Do Clonal Plants Show Greater Division of Labour Morphologically and Physiologically at Higher Patch Contrasts?

When growing in reciprocal patches in terms of availability of different resources, connected ramets of clonal plants will specialize to acquire and exchange locally abundant resources more efficiently. This has been termed division of labour. We asked whether division of labour can occur physiologically as well as morphologically and will increase with patch contrasts.We subjected connected and disconnected ramet pairs of Potentilla anserina to Control, Low, Medium and High patch contrast by manipulating light and nutrient levels for ramets in each pair. Little net benefit of inter-ramet connection in terms of biomass was detected. Shoot-root ratio did not differ significantly between paired ramets regardless of connection under Control, Low and Medium. Under High, however, disconnected shaded ramets with ample nutrients showed significantly larger shoot-root ratios (2.8∼6.5 fold) than fully-lit but nutrient-deficient ramets, and than their counterparts under any other treatment; conversely, fully-lit but nutrient-deficient ramets, when connected to shaded ramets with ample nutrients, had significantly larger shoot-root ratios (2.0∼4.9 fold) than the latter and than their counterparts under any other treatment. Only under High patch contrast, fully-lit ramets, if connected to shaded ones, had 8.9% higher chlorophyll content than the latter, and 22.4% higher chlorophyll content than their isolated counterparts; the similar pattern held for photosynthetic capacity under all heterogeneous treatments.Division of labour in clonal plants can be realized by ramet specialization in morphology and in physiology. However, modest ramet specialization especially in morphology among patch contrasts may suggest that division of labour will occur when the connected ramets grow in reciprocal patches between which the contrast exceeds a threshold. Probably, this threshold patch contrast is the outcome of the clone-wide cost-benefit tradeoff and is significant for risk-avoidance, especially in the disturbance-prone environments

Inter-and intraspecific variation in fern mating systems after long-distance colonization: the importance of selfing

<p>Abstract</p> <p>Background</p> <p>Previous studies on the reproductive biology of ferns showed that mating strategies vary among species, and that polyploid species often show higher capacity for self-fertilization than diploid species. However, the amount of intraspecific variation in mating strategy and selfing capacity has only been assessed for a few species. Yet, such variation may have important consequences during colonization, as the establishment of any selfing genotypes may be favoured after long-distance dispersal (an idea known as Baker's law).</p> <p>Results</p> <p>We examined intra-and interspecific variation in potential for self-fertilization among four rare fern species, of which two were diploids and two were tetraploids: <it>Asplenium scolopendrium </it>(2n), <it>Asplenium trichomanes </it>subsp. <it>quadrivalens </it>(4n), <it>Polystichum setiferum </it>(2n) and <it>Polystichum aculeatum </it>(4n). Sporophyte production was tested at different levels of inbreeding, by culturing gametophytes in isolation, as well as in paired cultures with a genetically different gametophyte. We tested gametophytes derived from various genetically different sporophytes from populations in a recently planted forest colonized through long-distance dispersal (Kuinderbos, the Netherlands), as well as from older, less disjunct populations.</p> <p>Sporophyte production in isolation was high for Kuinderbos genotypes of all four species. Selfing capacity did not differ significantly between diploids and polyploids, nor between species in general. Rather selfing capacity differed between genotypes within species. Intraspecific variation in mating system was found in all four species. In two species one genotype from the Kuinderbos showed enhanced sporophyte production in paired cultures. For the other species, including a renowned out crosser, selfing capacity was consistently high.</p> <p>Conclusions</p> <p>Our results for four different species suggest that intraspecific variation in mating system may be common, at least among temperate calcicole ferns, and that genotypes with high selfing capacity may be present among polyploid as well as diploid ferns. The surprisingly high selfing capacity of all genotypes obtained from the Kuinderbos populations might be due to the isolated position of these populations. These populations may have established through single-spore colonization, which is only possible for genotypes capable of self-fertilization. Our results therewith support the idea that selection for selfing genotypes may occur during long-distance colonization, even in normally outcrossing, diploid ferns.</p

Testing for disconnection and distance effects on physiological self-recognition within clonal fragments of Potentilla reptans

