An Abundance Analysis for Five Red Horizontal Branch Stars in the Extremely Metal Rich Globular Cluster NGC 6553

We provide a high dispersion line-by-line abundance analysis of five red HB stars in the extremely metal rich galactic globular cluster NGC 6553. These red HB stars are significantly hotter than the very cool stars near the tip of the giant branch in such a metal rich globular cluster and hence their spectra are much more amenable to an abundance analysis than would be the case for red giants. We find that the mean [Fe/H] for NGC 6553 is -0.16 dex, comparable to the mean abundance in the galactic bulge found by McWilliam & Rich (1994) and considerably higher than that obtained from an analysis of two red giants in this cluster by Barbuy etal (1999). The relative abundance for the best determined alpha process element (Ca) indicates an excess of alpha process elements of about a factor of two. The metallicity of NGC 6553 reaches the average of the Galactic bulge and of the solar neighborhood.Comment: 29 pages, 6 figures, accepted for publication in the Ap

NGC 2419 -- Another Remnant of Accretion by the Milky Way

We isolate a sample of 43 upper RGB stars in the extreme outer halo Galactic globular cluster NGC 2419 from two Keck/DEIMOS slitmasks. The probability that there is more than one contaminating halo field star in this sample is extremely low. Analysis of moderate resolution spectra of these cluster members, as well as of our Keck/HIRES high resolution spectra of a subsample of them, demonstrates that there is a small but real spread in Ca abundance of ~ 0.2 dex within this massive metal-poor globular cluster. This provides additional support to earlier suggestions that NGC 2419 is the remnant of a dwarf galaxy accreted long ago by the Milky Way.Comment: Accepted to the Astrophysical Journa

Multiwavelength observations of the M15 intermediate velocity cloud

We present Westerbork Synthesis Radio Telescope HI images, Lovell Telescope multibeam HI wide-field mapping, Wisconsin H-alpha Mapper facility images, William Herschel Telescope longslit echelle CaII observations, and IRAS ISSA 60 and 100 micron coadded images towards the intermediate velocity cloud located in the general direction of the M15 globular cluster. When combined with previously-published Arecibo data, the HI gas in the IVC is found to be clumpy, with peak HI column density of 1.5x10^(20) cm^(-2), inferred volume density (assuming spherical symmetry) of 24 cm^(-3)/(D kpc), and maximum brightness temperature at a resolution of 81x14 arcsec of 14 K. The HI gas in the cloud is warm, with a minimum FWHM value of 5 km/s, corresponding to a kinetic temperature, in the absence of turbulence, of 540 K. There are indications in the HI data of 2-component velocity structure in the IVC, indicative of cloudlets. This velocity structure is also tentatively seen in the CaK spectra, although the SNR is low. The main IVC condensation is detected by WHAM in H-alpha with intensities uncorrected for Galactic absorption of upto 1.3 Rayleigh, indicating that the cloud is partially ionised. The FWHM of the ionised component, at a resolution of 1 degree, exceeds 30 km/s. The spatial and velocity coincidence of the H-alpha and HI peaks in emission is qualitatively good. Finally, the 100 and 60 micron IRAS images show spatial coincidence over a 0.7 degree field, with low and intermediate-velocity gas, respectively, indicating that the IVC may contain dust.Comment: MNRAS, in pres

Structural and Functional Similarity between the Bacterial Type III Secretion System Needle Protein PrgI and the Eukaryotic Apoptosis Bcl-2 Proteins

Background: Functional similarity is challenging to identify when global sequence and structure similarity is low. Activesites or functionally relevant regions are evolutionarily more stable relative to the remainder of a protein structure and provide an alternative means to identify potential functional similarity between proteins. We recently developed the FASTNMR methodology to discover biochemical functions or functional hypotheses of proteins of unknown function by experimentally identifying ligand binding sites. FAST-NMR utilizes our CPASS software and database to assign a function based on a similarity in the structure and sequence of ligand binding sites between proteins of known and unknown function. Methodology/Principal Findings: The PrgI protein from Salmonella typhimurium forms the needle complex in the type III secretion system (T3SS). A FAST-NMR screen identified a similarity between the ligand binding sites of PrgI and the Bcl-2 apoptosis protein Bcl-xL. These ligand binding sites correlate with known protein-protein binding interfaces required for oligomerization. Both proteins form membrane pores through this oligomerization to release effector proteins to stimulate cell death. Structural analysis indicates an overlap between the PrgI structure and the pore forming motif of Bcl-xL. A sequence alignment indicates conservation between the PrgI and Bcl-xL ligand binding sites and pore formation regions. This active-site similarity was then used to verify that chelerythrine, a known Bcl-xL inhibitor, also binds PrgI. Conclusions/Significance: A structural and functional relationship between the bacterial T3SS and eukaryotic apoptosis was identified using our FAST-NMR ligand affinity screen in combination with a bioinformatic analysis based on our CPASS program. A similarity between PrgI and Bcl-xL is not readily apparent using traditional global sequence and structure analysis, but was only identified because of conservation in ligand binding sites. These results demonstrate the unique opportunity that ligand-binding sites provide for the identification of functional relationships when global sequence and structural information is limited

