Population gene introgression and high genome plasticity for the zoonotic pathogen Streptococcus agalactiae

The influence that bacterial adaptation (or niche partitioning) within species has on gene spillover and transmission among bacteria populations occupying different niches is not well understood. Streptococcus agalactiae is an important bacterial pathogen that has a taxonomically diverse host range making it an excellent model system to study these processes. Here we analyze a global set of 901 genome sequences from nine diverse host species to advance our understanding of these processes. Bayesian clustering analysis delineated twelve major populations that closely aligned with niches. Comparative genomics revealed extensive gene gain/loss among populations and a large pan-genome of 9,527 genes, which remained open and was strongly partitioned among niches. As a result, the biochemical characteristics of eleven populations were highly distinctive (significantly enriched). Positive selection was detected and biochemical characteristics of the dispensable genes under selection were enriched in ten populations. Despite the strong gene partitioning, phylogenomics detected gene spillover. In particular, tetracycline resistance (which likely evolved in the human-associated population) from humans to bovine, canines, seals, and fish, demonstrating how a gene selected in one host can ultimately be transmitted into another, and biased transmission from humans to bovines was confirmed with a Bayesian migration analysis. Our findings show high bacterial genome plasticity acting in balance with selection pressure from distinct functional requirements of niches that is associated with an extensive and highly partitioned dispensable genome, likely facilitating continued and expansive adaptation

Reimagining Wilmer's Park

Final project for LARC:340 Site Planning and Design Studio (Fall 2022). University of Maryland, College Park.Wilmer’s Park is a “80-acre parcel containing the ruins of a dance hall, motel, ranch house, covered stage, baseball and football fields. As a major stop on the Chitlin Circuit, Wilmer’s Park opened its doors to African-American musicians, entertainers, athletes and fans from the early 1950s through the late 1960s. Arthur Wilmer used his experience and connections developed as the owner of a night club in Washington, D. C. to bring both popular acts and up-and-coming performers to rural Prince George’s County; the bandstand at Wilmer’s Park showcased everyone from Duke Ellington and Otis Redding to the Temptations, Patti La Belle, and a young Stevie Wonder. The former tobacco farm played an important role in exposing emerging musicians to local African Americans during a time of segregation.” The park has been closed for 10+ years and the purpose of this project is to transform Wilmer’s Park for the residents of Brandywine or nearby communities. For this project, students work in teams of three to design a master plan along with an individual detailed site plan. The design program for these plans came from the residents’ comments from community engagement workshops, notes from Councilman Harrison’s interview, important stakeholders, the field trip, and guest lectures. The master plan does not include all 80 acres of the park and often identifies a phasing plan for the entire project.Prince George's County Parks Departmen

Factors Associated with Revision Surgery after Internal Fixation of Hip Fractures

Background: Femoral neck fractures are associated with high rates of revision surgery after management with internal fixation. Using data from the Fixation using Alternative Implants for the Treatment of Hip fractures (FAITH) trial evaluating methods of internal fixation in patients with femoral neck fractures, we investigated associations between baseline and surgical factors and the need for revision surgery to promote healing, relieve pain, treat infection or improve function over 24 months postsurgery. Additionally, we investigated factors associated with (1) hardware removal and (2) implant exchange from cancellous screws (CS) or sliding hip screw (SHS) to total hip arthroplasty, hemiarthroplasty, or another internal fixation device. Methods: We identified 15 potential factors a priori that may be associated with revision surgery, 7 with hardware removal, and 14 with implant exchange. We used multivariable Cox proportional hazards analyses in our investigation. Results: Factors associated with increased risk of revision surgery included: female sex, [hazard ratio (HR) 1.79, 95% confidence interval (CI) 1.25-2.50; P = 0.001], higher body mass index (fo

Examining the Differences of External Causes of Death in Black, Indigenous, and People of Color versus the Non-Hispanic White Population of Vermont

Objectives: Our objective in this study was to examine differences in risk of death from various external causes between the BIPOC community and individuals who identified as NHW. Methods: 2015-2019 death certificate data accessible on the Vermont Electronic Data Registration System (EDRS) was analyzed and categorized by external causes of death. Decedent race/ethnicity was assigned as BIPOC or NHW according to the race/ethnicity listed on EDRS. Mortality rates were calculated using binary logistic regression and variable significance was calculated using multivariable logistic regression. Results: There were varied external causes of death between BIPOC and NHW populations. The risk of external causes of death were 65.3% lower among BIPOC individuals who were married. The odds of death by external causes are 52.6% greater for individuals who did not graduate high school. Assault deaths constituted the largest proportion of BIPOC external causes of death. Discussion: There was an increase in certain external causes of death affecting BIPOC Vermonters, even in a state of racial and ethnic homogeneity. This study’s results imply a need for increased health equity reform and the continued expansion of the Vermont Race Equity Task Force

Low-Intensity Walking Activity Is Associated With Better Health

Recommended levels of physical activity may represent challenging targets for many older adults at risk for disability, leading to the importance of evaluating whether low-intensity activity is associated with health benefits. We examined the cross-sectional association between low-intensity walking activity (<100 steps/min) and health and physical function in a group of older adults. Participants (N = 187; age = 66.8; 91.4% African American; 76.5% female) wore a StepWatch Activity Monitor to measure components of low-intensity walking activity. Only 7% of participants met physical activity guidelines and moderate-intensity activity (≥100 steps/min) contributed only 10% of the total steps/day and 2% of the total min/day. Greater amount, frequency, and duration of low-intensity activity were associated with better self-report and performance-based measures of physical function, better quality of life, and fewer depressive symptoms (ps < .05). The cross-sectional relationship between low-intensity activity and health outcomes important to independent function suggests that we further explore the longitudinal benefits of low-intensity activity

Dynamic capabilities in a sixth generation family firm: entrepreneurship and the Bibby Line

In this paper we draw on the theory of dynamic capabilities to examine development of the only surviving family-owned Liverpool shipping company. The Bibby Line was founded in 1807 to take advantage of the growing sea-trade based in Liverpool. The company remained in shipping until the mid-1960s, when a series of external crises led the owner, Derek Bibby, to begin a process of diversification. In the last 50 years, the Bibby Line has grown into a £1bn business with interests in retail, distribution and financial services as well as a continuing commitment to shipping. Our intention is to demonstrate how multigenerational ownership contributes to the creation of dynamic capabilities in family firms. The distinctive nature of Bibby as a long-standing family business is related to unique assets such as patient capital, flexible governance structures as well as the ability to mobilise social and human capital