Evidence suggests that belowground self-recognition in clonal plants can be disrupted between sister ramets by the loss of connections or long distances within a genet. However, these results may be confounded by severing connections between ramets in the setups. Using Potentilla reptans, we examined severance effects in a setup that grew ramet pairs with connections either intact or severed. We showed that severance generally reduced new stolon mass but had no effect on root allocation of ramets. However, it did reduce root mass of younger ramets of the pairs. We also explored evidence for physiological self-recognition with another setup that avoided severing connections by manipulating root interactions between closely connected ramets, between remotely connected ramets and between disconnected ramets within one genet. We found that ramets grown with disconnected neighbors had less new stolon mass, similar root mass but higher root allocation as compared to ramets grown with connected neighbors. There was no difference in ramet growth between closely connected- and remotely connected-neighbor treatments. We suggest that severing connections affects ramet interactions by disrupting their physiological integration. Using the second setup, we provide unbiased evidence for physiological self-recognition, while also suggesting that it can persist over long distances

Can bryophyte groups increase functional resolution in tundra ecosystems?

Funding Information: This study was supported by a grant to SL from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie, Grant No. 797446 and by the Independent Research Fund Denmark, Grant no. 0135-00140B. Funding from the Academy of Finland (grant 322266), National Science Foundation (1504224, 1836839, PLR-1504381 and PLR-1836898), Independent Research Fund Denmark (9040-00314B), Moscow State University, (project No 121032500089-1), Natural Sciences and Engineering Research Council of Canada, ArcticNet, Polar Continental Shelf Program, Northern Science Training Program, Polar Knowledge Canada, Royal Canadian Mounted Police, Tomsk State University competitiveness improvement program and the Russian Science Foundation (grant No 20-67-46018) are gratefully acknowledged. Matthias Ahrens provided valuable insights on the cushion growth form, and we are most thankful. We thank Gaius Shaver and two anonymous reviewers for providing valuable critique and input to earlier versions of this manuscript. Publisher Copyright: © the author(s) or their institution(s).The relative contribution of bryophytes to plant diversity, primary productivity, and ecosystem functioning increases towards colder climates. Bryophytes respond to environmental changes at the species level, but because bryophyte species are relatively difficult to identify, they are often lumped into one functional group. Consequently, bryophyte function remains poorly resolved. Here, we explore how higher resolution of bryophyte functional diversity can be encouraged and implemented in tundra ecological studies. We briefly review previous bryophyte functional classifications and the roles of bryophytes in tundra ecosystems and their susceptibility to environmental change. Based on shoot morphology and colony organization, we then propose twelve easily distinguishable bryophyte functional groups. To illustrate how bryophyte functional groups can help elucidate variation in bryophyte effects and responses, we compiled existing data on water holding capacity, a key bryophyte trait. Although plant functional groups can mask potentially high interspecific and intraspecific variability, we found better separation of bryophyte functional group means compared with previous grouping systems regarding water holding capacity. This suggests that our bryophyte functional groups truly represent variation in the functional roles of bryophytes in tundra ecosystems. Lastly, we provide recommendations to improve the monitoring of bryophyte community changes in tundra study sites.Peer reviewe

Is analysing the nitrogen use at the plant canopy level a matter of choosing the right optimization criterion?

Optimization theory in combination with canopy modeling is potentially a powerful tool for evaluating the adaptive significance of photosynthesis-related plant traits. Yet its successful application has been hampered by a lack of agreement on the appropriate optimization criterion. Here we review how models based on different types of optimization criteria have been used to analyze traits—particularly N reallocation and leaf area indices—that determine photosynthetic nitrogen-use efficiency at the canopy level. By far the most commonly used approach is static-plant simple optimization (SSO). Static-plant simple optimization makes two assumptions: (1) plant traits are considered to be optimal when they maximize whole-stand daily photosynthesis, ignoring competitive interactions between individuals; (2) it assumes static plants, ignoring canopy dynamics (production and loss of leaves, and the reallocation and uptake of nitrogen) and the respiration of nonphotosynthetic tissue. Recent studies have addressed either the former problem through the application of evolutionary game theory (EGT) or the latter by applying dynamic-plant simple optimization (DSO), and have made considerable progress in our understanding of plant photosynthetic traits. However, we argue that future model studies should focus on combining these two approaches. We also point out that field observations can fit predictions from two models based on very different optimization criteria. In order to enhance our understanding of the adaptive significance of photosynthesis-related plant traits, there is thus an urgent need for experiments that test underlying optimization criteria and competing hypotheses about underlying mechanisms of optimization

Can bryophyte groups increase functional resolution in tundra ecosystems?

