Understanding Structure and Function in Semiarid Ecosystems: Implications for Terrestrial Carbon Dynamics in Drylands

This study advances understanding of how the changes in ecosystem structure and function associated with woody shrub encroachment in semi-arid grasslands alter ecosystem carbon (C) dynamics. In terms of both magnitude and dynamism, dryland ecosystems represent a major component of the global C cycle. Woody shrub encroachment is a widespread phenomenon globally, which is known to substantially alter ecosystem structure and function, with resultant impacts on C dynamics. A series of focal sites were studied at the Sevilleta National Wildlife Refuge in central New Mexico, USA. A space-for-time analogue was used to identify how landscape structure and function change at four stages over a grassland to shrubland transition. The research had three key threads: 1. Soil-associated carbon: Stocks of organic and inorganic C in the near-surface soil, and the redistribution of these C stocks by erosion during high-intensity rainfall events were quantified using hillslope-scale monitoring plots. Coarse (>2 mm) clasts were found to account for a substantial proportion of the organic and inorganic C in these calcareous soils, and the erosional effluxes of both inorganic and organic C increased substantially across the vegetation ecotone. Eroded sediment was found to be significantly enriched in organic C relative to the contributing soil with systematic changes in OC enrichment across the vegetation transition. The OC enrichment dynamics observed were inconsistent with existing understanding (derived largely from reductionist, laboratory-based experiments) that OC enrichment is largely insignificant in the erosional redistribution of C. 2. Plant biomass: Cutting-edge proximal remote sensing approaches, using a remotely piloted lightweight multirotor drone combined with structure-from-motion (SfM) photogrammetry were developed and used to quantify biomass carbon stocks at the focal field sites. In such spatially heterogeneous and temporally dynamic ecosystems existing measurement techniques (e.g. on-the-ground observations or satellite- or aircraft-based remote sensing) struggle to capture the complexity of fine-grained vegetation structure, which is crucial for accurately estimating biomass. The data products available from the novel SfM approach developed for this research quantified plants just 15 mm high, achieving a fidelity nearly two orders of magnitude finer than previous implementations of the method. The approach developed here will revolutionise the study of biomass dynamics in short-sward ecogeomorphic systems. 3. Ecohydrological modelling: Understanding the effects of water-mediated degradation processes on ecosystem carbon dynamics over greater than observable spatio-temporal scales is complicated by significant scale-dependencies and thus requires detailed mechanistic understanding. A process-based, spatially-explicit ecohydrological modelling approach (MAHLERAN - Model for Assessing Hillslope to Landscape Erosion, Runoff and Nutrients) was therefore comprehensively evaluated against a large assemblage of rainfall runoff events. This evaluation highlighted both areas of strength in the current model structure, and also areas of weakness for further development. The research has improved understanding of ecosystem degradation processes in semi-arid rangelands, and demonstrates that woody shrub encroachment may lead to a long-term reduction in ecosystem C storage, which is contrary to the widely promulgated view that woody shrub encroachment increases C storage in terrestrial ecosystems.NERC Doctoral Training Grant (NE/K500902/1)NSF Long Term Ecological Research Program at the Sevilleta National Wildlife Refuge (DEB-1232294

Origin of Properties in High Entropy Alloys

Abstract The novel class of alloys known as high entropy alloys (HEAs) present two fundamental problems; 1) prediction of their properties and reaction to alloying adjustments, 2) prediction of compositions capable of forming the random solid solution with simple crystal structure that appears to be key to their behaviour. Here DFT is applied to model the electronic structure of HEAs based on the CoCrFeNi pseudo base metal. This approach explains a number of properties such as preferred crystal structure and allows fundamental properties such as elastic moduli to be calculated accurately. The stability of HEAs is discussed and compared to that of bulk metallic glasses and a composition is produced which is capable of forming both a glassy and high entropy solid solution phase. A simple thermodynamic model is proposed to allow likely HEA solid solution forming compositions to be identified. This modelling approach using both DFT and thermodynamics is used to assess two potential high entropy alloys based on light metals. The approach shows that the electronic structure of HEAs may be used to predict their properties and therefore their behaviour is due to a free electron structure, it also suggests that the most important consideration in their stability as solid solution alloys is a lack of strong covalent interactions, ie a close to zero entropy of mixing

Comparison of bacterioneuston and bacterioplankton dynamics during a phytoplankton bloom in a fjord mesocosm

The bacterioneuston is the community of Bacteria present in surface microlayers, the thin surface film that forms the interface between aquatic environments and the atmosphere. In this study we compared bacterial cell abundance and bacterial community structure of the bacterioneuston and the bacterioplankton (from the subsurface water column) during a phytoplankton bloom mesocosm experiment. Bacterial cell abundance, determined by flow cytometry, followed a typical bacterioplankton response to a phytoplankton bloom, with Synechococcus and high nucleic acid (HNA) bacterial cell numbers initially falling, probably due to selective protist grazing. Subsequently HNA and low nucleic acid (LNA) bacterial cells increased in abundance but Synechococcus did not. There was no significant difference between bacterioneuston and bacterioplankton cell abundances during the experiment. Conversely, distinct and consistent differences between the bacterioneuston and the bacterioplankton community structure were observed. This was monitored simultaneously by Bacteria 16S rRNA gene terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). The conserved patterns of community structure observed in all of the mesocosms indicate that the bacterioneuston is distinctive and non-random

Abnormal systemic and ocular vascular response to temperature provocation in primary open-angle glaucoma patients:a case for autonomic failure?

