A importância do m-learning para os cursos de formação

Propõe investigar quais são os fatores que impedem a implementação de ambiente de ensino virtual em aplicativos em dispositivos móveis.26 páginasEducação e DocênciaNível Macro: Teoria e Modelo

Impact of exposure of methicillin-resistant Staphylococcus aureus to polyhexanide in vitro and in vivo.

Staphylococcus aureus (MRSA) resistant to decolonization agents such as mupirocin and chlorhexidine increase the need to develop alternative decolonization molecules. The absence of reported adverse reactions and bacterial resistance to polyhexanide makes it an excellent choice as topical antiseptic. In the present study we evaluated the in vitro and in vivo capacity to generate strains with reduced polyhexanide susceptibility and cross-resistance with chlorhexidine and/or antibiotics currently used in clinic. Here we report the in vitro emergence of reduced-susceptibility to polyhexanide by prolonged-stepwise exposure to low concentrations in broth culture. Reduced susceptibility to polyhexanide was associated with genomic changes in the mprF and purR genes, and with concomitant decreased susceptibility to daptomycin and other cell-wall active antibiotics. However, the in vitro emergence of reduced-susceptibility to polyhexanide did not result in cross-resistance to chlorhexidine antiseptic. During in vivo polyhexanide clinical decolonization treatment, neither polyhexanide reduced-susceptibility nor chlorhexidine cross-resistance were observed. Together, these observations suggest that polyhexanide could be used safely for decolonisation of carriers of chlorhexidine-resistant S. aureus strains but highlight the need for careful use of polyhexanide at low antiseptic concentrations

Functional properties of milk drinks flavored with mangaba pulp and enriched with passion fruit bark flour

Dairy foods including dairy based drinks play an important role in human nutrition. The flour of the passion fruit peel has high potential for use in the enrichment dairy drinks improving the nutritional and technological qualities besides an alternative to reduce waste by-products in the food industry. Thus, this study aimed to evaluate the physical and chemical parameters, texture, color, chemical composition, scanning electron microscopy (SEM), phenolic compounds, antioxidant, viability of lactic bacteria and sensory profile of milk drinks added mangaba pulp and passion fruit peel flour of the (FPFP). Four milk drinks formulations were processed with concentrations of 5; 10; 15 and 20% mangaba pulp and 1% of passion fruit peel flour. The dairy beverages showed results physical and chemical, microbiological and sensory consistent with those described in the literature and as expected, with a high sensory acceptability of milk drinks with increased by 10% mangaba pulp.Keywords: Color, fermented milk, scanning electron microscopy (SEM), viabilit

Didática no online: novas coreografias e seus olhares

A didática no online traz uma configuração inovadora e peculiar no caminho das práticas e nas criações pedagógicas para o processo de ensino e aprendizagem. O presente painel estrutura-se na reflexão e nas experiências do professor como coreógrafo estratégico na convergência do online com o presencial, provocando oportunidades para as aprendizagens profundas e significativas. As categorias presentes na fundamentação teórica são: interação, estratégias, redes, cibercultura e assincronicidade. A proposta é parte das investigações em curso sobre as tendências da didática nos contextos online, as discussões apresentadas refletem a produção científica no campo da didática do contexto brasileiro e europeu. Os caminhos metodológicos englobam a análise qualitativa e quantitativa suportada por referenciais bibliográficos, reflexões e discussões em rede a partir de práticas colaborativas e de coaprendizagem entre as investigadoras. A didática apresentada fundamenta-se em diferentes vertentes teóricas adoptando referenciais específicos. É proposto a continua inferência nas discussões e realizações para a sustentabilidade da didática do assíncrono em convergência do presencial com o online. A partir dos vários eixos teóricos analisados surgem indicadores e variáveis sustentáveis que o painel trará nas propostas que serão apresentadas, nunca finalizadas, mas como elementos de discussão já fundamentados e testados com novos olhares e coreografias que potencializam esse trabalho em sua melhor atualização e gestão nas aprendizagem do século XXI. A didática que as investigadoras envolvidas discutem está numa configuração que considera os formatos oferecidos pelas tecnologias, com sua análise e os processos de didatização desses cenários, trazendo uma intencionalidade pedagógica ainda difícil de ser concretizada nas propostas de ensino e aprendizagem no online.info:eu-repo/semantics/publishedVersio

Clonal diversity and detection of carbapenem resistance encoding genes among multidrug-resistant Acinetobacter baumannii isolates recovered from patients and environment in two intensive care units in a Moroccan hospital

