Reproducibility of the test methods 3-Traceability and standardization of test methods 4- Calibration of measurement instruments 6- Updating of test methods 7-Standardization of physical condition of the laboratories 8- Getting the certification from ASCB center i

Legumes Research Volume 2

Hekmatpour, F. et al. - Jiménez-López, José Carlos (CSIC); Clemente, Alfonso (CSIC) Editor