Plant diversity effects on plant longevity and their relationships to population stability in experimental grasslands

Identifying to what degree inherent characteristics of plant species and their variation in response to their environment regulate the temporal stability of plant populations is important to understand patterns of species coexistence and the stability of ecosystems. Longevity is a key characteristic of plant life history and an important component of demographic storage, but age is usually unknown for herbaceous species. In a 12-year-old biodiversity experiment (Jena Experiment) comprising 80 grassland communities with six levels of plant species richness (1, 2, 4, 8, 16 and 60 species) and four levels of functional groups richness (1, 2, 3 and 4 functional groups), we studied populations of 38 dicotyledonous forb species (N = 1,683 plant individuals). The sampled individuals represented three plant functional groups (legumes, small herbs and tall herbs) and two different growth forms (species with long-lived primary roots and clonal species with rhizomes/stolons). We assessed the age of plant individuals by means of growth ring analysis and related the age of plant populations to their temporal stability in terms of peak biomass production. On average, plant species richness did not affect the mean age of the populations or the maximum age of individuals found in a population. Age of herbs with taproots increased and age of herbs with clonal growth decreased with increasing species richness, cancelling out each other when growth forms were analysed together. Mean population age was lowest for small herbs and highest for tall herbs, while legumes had an intermediate population age. Herbs with a taproot were on average older than herbs with a rhizome. Across all species-richness levels, populations with older individuals were more stable in terms of biomass production over time. Synthesis. Our study shows for the first time across multiple species that the longevity of forbs is affected by the diversity of the surrounding plant community, and that plant longevity as an important component of demographic storage increases the temporal stability of populations of grassland forb species

Mid-calf skeletal muscle density and its associations with physical activity, bone health and incident 12-month falls in older adults: The Healthy Ageing Initiative

Accepted manuscript version, licensed CC BY-NC-ND 4.0. Background - Lower skeletal muscle density, indicating greater infiltration of adipose tissue into muscles, is associated with higher fracture risk in older adults. We aimed to determine whether mid-calf muscle density is associated with falls risk and bone health in community-dwelling older adults. Methods - 2214 community-dwelling men and women who participated in the Healthy Ageing Initiative (Sweden) study at age 70 were included in this analysis. Mid-calf muscle density (mg/cm3) at the proximal tibia, and volumetric bone mineral density (vBMD) and architecture at the distal and proximal tibia and radius, were assessed by peripheral quantitative computed tomography. Whole-body lean and fat mass, lumbar spine and total hip areal bone mineral density (aBMD) were assessed by dual-energy X-ray absorptiometry. Participants completed seven-day accelerometer measurements of physical activity intensity, and self-reported falls data were collected 6 and 12 months later. Results - 302 (13.5%) participants reported a fall at the 6- or 12-month interview, and 29 (1.3%) reported a fall at both interviews. After adjustment for confounders, each standard deviation decrease in mid-calf muscle density was associated with a trend towards greater likelihood of experiencing a fall (OR 1.13; 95% CI 1.00, 1.29 per SD lower) and significantly greater likelihood of multiple falls (1.61; 1.16, 2.23). Higher muscle density was not associated with total hip aBMD, and was associated with lower lumbar spine aBMD (B = -0.003; 95% CI -0.005, -0.001 per mg/cm3) and higher proximal cortical vBMD (0.74; 0.20, 1.28) at the radius. At the tibia, muscle density was positively associated with distal total and trabecular vBMD, and proximal total and cortical vBMD, cortical thickness, cortical area and stress-strain index (all P  Conclusions - Lower mid-calf muscle density is independently associated with higher likelihood for multiple incident falls and appears to have localised negative effects on bone structure in older adults

