Regulatory roles of the cadherin superfamily

Charged with the task of providing a molecular link between adjacent cells, the cadherin superfamily consists of over 100 members and populates the genomes of organisms ranging from vertebrates to cniderians. This breadth hints at what decades of research has confirmed: that cadherin-based adhesion and signaling events regulate diverse cellular processes including cell-sorting, differentiation, cell survival, proliferation, cell polarity, and cytoskeletal organization

Fatigue and Structural Analysis of Azimuth Thruster Assembly

Composite is stated as constituent of two or more materials which retain their own physical and chemical property during the time of application, but produce a component which inherent the properties of its constituent materials and makes it better for the real time USAge. There are varieties of processing techniques for fabricating composite parts or structures such as: (1) Resin Transfer Moulding, (2) Pultrusion, (3) Filament Winding, (4) Autoclave Moulding. Among all these technique of exercising composite materials, the filament winding technique is the most appropriate because it avails the user with the ease of USAge, as well as gives wide range of degree of freedom for fabricating or manufacturing objects. In the paper we basically reveal the maximum approach made to study basic theory related to the filament winding technique or method, which provides initial platform for the new learner

Mapping 123 million neonatal, infant and child deaths between 2000 and 2017

Since 2000, many countries have achieved considerable success in improving child survival, but localized progress remains unclear. To inform efforts towards United Nations Sustainable Development Goal 3.2—to end preventable child deaths by 2030—we need consistently estimated data at the subnational level regarding child mortality rates and trends. Here we quantified, for the period 2000–2017, the subnational variation in mortality rates and number of deaths of neonates, infants and children under 5 years of age within 99 low- and middle-income countries using a geostatistical survival model. We estimated that 32% of children under 5 in these countries lived in districts that had attained rates of 25 or fewer child deaths per 1,000 live births by 2017, and that 58% of child deaths between 2000 and 2017 in these countries could have been averted in the absence of geographical inequality. This study enables the identification of high-mortality clusters, patterns of progress and geographical inequalities to inform appropriate investments and implementations that will help to improve the health of all populations

The induced protein CoiA promotes recombination during genetic transformation in Streptococcus pneumoniae.

The induced protein CoiA promotes recombination during genetic transformation in Streptococcus pneumoniae

An Unstable Competence-Induced Protein, CoiA, Promotes Processing of Donor DNA after Uptake during Genetic Transformation in Streptococcus pneumoniae

Natural genetic transformation in Streptococcus pneumoniae entails transcriptional activation of at least two sets of genes. One set of genes, activated by the competence-specific response regulator ComE, is involved in initiating competence, whereas a second set is activated by the competence-specific alternative sigma factor ComX and functions in DNA uptake and recombination. Here we report an initial characterization of CoiA, a ComX-dependent gene product that is induced during competence and is required for transformation. CoiA is widely conserved among gram-positive bacteria, and in streptococci, the entire coiA locus composed of four genes is conserved. By use of immunoblot assay, we show that, similar to its message, CoiA protein is transient, appearing at 10 min and largely disappearing by 30 min post-competence induction. Using complementation analysis, we establish that coiA is the only gene of this induced locus needed for transformability. We find no indication of CoiA having a role in regulating competence. Finally, using (32)P- and (3)H-labeled donor DNA, we demonstrate that a coiA mutant can internalize normal amounts of donor DNA compared to the wild-type strain but is unable to process it into viable transformants, suggesting a role for CoiA after DNA uptake, either in DNA processing or recombination

Choline Starvation Induces the Gene licD2 in Streptococcus pneumoniae

Mutant strains of Streptococcus pneumoniae were constructed to monitor the regulation of three dispersed genes known or predicted to act in choline metabolism. One gene (licD2) was regulated in response to choline deprivation over a 30-fold range. The other two (SP1860 and licC) responded little if at all to the same challenge