A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes

Contains fulltext : 117166.pdf (publisher's version ) (Open Access)Synthetic glucocorticoids are potent anti-inflammatory drugs but show dose-dependent metabolic side effects such as the development of insulin resistance and obesity. The precise mechanisms involved in these glucocorticoid-induced side effects, and especially the participation of adipose tissue in this are not completely understood. We used a combination of transcriptomics, antibody arrays and bioinformatics approaches to characterize prednisolone-induced alterations in gene expression and adipokine secretion, which could underlie metabolic dysfunction in 3T3-L1 adipocytes. Several pathways, including cytokine signalling, Akt signalling, and Wnt signalling were found to be regulated at multiple levels, showing that these processes are targeted by prednisolone. These results suggest that mechanisms by which prednisolone induce insulin resistance include dysregulation of wnt signalling and immune response processes. These pathways may provide interesting targets for the development of improved glucocorticoids

Prednisolone-induced changes in gene-expression profiles in healthy volunteers.

Item does not contain fulltextBACKGROUND: Prednisolone and other glucocorticoids (GCs) are potent anti-inflammatory and immunosuppressive drugs. However, prolonged use at a medium or high dose is hampered by side effects of which the metabolic side effects are most evident. Relatively little is known about their effect on gene-expression in vivo, the effect on cell subpopulations and the relation to the efficacy and side effects of GCs. AIM: To identify and compare prednisolone-induced gene signatures in CD4 T lymphocytes and CD14 monocytes derived from healthy volunteers and to link these signatures to underlying biological pathways involved in metabolic adverse effects. MATERIALS & METHODS: Whole-genome expression profiling was performed on CD4 T lymphocytes and CD14 monocytes derived from healthy volunteers treated with prednisolone. Text-mining analyses was used to link genes to pathways involved in metabolic adverse events. RESULTS: Induction of gene-expression was much stronger in CD4 T lymphocytes than in CD14 monocytes with respect to fold changes, but the number of truly cell-specific genes where a strong prednisolone effect in one cell type was accompanied by a total lack of prednisolone effect in the other cell type, was relatively low. Subsequently, a large set of genes was identified with a strong link to metabolic processes, for some of which the association with GCs is novel. CONCLUSION: The identified gene signatures provide new starting points for further study into GC-induced transcriptional regulation in vivo and the mechanisms underlying GC-mediated metabolic side effects.1 juli 201

The Personalized Acne Treatment Tool — Recommendations to facilitate a patient-centered approach to acne management from the Personalizing Acne: Consensus of ExpertsCapsule Summary

Background: Acne, a commonly treated skin disease, requires patient-centered management due to its varying presentations, chronicity, and impact on health-related quality of life. Despite this, evidence-based clinical guidelines focus primarily on clinical severity of facial acne, omitting important patient- and disease-related factors, including ongoing management. Objectives: To generate recommendations to support patient-centered acne management, which incorporate priority and prognostic factors beyond conventional clinical severity, traditionally defined by grading the appearance and extent of visible lesions. Methods: The Personalizing Acne: Consensus of Experts consisted of 17 dermatologists who used a modified Delphi approach to reach consensus on statements regarding patient- and treatment-related factors pertaining to patient-centered acne management. Consensus was defined as ≥75% voting “agree” or “strongly agree.” Results: Recommendations based on factors such as acne sequelae, location of acne, high burden of disease, and individual patient features were generated and incorporated into the Personalized Acne Treatment Tool. Limitations: Recommendations are based on expert opinion, which may differ from patients’ perspectives. Regional variations in healthcare systems may not be represented. Conclusions: The Personalizing Acne: Consensus of Experts panel provided practical recommendations to facilitate individualized management of acne, based on patient features, which can be implemented to improve treatment outcomes, adherence, and patient satisfaction

Chalés paulistanos

This article studies the origins, flourishment and decadence of a certain style of\ud building, known as swiss cottages or chalets, in the urban environment of Sao Paulo. Its source\ud is found in 19th century romanticism and in the last quarter of the eighteen hundreds this kind\ud of construction was very popular. At this time it had taken on a high symbolic value although\ud of an ambiguous nature - for it was both intimately associated to an idealization of country\ud life and to technological modernity, which was then being ushered in the city of Sao Paulo. It\ud became very popular on account of the availability of industrialized construction material for\ud importation and it expanded during a construction boom that attained the city of Sao Paulo,\ud after 1875. On the downfall of Brazilian monarchy, it gradually disappeared, as it became\ud the object of restrictive measures from the local county, which considered its proliferation unruly.\ud From the beginning of the XXth century, there was a change in public taste and urban reforms\ud were implemented in downtown Sao Paulo (1902 to 1914), which was followed by a desire\ud for a new urban scene, more in touch with the system of values and interests of hegemonic\ud sectors of the local political elite

Measurement of the Mass of the Z-Boson and the Energy Calibration of Lep

Contains fulltext : 26847___.PDF (publisher's version ) (Open Access