A Search for Parent-of-Origin Effects on Honey Bee Gene Expression

Parent-specific gene expression (PSGE) is little known outside of mammals and plants. PSGE occurs when the expression level of a gene depends on whether an allele was inherited from the mother or the father. Kin selection theory predicts that there should be extensive PSGE in social insects because social insect parents can gain inclusive fitness benefits by silencing parental alleles in female offspring. We searched for evidence of PSGE in honey bees using transcriptomes from reciprocal crosses between European and Africanized strains. We found 46 transcripts with significant parent-of-origin effects on gene expression, many of which overexpressed the maternal allele. Interestingly, we also found a large proportion of genes showing a bias toward maternal alleles in only one of the reciprocal crosses. These results indicate that PSGE may occur in social insects. The nonreciprocal effects could be largely driven by hybrid incompatibility between these strains. Future work will help to determine if these are indeed parent-of-origin effects that can modulate inclusive fitness benefits

New approach to detect seismic surface waves in 1Hz-sampled GPS time series

Recently, co-seismic seismic source characterization based on GPS measurements has been completed in near- and far-field with remarkable results. However, the accuracy of the ground displacement measurement inferred from GPS phase residuals is still depending of the distribution of satellites in the sky. We test here a method, based on the double difference (DD) computations of Line of Sight (LOS), that allows detecting 3D co-seismic ground shaking. The DD method is a quasi-analytically free of most of intrinsic errors affecting GPS measurements. The seismic waves presented in this study produced DD amplitudes 4 and 7 times stronger than the background noise. The method is benchmarked using the GEONET GPS stations recording the Hokkaido Earthquake (2003 September 25th, Mw = 8.3)

Long-period surface motion of the multi-patch Mw9.0 Tohoku-Oki earthquake

We show that it is possible to capture the oscillatory ground motion induced by the Tohoku-Oki event for periods ranging from 3 to 100s using Precise Point Positioning (PPP). We find that the ground motions of the sedimentary basins of Japan were large (respectively > 0.15m/s and >0.15m/s2 for velocity and acceleration) even for periods larger than 3s. We compare geodetic observables with a Ground Motion Prediction Equation (GMPE) designed for Japan seismicity and find that the Spectral Acceleration (SA) is well estimated for periods larger than 3s and distances ranging from 100 to 500km. At last, through the analysis of the displacement attenuation plots, we show that the 2011 Tohoku-Oki event is likely composed of multiple rupture patches as suggested before by time-reversal inversions of seismic data

The Making of a Queen: TOR Pathway Is a Key Player in Diphenic Caste Development

Honey bees (Apis mellifera) provide a principal example of diphenic development. Excess feeding of female larvae results in queens (large reproductives). Moderate diet yields workers (small helpers). The signaling pathway that links provisioning to female developmental fate is not understood, yet we reasoned that it could include TOR (target of rapamycin), a nutrient- and energy-sensing kinase that controls organismal growth.Here, the role of Apis mellifera TOR (amTOR) in caste determination is examined by rapamycin/FK506 pharmacology and RNA interference (RNAi) gene knockdown. We show that in queen-destined larvae, the TOR inhibitor rapamycin induces the development of worker characters that are blocked by the antagonist FK506. Further, queen fate is associated with elevated activity of the Apis mellifera TOR encoding gene, amTOR, and amTOR gene knockdown blocks queen fate and results in individuals with worker morphology.A much-studied insect dimorphism, thereby, can be governed by the TOR pathway. Our results present the first evidence for a role of TOR in diphenic development, and suggest that adoption of this ancestral nutrient-sensing cascade is one evolutionary pathway for morphological caste differentiation in social insects

Behavioral genomics of honeybee foraging and nest defense

The honeybee has been the most important insect species for study of social behavior. The recently released draft genomic sequence for the bee will accelerate honeybee behavioral genetics. Although we lack sufficient tools to manipulate this genome easily, quantitative trait loci (QTLs) that influence natural variation in behavior have been identified and tested for their effects on correlated behavioral traits. We review what is known about the genetics and physiology of two behavioral traits in honeybees, foraging specialization (pollen versus nectar), and defensive behavior, and present evidence that map-based cloning of genes is more feasible in the bee than in other metazoans. We also present bioinformatic analyses of candidate genes within QTL confidence intervals (CIs). The high recombination rate of the bee made it possible to narrow the search to regions containing only 17–61 predicted peptides for each QTL, although CIs covered large genetic distances. Knowledge of correlated behavioral traits, comparative bioinformatics, and expression assays facilitated evaluation of candidate genes. An overrepresentation of genes involved in ovarian development and insulin-like signaling components within pollen foraging QTL regions suggests that an ancestral reproductive gene network was co-opted during the evolution of foraging specialization. The major QTL influencing defensive/aggressive behavior contains orthologs of genes involved in central nervous system activity and neurogenesis. Candidates at the other two defensive-behavior QTLs include modulators of sensory signaling (Am5HT(7) serotonin receptor, AmArr4 arrestin, and GABA-B-R1 receptor). These studies are the first step in linking natural variation in honeybee social behavior to the identification of underlying genes

Denial of Motherhood: An Analysis on Government Regulation of Black Women's Sexuality and Reproduction

The purpose of this senior thesis is to examine the implicit racial underpinnings of government polices that work to regulate black women’s reproduction. Drawing connections to past racially influenced government polices, the study demonstrates how black women’s reproductive autonomy has been a frequent target of large-scale government intrusion and manipulation for centuries. Based on this historical precedent, this research aims to show that current government policies operate in a similar way by targeting black women’s reproductive behavior through implicit racism. Using family cap provisions of state welfare programs as a lens through which to analyze this assertion, this study seeks to demonstrate how such family cap provisions work as a contemporary mechanism of institutionalized racism that acts as a continuum of the historical racial bias against black women’s reproductive autonomy

US NBA Influence on French Basketball: investigating the impact of the NBA marketing techniques on French basketball clubs as Perceived by their Professionals and Fans.

The National Basketball Association (NBA) is one of the most powerful sport leagues in the world. Understanding NBA marketing strategy is the point of departure of many sport leagues or clubs willing to become more powerful. The influence of the NBA in today’s basketball world is extremely significant. The French basketball league, named Ligue Nationale de Basketball (LNB), is one example of NBA influence. The purpose of this study is to investigate this impact of NBA internationalization on the French Basketball clubs, through the prism of marketing techniques. The author applied an inductive approach and interview as research method by interviewing 5 participants (three fans and two marketers), in order to collect both quantitative and qualitative data. The results confirmed the initial consideration that there is a significant impact of the NBA on the LNB, especially in terms of adoption of similar marketing techniques including social media, attractive content around the stars and the pre and post-match shows or event, but also promotional giveaways and outdoor advertising. Moreover, results highlighted a category of NBA marketing techniques applied in French basketball but not effective in the French market such as E-mail offers, television, but aslo mini packs, group sales, and booster memberships. Finally, the results also show that a part of the NBA marketing techniques used in the American basketball league were adapted yet not successful on the French basketball market such as virtual reality marketing, direct mails, employee incentives nights, face to face meetings with businesses, grass roots marketing, referrals, interactive sales center experiences, radio advertising and telemarketing. Future research could benefit from larger more representative sample as well as a comparative study of NBA impact on other national leagues across the world