187 research outputs found

    A Mathematical Model of Liver Cell Aggregation In Vitro

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    The behavior of mammalian cells within three-dimensional structures is an area of intense biological research and underpins the efforts of tissue engineers to regenerate human tissues for clinical applications. In the particular case of hepatocytes (liver cells), the formation of spheroidal multicellular aggregates has been shown to improve cell viability and functionality compared to traditional monolayer culture techniques. We propose a simple mathematical model for the early stages of this aggregation process, when cell clusters form on the surface of the extracellular matrix (ECM) layer on which they are seeded. We focus on interactions between the cells and the viscoelastic ECM substrate. Governing equations for the cells, culture medium, and ECM are derived using the principles of mass and momentum balance. The model is then reduced to a system of four partial differential equations, which are investigated analytically and numerically. The model predicts that provided cells are seeded at a suitable density, aggregates with clearly defined boundaries and a spatially uniform cell density on the interior will form. While the mechanical properties of the ECM do not appear to have a significant effect, strong cell-ECM interactions can inhibit, or possibly prevent, the formation of aggregates. The paper concludes with a discussion of our key findings and suggestions for future work

    The geochemistry of environmentally important trace elements in UK coals, with special reference to the Parkgate coal in the Yorkshire-Nottinghamshire Coalfield, UK

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    The Parkgate coal of Langsettian age in the Yorkshire-Nottinghamshire coalfield is typical of many coals in the UK in that it has a high sulphur (S) content. Detailed information on the distribution of the forms of S, both laterally and vertically through the seam, was known from previous investigations. In the present work, 38 interval samples from five measured sections of the coal were comprehensively analysed for major, minor and trace elements and the significance of the relationships established using both raw and centered log transformed data. The major elements are used to quantify the variations in the inorganic and organic coal components and determine the trace element associations. Pyrite contains nearly all of the Hg, As, Se, Tl and Pb and is also the major source of the Mo, Ni, Cd and Sb. The clays contain the following elements in decreasing order of association: Rb, Cs, Li, Ga, U, Cr, V, Sc, Y, Bi, Cu, Nb, Sn, Te and Th. Nearly all of the Rb is present in the clay fraction, whereas for elements such as V, Cu and U, a significant amount is thought to be present in the organic matter, based on the K vs trace element regression equations. Only Ge, and possibly Be, would appear to have a dominant organic source. The trace element concentrations are calculated for pyrite, the clay fraction and organic matter. For pyrite it is noted that concentrations agree with published data from the Yorkshire-Nottinghamshire coalfield and also that Tl concentrations (median of 0.33 ppm) in the pyrite are greater than either Hg or Cd. Unlike these elements, Tl has attracted less attention and possibly more information is needed on its anthropogenic distribution and impacts on man and the environment. A seawater source is thought to be responsible for the high concentrations of S, Cl and the non-detrital trace elements in the Parkgate coal. Indicative of the seawater control is the Th/U ratio, which expresses the detrital to non-detrital element contributions. Using other elements, similar ratios can be calculated, which in combination offer greater interpretative value. (C) 2009 Elsevier B.V. All rights reserved

    A Statistical Study of Galactic SNRs using the PMN Survey

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    The Parkes-MIT-NRAO (PMN) radio survey has been used to generate a quasi all-sky study of Galactic Supernova Remnants (SNRs) at a common frequency of 4.85 GHz. We present flux densities estimated for the sample of 110 Southern Galactic SNRs (up to Dec = - 65 deg.) observed with the Parkes 64-m radio telescope and an additional sample of 54 from the Northern PMN (up to Dec = +64 deg.) survey undertaken with the Green Bank 43-m (20 SNRs) and 91-m (34 SNRs) radio telescopes. Out of this total sample of 164 selected SNRs (representing 71% of the 231 known SNRs in the Green catalogue) we consider 138 to provide reliable estimates of flux density and surface brightness distribution. This sub-sample represents those SNRs which fall within carefully chosen selection criteria which minimises the effects of the known problems in establishing reliable fluxes from the PMN survey data. Our selection criteria are based on a judicious restriction of source angular size and telescope beam together with careful evaluation of fluxes on a case by case basis. This gives confidence in the newly derived PMN fluxes when the selection criteria are respected. We find a sharp drop off in the flux densities for Galactic SNRs beyond 4 Jy and then a fairly flat distribution from 5-9 Jy, a slight decline and a further flat distribution from 9-20 Jy though the numbers of SNR in each Jy bin are low. We also re-visit the contentious Sigma-D relation to determine a new power law index for a sub-sample of shell type SNRs which yields beta= -2.2 +/- 0.6. This new evaluation of the Sigma-D relation, applied to the restricted sample, provides new distance estimates and their Galactic scale height distribution. We find a peak in the SNR distribution between 7-11 kpc with most restricted to +/- 100 pc Galactic scale height.Comment: 14 pages, 7 figures. Accepted for publishing in Astrophysics and Space Scienc

    On thermodynamically consistent Stefan problems with variable surface energy

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    A thermodynamically consistent two-phase Stefan problem with temperature-dependent surface tension and with or without kinetic undercooling is studied. It is shown that these problems generate local semiflows in well-defined state manifolds. If a solution does not exhibit singularities, it is proved that it exists globally in time and converges towards an equilibrium of the problem. In addition, stability and instability of equilibria is studied. In particular, it is shown that multiple spheres of the same radius are unstable if surface heat capacity is small; however, if kinetic undercooling is absent, they are stable if surface heat capacity is sufficiently large.Comment: To appear in Arch. Ration. Mech. Anal. The final publication is available at Springer via http://dx.doi.org/10.1007/s00205-015-0938-y. arXiv admin note: substantial text overlap with arXiv:1101.376

    The velocity potential and the interacting force for two spheres moving perpendicularly to the line joining their centers

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    The velocity potential around two spheres moving perpendicularly to the line joining their centers is given by a series of spherical harmonics. The appropriateness of the truncation is evaluated by determining the residual normal surface velocity on the spheres. In evaluating the residual normal velocity, a recursive procedure is constructed to evaluate the spherical harmonics to reduce computational effort and truncation error as compared to direct transformation or numerical integration. We estimate the lift force coefficient for touching spheres to be 0.577771, compared to the most accurate earlier estimate of 0.51435 by Miloh (1977).Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42701/1/10665_2004_Article_BF00127479.pd

    Protecting the environment through insect farming as a means to produce protein for use as livestock, poultry, and aquaculture feed

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    Securing protein for the approximate 10 billion humans expected to inhabit our planet by 2050 is a major priority for the global community. Evidence has accrued over the past 30 years that strongly supports and justifies the sustainable use of insects as a means to produce protein products as feed for pets, livestock, poultry, and aquacultured species. Researchers and entrepreneurs affiliated with universities and industries, respectively, from 18 nations distributed across North and South America, Europe, Asia, Africa and Australia contributed to the development of this article, which is an indication of the global interest on this topic. A brief overview of insects as feed for the aquaculture industry along with a review of the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae), as a model for such systems is provided
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