A Recessive Mutation Resulting in a Disabling Amino Acid Substitution (T194R) in the LHX3 Homeodomain Causes Combined Pituitary Hormone Deficiency

Background/Aims: Recessive mutations in the LHX3 homeodomain transcription factor gene are associated with developmental disorders affecting the pituitary and nervous system. We describe pediatric patients with combined pituitary hormone deficiency (CPHD) who harbor a novel mutation in LHX3. Methods: Two female siblings from related parents were examined. Both patients had neonatal complications. The index patient had CPHD featuring deficiencies of GH, LH, FSH, PRL, and TSH, with later onset of ACTH deficiency. She also had a hypoplastic anterior pituitary, respiratory distress, hearing impairment, and limited neck rotation. The LHX3 gene was sequenced and the biochemical properties of the predicted altered proteins were characterized. Results: A novel homozygous mutation predicted to change amino acid 194 from threonine to arginine (T194R) was detected in both patients. This amino acid is conserved in the DNA-binding homeodomain. Computer modeling predicted that the T194R change would alter the homeodomain structure. The T194R protein did not bind tested LHX3 DNA recognition sites and did not activate the a-glycoprotein and PRL target genes. Conclusion: The T194R mutation affects a critical residue in the LHX3 protein. This study extends our understanding of the phenotypic features, molecular mechanism, and developmental course associated with mutations in the LHX3 gene. copyright (C) 2012 S. Karger AG, Base

Definição conceitual e operacional das características definidoras do diagnóstico de enfermagem Padrão de Sono Prejudicado

Objetivo: apresentar o conhecimento produzido sobre sono e Síndrome Coronariana Aguda a fim de auxiliar na elaboração das definições operacionais e conceituais das características definidoras do diagnóstico de enfermagem Padrão de Sono Prejudicado (00198). Método: revisão integrativa nas bases de dados COCHRANE; SCOPUS; MEDLINE (Medical Literature Analysis and Retrieval System Online) via Pubmed; LILACS (Latin American and Caribean Health Science Literature Database); CINAHL (Cumulative Index to Nursing and Allied Health Literature) e EMBASE (The Excerpta Medica Database). Ao final das buscas, 2827 estudos identificados, 43 selecionados para leitura e 10 incluídos. A literatura cinzenta também foi incluída. Resultados: importantes achados relacionados a evidências clínicas e fatores contribuintes do sono foram encontrados na revisão realizada. Porém, para a construção das definições das características definidoras, fez-se necessário lançar mão de literatura cinzenta, como um dicionário da Língua Portuguesa e dois livros-texto sobre o sono. Conclusão: tais definições poderão auxiliar os enfermeiros da prática na coleta de informações, na determinação do referido diagnóstico de enfermagem e, assim, no direcionamento de cuidados ao que tange à quantidade e qualidade do sono de pacientes internados com Síndrome Coronariana Aguda. Também auxiliarão nas próximas etapas do processo de validação desse diagnóstico à população referida.Objetivo: presentar el conocimiento producido sobre sueño y Síndrome Coronario Agudo a fin de auxiliar en la elaboración de las definiciones operacionales y conceptuales de las características definidoras del diagnóstico de enfermería Estándar de Sueño Perjudicado (00198). Método: revisión integradora en las bases de datos COCHRANE; SCOPUS; MEDLINE (Medical Literature Analysis and Retrieval System Online) via Pubmed; LILACS (Latin American and Caribean Health Science Literature Database); CINAHL (Cumulative Index to Nursing and Allied Health Literature) e EMBASE (The Excerpta Medica Database). Al final de las búsquedas, 2827 estudios identificados, 43 seleccionados para lectura y 10 incluidos. La literatura gris también fue incluida. Resultados: importantes hallados relacionados a las evidencias clínicas y factores contribuyentes del sueño fueron encontrados en la revisión realizada. Sin embargo, para la construcción de las definiciones de las características definidoras es necesario usar la literatura gris, como un diccionario de la Lengua Portuguesa y dos libros texto sobre el sueño. Conclusión: tales definiciones podrán auxiliar a los enfermeros de la práctica en la recolección de informaciones, en la determinación del referido diagnóstico de enfermería y así, en el direccionamiento de cuidados sobre la cantidad y calidad del sueño de pacientes internados con Síndrome Coronario Agudo. También auxiliarán en las próximas etapas del proceso de validez de ese diagnóstico a la población referida.Objective: to present the knowledge produced about sleep and Acute Coronary Syndrome in order to assist in the elaboration of the operational and conceptual definitions of the defining characteristics of the nursing diagnosis Disturbed Sleep Pattern (00198). Method: integrative review in the following databases: COCHRANE; SCOPUS; MEDLINE (Medical Literature Analysis and Retrieval System Online) via Pubmed; LILACS (Latin American and Caribbean Health Science Literature Database); CINAHL (Cumulative Index to Nursing and Allied Health Literature) and EMBASE (The Excerpta Medical Database). At the end of the search, 2827 studies were found, 43 were selected for reading, and 10 were included in the review. The gray literature was also included. Results: important findings related to clinical evidence and contributing factors of sleep were found in the review. However, in order to build definitions of the defining characteristics, it was necessary to use gray literature, such as a Portuguese dictionary and two textbooks about sleep. Conclusion: the definitions will help nurses in their practice in the collection of information, in the determination of the nursing diagnosis studied here, and in directing care measures with respect to quantity and quality of sleep of Acute Coronary Syndrome inpatients. They will also assist in the next steps of the validation of this diagnosis to the referred population