Astrophysical Tests of Modified Gravity: A Screening Map of the Nearby Universe

Astrophysical tests of modified modified gravity theories in the nearby universe have been emphasized recently by Hui, Nicolis and Stubbs (2009) and Jain and VanderPlas (2011). A key element of such tests is the screening mechanism whereby general relativity is restored in massive halos or high density environments like the Milky Way. In chameleon theories of gravity, including all f(R) models, field dwarf galaxies may be unscreened and therefore feel an extra force, as opposed to screened galaxies. The first step to study differences between screened and unscreened galaxies is to create a 3D screening map. We use N-body simulations to test and calibrate simple approximations to determine the level of screening in galaxy catalogs. Sources of systematic errors in the screening map due to observational inaccuracies are modeled and their contamination is estimated. We then apply our methods to create a map out to 200 Mpc in the Sloan Digital Sky Survey footprint using data from the Sloan survey and other sources. In two companion papers this map will be used to carry out new tests of gravity using distance indicators and the disks of dwarf galaxies. We also make our screening map publicly available.Comment: 21 pages, 10 figure

Functional analysis and transcriptional output of the Göttingen minipig genome

In the past decade the Göttingen minipig has gained increasing recognition as animal model in pharmaceutical and safety research because it recapitulates many aspects of human physiology and metabolism. Genome-based comparison of drug targets together with quantitative tissue expression analysis allows rational prediction of pharmacology and cross-reactivity of human drugs in animal models thereby improving drug attrition which is an important challenge in the process of drug development.; Here we present a new chromosome level based version of the Göttingen minipig genome together with a comparative transcriptional analysis of tissues with pharmaceutical relevance as basis for translational research. We relied on mapping and assembly of WGS (whole-genome-shotgun sequencing) derived reads to the reference genome of the Duroc pig and predict 19,228 human orthologous protein-coding genes. Genome-based prediction of the sequence of human drug targets enables the prediction of drug cross-reactivity based on conservation of binding sites. We further support the finding that the genome of Sus scrofa contains about ten-times less pseudogenized genes compared to other vertebrates. Among the functional human orthologs of these minipig pseudogenes we found HEPN1, a putative tumor suppressor gene. The genomes of Sus scrofa, the Tibetan boar, the African Bushpig, and the Warthog show sequence conservation of all inactivating HEPN1 mutations suggesting disruption before the evolutionary split of these pig species. We identify 133 Sus scrofa specific, conserved long non-coding RNAs (lncRNAs) in the minipig genome and show that these transcripts are highly conserved in the African pigs and the Tibetan boar suggesting functional significance. Using a new minipig specific microarray we show high conservation of gene expression signatures in 13 tissues with biomedical relevance between humans and adult minipigs. We underline this relationship for minipig and human liver where we could demonstrate similar expression levels for most phase I drug-metabolizing enzymes. Higher expression levels and metabolic activities were found for FMO1, AKR/CRs and for phase II drug metabolizing enzymes in minipig as compared to human. The variability of gene expression in equivalent human and minipig tissues is considerably higher in minipig organs, which is important for study design in case a human target belongs to this variable category in the minipig. The first analysis of gene expression in multiple tissues during development from young to adult shows that the majority of transcriptional programs are concluded four weeks after birth. This finding is in line with the advanced state of human postnatal organ development at comparative age categories and further supports the minipig as model for pediatric drug safety studies.; Genome based assessment of sequence conservation combined with gene expression data in several tissues improves the translational value of the minipig for human drug development. The genome and gene expression data presented here are important resources for researchers using the minipig as model for biomedical research or commercial breeding. Potential impact of our data for comparative genomics, translational research, and experimental medicine are discussed

An exquisitely deep view of quenching galaxies through the gravitational lens: Stellar population, morphology, and ionized gas