Dust and the type II-Plateau supernova 2004dj

We present mid-infrared (MIR) spectroscopy of a Type II-plateau supernova, SN 2004dj, obtained with the Spitzer Space Telescope, spanning 106--1393 d after explosion. MIR photometry plus optical/near-IR observations are also reported. An early-time MIR excess is attributed to emission from non-silicate dust formed within a cool dense shell (CDS). Most of the CDS dust condensed between 50 d and 165 d, reaching a mass of 0.3 x 10^{-5} Msun. Throughout the observations much of the longer wavelength (>10 microns) part of the continuum is explained as an IR echo from interstellar dust. The MIR excess strengthened at later times. We show that this was due to thermal emission from warm, non-silicate dust formed in the ejecta. Using optical/near-IR line-profiles and the MIR continua, we show that the dust was distributed as a disk whose radius appeared to be slowly shrinking. The disk radius may correspond to a grain destruction zone caused by a reverse shock which also heated the dust. The dust-disk lay nearly face-on, had high opacities in the optical/near-IR regions, but remained optically thin in the MIR over much of the period studied. Assuming a uniform dust density, the ejecta dust mass by 996 d was 0.5 +/- 0.1) x 10^{-4} Msun, and exceeded 10^{-4}Msun by 1393 d. For a dust density rising toward the center the limit is higher. Nevertheless, this study suggests that the amount of freshly-synthesized dust in the SN 2004dj ejecta is consistent with that found from previous studies, and adds further weight to the claim that such events could not have been major contributors to the cosmic dust budget.Comment: ApJ in press; minor changes c.f. v

ROPS: A New Search for Habitable Earths in the Southern Sky

We present the first results from our Red Optical Planet Survey (ROPS) to search for low mass planets orbiting late type dwarfs (M5.5V - M9V) in their habitable zones (HZ). Our observations, with the red arm of the MIKE spectrograph (0.5 - 0.9 microns) at the 6.5 m Magellan Clay telescope at Las Campanas Observatory indicate that >= 92 per cent of the flux lies beyond 0.7 microns. We use a novel approach that is essentially a hybrid of the simultaneous iodine and ThAr methods for determining precision radial velocities. We apply least squares deconvolution to obtain a single high S/N ratio stellar line for each spectrum and cross correlate against the simultaneously observed telluric line profile, which we derive in the same way. Utilising the 0.62 - 0.90 micron region, we have achieved an r.m.s. precision of 10 m/s for an M5.5V spectral type star with spectral S/N ~160 on 5 minute timescales. By M8V spectral type, a precision of ~30 m/s at S/N = 25 is suggested, although more observations are needed. An assessment of our errors and scatter in the radial velocity points hints at the presence of stellar radial velocity variations. Of our sample of 7 stars, 2 show radial velocity signals at 6-sigma and 10-sigma of the cross correlation uncertainties. If the signals are planetary in origin, our findings are consistent with estimates of Neptune mass planets that predict a frequency of 13 - 27 per cent for early M dwarfs.Our current analysis indicates the we can achieve a sensitivity that is equivalent to the amplitude induced by a 6 M_Earth planet orbiting in the habitable zone. Based on simulations, we estimate that <10 M_Earth habitable zone planets will be detected in a new stellar mass regime, with <=20 epochs of observations.Comment: MNRAS accepted: 14 pages, 8 figures, 3 table

Spatio-Temporal Characteristics of Global Warming in the Tibetan Plateau during the Last 50 Years Based on a Generalised Temperature Zone - Elevation Model

Temperature is one of the primary factors influencing the climate and ecosystem, and examining its change and fluctuation could elucidate the formation of novel climate patterns and trends. In this study, we constructed a generalised temperature zone elevation model (GTEM) to assess the trends of climate change and temporal-spatial differences in the Tibetan Plateau (TP) using the annual and monthly mean temperatures from 1961-2010 at 144 meteorological stations in and near the TP. The results showed the following: (1) The TP has undergone robust warming over the study period, and the warming rate was 0.318°C/decade. The warming has accelerated during recent decades, especially in the last 20 years, and the warming has been most significant in the winter months, followed by the spring, autumn and summer seasons. (2) Spatially, the zones that became significantly smaller were the temperature zones of -6°C and -4°C, and these have decreased 499.44 and 454.26 thousand sq km from 1961 to 2010 at average rates of 25.1% and 11.7%, respectively, over every 5-year interval. These quickly shrinking zones were located in the northwestern and central TP. (3) The elevation dependency of climate warming existed in the TP during 1961-2010, but this tendency has gradually been weakening due to more rapid warming at lower elevations than in the middle and upper elevations of the TP during 1991-2010. The higher regions and some low altitude valleys of the TP were the most significantly warming regions under the same categorizing criteria. Experimental evidence shows that the GTEM is an effective method to analyse climate changes in high altitude mountainous regions

Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and proinflammatory dysregulation

Pigs are evidently more resistant to avian than swine influenza A viruses, mediated in part through frontline epithelial cells and alveolar macrophages (AM). Although porcine AM (PAM) are crucial in influenza virus control, their mode of control is unclear. To gain insight into the possible role of PAM in the mediation of avian influenza virus resistance, we compared the host effects and replication of two avian (H2N3 and H6N1) and three mammalian (swine H1N1, human H1N1 and pandemic H1N1) influenza viruses in PAM. We found that PAM were readily susceptible to initial infection with all five avian and mammalian influenza viruses but only avian viruses caused early and extensive apoptosis (by 6 h of infection) resulting in reduced virus progeny and moderated pro- inflammation. Full length viral PB1-F2 present only in avian influenza viruses is a virulence factor that targets AM for mitochondrial associated apoptotic cell death. With the use of reverse genetics on an avian H5N1 virus, we found that full length PB1-F2 contributed to increased apoptosis and pro-inflammation but not to reduced virus replication. Taken together, we propose that early apoptosis of PAM limits the spread of avian influenza viruses and that PB1-F2 could play a contributory role in the process

Structure and in Vitro Transcription of Selected Human Transfer RNA Genes

The purpose of this study was to investigate human tRNA gene structure, organization, and expression by isolating and analyzing several human transfer RNA genes