A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy of the reported scan time after the start of centrifugation, the accuracy of the temperature calibration, and the accuracy of the radial magnification. The range of sedimentation coefficients obtained for BSA monomer in different instruments and using different optical systems was from 3.655 S to 4.949 S, with a mean and standard deviation of (4.304 ± 0.188) S (4.4%). After the combined application of correction factors derived from the external calibration references for elapsed time, scan velocity, temperature, and radial magnification, the range of s-values was reduced 7-fold with a mean of 4.325 S and a 6-fold reduced standard deviation of ± 0.030 S (0.7%). In addition, the large data set provided an opportunity to determine the instrument-to-instrument variation of the absolute radial positions reported in the scan files, the precision of photometric or refractometric signal magnitudes, and the precision of the calculated apparent molar mass of BSA monomer and the fraction of BSA dimers. These results highlight the necessity and effectiveness of independent calibration of basic AUC data dimensions for reliable quantitative studies

Consumer preferences in the design of airport passenger areas

In recent decades, commercial developments have become increasingly important for the overall profit of airports. However, little is known about consumer preferences regarding the design of passenger areas, which is striking as the design of terminal buildings affects consumers' emotional state and shopping behaviour. The aim of this study was therefore to investigate how architectural design characteristics are valued by airport passengers, using visualizations of hypothetical passenger areas. Discrete choice experiments were used to investigate passenger preferences for eight design characteristics. Data on 346 passengers were collected in June 2008 in departure and transfer areas at Amsterdam Airport Schiphol. Analyses showed that passengers preferred a passenger area with a curvilinear roof, a curved layout, the presence of greenery, no decoration reflecting the distinctiveness of Holland, warm lighting, wide dimensions and white materials. Signage had no influence.Real Estate & HousingArchitecture and The Built Environmen

Meeting places: drivers of change in Australian Aboriginal cultural institutions

Since the 1970s, there has been a fraught yet hopeful Aboriginal cultural resurgence in Australia. An element of this movement has been the establishment of Aboriginal art centres and cultural centres across Australia. Using a comparative approach to Aboriginal art centres, this paper analyses the appearance and characteristics of the more recent Aboriginal cultural centres. The methods used are a review of the literature on Aboriginal art centres, and for the less-researched Aboriginal cultural centres, a case study. This paper posits that cultural centre characteristics are shaped through the formation of alliances made possible by the advent of land rights, an Aboriginal cultural turn amongst Aboriginal and non-Aboriginal people, and changing approaches to regional development. While not themselves a movement that will lead to socio-economic change, these types of arts and heritage projects are aligned to such movements. With a larger scale and more central locations, Aboriginal cultural centres open up opportunities for larger and more diverse alliances, and therefore new opportunities for Aboriginal people’s participation, activism and expression

Dealing with urban terror: heritages of control, varieties of intervention, strategies of research

The events of September 11th bring urgency to problems of urban security, both in terms of finding ways to protect cities from attacks by terrorists and also protecting urban life from repressive measures that form in reaction to those attacks. We outline a rationale for urbanists to participate in analysis and policy-formulation on security issues and examine the utility of past urban research strategies, including criminology, in terms of their relevance to the current challenge. We suggest principles to guide future urban policy in light of past experiences. Copyright Joint Editors and Blackwell Publishing Ltd 2003.