This work presents an in-depth analysis of four gravitationally lensed red galaxies at z = 1.6-3.2. The sources are magnified by factors of 2.7-30 by foreground clusters, enabling spectral and morphological measurements that are otherwise challenging. Our sample extends below the characteristic mass of the stellar mass function and is thus more representative of the quiescent galaxy population at z > 1 than previous spectroscopic studies. We analyze deep VLT/X-SHOOTER spectra and multi-band Hubble Space Telescope photometry that cover the rest-frame UV-to-optical regime. The entire sample resembles stellar disks as inferred from lensing-reconstructed images. Through stellar population synthesis analysis we infer that the targets are young (median age = 0.1-1.2 Gyr) and formed 80% of their stellar masses within 0.07-0.47 Gyr. Mg II $\lambda\lambda 2796,2803$ absorption is detected across the sample. Blue-shifted absorption and/or redshifted emission of Mg II is found in the two youngest sources, indicative of a galactic-scale outflow of warm ($T\sim10^{4}$ K) gas. The [O III] $\lambda5007$ luminosity is higher for the two young sources (median age less than 0.4 Gyr) than the two older ones, perhaps suggesting a decline in nuclear activity as quenching proceeds. Despite high-velocity ($v\approx1500$ km s$^{-1}$) galactic-scale outflows seen in the most recently quenched galaxies, warm gas is still present to some extent long after quenching. Altogether our results indicate that star formation quenching at high redshift must have been a rapid process (< 1 Gyr) that does not synchronize with bulge formation or complete gas removal. Substantial bulge growth is required if they are to evolve into the metal-rich cores of present-day slow-rotators.Comment: Accepted for publication in the Astrophysical Journal. 37 pages, 20 figures, 10 table

Sunyaev-Zel'dovich observations of galaxy clusters out to the virial radius with the Arcminute Microkelvin Imager

We present observations using the Small Array of the Arcminute Microkelvin Imager (AMI; 14-18 GHz) of four Abell and three MACS clusters spanning 0.171-0.686 in redshift. We detect Sunyaev-Zel'dovich (SZ) signals in five of these without any attempt at source subtraction, although strong source contamination is present. With radio-source measurements from high-resolution observations, and under the assumptions of spherical $\beta$-model, isothermality and hydrostatic equilibrium, a Bayesian analysis of the data in the visibility plane detects extended SZ decrements in all seven clusters over and above receiver noise, radio sources and primary CMB imprints. Bayesian evidence ratios range from 10^{11}:1 to 10^{43}:1 for six of the clusters and 3000:1 for one with substantially less data than the others. We present posterior probability distributions for, e.g., total mass and gas fraction averaged over radii internal to which the mean overdensity is 1000, 500 and 200, r_200 being the virial radius. Reaching r_200 involves some extrapolation for the nearer clusters but not for the more-distant ones. We find that our estimates of gas fraction are low (compared with most in the literature) and decrease with increasing radius. These results appear to be consistent with the notion that gas temperature in fact falls with distance (away from near the cluster centre) out to the virial radius.Comment: 18 pages, 10 figures, submitted to MNRAS (updated authors and fixed Figure 1

Precision modeling of JWST's first cluster lens SMACSJ0723.3-7327

Exploiting the fundamentally achromatic nature of gravitational lensing, we present a lens model for the massive galaxy cluster SMACSJ0723.3-7323 (SMACS J0723, z=0.388) that significantly improves upon earlier work. Building on strong-lensing constraints identified in prior Hubble Space Telescope (HST) observations, the mass model utilizes 21 multiple-image systems, 16 of which were newly discovered in Early Release Observation (ERO) data from the James Webb Space Telescope (JWST). The resulting lens model maps the cluster mass distribution to an RMS spatial precision of 1.08'' and is publicly available at https://www.dropbox.com/sh/3iatmz5k4hafzqf/AAAh0JvLgpBVoLp6qsxYZkFGa?dl=0 . Consistent with previous analyses, our study shows SMACSJ0723.3-7323 to be well described by a single large-scale component centered on the location of the brightest cluster galaxy, however JWST data point to the need for two additional diffuse components west of the cluster, which in turn accounts for all the currently identified multiply imaged systems. A comparison of the galaxy distribution, the mass distribution, and gas distribution in the core of SMACS0723 based on HST, JWST, and Chandra data reveals a fairly concentrated regular elliptical profile along with tell-tale signs of recent merger activity, possibly proceeding aligned closely to our line of sight. The exquisite sensitivity of JWST's NIRCAM reveals in spectacular fashion both the extended intra-cluster-light distribution and numerous star-forming clumps in magnified background galaxies. The high-precision lens model derived here for SMACSJ0723-7323 demonstrates impressively the power of combining HST and JWST data for unprecedented studies of structure formation and evolution in the distant Universe.Comment: Updated to match the version submitted to ApJ - analysis, results and conclusions are unchanged. Link to the lensing outputs: https://www.dropbox.com/sh/3iatmz5k4hafzqf/AAAh0JvLgpBVoLp6qsxYZkFGa?dl=

