Visualization of apoptotic cells using scanning acoustic microscopy and high frequency ultrasound

The goal of this project is to investigate changes in the acoustical properties of cells undergoing cell death for the development of a method for tissue apoptosis detection using high frequency ultrasound (10-60 MHz). A scanning acoustic microscope (SAM) was used for visualization of individual cells undergoing apoptosis (SASAM, Fraunhofer IBMT, Germany). The use of the SAM offers high resolution (1 m spot size) and therefore enables the exploration of acoustical properties of the cell nucleus. Cells were labeled with H33342 and DIOC 3(5) for visualizing condensed chromatin and membranes in fluorescence microscopy. In addition the same cell lines interrogated microscopically were investigated using high frequency ultrasound Recorded radio frequency (rf) data were analyzed using ultrasound spectroscopy. Integrated backscatter coefficients and attenuation values were computed for two cell lines: HeLa and MDCK. Both cell lines responded to the applied chemotherapeutic age nt by apoptosis, assessed by fluorescence microscopy. Acoustical and optical microscopy using the SASAM system clearly enabled a differentiation between apoptotic cells and cells not responding to the treatment. Apoptotic cells displayed a higher contrast in the acoustic images and were less regular in shape. Optical images of the same cells showed nuclear condensation and membrane disruption. Spectral parameters estimated from rf ultrasound showed a 100% increase in the integrated backscatter coefficients for HeLa and MDCK. Attenuation values were increased by 50% to 70% for both cell lines as a function of treatment time. The results of this investigation provide a better understanding of changes in the acoustical properties of cells with cell death and thus to the development of a non-invasive method for measuring the treatment response of tumors using acoustic waves

Lymphoepithelioma-like carcinoma of the breast: a diagnostic and therapeutic challenge

We present a patient with lymphoepithelioma-like carcinoma (lelc) of the breast whose diagnosis is illustrative of the pathology nuances that must be taken into account to successfully reach correct identification of the disease. We also present an overview of our patient’s proposed treatment in the context of 16 other reported lelc cases. Although lelc cases are rare, a sufficient number have been reported to discern the natural history of this pathologic entity and to undertake a review of those cases and of the application of oncologic first principles in their management. Given the potential for locoregional spread and distant metastases in lelc, adjuvant therapy has a role in the treatment of this entity

Caught in the middle: case study of a brachial (sentry) lymph node recurrence after resection and locoregional breast radiotherapy

To reduce local recurrence, adjuvant locoregional radiotherapy is given routinely for post-mastectomy breast patients with 4 or more positive lymph nodes. Most institutions adopt a 3- or 4-field radiotherapy technique, in which the field and shielding placements are informed by bony anatomic landmarks viewed on digitally reconstructed radiographs