PURPOSE. To assess systemic and ocular vascular reactivity in response to warm and cold provocation in untreated patients with primary open-angle glaucoma and normal control subjects. METHODS. Twenty-four patients with primary open-angle glaucoma and 22 normal control subjects were subjected to a modified cold pressor test involving immersion of the right hand in 40°C warm water followed by 4°C cold water exposure, and finger and ocular blood flow were assessed by means of peripheral laser Doppler flowmetry and retinal flowmetry, respectively. Finger and body temperature as well as intraocular pressure, systemic blood pressure, systemic pulse pressure, heart rate, and ocular perfusion pressure were also monitored. RESULTS. The patients with glaucoma demonstrated an increase in diastolic blood pressure (P = 0.023), heart rate (P = 0.010), and mean ocular perfusion pressure (P = 0.039) during immersion of the tested hand in 40°C water. During cold provocation, the patients demonstrated a significant decrease in finger (P = 0.0003) and ocular blood flow (the parameter velocity measured at the temporal neuroretinal rim area; P = 0.021). Normal subjects did not demonstrate any blood flow or finger temperature changes during immersion of the tested hand in 40°C water (P > 0.05); however, they exhibited increases in systolic blood pressure (P = 0.034) and pulse pressure (P = 0.0009) and a decrease in finger blood flow (P = 0.0001) during cold provocation. In normal subjects, the ocular blood flow was unchanged during high- and low-temperature challenge. CONCLUSIONS. Cold provocation elicits a different blood pressure, and ocular blood flow response in patients with primary open-angle glaucoma compared with control subjects. These findings suggest a systemic autonomic failure and ocular vascular dysregulation in POAG patients

Linking the Conservation of Culture and Nature: A Case Study of Sacred Forests in Zimbabwe

This paper examines the role of traditional religious beliefs and traditional leaders in conserving remnant patches of a unique type of dry forest in the Zambezi Valley of northern Zimbabwe. We examined aerial photographs spanning more than three decades, interviewed and surveyed local residents, and met with communities to learn about the environmental history of the forests and the factors that have affected land use in the area. Our results show that forest loss is dramatically less in forests that are now considered sacred, or were in the past connected to sacred forests. This supports our hypothesis that traditional spiritual values have influenced human behavior affecting the forests, and have played a role in protecting them until now. We also found that rates of forest loss have been much higher in an area where traditional leaders are relatively disempowered within the post-independence political system compared to an area where traditional leaders have more power. These findings lead us to conclude that a strategy that links the conservation of culture and nature is likely to be more effective in conserving forests than a strategy that ignores traditional beliefs, values, and institutions

Admissibility Compared: The Reception of Incriminating Expert Evidence (i.e., Forensic Science) in Four Adversarial Jurisdictions

There is an epistemic crisis in many areas of forensic science. This crisis emerged largely in response both to the mobilization of a range of academic commentators and critics and the rise and influence of DNA typing. It gained popular and authoritative support through the influence of the National Academy of Science (NAS) and a surprisingly critical report produced under its auspices by a committee of the National Research Council (NRC). Interestingly, as this article endeavors to explain, the courts themselves seem to have played a rather indirect, inconsistent and ultimately ineffective role in the supervision and evaluation of forensic science evidence. Indeed, in the aftermath of recent criticism of the forensic sciences, this essay considers the effect of the dominant admissibility standards that operate in four common law jurisdictions. The revealing result seems to be that although admissibility standards vary across these jurisdictions, actual admissibility practices are remarkably consistent. In this article we will question the extent to which courts (and legal personnel) are able to meaningfully invigilate the use of forensic science evidence in criminal proceedings and consider some of the ideological commitments and institutional pressures that might lead judges in all jurisdictions to prefer inclusive approaches to incriminating expert opinions. In the first part of the article, we compare rules, jurisprudence and practices, across four jurisdictions: the United States, England and Wales, Canada, and Australia. All profoundly shaped by the English common law, these jurisdictions (and their sub- jurisdictions) tend to use a mixture of common law (e.g. England and Wales and Canada), judge-made rules (e.g. the U.S. Federal Rules of Evidence) and statutory schemes (e.g. the Australian Evidence Act 1995 Cth and many states in the United States) to regulate the admission of evidence, including expert opinion. These jurisdictions tend to maintain criminal trial processes that remain reasonably similar and facilitate broad brush comparisons. Our findings suggest that admissibility standards, including the first generation of reliability-based standards, seem to make little, if any, difference to (traditional) admissibility decision-making and practice. Allowing for some variation, the same sorts of forensic science evidence are admitted across all jurisdictions, even where the techniques are not demonstrably reliable and the jurisdiction in question has explicit reliability standards and other rules regulating the admission of expert opinion evidence. Moreover, it is our contention that the legal accommodation of the techniques considered in this article exemplifies a more general response to admissibility and the regulation of forensic science and medicine evidence. In the second part of the article we will consider possible explanations and some of the implications of our findings