Background Carbapenem-resistant Acinetobacter baumannii has recently been defined by the World Health Organization as a critical pathogen. The aim of this study was to compare clonal diversity and carbapenemase-encoding genes of A. baumannii isolates collected from colonized or infected patients and hospital environment in two intensive care units (ICUs) in Morocco. Methods The patient and environmental sampling was carried out in the medical and surgical ICUs of Mohammed V Military teaching hospital from March to August 2015. All A. baumannii isolates recovered from clinical and environmental samples, were identified using routine microbiological techniques and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Antimicrobial susceptibility testing was performed using disc diffusion method. The carbapenemase-encoding genes were screened for by PCR. Clonal relatedness was analyzed by digestion of the DNA with low frequency restriction enzymes and pulsed field gel electrophoresis (PFGE) and the multi locus sequence typing (MLST) was performed on two selected isolates from two major pulsotypes. Results A total of 83 multidrug-resistant A. baumannii isolates were collected: 47 clinical isolates and 36 environmental isolates. All isolates were positive for the bla OXA51-like and bla OXA23-like genes. The coexistence of bla NDM-1 /bla OXA-23-like and bla OXA 24-like /bla OXA-23-like were detected in 27 (32.5%) and 2 (2.4%) of A. baumannii isolates, respectively. The environmental samples and the fecally-colonized patients were significantly identified (p < 0.05) as the most common sites of isolation of NDM-1-harboring isolates. PFGE grouped all isolates into 9 distinct clusters with two major groups (0007 and 0008) containing up to 59% of the isolates. The pulsotype 0008 corresponds to sequence type (ST) 195 while pulsotype 0007 corresponds to ST 1089.The genetic similarity between the clinical and environmental isolates was observed in 80/83 = 96.4% of all isolates, belonging to 7 pulsotypes. Conclusion This study shows that the clonal spread of environmental A. baumannii isolates is related to that of clinical isolates recovered from colonized or infected patients, being both associated with a high prevalence of the bla OXA23-like and bla NDM-1genes. These findings emphasize the need for prioritizing the bio-cleaning of the hospital environment to control and prevent the dissemination of A. baumannii clonal lineages

Carbapenem Resistance and Acinetobacter baumannii in Senegal: The Paradigm of a Common Phenomenon in Natural Reservoirs

Incidence of carbapenem-resistant Acinetobacter baumannii is rising in several parts of the world. In Africa, data concerning this species and its resistance to carbapenems are limited. The objective of the present study was to identify the presence of A. baumannii carbapenem-resistant encoding genes in natural reservoirs in Senegal, where antibiotic pressure is believed to be low. From October 2010 to January 2011, 354 human head lice, 717 human fecal samples and 118 animal fecal samples were screened for the presence of A. baumannii by real time PCR targeting blaOXA51-like gene. For all samples positive for A. baumannii, the carbapenemase-hydrolysing oxacillinases blaOXA23-like and blaOXA24-like were searched for and sequenced, and the isolates harbouring an oxacillinase were genotyped using PCR amplification and sequencing of recA gene. The presence of A. baumannii was detected in 4.0% of the head lice, in 5.4% of the human stool samples and in 5.1% of the animal stool samples tested. No blaOXA24 gene was detected but six fecal samples and three lice were positive for blaOXA23-like gene. The blaOXA23-like gene isolated in lice was likely a new oxacillinase sequence. Finally, the A. baumannii detected in stools were all of recA genotype 3 and those detected in lice, of recA genotype 4. This study shows for the first time a reservoir of blaOXA23-like-positive gene in human head lice and stool samples in Senegal

French database of children and adolescents with Prader-Willi syndrome

<p>Abstract</p> <p>Background</p> <p>Prader-Willi syndrome (PWS) is a rare multisystem genetic disease leading to severe complications mainly related to obesity. We strongly lack information on the natural history of this complex disease and on what factors are involved in its evolution and its outcome. One of the objectives of the French reference centre for Prader-Willi syndrome set-up in 2004 was to set-up a database in order to make the inventory of Prader-Willi syndrome cases and initiate a national cohort study in the area covered by the centre.</p> <p>Description</p> <p>the database includes medical data of children and adolescents with Prader-Willi syndrome, details about their management, socio-demographic data on their families, psychological data and quality of life of the parents. The tools and organisation used to ensure data collection and data quality in respect of good clinical practice procedures are discussed, and main characteristics of our Prader-Willi population at inclusion are presented.</p> <p>Conclusion</p> <p>this database covering all the aspects of PWS clinical, psychological and social profiles, including familial psychological and quality of life will be a powerful tool for retrospective studies concerning this complex and multi factorial disease and could be a basis for the design of future prospective multicentric studies. The complete database and the Stata.do files are available to any researcher wishing to use them for non-commercial purposes and can be provided upon request to the corresponding author.</p

Institutional shelter to protect adolescent victims of domestic violence: theory or practice?

OBJECTIVE: To understand and analyze, from the perspective of adolescent victims of domestic violence who were cared for in an institution in Campinas-SP, the protective factors to which they are submitted and / or have access. METHOD: This was qualitative research, with data collection occurring through focus groups with 17 adolescents, and semistructured interviews with seven of them; the data analysis was based on content analysis, using a thematic modality. RESULTS: Two themes emerged, entitled Four Walls and Trust. We discuss the context of institutional care, that despite the efforts made contemporaneously, still maintains an authoritarian environment; the importance of the bond and trust established with some employees, acting as protective factors for the adolescents and the protective aspect of religiosity. CONCLUSIONS: It is understood that these considerations should be valued and reinforced through the healthcare services provided to children and adolescents, as they contribute to the promotion of the physical and mental health of this population