Fixed drug eruption induced by atenolol

Fixed drug eruption (FDE) is characterized by recurrent well-defined lesions in the same location each time the responsible drug is taken. We report here a case of multiple FDE induced by atenolol in a 48-year-old woman confirmed by positive patch test in previously affected sites. Beta-blockers-induced FDE are very rare. Only two cases had been reported in the literature. To the best of our knowledge, this is the first case reported of atenolol-induced FDE confirmed by a positive patch test

PENGARUH PENERAPAN MODEL DISCOVERY LEARNING BERBASIS TPACK (Technological, Pedagogical, Content, Knowledge) TERHADAP KEMAMPUAN BERPIKIR TINGKAT TINGGI (HOTS) SISWA

Penelitian ini dilatar belakangi oleh kemampuan berpikir tingkat tinggi (HOTS) pada siswa yang masih rendah. Higher Order Thinking Skills (HOTS) merupakan bagian dari tuntutan kurikulum 2013. Model Discovery Learning berbasis TPACK merupakan salah satu model yang sesuai dengan penerapan kurikulum 2013. Penelitian ini bertujuan untuk mengetahui pengaruh penerapan model discovery learning berbasis TPACK (Technological, Pedagogical, Content, Knowledge) terhadap kemampuan berpikir tingkat tinggi (HOTS) siswa kelas XI MIPA di SMA Negeri 1 Sariwangi pada sub materi sistem pernapasan manusia. Subyek penelitian ini adalah siswa kelas XI MIPA 2 yang berjumlah 30 orang. Pengambilan sampel dilakukan dengan teknik purposive sampling. Desain penelitian menggunakan one group pretest posttest desain. Instrumen penelitian berupa soal tes kemampuan berpikir tingkat tinggi yang meliputi analisis, evaluasi dan mencipta dalam bentuk uraian/essay. Data hasil penelitian dianalisis secara statistik menggunakan uji N-Gain, uji normalitas dan uji hipotesis dengan menggunakan uji Z. Hasil penelitian menunjukkan peningkatan kemampuan berpikir tingkat tinggi siswa dinilai tinggi dengan rata-rata 0,82, data berdistribusi normal dan hipotesis diterima. Maka dapat disimpulkan bahwa terdapat pengaruh yang sangat signifikan penerapan model discovery learning berbasis TPACK (Technological, Pedagogical, Content, Knowledge) terhadap kemampuan berpikir tingkat tinggi (HOTS) siswa pada sub materi system pernapasan manusia.Kata Kunci: Higher Order Thinking Skills (HOTS). Model Discovery Learning, TPACK

Reconstructing terrestrial nutrient cycling using stable nitrogen isotopes in wood

Although recent anthropogenic effects on the global nitrogen (N) cycle have been significant, the consequences of increased anthropogenic N on terrestrial ecosystems are unclear. Studies of the impact of increased reactive N on forest ecosystems—impacts on hydrologic and gaseous loss pathways, retention capacity, and even net primary productivity— have been particularly limited by a lack of long-term baseline biogeochemical data. Stable nitrogen isotope analysis (ratio of ¹⁵N to ¹⁴N, termed δ¹⁵N) of wood chronologies offers the potential to address changes in ecosystem N cycling on millennial timescales and across broad geographic regions. Currently, nearly 50 studies have been published utilizing wood δ¹⁵N records; however, there are significant differences in study design and data interpretation. Here, we identify four categories of wood δ¹⁵N studies, summarize the common themes and primary findings of each category, identify gaps in the spatial and temporal scope of current wood δ¹⁵N chronologies, and synthesize methodological frameworks for future research by presenting eight suggestions for common methodological approaches and enhanced integration across studies. Wood δ¹⁵N records have the potential to provide valuable information for interpreting modern biogeochemical cycling. This review serves to advance the utility of this technique for long-term biogeochemical reconstructions

A degenerate primer MOB typing (DPMT) method to classify gamma-proteobacterial plasmids in clinical and environmental settings

Transmissible plasmids are responsible for the spread of genetic determinants, such as antibiotic resistance or virulence traits, causing a large ecological and epidemiological impact. Transmissible plasmids, either conjugative or mobilizable, have in common the presence of a relaxase gene. Relaxases were previously classified in six protein families according to their phylogeny. Degenerate primers hybridizing to coding sequences of conserved amino acid motifs were designed to amplify related relaxase genes from γ-Proteobacterial plasmids. Specificity and sensitivity of a selected set of 19 primer pairs were first tested using a collection of 33 reference relaxases, representing the diversity of γ-Proteobacterial plasmids. The validated set was then applied to the analysis of two plasmid collections obtained from clinical isolates. The relaxase screening method, which we call "Degenerate Primer MOB Typing" or DPMT, detected not only most known Inc/Rep groups, but also a plethora of plasmids not previously assigned to any Inc group or Rep-type

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

Microbial Biotechnolog