60 research outputs found
Children Under Pressure
Cada vegada els pares pressionen més els fills per por davant un futur que es presenta amb poques possibilitats. La formació, la cultura, segueix semblant una garantia d’èxit. L’estimulació cognitiva ha de començar tan aviat com sigui possible. L’educació ha de ser adquirida al més aviat possible. Cal ensenyar ben aviat calma, ordre i disciplina —principis del món dels adults— perquè després puguin ser competitius. No importa allò que sóc, sinó allò que tinc. La Pràctica Psicomotriu Aucouturier, representada per les escoles de l’ASEFOP (Associació Europea d’Escoles de Formació en Pràctica Psicomotriu), s’oposa a aquesta evolució social: l’objectiu dels nostres esforços no és l’estimulació cognitiva, sinó la maduració psicològica del nen com a resultat d’un desenvolupament personal harmònic. Encara que a Alemanya l’educació preventiva està molt endarrere en relació amb altres països d’Europa, ZAPPA (Zentrum für Aus- und Fortbildung in Psychomotorischer Praxis Aucouturier – Centre de formació i educació en pràctica psicomotriu Aucouturier), escola membre de l’ASEFOP, promou iniciatives d’educació preventiva que mostren com pot ser el futur.Cada día los padres presionan más a sus hijos por temor a un futuro que se presenta con pocas posibilidades. La formación, la cultura, sigue pareciendo una garantía de éxito. La estimulación cognitiva tiene que comenzar cuanto antes. La educación debe ser adquirida tan pronto como sea posible. Hay que enseñar, rápidamente, calma, orden y disciplina —principios del mundo de los adultos— para que después puedan ser competitivos. No importa lo que soy sino lo que tengo. La Práctica Psicomotriz Aucouturier, representada por las escuelas de la ASEFOP (Asociación Europea de Escuelas de Formación en Práctica Psicomotriz), se opone a esta evolución social: el objetivo de nuestros esfuerzos no es la estimulación cognitiva, sino la maduración psicológica del niño, como resultado de un desarrollo personal armónico. Aunque en Alemania la educación preventiva está muy atrasada en comparación con otros países de Europa, ZAPPA (Zentrum für Aus- und Fortbildung in Psychomotorischer Praxis Aucouturier –Centro de formación y educación en práctica psicomotriz Aucouturier), escuela miembro de la ASEFOP, promueve iniciativas de educación preventiva que muestran cómo puede ser el futuro.Every day parents are putting more pressure on their children because of their own fear of a future with little chance. Training, culture, is still a guarantee of success. Cognitive stimulation must begin as soon as possible. Education must be obtained the sooner the better. We must teach quickly, calm, order and discipline —principles of grownups world— so they can then be competitive. It doesn’t matter who I am but what I have. The Psychomotor Practice of Aucouturier, represented by the ASEFOP schools, opposes this social evolution: the aim of our efforts is not cognitive stimulation, but the psychological maturity of the child, as a result of a harmonious personal development. Although preventive education is way behind compared with other European countries in Germany, ZAPPA, school member of the ASEFOP, promotes preventive education initiatives that show how future might be
Cluster of human Puumala orthohantavirus infections due to indoor exposure?—An interdisciplinary outbreak investigation
Puumala orthohantavirus (PUUV) is the most important hantavirus species in Europe, causing the majority of human hantavirus disease cases. In central and western Europe, the occurrence of human infections is mainly driven by bank vole population dynamics influenced by beech mast. In Germany, hantavirus epidemic years are observed in 2- to 5-year intervals. Many of the human infections are recorded in summer and early autumn, coinciding with peaks in bank vole populations. Here, we describe a molecular epidemiological investigation in a small company with eight employees of whom five contracted hantavirus infections in late 2017. Standardized interviews with employees were conducted to assess the circumstances under which the disease cluster occurred, how the employees were exposed and which counteractive measures were taken. Initially, two employees were admitted to hospital and serologically diagnosed with hantavirus infection. Subsequently, further investigations were conducted. By means of a self-administered questionnaire, three additional symptomatic cases could be identified. The hospital patients' sera were investigated and revealed in one patient a partial PUUV L segment sequence, which was identical to PUUV sequences from several bank voles collected in close proximity to company buildings. This investigation highlights the importance of a One Health approach that combines efforts from human and veterinary medicine, ecology and public health to reveal the origin of hantavirus disease clusters.Peer Reviewe
Functionality and Cell Senescence of CD4/ CD8-Selected CD20 CAR T Cells Manufactured Using the Automated CliniMACS Prodigy® Platform
Clinical studies using autologous CAR T cells have achieved
spectacular remissions in refractory CD19+ B cell leukaemia,
however some of the patient treatments with CAR T cells
failed. Beside the heterogeneity of leukaemia, the distribution and senescence of the autologous cells from heavily
