Isolation and Identification of an Antimutagenic Phthalate Derivative Compound from Octopus (Paraoctopus limaculatus)

Purpose: To isolate and evaluate the antimutagenic properties of compounds previously identified in octopus (Paraoctopus limaculatus).Methods: Octopus fractions, previously obtained by a sequential thin layer chromatography (TLC) procedure, were subjected to further fractionation by TLC and their anti-mutagenic activity monitored using Salmonella tester strains TA98 and TA100 with metabolic activation (S9) in Ames test. The isolated fractions were subjected to structural studies by Fourier transformed infrared spectroscopy (FTIR), nuclear magnetic resonance (1H and 13C NMR), and gas chromatography-mass spectrometry.Results: Five new fractions were obtained from a previously isolated and reported anti-mutagenic octopus fraction. Fractions RB21321b2 and RB21321b3 inhibited > 80 % of the mutagenicity induced by 500 ng AFB1 on both tester strains and were selected for chemical/structural characterization. Data from IR and 1H and 13C NMR suggested the presence of phthalate type of compounds. GC-MS analysis revealed 278 m/z for both fractions which is consistent with a butyl isobutyl phthalate structure.Conclusion: Based on the findings, the compound responsible for the high anti-mutagenic activity of the isolated fraction from octopus is 1-butyl-2-isobutyl-phthalate.Keywords: Octopus, Anti-Mutagenic, Paraoctopus limaculatus, 1-Butyl-2-isobutyl-phthalat

Nuevas evidencias acerca de la presencia de una banda de cizallamiento en la secuencia metapelitica de la Formación Silgará, sector Aratoca-Pescadero (region suroccidental del Macizo de Santander)

La secuencia metamórfica de la Formación Silgará que aflora en el sector Pescadero-Aratoca (región suroccidental del Macizo de Santander), presenta evidencias de haber sufrido un importante cizallamiento durante su etapa de exhumación, el cual afectó gran parte de las rocas metamórficas de las zonas del granate y de la estaurolita-distena. Esta deformación dúctil de carácter extensional generó estructuras tales como lineaciones, sombras de presión, zonas de milonitización, pliegues isoclinales recumbentes, pliegues sin raíz, boudines, así como la elongación de cristales de granate y el truncamiento de su zonación química, lo cual apunta a un colapso gravitacional del paleoorógeno Caledoniano en dirección techo hacia el Este. Estos elementos deformacionales, sumados a las evidencias de una importante circulación de fluidos hidrotermales, hacen que esta región del Macizo de Santander merezca especial atención para trabajos de exploración de depósitos mesotermales, especialmente por presentar condiciones favorables para la removilización de oro.The metamorphic sequence of the Silgara Formation that crops out in Pescadero-Aratoca area (southwestern region of the Santander Massif), presents evidences of having suffered an important shear deformation during its exhumation path, which affected great part of the metamorphic rocks from the garnet and staurolitekyanite zones. This ductile deformation of extensional character generated structures such as lineations, pressure shades, milonitization zones, isoclinal recumbent folds, rootless folds, boudins, as well as the elongation of garnet crystals and the truncation of their chemical zonation, which suggest a proccess of gravitational collapse with an East motion, that affected the Caledonian paleo-orogen. These deformational elements, along with the evidences of an important circulation of hydrothermal fluids, make this region of the Santander Massif an important place to pay attention to explore mesotermal deposits, especially for its favorable conditions for the removization of gold

Add-on inhaled budesonide in the treatment of hospitalised patients with COVID-19 : a randomised clinical trial