Establishing a Taphonomic Research Facility in the United Kingdom

In May 2009, the University of Central Lancashire, UK, launched TRACES (Taphonomic Research in Anthropology—Centre for Experimental Study). This facility uses animal models in taphonomic research. The use of animal models facilitates wider studies of factors influencing decomposition than the low replicate numbers common to human cadaver studies. The establishment of dedicated facilities to carry out taphonomic research is long and complex. Whether the facility uses human cadavers, as in the United States, or animal models, as in the UK, the issues that arise can be common to both. These include commitment of resources, local community concerns, and planning and legal issues. However, the use of animal models also raises additional ethical and legislative concerns. These include environmental impact, animal welfare, biosecurity, and disposal activities. This article discusses the processes undertaken during the establishment of a taphonomic facility using animal models in the UK and demonstrates the level of commitment, enthusiasm, and perseverance required

Aboveground biomass corresponds strongly with drone-derived canopy height but weakly with greenness (NDVI) in a shrub tundra landscape

This is the author accepted manuscript. The final version is available from IOP Publishing via the DOI in this recordData accessibility: The data that support the findings of this study are openly available at the following DOI: https://doi.org/10.5285/61C5097B-6717-4692-A8A4-D32CCA0E61A9)Arctic landscapes are changing rapidly in response to warming, but future predictions are hindered by difficulties in scaling ecological relationships from plots to biomes. Unmanned aerial systems (UAS, hereafter 'drones') are increasingly used to observe Arctic ecosystems over broader extents than can be measured using ground-based approaches and facilitate the interpretation of coarse-grained remotely-sensed datasets. However, more information is needed about how drone-acquired remote sensing observations correspond with ecosystem attributes such as aboveground biomass. Working across a willow shrub-dominated alluvial fan at a focal study site in the Canadian Arctic, we conducted peak season drone surveys with a RGB camera and multispectral multi camera array to derive photogrammetric reconstructions of canopy and normalised difference vegetation index (NDVI) maps along with in situ point intercept measurements and biomass harvests from 36, 0.25 m2 plots. We found high correspondence between canopy height measured using in situ point intercept compared to drone-photogrammetry (concordance correlation coefficient = 0.808), although the photogrammetry heights were positively biased by 0.14 m relative to point intercept heights. Canopy height was strongly and linearly related to aboveground biomass, with similar coefficients of determination for point framing (R2 = 0.92) and drone-based methods (R2 = 0.90). NDVI was positively related to aboveground biomass, phytomass and leaf biomass. However, NDVI only explained a small proportion of the variance in biomass (R2 between 0.14 and 0.23 for logged total biomass) and we found moss cover influenced the NDVI-phytomass relationship. Biomass is challenging to infer from drone-derived NDVI, particularly in ecosystems where bryophytes cover a large proportion of the land surface. Our findings suggest caution with broadly attributing change in fine-grained NDVI to biomass differences across biologically and topographically complex tundra landscapes. By comparing structural, spectral and on-the-ground ecological measurements, we can improve understanding of tundra vegetation change as inferred from remote sensing.Natural Environment Research Council (NERC)Dartmouth CollegeAarhus University Research FoundationEuropean Union Horizon 202

Burden of disease and circulating serotypes of rotavirus infection in sub-Saharan Africa: systematic review and meta-analysis.

Two new rotavirus vaccines have recently been licensed in many countries. However, their efficacy has only been shown against certain serotypes commonly circulating in Europe, North America, and Latin America, but thought to be globally important. To assess the potential impact of these vaccines in sub-Saharan Africa, where rotavirus mortality is high, knowledge of prevalent types is essential because an effective rotavirus vaccine is needed to protect against prevailing serotypes in the community. We did two systematic reviews and two meta-analyses of the most recent published data on the burden of rotavirus disease in children aged under 5 years and rotavirus serotypes circulating in countries in sub-Saharan Africa. Eligible studies were selected from PubMed/Medline, Cochrane Library, EmBase, LILACS, Academic Search Premier, Biological Abstracts, ISI Web of Science, and the African Index Medicus. Depending on the heterogeneity, DerSimonian-Laird random-effects or fixed-effects models were used for meta-analyses. Geographical variability in rotavirus burden within countries in sub-Saharan Africa is substantial, and most countries lack information on rotavirus epidemiology. We estimated that annual mortality for this region was 243.3 (95% CI 187.6-301.7) deaths per 100,000 under 5 years (ie, a total of 300,000 children die of rotavirus infection in this region each year). The most common G type detected was G1 (34.9%), followed by G2 (9.1%), and G3 (8.6%). The most common P types detected were P[8] (35.5%) and P[6] (27.5%). Accurate information should be collected from surveillance based on standardised methods in these countries to obtain comparable data on the burden of disease and the circulating strains to assess the potential impact of vaccine introduction