pretreated patients might be further reasons for this. We performed six consecutive large-scale manufacturing processes
for CD20 CAR T cells from healthy donor leukapheresis using
the automated CliniMACS Prodigy® platform. Starting with
a CD4/CD8-positive selection, a high purity of a median
of 97% T cells with a median 65-fold cell expansion was
achieved. Interestingly, the transduction rate was significantly higher for CD4+ compared to CD8+ T cells and reached
in a median of 23%. CD20 CAR T cells showed a good specific IFN-γ secretion after cocultivation with CD20+ target
cells which correlated with good cytotoxic activity. Most importantly, 3 out of 5 CAR T cell products showed an increase
in telomere length during the manufacturing process, while
telomere length remained consistent in one and decreased
in another process. In conclusion, this shows for the first time
that beside heterogeneity among healthy donors, CAR T cell
products also differ regarding cell senescence, even for cells
manufactured in a standardised automated process
Estimation of Rift Valley fever virus spillover to humans during the Mayotte 2018–2019 epidemic
Rift Valley fever (RVF) is an emerging, zoonotic, arboviral hemorrhagic fever threatening livestock and humans mainly in Africa. RVF is of global concern, having expanded its geographical range over the last decades. The impact of control measures on epidemic dynamics using empirical data has not been assessed. Here, we fitted a mathematical model to seroprevalence livestock and human RVF case data from the 2018–2019 epidemic in Mayotte to estimate viral transmission among livestock, and spillover from livestock to humans through both direct contact and vector-mediated routes. Model simulations were used to assess the impact of vaccination on reducing the epidemic size. The rate of spillover by direct contact was about twice as high as vector transmission. Assuming 30% of the population were farmers, each transmission route contributed to 45% and 55% of the number of human infections, respectively. Reactive vaccination immunizing 20% of the livestock population reduced the number of human cases by 30%. Vaccinating 1 mo later required using 50% more vaccine doses for a similar reduction. Vaccinating only farmers required 10 times as more vaccine doses for a similar reduction in human cases. Finally, with 52.0% (95% credible interval [CrI] [42.9–59.4]) of livestock immune at the end of the epidemic wave, viral reemergence in the next rainy season (2019–2020) is unlikely. Coordinated human and animal health surveillance, and timely livestock vaccination appear to be key to controlling RVF in this setting. We furthermore demonstrate the value of a One Health quantitative approach to surveillance and control of zoonotic infectious diseases
Risk factors associated with sustained circulation of six zoonotic arboviruses: a systematic review for selection of surveillance sites in non-endemic areas
Arboviruses represent a signifcant burden to public health and local economies due to their ability to cause unpredictable and widespread epidemics. To maximize early detection of arbovirus emergence in non-endemic areas,
surveillance eforts should target areas where circulation is most likely. However, identifying such hotspots of potential emergence is a major challenge. The ecological conditions leading to arbovirus outbreaks are shaped by complex
interactions between the virus, its vertebrate hosts, arthropod vector, and abiotic environment that are often poorly
understood. Here, we systematically review the ecological risk factors associated with the circulation of six arboviruses
that are of considerable concern to northwestern Europe. These include three mosquito-borne viruses (Japanese
encephalitis virus, West Nile virus, Rift Valley fever virus) and three tick-borne viruses (Crimean-Congo hemorrhagic
fever virus, tick-borne encephalitis virus, and louping-ill virus). We consider both intrinsic (e.g. vector and reservoir
host competence) and extrinsic (e.g. temperature, precipitation, host densities, land use) risk factors, identify current
knowledge gaps, and discuss future directions. Our systematic review provides baseline information for the identifcation of regions and habitats that have suitable ecological conditions for endemic circulation, and therefore may be
used to target early warning surveillance programs aimed at detecting multi-virus and/or arbovirus emergence
Whole Grain Wheat Consumption Affects Postprandial Inflammatory Response in a Randomized Controlled Trial in Overweight and Obese Adults with Mild Hypercholesterolemia in the Graandioos Study