SARS-CoV-2 vaccines have been extremely effective to reduce the incidence of severe COVID19 [1-3], but effective and safe treatments of the acute infection are still limited [4, 5]. An uncontrolled pulmonary inflammatory response to SARS-CoV-2 is considered a key pathogenic mechanism of COVID19 progression [6], so systemic dexamethasone is recommended in severe cases [5, 7]. On the other hand, in very mild patients at home inhaled corticosteroids (ICS) may prevent disease progression [8-11]. Whether ICS prevent disease progression too in patients hospitalised because of COVID19 has not been explored before. Accordingly, we designed an investigator-initiated, open-label, randomised clinical trial (RCT) to explore the efficacy of adding inhaled budesonide to usual care to prevent disease progression in patients hospitalised because of COVID19 pneumonia. We also monitored carefully the safety of this intervention since there are concerns about the use of systemic corticosteroids in other viral (influenza) lung infections [12]

Clinical Practice Guideline on Melanoma From the Spanish Academy of Dermatology and Venereology (AEDV)

El diagnóstico y tratamiento del melanoma en atención especializada es un campo en el que se han producido numerosos cambios. El objetivo de esta guía es ofrecer a los dermatólogos españoles una referencia para resolver las dudas clínicas más frecuentes basándose en la evidencia actual. Para la realización de esta guía se escogió a miembros del Grupo Español de Dermato-Oncología y Cirugía con experiencia en el tratamiento de estos tumores y con interés en participar en la elaboración de la guía. Se hizo una adaptación de las guías de práctica clínica existentes mediante el método ADAPTE: inicialmente se resumió el proceso de atención y se elaboraron las preguntas clínicas relevantes. Se seleccionaron las guías mejor puntuadas mediante el instrumento AGREE II, realizando la búsqueda de las respuestas en dichas guías y elaborando las recomendaciones. Finalmente se sometió la guía a revisión externa. La guía se estructuró a partir de 21 preguntas clínicas que fueron seleccionadas por su relevancia, dado que se centran en aspectos que pueden plantear decisiones difíciles en el manejo del melanoma, y se han respondido empleando la evidencia obtenida de las mejores guías existentes. Entre las limitaciones de esta guía merece reseñarse que la evidencia es escasa para responder a algunas preguntas. En algunos aspectos el cambio es rápido y exige una actualización frecuente de la guía. Esta guía responde a preguntas habituales sobre el manejo del melanoma en la práctica clínica diaria, sirviendo a los dermatólogos como referencia en la toma de decisiones, siempre teniendo presente los recursos y preferencias del paciente

VOLUMEN 25, NÚMERO 40 (2003)

TERMOCRONOLOGÍA K-Ar EN ILLITAS: ASPECTOS METODOLÓGICOS. Mantilla Figueroa, L. C.CINÉTICA DE LA NUCLEACIÓN Y EL CRECIMIENTO DEL GRANATE EN LAS ROCAS METAPELÍTICAS DE LA FORMACIÓN SILGARÁ, REGIÓN SUROCCIDENTAL DEL MACIZO DE SANTANDER. Ríos Reyes, C., Gélvez Llanes, J., Márquez Romero, R. y Castellanos Alarcón, O.INTRODUCCIÓN A LA GEOLOGÍA DEL SECTOR VÉLEZ–BOLÍVAR–GUAVATÁ (DPTO. DE SANTANDER, COLOMBIA) Y SU IMPORTANCIA PARA LA EXPLORACIÓN DE DEPÓSITOSHIDROTERMALES. Mantilla Figueroa, L. C.; Cruz G. L. E.; Colegial G. J. D.RECOGNITION OF ANCIENT SHOREFACE DEPOSITS. FACIES, FACIES SUCESSIONS, AND ASSOCIATIONS. AN EXAMPLE FROM THE CRETACEOUS GALLUP CLASTIC WEDGE, NEW MÉXICO. Álvarez Bastos, MNUEVAS EVIDENCIAS ACERCA DE LA PRESENCIA DE UNA BANDA DE CIZALLAMIENTO EN LA SECUENCIA METAPELÍTICA DE LA FORMACIÓN SILGARÁ, SECTOR ARATOCA-PESCADERO (REGION SUROCCIDENTAL DEL MACIZO DE SANTANDER). Mantilla Figueroa, L. C.; Ríos Reyes, C. A.; Gélvez Llanes, J. R.; Márquez Romero, R. E.; Ordóñez Calderón, J. C.; Cepeda Espitia, S.LOS DELIRIOS: UN YACIMIENTO HIDROTERMAL DE ORO Y PLATA EN LA REGIÓN DE VETAS, SANTANDER (COLOMBIA). García Ramírez, C. A., Uribe Portilla, E.MINERALOGÍA, GEOQUÍMICA Y ALGUNOS ASPECTOS GENÉTICOS DE LA MINA EL DIAMANTE - NARIÑO (COLOMBIA). Molano, J.C. and Shimazaki, H.COMPARATIVE FLUID INCLUSION STUDIES OF THE SAN MARTÍN SKARN (MÉXICO) AND THE EPITHERMAL COMSTOCK LODE (USA) AND PACHUCA-REAL DEL MONTE (MÉXICO) DEPOSITS. Campos Álvarez, N. O

