358 research outputs found
Cryptic diversity within the major trypanosomiasis vector Glossina fuscipes revealed by molecular markers
Background: The tsetse fly Glossina fuscipes s.l. is responsible for the transmission of approximately 90% of cases of human African trypanosomiasis (HAT) or sleeping sickness. Three G. fuscipes subspecies have been described, primarily based upon subtle differences in the morphology of their genitalia. Here we describe a study conducted across the range of this important vector to determine whether molecular evidence generated from nuclear DNA (microsatellites and gene sequence information), mitochondrial DNA and symbiont DNA support the existence of these taxa as discrete taxonomic units.
Principal Findings: The nuclear ribosomal Internal transcribed spacer 1 (ITS1) provided support for the three subspecies. However nuclear and mitochondrial sequence data did not support the monophyly of the morphological subspecies G. f.fuscipes or G. f. quanzensis. Instead, the most strongly supported monophyletic group was comprised of flies sampled fromEthiopia. Maternally inherited loci (mtDNA and symbiont) also suggested monophyly of a group from Lake Victoria basin and Tanzania, but this group was not supported by nuclear loci, suggesting different histories of these markers. Microsatellite data confirmed strong structuring across the range of G. fuscipes s.l., and was useful for deriving the interrelationship of closely related populations.
Conclusion/Significance: We propose that the morphological classification alone is not used to classify populations of G. fuscipes for control purposes. The Ethiopian population, which is scheduled to be the target of a sterile insect release (SIT) programme, was notably discrete. From a programmatic perspective this may be both positive, given that it may reflect limited migration into the area or negative if the high levels of differentiation are also reflected in reproductive isolation between this population and the flies to be used in the release programme
A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos
Further refinement of culture media is needed to improve the quality of embryos generated in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the impact of reduced nutrient concentrations in culture medium on mouse embryo development, metabolism, and quality as a possible platform for next generation medium formulation. Concentrations of carbohydrates, amino acids, and vitamins could be reduced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard nutrient provision (reduced nutrient medium; RN). Addition of pyruvate and L-lactate (+PL) to RN at 50% of standard concentrations restored blastocyst development, hatching, and cell number. In addition, blastocysts produced in RN\u2009+PL contained more ICM cells and ATP than blastocysts cultured in our control (100% nutrient) medium; however, metabolic activity was altered. Similarly, embryos produced in the RN medium with elevated (50% control) concentrations of pyruvate and lactate in the first step medium and EAA and Glu in the second step medium were competent to implant and develop into fetuses at
a similar rate as embryos produced in the control medium. This novel approach to culture medium formulation could help define the optimal nutrient requirements of embryos in culture and provide a means of shifting metabolic activity towards the utilization of specific metabolic pathways that may be beneficial for embryo viability
DT/T beyond linear theory
The major contribution to the anisotropy of the temperature of the Cosmic
Microwave Background (CMB) radiation is believed to come from the interaction
of linear density perturbations with the radiation previous to the decoupling
time. Assuming a standard thermal history for the gas after recombination, only
the gravitational field produced by the linear density perturbations present on
a universe can generate anisotropies at low z (these
anisotropies would manifest on large angular scales). However, secondary
anisotropies are inevitably produced during the nonlinear evolution of matter
at late times even in a universe with a standard thermal history. Two effects
associated to this nonlinear phase can give rise to new anisotropies: the
time-varying gravitational potential of nonlinear structures (Rees-Sciama RS
effect) and the inverse Compton scattering of the microwave photons with hot
electrons in clusters of galaxies (Sunyaev-Zeldovich SZ effect). These two
effects can produce distinct imprints on the CMB temperature anisotropy. We
discuss the amplitude of the anisotropies expected and the relevant angular
scales in different cosmological scenarios. Future sensitive experiments will
be able to probe the CMB anisotropies beyong the first order primary
contribution.Comment: plain tex, 16 pages, 3 figures. Proceedings of the Laredo Advance
School on Astrophysics "The universe at high-z, large-scale structure and the
cosmic microwave background". To be publised by Springer-Verla
Detecting ground level enhancements using soil moisture sensor networks