Informe técnico anual: POA 1996-97

De acuerdo con los compromisos contraidos con PROFRIJOL según el POA 1996-97, en México se realizaron un total de 12 actividades en 4 subproyectos, correspondientes a los proyectos de Mejoramiento Genético de Frijol Mesoamericano y Desarrollo del Cultivo de Frijol en Sistemas de Frijol Sostenibles. Los trabajos se llevaron a cabo en las áreas de influencia de los campos experimentales del INIFAP correspondientes a Valle de México, estado de México; Cotaxtla e Isla, Veracruz y Tapachula, Chiapas. En el subproyecto de Mejoramiento de Frijol de Grano Negro, donde se realizó el mayor número de actividades, destacaron los siguientes resultados: a) identificación de nuevos padres con resistencia múltiple a enfermedades (G 1640, G 19833, MAM 48 y MAR 3) y alta resistencia a picudo de la vaina (J-l 17, Hidalgo 58 y México 332); b) evaluación y selección de familias para baja fertilidad en Isla, Veracruz y por resistencia a picudo del ejote en Santa Lucía, estado de México; c) identificación de líneas de alto rendimiento en los ensayos ECAR, tales como DOR 453, ICTA Ostúa, DOR 454 e ICTA Santa Gertrudis; d) la selección por los productores en evaluaciones participativas de las líneas DOR 500 y 11-307- 5E. En el subproyecto de tolerancia a baja fertilidad, sobresalieron las líneas G 5150, II-429-IE, G 5141, G 23276 y Negro Tacaná; para fijación de nitrógeno fueron buenas SEQ 12, TLP 22, BAT 1467 y NAG 161.En el subproyecto de Desarrollo del Cultivo en Sistemas de Producción Sostenibles, en prácticas de manejo y conservación de suelos desarrolladas en la región de Los Tuxtlas, en las terrazas de muro vivo con un año de establecimiento no hubo acumulación de suelo, pero sí en las de tres años, donde se registró una ganancia promedio de 5.9 cm de suelo. Por lo que concierne al efecto de coberturas (Canavalia sp.y Stizolobim sp.), éstas incrementaron significativamente los rendimientos del frijol. Finalmente en producción de semilla básica, se cumplió con el compromiso de producir por lo menos 2 toneladas de las variedades Negro Tacaná y Negro Cotaxtla 91; en este mismo rubro se produjeron adicionalmente más de 4 toneladas de semilla, bajo el programa nacional de “Kilo por Kilo”.Instituto de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP)Programa Cooperativo Regional de Frijol para Centroamérica, México y el Caribe (PROFRIJOL)UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Estación Experimental Agrícola Fabio Baudrit Moreno (EEAFBM

Sublethal exposure of small few-layer graphene promotes metabolic alterations in human skin cells

