К разработке технологии возведения геокомпозитных охранных систем горных выработок

У статті розглянуто відмінності використання особливо тонко дисперсних в’яжучих (ОТДВ) у підземних умовах для створення елементів геокомпозитних конструкцій.In article the differences of the use especially thinly of dispersible astringent are considered in underground terms for creation elements of geocomposit constructions

Big data Empowered Logistics Services Platform

Logistics section is one of the most important industrial sections to contribute to European economy. To improving efficiency and energy efficient of logistics, European Commission call new research theme ‘smart, green and integrated transport’ in its H2020 program. The paper presents a version on providing a cloud based platform for supporting big data empowered logistics services to respond this call. The research is supported by inter-disciplinary approaches, which brings experts from telecommunication, cloud computing, sensor networking, service-oriented computing, data analysis, transportation, and logistics areas to work together to provide real-world solutions for future logistics. The research questions and challenges of the platform are highlighted. Overall architecture and data collection are presented

Transcriptomics reveal an integrative role for maternal thyroid hormones during zebrafish embryogenesis

Thyroid hormones (THs) are essential for embryonic brain development but the genetic mechanisms involved in the action of maternal THs (MTHs) are still largely unknown. As the basis for understanding the underlying genetic mechanisms of MTHs regulation we used an established zebrafish monocarboxylic acid transporter 8 (MCT8) knock-down model and characterised the transcriptome in 25hpf zebrafish embryos. Subsequent mapping of differentially expressed genes using Reactome pathway analysis together with in situ expression analysis and immunohistochemistry revealed the genetic networks and cells under MTHs regulation during zebrafish embryogenesis. We found 4,343 differentially expressed genes and the Reactome pathway analysis revealed that TH is involved in 1681 of these pathways. MTHs regulated the expression of core developmental pathways, such as NOTCH and WNT in a cell specific context. The cellular distribution of neural MTH-target genes demonstrated their cell specific action on neural stem cells and differentiated neuron classes. Taken together our data show that MTHs have a role in zebrafish neurogenesis and suggest they may be involved in cross talk between key pathways in neural development. Given that the observed MCT8 zebrafish knockdown phenotype resembles the symptoms in human patients with Allan-Herndon-Dudley syndrome our data open a window into understanding the genetics of this human congenital condition.Portuguese Fundacao para Ciencia e Tecnologia (FCT) [PTDC/EXPL/MARBIO/0430/2013]; CCMAR FCT Plurianual financing [UID/Multi/04326/2013]; FCT [SFRH/BD/111226/2015, SFRH/BD/108842/2015, SFRH/BPD/89889/2012]; FCT-IF Starting Grant [IF/01274/2014]info:eu-repo/semantics/publishedVersio

Double Dielectric Barrier (DBD) plasma-assisted deposition of chemical stabilized nanoparticles on polyamide 6,6 and polyester fabrics