Ground level enhancements (GLEs) are space weather events that pose a potential hazard to the aviation environment through single event effects in avionics and increased dose to passengers and crew. The existing ground level neutron monitoring network provides continuous and well-characterized measurements of the radiation environment. However, there are only a few dozen active stations worldwide, and there has not been a UK-based station for several decades. Much smaller neutron detectors are increasingly deployed throughout the world with the purpose of using secondary neutrons from cosmic rays to monitor local soil moisture conditions (COSMOS). Space weather signals from GLEs and Forbush decreases have been identified in COSMOS data. Monte Carlo simulations of atmospheric radiation propagation show that a single COSMOS detector is sufficient to detect the signal of a medium-strength (10%–100% increase above background) GLE at high statistical significance, including at fine temporal resolution. Use of fine temporal resolution would also provide a capability to detect Terrestrial Gamma Ray Flashes (via secondary neutrons) which are produced by certain lightning discharges and which can provide a hazard to aircraft, particularly in tropical regions. We also show how the COsmic-ray Soil Moisture Observing System-UK detector network could be used to provide warnings at the International Civil Aviation Organization “Moderate” and “Severe” dose rate thresholds at aviation altitudes, and how multiple-detector hubs situated at strategic UK locations could detect a small GLE at high statistical significance and infer crucial information on the nature of the primary spectrum
Temporal, spatial, and structural patterns of adult trembling aspen and white spruce mortality in Quebec's boreal forest
Temporal, spatial, and structural patterns of adult trembling aspen (Populus tremuloides Michx.) and white spruce (Picea glauca (Moench) Voss) mortality were studied in intact 150-year-old stands in the southwestern boreal forest of Quebec. For both species, mortality decreases (number of dead trees/total number of trees) with distance from the lake edge until 100-150 m, from which point it slightly increases. Strong peaks in mortality were found for 40- to 60-year-old aspen mainly between 1974 and 1992. Such mortality in relatively young aspen is likely related to competition for light from the dominant canopy trees. Also, the recruitment of this young aspen cohort is presumably the result of a stand breakup that occurred when the initial aspen-dominated stand was between 90 and 110 years old. For spruce, strong peaks in mortality were found in 110- to 150-year-old trees and they occurred mainly after 1980. No clear explanation could be found for these peaks, but we suggest that they may be related to senescence or weakening of the trees following the last spruce budworm outbreak. Suppressed and codominant aspen had a much higher mortality ratio than spruce in the same height class, while more surprisingly, no difference in mortality rate was found between dominant trees of the two species. Most spruce trees were found as standing dead, which leads us to reject the hypothesis that windthrow is an important cause of mortality for spruce in our forests
Search for W' bosons decaying to an electron and a neutrino with the D0 detector
This Letter describes the search for a new heavy charged gauge boson W'
decaying into an electron and a neutrino. The data were collected with the D0
detector at the Fermilab Tevatron proton-antiproton Collider at a
center-of-mass energy of 1.96 TeV, and correspond to an integrated luminosity
of about 1 inverse femtobarn. Lacking any significant excess in the data in
comparison with known processes, an upper limit is set on the production cross
section times branching fraction, and a W' boson with mass below 1.00 TeV can
be excluded at the 95% C.L., assuming standard-model-like couplings to
fermions. This result significantly improves upon previous limits, and is the
most stringent to date.Comment: submitted to Phys. Rev. Let
Measurement of the ratios of the Z/G* + >= n jet production cross sections to the total inclusive Z/G* cross section in ppbar collisions at sqrt(s) = 1.96 TeV
We present a study of events with Z bosons and jets produced at the Fermilab
Tevatron Collider in ppbar collisions at a center of mass energy of 1.96 TeV.
The data sample consists of nearly 14,000 Z/G* -> e+e- candidates corresponding
to the integrated luminosity of 0.4 fb-1 collected using the D0 detector.
Ratios of the Z/G* + >= n jet cross sections to the total inclusive Z/G* cross
section have been measured for n = 1 to 4 jet events. Our measurements are
found to be in good agreement with a next-to-leading order QCD calculation and
with a tree-level QCD prediction with parton shower simulation and
hadronization.Comment: 7 pages, 2 figures, slightly modified, submitted to Phys. Lett.
Measurement of the Isolated Photon Cross Section in p-pbar Collisions at sqrt{s}=1.96 TeV
The cross section for the inclusive production of isolated photons has been
measured in p anti-p collisions at sqrt{s}=1.96 TeV with the D0 detector at the
Fermilab Tevatron Collider. The photons span transverse momenta 23 to 300 GeV
and have pseudorapidity |eta|<0.9. The cross section is compared with the
results from two next-to-leading order perturbative QCD calculations. The
theoretical predictions agree with the measurement within uncertainties.Comment: 7 pages, 5 figures, submitted to Phys.Lett.
- …