Small few-layer graphene (sFLG), a novel small-sized graphene-related material (GRM), can be considered as an intermediate degradation product of graphene. GRMs have a promising present and future in the field of biomedicine. However, safety issues must be carefully addressed to facilitate their implementation. In the work described here, the effect of sub-lethal doses of sFLG on the biology of human HaCaT keratinocytes was examined. A one-week treatment of HaCaTs with sub-lethal doses of sFLG resulted in metabolome remodeling, dampening of the mitochondrial function and a shift in the redox state to pro-oxidant conditions. sFLG raises reactive oxygen species and calcium from 24 hours to one week after the treatment and this involves the activation of NADPH oxidase 1. Likewise, sFLG seems to induce a shift from oxidative phosphorylation to glycolysis and promotes the use of glutamine as an alternative source of energy. When sub-toxic sFLG exposure was sustained for 30 days, an increase in cell proliferation and mitochondrial damage were observed. Further research is required to unveil the safety of GRMs and degradation-derived products before their use in the workplace and in practical applications

Analyzing the perceptions of pre-service teachers about the modules integración laboral v and vi during the practicum experience

Tesis (Profesor de Inglés para la Enseñanza Básica y Media, Licenciado en Educación)Este estudio tiene como objetivo analizar y entender las percepciones de los estudiantes practicantes de pedagogía en Inglés de la Universidad Nacional Andrés Bello (UNAB) sobre los contenidos teóricos cubiertos en los módulos de Integración Laboral V y VI. Adicionalmente, este estudio también tiene la intención de identificar las percepciones de los practicantes de pedagogía en Inglés respecto al contenido enseñado en los módulos previamente mencionados y determinar si los contenidos enseñados en estos módulos son practicados antes de su implementación en la práctica. Finalmente, este estudio tiene la intención de determinar la percepción de los practicantes de pedagogía en inglés respecto a la reflexión realizada en estos módulos. Con el fin de lograr estos objetivos, dos grupos de enfoque fueron conducidos, la transcripción de estos fue analizada y codificada a través de un análisis temático. Los principales hallazgos de esta investigación fueron que los contenidos eran considerados repetitivos y no contextualizados a la realidad del país y que los practicantes sentían que hay una necesidad de haber tenido más instancias prácticas así también como de ocasiones reflexivas.This study aims to analyse and understand the perceptions of Second Language Teaching Education (SLTE) pre-service teachers from Universidad Nacional Andres Bello (UNAB) about the theoretical contents covered in the modules Integración Laboral V and VI. Additionally, this study also intends to identify the perceptions of SLTE pre-service teachers regarding the content taught in the previously mentioned modules, to determine (whether) the contents taught in these modules are practiced before its implementation in the practicum. Finally, this study also intends to determine the perception of SLTE pre-service teachers regarding the reflection done in these modules. In order to achieve these objectives, two focus groups were conducted, and the transcriptions of these focus groups were analysed and coded through thematic analysis. The main findings of this research were that the content was considered to be repetitive and not contextualized to the country’s reality, and that pre-service teachers felt that there is a need for more practical instances as well as for reflective occasions

Planeamiento estratégico de Transportes Leon del Sur 2019 - 2023

La presente tesis ha desarrollado el Planeamiento Estratégico para Transportes León del Sur en función al Modelo Secuencial del Proceso Estratégico propuesto por D’Alessio (2016). Transportes León del Sur es una empresa que nace en la ciudad de Arequipa en 1985 y que actualmente brinda el servicio de transporte interprovincial de pasajeros y el servicio de envío de encomiendas. La empresa opera en 16 rutas a nivel nacional y centra sus operaciones en el sur del país. Se ha propuesto como visión para la empresa que para 2023 esta será una de las diez principales empresas de transporte terrestre de pasajeros en el Perú en nivel de ventas, actuando de manera socialmente responsable, generando rentabilidad para los accionistas buscando la satisfacción del cliente y manteniendo un compromiso con la seguridad, respetando las normativas legales que contribuyen al crecimiento del Perú. Asimismo, la misión propuesta para Transportes León del Sur es que la empresa busca conectar a las personas con sus destinos alrededor del territorio nacional; trabajando responsablemente por la calidad de su servicio y la seguridad, cuidando el impacto en la comunidad y el medio empleando una flota moderna con tecnología ecoeficiente, operando siempre comprometidos con el crecimiento del país y el bienestar de todos nuestros stakeholders; siendo la diferenciación ante sus competidores su principal ventaja competitiva. Finalmente, como resultado del Planeamiento Estratégico para Transportes León del Sur, se plantearon cuatro objetivos a largo plazo, se formularon 21 estrategias y se diseñaron 15 objetivos de corto plazo, los cuales han sido plasmados en el Tablero de Control Balanceado, herramienta que permite medir el logro de dichos objetivos.This thesis has developed the Strategic Planning for Transportes León del Sur based on the Sequential Model of the Strategic Process proposed by D’Alessio (2016). Transportes León del Sur is a company that was born in Arequipa in 1985 and currently provides the interprovincial passenger transport service and the parcel delivery service. The company operates on 16 routes nationwide and focuses its operations in the south of Peru. It has been proposed as a vision for the company that by 2023 this will be one of the ten main passenger land transport companies in Peru in terms of sales, acting in a socially responsible manner, generating profitability for shareholders seeking customer satisfaction and maintaining a commitment to security, respecting the legal regulations that contribute to the growth of Peru. Likewise, the proposed mission for Transportes León del Sur is that the company seeks to connect people with their destinations around the national territory; working responsibly for the quality of its service and security, taking care of the impact on the community and the environment using a modern fleet with eco-efficient technology, always operating committed to the country's growth and the well-being of all our stakeholders; being the differentiation before its competitors, its main competitive advantage. Finally, as a result of the Strategic Planning for Transportation León del Sur, four long-term objectives were raised, 21 strategies were formulated and 15 short-term objectives were designed, which have been embodied in the Balanced Score Card, a tool that allows measuring the achievement of these objectives.Tesi

