1,356 research outputs found
Temperature and relative humidity requirements for conidiogenesis of Beauveria bassiana (Deuteromycetes: Moniliaceae).
Assays were conducted to assess the number of Beauveria bassiana (Bals) Vuill. conidia on Diatraea saccharalis F. (Lepidoptera: Pyralidae), Nezera viridula (L.) and Piezodorus guildinii (Westwood) (Hemiptera: Pantatormidae) corpses maintained at different levels of relative humidity (RH) (75%, 80%, 90% and 100%) and temperatures (22oC, 26oC, 30oC and 34oC) during five days. The isolates produced conidia when exposed to RH from 75% to 100%. Conidiogenesis was incipient at 75% RH on D.saccharalis larvae, but did not occur on N. viridula and P. guildinii. In ideal conditions od RH and during 10 days, mathematical equations were developed to estimate the number of condia produced by isolates ARSEF 933 and ARSEF 2515. Conidia number were shown to be dependant on RH, temperature, fungal isolate, host species, host stage, and time
Incorporating oldman saltbush hay and prickly pear in diets for red Sindhi calves
This study was designed to evaluate the nutrient intake, digestibility, degradability, and ruminal characteristics of Sindhi heifers fed diets that contained a combined total of 75% oldman saltbush hay (hereafter saltbush hay) and prickly pear cactus. Eight 12-month old intact male red Sindhi calves (four fistulated and four non-fistulated) with an initial mean weight of 170 ± 5 kg were assigned to 4 × 4 Latin squares, where factors consisted of four diets, namely 15% hay and 60% cactus; 30% hay and 45% cactus; 45% hay and 30% cactus; and 60% hay and 15% cactus, and four times at which rumen fluid was collected. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) intakes in kg/day and NDF in percentage live weight (LW), water intake, salinity, and conductivity increased with hay level. Intake and digestibility of non-fibrous carbohydrates were curvilinear with higher values in diets containing 30% saltbush hay. The apparent digestibility of dry matter (DM) and organic matter decreased linearly as the concentration of saltbush hay increased in the diet. The pH of the rumen fluid was within the acceptable range for favourable microbial growth. The low temperature and high salinity and conductivity indices in the diets should be viewed with caution at higher concentrations of saltbush hay, because of a possible decrease in nutrient absorption and the development of health problems in the animals. Apparent degradability coefficients of DM and NDF were affected significantly by inclusion of prickly pear and saltbush hay in the diets.Keywords: animal nutrition, apparent digestibility, Atriplex nummularia Lindl, Opuntia ficus, ruminal degradation, semi-arid environmen
Atomic Dark Matter
We propose that dark matter is dominantly comprised of atomic bound states.
We build a simple model and map the parameter space that results in the early
universe formation of hydrogen-like dark atoms. We find that atomic dark matter
has interesting implications for cosmology as well as direct detection:
Protohalo formation can be suppressed below for weak scale dark matter due to Ion-Radiation interactions in the
dark sector. Moreover, weak-scale dark atoms can accommodate hyperfine
splittings of order 100 \kev, consistent with the inelastic dark matter
interpretation of the DAMA data while naturally evading direct detection
bounds.Comment: 17 pages, 3 figure
Inverse flux quantum periodicity of magnetoresistance oscillations in two-dimensional short-period surface superlattices
Transport properties of the two-dimensional electron gas (2DEG) are
considered in the presence of a perpendicular magnetic field and of a {\it
weak} two-dimensional (2D) periodic potential modulation in the 2DEG plane. The
symmetry of the latter is rectangular or hexagonal. The well-known solution of
the corresponding tight-binding equation shows that each Landau level splits
into several subbands when a rational number of flux quanta pierces the
unit cell and that the corresponding gaps are exponentially small. Assuming the
latter are closed due to disorder gives analytical wave functions and
simplifies considerably the evaluation of the magnetoresistivity tensor
. The relative phase of the oscillations in and
depends on the modulation periods involved. For a 2D modulation
with a {\bf short} period nm, in addition to the Weiss oscillations
the collisional contribution to the conductivity and consequently the tensor
show {\it prominent peaks when one flux quantum passes
through an integral number of unit cells} in good agreement with recent
experiments. For periods nm long used in early experiments, these
peaks occur at fields 10-25 times smaller than those of the Weiss oscillations
and are not resolved
Extended gaussian ensemble solution and tricritical points of a system with long-range interactions
The gaussian ensemble and its extended version theoretically play the
important role of interpolating ensembles between the microcanonical and the
canonical ensembles. Here, the thermodynamic properties yielded by the extended
gaussian ensemble (EGE) for the Blume-Capel (BC) model with infinite-range
interactions are analyzed. This model presents different predictions for the
first-order phase transition line according to the microcanonical and canonical
ensembles. From the EGE approach, we explicitly work out the analytical
microcanonical solution. Moreover, the general EGE solution allows one to
illustrate in details how the stable microcanonical states are continuously
recovered as the gaussian parameter is increased. We found out that it
is not necessary to take the theoretically expected limit