The development of new multifunctional textiles containing nanoparticles (NPs) has had a special interest in several applications for pharmaceutical, medical, engineering, agricultural, and food products.[1-2] Cu, Zn and especially Ag NPs exhibit strong antibacterial activities on a broad spectrum of bacteria.[3-5] Most of the antimicrobial textiles coated with NPs are not able to perform a controlled release of the antibiotic species. Thus, the immobilization of NPs in the substrate or its inclusion in polymeric matrix is essential to control the NPs antibiotic effect with time. Dielectric barrier discharge (DBD) plasma technology is one of the most effective non-thermal plasma sources.[6] However, an even dispersion and coating of NPs onto fabrics remain a challenge due to the high degree of aggregation of metal NPs.[7] Some capping agents were described to increase the suspension stability such as citrate and SDS.[8] In this work, Ag, Zn, and Cu NPs deposition on DBD plasma pre-treated polyamide 6,6 (PA66) and polyester (PES) were tested for the production of durable antibacterial textiles. SEM-EDX analysis and the effect of some NPs stabilizers (e.g. sodium citrate, sodium alginate and Polyvinyl alcohol (PVA)) was analysed by dynamic light scattering (DLS) in term of size, polydispersity index and zeta potential. XPS analyses prove the DBD efficacy in providing oxygen species onto the fabric’s surfaces. The SEM analyses prove the deposition of the Ag and Cu NPs onto the PES and PA66 fabrics. No zinc was detected. However, antimicrobial tests in PES shows that all the NPs have an antimicrobial effect but Cu and Zn show activity only in S. aureus and Ag only in E.coli. Cu shows a reasonable dispersion onto the fibres but PVP coated AgNPs display a high level of aggregation even after 1 hour of ultrasonic treatment. To solve instability and aggregation problems, NPs suspensions were prepared in different concentrations (1, 2.5 and 5 wt%) of citrate, alginate and PVA using water and ethanol as control by ultrasonic bath. In table 1 are resumed the best results obtained for each NP compared to water as control. Ethanol and PVA were disregarded due to the highest instability and lowest ζ potential, respectively. XPS, SEM and antimicrobial data shows lack in coating uniformity. It is clear that doesn't exist a univocal dispersant and concentration for all NPs. Despite the improving in ζ potentials and stability of the colloids, the obtained sizes still show a high degree of aggregation.info:eu-repo/semantics/publishedVersio

Resilience Analysis of Service Oriented Collaboration Process Management systems

Collaborative business process management allows for the automated coordination of processes involving human and computer actors. In modern economies it is increasingly needed for this coordination to be not only within organizations but also to cross organizational boundaries. The dependence on the performance of other organizations should however be limited, and the control over the own processes is required from a competitiveness perspective. The main objective of this work is to propose an evaluation model for measuring a resilience of a Service Oriented Architecture (SOA) collaborative process management system. In this paper, we have proposed resilience analysis perspectives of SOA collaborative process systems, i.e. overall system perspective, individual process model perspective, individual process instance perspective, service perspective, and resource perspective. A collaborative incident and maintenance notification process system is reviewed for illustrating our resilience analysis. This research contributes to extend SOA collaborative business process management systems with resilience support, not only looking at quantification and identification of resilience factors, but also considering ways of improving the resilience of SOA collaborative process systems through measures at design and run-time

EU H2020 MSCA RISE Project FIRST - “virtual Factories: Interoperation suppoRting buSiness innovation”

FIRST – “virtual Factories: Interoperation suppoRting buSiness innovation”, is a European H2020 project, founded by the RESEARCH AND INNOVATION STAFF EXCHANGE (RISE) Work Programme as part of the Marie Skłodowska-Curie actions. The project concerns with Manufacturing 2.0 and aims at providing the new technology and methodology to describe manufacturing assets; to compose and integrate the existing services into collaborative virtual manufacturing processes; and to deal with evolution of changes. This Chapter provides an overview of the state of the art for the research topics related to the project research objectives, and then it presents the progresses the project achieved up to now towards the implementation of the proposed innovations

Monocyte mitochondrial dysfunction, inflammaging, and inflammatory pyroptosis in major depression