VOLUMEN 25, NÚMERO 40 (2003)

TERMOCRONOLOGÍA K-Ar EN ILLITAS: ASPECTOS METODOLÓGICOS. Mantilla Figueroa, L. C.CINÉTICA DE LA NUCLEACIÓN Y EL CRECIMIENTO DEL GRANATE EN LAS ROCAS METAPELÍTICAS DE LA FORMACIÓN SILGARÁ, REGIÓN SUROCCIDENTAL DEL MACIZO DE SANTANDER. Ríos Reyes, C., Gélvez Llanes, J., Márquez Romero, R. y Castellanos Alarcón, O.INTRODUCCIÓN A LA GEOLOGÍA DEL SECTOR VÉLEZ–BOLÍVAR–GUAVATÁ (DPTO. DE SANTANDER, COLOMBIA) Y SU IMPORTANCIA PARA LA EXPLORACIÓN DE DEPÓSITOSHIDROTERMALES. Mantilla Figueroa, L. C.; Cruz G. L. E.; Colegial G. J. D.RECOGNITION OF ANCIENT SHOREFACE DEPOSITS. FACIES, FACIES SUCESSIONS, AND ASSOCIATIONS. AN EXAMPLE FROM THE CRETACEOUS GALLUP CLASTIC WEDGE, NEW MÉXICO. Álvarez Bastos, MNUEVAS EVIDENCIAS ACERCA DE LA PRESENCIA DE UNA BANDA DE CIZALLAMIENTO EN LA SECUENCIA METAPELÍTICA DE LA FORMACIÓN SILGARÁ, SECTOR ARATOCA-PESCADERO (REGION SUROCCIDENTAL DEL MACIZO DE SANTANDER). Mantilla Figueroa, L. C.; Ríos Reyes, C. A.; Gélvez Llanes, J. R.; Márquez Romero, R. E.; Ordóñez Calderón, J. C.; Cepeda Espitia, S.LOS DELIRIOS: UN YACIMIENTO HIDROTERMAL DE ORO Y PLATA EN LA REGIÓN DE VETAS, SANTANDER (COLOMBIA). García Ramírez, C. A., Uribe Portilla, E.MINERALOGÍA, GEOQUÍMICA Y ALGUNOS ASPECTOS GENÉTICOS DE LA MINA EL DIAMANTE - NARIÑO (COLOMBIA). Molano, J.C. and Shimazaki, H.COMPARATIVE FLUID INCLUSION STUDIES OF THE SAN MARTÍN SKARN (MÉXICO) AND THE EPITHERMAL COMSTOCK LODE (USA) AND PACHUCA-REAL DEL MONTE (MÉXICO) DEPOSITS. Campos Álvarez, N. O

Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components. Dendritic cells (DCs) play a key role in the defense against viral infections, for instance plasmacytoid DCs (pDCs), have the capacity to produce vast amounts of interferon-alpha (IFN-α). In COVID-19 there is a deficit in DC numbers and IFN-α production, which has been associated with disease severity. In this work, we described that in addition to the DC deficiency, several DC activation and homing markers were altered in acute COVID-19 patients, which were associated with multiple inflammatory markers. Remarkably, previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+ myeloid DCs and pDCs seven months after SARS-CoV-2 infection. Moreover, the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients, while no restoration of integrin β7 and indoleamine 2,3-dyoxigenase (IDO) levels were observed. These findings contribute to a better understanding of the immunological sequelae of COVID-19