to recover the microcanonical states in the region between the canonical and
microcanonical tricritical points of the phase diagram. By analyzing the
entropy as a function of the magnetization we realize the existence of
unaccessible magnetic states as the energy is lowered, leading to a treaking of
ergodicity.Comment: 8 pages, 5 eps figures. Title modified, sections rewritten,
tricritical point calculations added. To appear in EPJ
Lycopene-rich extract from red guava ( Psidium guajava L.) displays cytotoxic effect against human breast adenocarcinoma cell line MCF-7 via an apoptotic-like pathway
This study investigated a lycopene-rich extract from red guava (LEG) for its chemical composition using spectrophotometry, mass spectrometry, attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), and computational studies. The cytotoxic activity of LEG and the underlying mechanism was studied in human breast adenocarcinoma cells (MCF-7), murine fibroblast cells (NIH-3T3), BALB/c murine peritoneal macrophages, and sheep blood erythrocytes by evaluating the cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and flow cytometry. Spectrophotometry analysis showed that LEG contained 20% of lycopene per extract dry weight. Experimental and theoretical ATR-FTIR suggests the presence of lycopene, whereas MS/MS spectra obtained after fragmentation of the molecular ion [M]+• of 536.4364 show fragment ions at m/z 269.2259, 375.3034, 444.3788, and 467.3658, corroborating the presence of lycopene mostly related to all-trans configuration. Treatment with LEG (1600 to 6.25μg/mL) for 24 and 72h significantly affected the viability of MCF-7 cells (mean half maximal inhibitory concentration [IC50]=29.85 and 5.964μg/mL, respectively) but not NIH-3T3 cells (IC50=1579 and 911.5μg/mL, respectively). Furthermore LEG at concentrations from 800 to 6.25μg/mL presented low cytotoxicity against BALB/c peritoneal macrophages (IC50≥800μg/mL) and no hemolytic activity. LEG (400 and 800μg/mL) caused reduction in the cell proliferation and induced cell cycle arrest, DNA fragmentation, modifications in the mitochondrial membrane potential, and morphologic changes related to granularity and size in MCF-7 cells; however, it failed to cause any significant damage to the cell membrane or display necrosis or traditional apoptosis. In conclusion, LEG was able to induce cytostatic and cytotoxic effects on breast cancer cells probably via induction of an apoptotic-like pathway.The authors acknowledge the computational time provided by CENAPAD/SP on the project proj697. Alexandra Plácido is gratefully to FCT by her grant SFRH/BD/97995/2013, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. The work at REQUIMTE/LAQV received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT) through project UID/QUI/50006/2013. Adriany das G. N. Amorim is grateful to CAPES by for the doctoral fellowship process no. 99999.004236/2014-09 in Federal University of Piauí (UFPI). Eder A. Barbosa is grateful to PNPD/CAPES for its post-doctoral fellowship.info:eu-repo/semantics/publishedVersio
The Large Magellanic Cloud and the Distance Scale
The Magellanic Clouds, especially the Large Magellanic Cloud, are places
where multiple distance indicators can be compared with each other in a
straight-forward manner at considerable precision. We here review the distances
derived from Cepheids, Red Variables, RR Lyraes, Red Clump Stars and Eclipsing
Binaries, and show that the results from these distance indicators generally
agree to within their errors, and the distance modulus to the Large Magellanic
Cloud appears to be defined to 3% with a mean value of 18.48 mag, corresponding
to 49.7 Kpc. The utility of the Magellanic Clouds in constructing and testing
the distance scale will remain as we move into the era of Gaia.Comment: 23 pages, accepted for publication in Astrophysics and Space Science.
From a presentation at the conference The Fundamental Cosmic Distance Scale:
State of the Art and the Gaia Perspective, Naples, May 201
Assessing phage therapy against Pseudomonas aeruginosa using a Galleria mellonella infection model
The Galleria mellonella infection model was used to assess the in vivo efficacy of phage therapy against laboratory and clinical strains of Pseudomonas aeruginosa. In a first series of experiments, Galleria were infected with the laboratory strain P. aeruginosa PAO1 and were treated with varying multiplicity of infection (MOI) of phages either 2 h post-infection (treatment) or 2 h pre-infection (prevention) via injection into the haemolymph. To address the kinetics of infection, larvae were bled over a period of 24 h for quantification of bacteria and phages. Survival rates at 24 h when infected with 10 cells/larvae were greater in the prevention versus treatment model (47% vs. 40%, MOI = 10; 47% vs. 20%, MOI = 1; and 33% vs. 7%, MOI = 0.1). This pattern held true when 100 cells/larvae were used (87% vs. 20%, MOI = 10; 53% vs. 13%, MOI = 1; 67% vs. 7%, MOI = 0.1). By 24 h post-infection, phages kept bacterial cell numbers in the haemolymph 1000-fold lower than in the non-treated group. In a second series of experiments using clinical strains to further validate the prevention model, phages protected Galleria when infected with both a bacteraemia (0% vs. 85%) and a cystic fibrosis (80% vs. 100%) isolate. Therefore, this study validates the use of G. mellonella as a simple, robust and cost-effective model for initial in vivo examination of P. aeruginosa-targeted phage therapy, which may be applied to other pathogens with similarly low infective doses
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