The relative contribution of bryophytes to plant diversity, primary productivity, and ecosystem functioning increases towards colder climates. Bryophytes respond to environmental changes at the species level, but because bryophyte species are relatively difficult to identify, they are often lumped into one functional group. Consequently, bryophyte function remains poorly resolved. Here, we explore how higher resolution of bryophyte functional diversity can be encouraged and implemented in tundra ecological studies. We briefly review previous bryophyte functional classifications and the roles of bryophytes in tundra ecosystems and their susceptibility to environmental change. Based on shoot morphology and colony organization, we then propose twelve easily distinguishable bryophyte functional groups. To illustrate how bryophyte functional groups can help elucidate variation in bryophyte effects and responses, we compiled existing data on water holding capacity, a key bryophyte trait. Although plant functional groups can mask potentially high interspecific and intraspecific variability, we found better separation of bryophyte functional group means compared with previous grouping systems regarding water holding capacity. This suggests that our bryophyte functional groups truly represent variation in the functional roles of bryophytes in tundra ecosystems. Lastly, we provide recommendations to improve the monitoring of bryophyte community changes in tundra study sites

Review of Botanical excursions on the northern Sørøya (Finnmark, Norway), edited by N. Koroleva, E. Borovichev, A. Melekhin and Tom E. Ness

No abstract available!(Published: 10 October 2014)Citation: Polar Research 2014, 33, 26018, http://dx.doi.org/10.3402/polar.v33.2601

Review of Botanical excursions on the northern Sørøya (Finnmark, Norway)

No abstract available! (Published: 10 October 2014) Citation: Polar Research 2014, 33, 26018, http://dx.doi.org/10.3402/polar.v33.2601

Effects of simulated shade on growth, number of branches and biomass in Hylocomium splendens and Racomitrium lanuginosum

The effects of simulated shade in terms of reduced light quantity (PPFD) and changed red:far-red ratio (R:FR ratio) on the growth in length, in number of branches and in biomass, were examined in a greenhouse experiment with Hylocomium splendens and Racomitrium lanuginosum, two species from habitats with different light conditions (with H. splendens often in more shaded microsites). Using ten intact moss turfs per species which had been collected on Iceland at 4 m distance between replicate turfs, we tested, whether light quantity affected growth and biomass, whether changes in light quantity and red:far-red ratio affected the number of branches, and whether the two species differed in these responses. Reduced light quantity (i.e. PPFD level) caused a greater length increase, decreased biomass, and biomass:length ratio in both species, but the magnitude of response varied greatly between species. Furthermore, in R. lanuginosum spectral shade (i.e. reduced PPFD and a lower R:FR ratio) generally caused stronger responses than neutral shade, with only a reduction in PPFD. H. splendens (from the shaded habitat) responded less strongly to the shade treatments than R. lanuginosum (from the open habitat) did. In addition to these effects of shading, there were strong effects of the turf of origin in both species, and in many cases the interaction between turf of origin and shading treatment was significant as well

Biomechanical properties of the terrestrial mosses Pleurozium schreberi (Brid.) Mitt. and Pogonatum japonicum Sull. &amp; Lesq. along altitudinal gradients in northern Japan

Altitudinal gradients along mountain slopes provide valuable opportunities to study variation in plant traits in response to changes in environmental conditions along such  gradients. This study focused on biomechanical traits of two moss species, the more or less horizontally growing Pleurozium schreberi and the erect-growing Pogonatum japonicum, along altitudinal gradients on two mountains in Hokkaido, northern Japan. We measured stem diameter in two directions to determine the second moment of area I, used three-point bending tests with free stem ends to determine the slope of the force-deflection curve dF/dx, and used these data to calculate Young’s modulus and flexural rigidity of the stems. Both species showed much variation in all traits among replicates in the samples at each altitude. Environmental variation associated with altitude had more effect on the biomechanical traits of P. japonicum than on those of P. schreberi. Stems of P. japonicum were thicker (larger I) than those of P. schreberi and had a larger Young’s modulus and flexural rigidity. Stems tended to become thinner (lower second moment of area) and less rigid (lower flexural rigidity) at increasing altitude in both species