SARS-CoV-2 viral load in nasopharyngeal swabs is not an independent predictor of unfavorable outcome

The aim was to assess the ability of nasopharyngeal SARS-CoV-2 viral load at first patient’s hospital evaluation to predict unfavorable outcomes. We conducted a prospective cohort study including 321 adult patients with confirmed COVID-19 through RT-PCR in nasopharyngeal swabs. Quantitative Synthetic SARS-CoV-2 RNA cycle threshold values were used to calculate the viral load in log10 copies/mL. Disease severity at the end of follow up was categorized into mild, moderate, and severe. Primary endpoint was a composite of intensive care unit (ICU) admission and/or death (n = 85, 26.4%). Univariable and multivariable logistic regression analyses were performed. Nasopharyngeal SARS-CoV-2 viral load over the second quartile (≥ 7.35 log10 copies/mL, p = 0.003) and second tertile (≥ 8.27 log10 copies/mL, p = 0.01) were associated to unfavorable outcome in the unadjusted logistic regression analysis. However, in the final multivariable analysis, viral load was not independently associated with an unfavorable outcome. Five predictors were independently associated with increased odds of ICU admission and/or death: age ≥ 70 years, SpO2, neutrophils > 7.5 × 103/µL, lactate dehydrogenase ≥ 300 U/L, and C-reactive protein ≥ 100 mg/L. In summary, nasopharyngeal SARS-CoV-2 viral load on admission is generally high in patients with COVID-19, regardless of illness severity, but it cannot be used as an independent predictor of unfavorable clinical outcome

Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry.

BACKGROUND: The Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF) is an ongoing prospective noninterventional registry, which is providing important information on the baseline characteristics, treatment patterns, and 1-year outcomes in patients with newly diagnosed non-valvular atrial fibrillation (NVAF). This report describes data from Indian patients recruited in this registry. METHODS AND RESULTS: A total of 52,014 patients with newly diagnosed AF were enrolled globally; of these, 1388 patients were recruited from 26 sites within India (2012-2016). In India, the mean age was 65.8 years at diagnosis of NVAF. Hypertension was the most prevalent risk factor for AF, present in 68.5% of patients from India and in 76.3% of patients globally (P < 0.001). Diabetes and coronary artery disease (CAD) were prevalent in 36.2% and 28.1% of patients as compared with global prevalence of 22.2% and 21.6%, respectively (P < 0.001 for both). Antiplatelet therapy was the most common antithrombotic treatment in India. With increasing stroke risk, however, patients were more likely to receive oral anticoagulant therapy [mainly vitamin K antagonist (VKA)], but average international normalized ratio (INR) was lower among Indian patients [median INR value 1.6 (interquartile range {IQR}: 1.3-2.3) versus 2.3 (IQR 1.8-2.8) (P < 0.001)]. Compared with other countries, patients from India had markedly higher rates of all-cause mortality [7.68 per 100 person-years (95% confidence interval 6.32-9.35) vs 4.34 (4.16-4.53), P < 0.0001], while rates of stroke/systemic embolism and major bleeding were lower after 1 year of follow-up. CONCLUSION: Compared to previously published registries from India, the GARFIELD-AF registry describes clinical profiles and outcomes in Indian patients with AF of a different etiology. The registry data show that compared to the rest of the world, Indian AF patients are younger in age and have more diabetes and CAD. Patients with a higher stroke risk are more likely to receive anticoagulation therapy with VKA but are underdosed compared with the global average in the GARFIELD-AF. CLINICAL TRIAL REGISTRATION-URL: http://www.clinicaltrials.gov. Unique identifier: NCT01090362