Violacein treatment modulates acute and chronic inflammation through the suppression of cytokine production and induction of regulatory T cells

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOInflammation is a necessary process to control infection. However, exacerbated inflammation, acute or chronic, promotes deleterious effects in the organism. Violacein (viola), a quorum sensing metabolite from the Gram-negative bacterium Chromobacterium violaceum, has been shown to protect mice from malaria and to have beneficial effects on tumors. However, it is not known whether this drug possesses anti-inflammatory activity. In this study, we investigated whether viola administration is able to reduce acute and chronic autoimmune inflammation. For that purpose, C57BL/6 mice were intraperitoneally injected with 1 mu g of LPS and were treated with viola (3.5mg/kg) via i.p. at the same time-point. Three hours later, the levels of inflammatory cytokines in the sera and phenotypical characterization of leukocytes were determined. Mice treated with viola presented a significant reduction in the production of inflammatory cytokines compared with untreated mice. Interestingly, although viola is a compound derived from bacteria, it did not induce inflammation upon administration to naive mice. To test whether viola would protect mice from an autoimmune inflammation, Experimental Autoimmune Encephalomyelitis (EAE)-inflicted mice were given viola i.p. at disease onset, at the 10th day from immunization. Viola-treated mice developed mild EAE disease in contrast with placebo-treated mice. The frequencies of dendritic cells and macrophages were unaltered in EAE mice treated with viola. However, the sole administration of viola augmented the levels of splenic regulatory T cells (CD4+ Foxp3+). We also found that adoptive transfer of viola-elicited regulatory T cells significantly reduced EAE. Our study shows, for the first time, that violacein is able to modulate acute and chronic inflammation. Amelioration relied in suppression of cytokine production (in acute inflammation) and stimulation of regulatory T cells (in chronic inflammation). New studies must be conducted in order to assess the possible use of viola in therapeutic approaches in human autoimmune diseases.Inflammation is a necessary process to control infection. However, exacerbated inflammation, acute or chronic, promotes deleterious effects in the organism. Violacein (viola), a quorum sensing metabolite from the Gram-negative bacteriumChromobacterium vio105116FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP [2011/17965-3]CNPq [471066/2012-5]FAPESP [2014/02631-0, 2011/23664-6, 2012/01892-0]2011/17965-3; 471066/2012-5; 2014/02631-0; 2011/23664-6; 2012/01892-0sem informaçã

A Treatment with a Protease Inhibitor Recombinant from the Cattle Tick (Rhipicephalus Boophilus microplus) Ameliorates Emphysema in Mice