Biodiversity-stability relationships strengthen over time in a long-term grassland experiment.

Numerous studies have demonstrated that biodiversity drives ecosystem functioning, yet how biodiversity loss alters ecosystems functioning and stability in the long-term lacks experimental evidence. We report temporal effects of species richness on community productivity, stability, species asynchrony, and complementarity, and how the relationships among them change over 17 years in a grassland biodiversity experiment. Productivity declined more rapidly in less diverse communities resulting in temporally strengthening positive effects of richness on productivity, complementarity, and stability. In later years asynchrony played a more important role in increasing community stability as the negative effect of richness on population stability diminished. Only during later years did species complementarity relate to species asynchrony. These results show that species complementarity and asynchrony can take more than a decade to develop strong stabilizing effects on ecosystem functioning in diverse plant communities. Thus, the mechanisms stabilizing ecosystem functioning change with community age

Biodiversity–stability relationships strengthen over time in a long-term grassland experiment

Numerous studies have demonstrated that biodiversity drives ecosystem functioning, yet how biodiversity loss alters ecosystems functioning and stability in the long-term lacks experimental evidence. We report temporal effects of species richness on community productivity, stability, species asynchrony, and complementarity, and how the relationships among them change over 17 years in a grassland biodiversity experiment. Productivity declined more rapidly in less diverse communities resulting in temporally strengthening positive effects of richness on productivity, complementarity, and stability. In later years asynchrony played a more important role in increasing community stability as the negative effect of richness on population stability diminished. Only during later years did species complementarity relate to species asynchrony. These results show that species complementarity and asynchrony can take more than a decade to develop strong stabilizing effects on ecosystem functioning in diverse plant communities. Thus, the mechanisms stabilizing ecosystem functioning change with community age

The KALEIDOSCOPE survey : A new strong and weak gravitational lensing view of the massive galaxy cluster MACS J1423.8+2404

We present a combined strong and weak gravitational-lensing analysis of the massive galaxy cluster MACS J1423.8+2404 (z = 0.545, MACS J1423 hereafter), one of the most dynamically relaxed and massive cool-core clusters discovered in the MAssive Cluster Survey at z > 0.5. We combine high-resolution imaging from the Hubble Space Telescope (HST) in the F606W, F814W, and F160W pass-bands with spectroscopic observations taken as part of the KALEIDOSCOPE survey with the Multi-Unit Spectroscopic Explorer mounted on the Very Large Telescope. Our strong lensing analysis of the mass distribution in the cluster core is constrained by four multiple-image systems (17 individual images) within redshift range 1.779 < z < 2.840. Our weak lensing analysis of the cluster outskirts, confined to the HST field of view, is based on a background galaxy catalogue with a density of 57 gal arcmin−2. We measure a projected mass of M(R < 200 kpc) = (1.6 ± 0.05) × 1014 M from our strong lensing model, and a projected mass of M(R < 640 kpc) = (6.6 ± 0.6) × 1014 M when combining with our the weak lensing constraints. Our analysis of the cluster mass distribution yields no evidence of substructures, confirming the dynamically relaxed state of MACS J1423. Our work sets the stage for future analysis of MACS J1423 in the upcoming Canadian Near-Infrared Imager and Stiltless Spectrograph Unbiased Cluster Survey for the JWST