BACKGROUND: Whole grain wheat (WGW) consumption is associated with health benefits in observational studies. However, WGW randomized controlled trial (RCT) studies show mixed effects. OBJECTIVES: The health impact of WGW consumption was investigated by quantification of the body's resilience, which was defined as the "ability to adapt to a standardized challenge." METHODS: A double-blind RCT was performed with overweight and obese (BMI: 25-35 kg/m2) men (n = 19) and postmenopausal women (n = 31) aged 45-70 y, with mildly elevated plasma total cholesterol (>5 mmol/L), who were randomly assigned to either 12-wk WGW (98 g/d) or refined wheat (RW). Before and after the intervention a standardized mixed-meal challenge was performed. Plasma samples were taken after overnight fasting and postprandially (30, 60, 120, and 240 min). Thirty-one biomarkers were quantified focusing on metabolism, liver, cardiovascular health, and inflammation. Linear mixed-models evaluated fasting compared with postprandial intervention effects. Health space models were used to evaluate intervention effects as composite markers representing resilience of inflammation, liver, and metabolism. RESULTS: Postprandial biomarker changes related to liver showed decreased alanine aminotransferase by WGW (P = 0.03) and increased β-hydroxybutyrate (P = 0.001) response in RW. Postprandial changes related to inflammation showed increased C-reactive protein (P = 0.001), IL-6 (P = 0.02), IL-8 (P = 0.007), and decreased IL-1B (P = 0.0002) in RW and decreased C-reactive protein (P < 0.0001), serum amyloid A (P < 0.0001), IL-8 (P = 0.02), and IL-10 (P < 0.0001) in WGW. Health space visualization demonstrated diminished inflammatory (P < 0.01) and liver resilience (P < 0.01) by RW, whereas liver resilience was rejuvenated by WGW (P < 0.05). CONCLUSIONS: Twelve-week 98 g/d WGW consumption can promote liver and inflammatory resilience in overweight and obese subjects with mildly elevated plasma cholesterol. The health space approach appeared appropriate to evaluate intervention effects as composite markers. This trial was registered at www.clinicaltrials.gov as NCT02385149.</p
A Fly in the Ointment: Evaluation of Traditional Use of Plants to Repel and Kill Blowfly Larvae in Fermented Fish
Introduction: In rural areas in Laos, fly larvae infestations are common in fermenting fish. Blowflies (Chrysomyamegacephala, Diptera: Calliphoridae) are attracted to oviposit (and/or larviposit) onto fermenting fish which results ininfestations with fly larvae. Knowledge of traditional use of plants to repel larvae during the production of fermented fish iscommon and widespread in Lao PDR. Research Questions: How effective are the most salient species in repelling, and killing fly larvae in fermenting fish? Material and Methods: The three plant species most frequently reported to repel fly larvae during an ethnobotanical surveythroughout Lao PDR were tested for repellence and larvicidal activity of fly larvae infesting fermented fish. The lethality andrepellence of Tadehagi triquetrum (L.) H. Ohashi (Fabaceae), Uraria crinita (L.) Desv. ex DC. (Fabaceae) and Bambusa multiplex(Lour.) Raeusch. ex Schult. & Schult. f. (Poaceae) were tested in an experimental design using fermenting fish in Vientiane,Lao PDR. Results: The repellent effect of fresh material of T. triquetrum and U. crinita, and the larvicidal effect of fresh B. multiplex, issignificantly more effective than that of dried material of the same species, and the total effect (repellence and larvicidaleffect combined) for each of the three species was significantly more effective for fresh than for dry material. Fresh materialof T. triquetrum, U. crinita, or B. multiplex added on top of the fermenting fish repelled 50%, 54%, 37%, and killed 22%, 28%,and 40% of fly larvae. The total effect was not significantly different per species at 72%, 82%, and 77%, respectively. Discussion and Conclusions: The three most salient species are effective in repelling and killing fly larvae in the productionof fermented fish, and may be essential to augment food safety during traditional fermentation in open jars
Twelve-month effectiveness and safety of bictegravir/emtricitabine/tenofovir alafenamide in people with HIV: Real-world insights from BICSTaR cohorts
Background: Real-world evidence is an essential component of evidence-based medicine. The aim of the BICSTaR (BICtegravir Single Tablet Regimen) study is to assess effectiveness and safety of bictegravir/emtricitabine/tenofovir alafenamide (B/F/TAF) in antiretroviral treatment-naïve (TN) and treatment-experienced (TE) people with HIV. Methods: BICSTaR is a prospective, observational cohort study. Participants (≥18 years) are being followed for 24 months. A pooled analysis is presented at 12 months, with the primary endpoint of effectiveness (HIV-1 RNA <50 copies/mL) and secondary endpoints of safety and tolerability (as per protocol). An exploration of patient-reported outcome measures using standardized questionnaires is included. Results: Between June 2018 and May 2021, 1552 people with HIV were enrolled across 12 countries. The analysed population comprised 1509 individuals (279 TN, 1230 TE); most were white (76%), male (84%) and had one or more comorbid conditions (68%). Median age was 47 years. After 12 months of B/F/TAF treatment, HIV-1 RNA was <50 copies/mL in 94% (221/236) of TN participants and 97% (977/1008) of TE participants. Median CD4 cell count increased by 214 cells/μL (p < 0.001) in TN participants and 13 cells/μL (p = 0.014) in TE participants; median CD4/CD8 ratios increased by 0.30 and 0.03, respectively (both p < 0.001). Persistence was high at 12 months (TN, 97%; TE, 95%). No resistance to B/F/TAF emerged. Study drug-related adverse events occurred in 13% of participants through 12 months, leading to B/F/TAF discontinuation in 6%. Conclusions: The findings of this study provide robust real-world evidence to support the broad use of B/F/TAF in both TN and TE people with HIV
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