Aims: To determine whether a serine protease inhibitor treatment can prevent or minimize emphysema in mice.Methods: C57BL/6 mice were subjected to porcine pancreatic elastase (PPE) nasal instillation to induce emphysema and were treated with a serine protease inhibitor (rBmTI-A) before (Protocol 1) and after (Protocol 2) emphysema development. in both protocols, we evaluated lung function to evaluate the airway resistance (Raw), tissue damping (Gtis) and tissue elastance (Htis). the inflammatory profile was analyzed in the bronchoalveolar lavage (BALF) and through the use of morphometry; we measured the mean linear intercept (Lm) (to verify alveolar enlargement), the volume proportion of collagen and elastic fibers, and the numbers of macrophages and metalloprotease 12 (MMP-12) positive cells in the parenchyma. We showed that at both time points, even after the emphysema was established, the rBmTI-A treatment was sufficient to reverse the loss of elastic recoil measured by Htis, the alveolar enlargement and the increase in the total number of cells in the BALF, with a primary decrease in the number of macrophages. Although, the treatment did not control the increase in macrophages in the lung parenchyma, it was sufficient to decrease the number of positive cells for MMP-12 and reduce the volume of collagen fibers, which was increased in PPE groups. These findings attest to the importance of MMP-12 in PPE-induced emphysema and suggest that this metalloprotease could be an effective therapeutic target.Laboratorios de Investigacao Medica do Hospital das Clinicas da Faculdade de Medicina da USP (LIM/HC)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ São Paulo, Dept Med, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biol Sci, São Paulo, BrazilUNIFESP EPM, Dept Bioquim, São Paulo, BrazilUFABC, Ctr Ciencias Nat & Humanas, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biol Sci, São Paulo, BrazilUNIFESP EPM, Dept Bioquim, São Paulo, BrazilWeb of Scienc

ELABORACIÓN Y VALIDACIÓN DE INSTRUMENTOS DE ASISTENCIA DE ENFERMERÍA PARA PACIENTES EN UNIDAD DE TERAPIA INTENSIVA

Objetivo: elaborar e validar instrumento para assistência de enfermagem, baseado em literatura específica para pacientes internados em Unidade de Terapia Intensiva, de um hospital universitário do estado de São Paulo. Método: estudo metodológico, com coleta de dados de agosto a dezembro de 2015, em três fases: caracterização da população e levantamento dos diagnósticos de enfermagem, elaboração, e validação do instrumento para o registro do Processo de Enfermagem. Para a validação de conteúdo, utilizou-se a técnica Delphi, com um grupo de especialistas na área, composto por 11 jurados. Resultados: durante a coleta de dados, 152 pacientes estiveram internados, 61,18% do sexo masculino, com idade média de 54,9 anos. O conteúdo do instrumento foi validado com índice de validação > 0,81. Conclusão: a construção de instrumentos para o processo de Enfermagem é útil para facilitar a implementação da assistência, evidenciar a assistência, incrementar a comunicação e segurança dos cuidados em saúde.Objective: to develop and validate an instrument for nursing care, based on specific literature for patients hospitalized in the Intensive Care Unit of a university hospital in the state of São Paulo.Method: methodological study, with data collection from August to December 2015, in three phases: characterization of the population and survey of the nursing diagnoses, development, and validation of the instrument for the registration of the Nursing Process.The Delphi technique was used for the content validation, with a group of experts in the area, composed of 11 judges.Results: during the data collection, 152 patients were hospitalized, 61.18% male, with a mean age of 54.9 years.The content of the instrument was validated with a validation index >0.81. Conclusion: the construction of instruments for the Nursing Process is useful to facilitate the implementation of care, to evidence the care and to increase communication and the safety of the healthcare.Objetivo: elaborar y validar instrumento para asistencia de enfermería, con base en la literatura específica para pacientes ingresados en Unidad de Terapia Intensiva de un hospital universitario del estado de São Paulo. Método: estudio metodológico, con recogida de datos de agosto a diciembre de 2015, en tres etapas: caracterización de la población y recogida de los diagnósticos de enfermería, elaboración, y validación del instrumento para el registro del Proceso de Enfermería. Para la validación de contenido, se utilizó la técnica Delphi, con un grupo de especialistas en el área, compuesto por 11 jurados. Resultados: durante la obtención de datos, 152 pacientes estuvieron ingresados, 61,18% del sexo masculino, con edad media de 54,9 años. Se evaluó el contenido del instrumento por medio de índice de validación > 0,81. Conclusión: la construcción de instrumentos para el proceso de Enfermería es útil para facilitar la implementación de la asistencia, evidenciarla, así como para incrementar la comunicación y seguridad de los cuidados en salud