BACKGROUND: The macrophage theory of depression states that macrophages play an important role in Major Depressive Disorder (MDD). METHODS: MDD patients (N = 140) and healthy controls (N = 120) participated in a cross-sectional study investigating the expression of apoptosis/growth and lipid/cholesterol pathway genes (BAX, BCL10, EGR1, EGR2, HB-EGF, NR1H3, ABCA1, ABCG1, MVK, CD163, HMOX1) in monocytes (macrophage/microglia precursors). Gene expressions were correlated to a set of previously determined and reported inflammation-regulating genes and analyzed with respect to various clinical parameters. RESULTS: MDD monocytes showed an overexpression of the apoptosis/growth/cholesterol and the TNF genes forming an inter-correlating gene cluster (cluster 3) separate from the previously described inflammation-related gene clusters (containing IL1 and IL6). While upregulation of monocyte gene cluster 3 was a hallmark of monocytes of all MDD patients, upregulation of the inflammation-related clusters was confirmed to be found only in the monocytes of patients with childhood adversity. The latter group also showed a downregulation of the cholesterol metabolism gene MVK, which is known to play an important role in trained immunity and proneness to inflammation. CONCLUSIONS: The upregulation of cluster 3 genes in monocytes of all MDD patients suggests a premature aging of the cells, i.e. mitochondrial apoptotic dysfunction and TNF "inflammaging", as a general feature of MDD. The overexpression of the IL-1/IL-6 containing inflammation clusters and the downregulation of MVK in monocytes of patients with childhood adversity indicates a shift in this condition to a more severe inflammation form (pyroptosis) of the cells, additional to the signs of premature aging and inflammaging

Clinical and preclinical therapeutic outcome metrics for USH2A-related disease

USH2A variants are the most common cause of Usher syndrome type 2, characterized by congenital sensorineural hearing loss and retinitis pigmentosa (RP), and also contribute to autosomal recessive non-syndromic RP. Several treatment strategies are under development; however, sensitive clinical trial endpoint metrics to determine therapeutic efficacy have not been identified. In the present study, we have performed longitudinal retrospective examination of the retinal and auditory symptoms in (i) 56 biallelic molecularly confirmed USH2A patients and (ii) ush2a mutant zebrafish to identify metrics for the evaluation of future clinical trials and rapid preclinical screening studies. The patient cohort showed a statistically significant correlation between age and both rate of constriction for the ellipsoid zone length and hyperautofluorescent outer retinal ring area. Visual acuity and pure tone audiograms are not suitable outcome measures. Retinal examination of the novel ush2au507 zebrafish mutant revealed a slowly progressive degeneration of predominantly rods, accompanied by rhodopsin and blue cone opsin mislocalization from 6 to 12 months of age with lysosome-like structures observed in the photoreceptors. This was further evaluated in the ush2armc zebrafish model, which revealed similar changes in photopigment mislocalization with elevated autophagy levels at 6 days post fertilization, indicating a more severe genotype-phenotype correlation and providing evidence of new insights into the pathophysiology underlying USH2A-retinal disease

Interoperability framework of virtual factory and business innovation

Interoperability framework of virtual factory and business innovationTask T51 Design a common schema and schema evolution framework for supporting interoperabilityTask T52 Design interoperability framework for supporting datainformation transformation service composition and business process cooperation among partnersA draft version is envisioned for month 44 which will be updated to reflect incremental changes driven by the other working packages for month 72 deliverable 7.

Intestinal Ralstonia pickettii augments glucose intolerance in obesity

An altered intestinal microbiota composition has been implicated in the pathogenesis of metabolic disease including obesity and type 2 diabetes mellitus (T2DM). Low grade inflammation, potentially initiated by the intestinal microbiota, has been suggested to be a driving force in the development of insulin resistance in obesity. Here, we report that bacterial DNA is present in mesenteric adipose tissue of obese but otherwise healthy human subjects. Pyrosequencing of bacterial 16S rRNA genes revealed that DNA from the Gram-negative species Ralstonia was most prevalent. Interestingly, fecal abundance of Ralstonia pickettii was increased in obese subjects with pre-diabetes and T2DM. To assess if R. pickettii was causally involved in development of obesity and T2DM, we performed a proof-of-concept study in diet-induced obese (DIO) mice. Compared to vehicle-treated control mice, R. pickettii-treated DIO mice had reduced glucose tolerance. In addition, circulating levels of endotoxin were increased in R. pickettii-treated mice. In conclusion, this study suggests that intestinal Ralstonia is increased in obese human subjects with T2DM and reciprocally worsens glucose tolerance in DIO mice.Peer reviewe