Acoustodynamometry : An innovating non-invasive technique used to measure tendon load during movement

A novel technique was developed to measure the tendon load in people and in horses in motion. This non-invasive technique is based on a relation, demonstrated experimentally in vitro on isolated tendons and limbs, between the velocity of ultrasound in the tendon and the load to which it is subjected. A prototype was created to examine the equine superficial digital flexor tendon. The reproducibility of these measurements assessed in five horses was very good in a given subject, but marked differences were observed between the animals, in terms of plot shape and ultrasound velocity. These differences appeared directly related to the horse’s digital standing conformation. The technique thus validated was then used to examine the effect of four types of orthopaedic shoes compared to a standard shoe, on hard as well as soft ground.Un procédé original de mesure de la force qui s'exerce dans un tendon, chez le Cheval ou l'Homme en mouvement, a été mis au point. Cette technique non-invasive repose sur une relation, démontrée expérimentalement in vitro, sur tendons et membres isolés, entre la vitesse des ultrasons dans un tendon et la force qui s'applique sur celui-ci. Un prototype adapté au tendon fléchisseur superficiel du doigt du cheval a été réalisé. La reproductibilité des mesures, évaluée chez cinq chevaux, au pas et au trot, est très bonne chez un même individu, mais il existe des différences nettes entre sujets, à la fois dans la forme des tracés et dans les niveaux de vitesse des ultrasons observés. Ces différences sont apparues étroitement liées à l'aplomb digital des chevaux. Le procédé ainsi validé a permis d'évaluer l'effet de quatre types de ferrures correctrices, comparées à un fer standard, sur sol dur et sur sol meuble

Comparing subjective and objective evaluation of show jumping competition and warm-up arena surfaces

The development of safety and quality standards for equestrian surfaces needs to be based on objective, repeatable measurements which allow comparisons between surfaces. These measurements should incorporate the assessment of surface performance by riders. This study provides data from objective and subjective assessment of functional properties of high-level show jumping competition and warm-up arenas. Twenty-five arenas in nine international show jumping events were evaluated by mechanical insitu testing with a surface tester, rider assessments using visual analogue scales (198 riders provided 749 arena evaluations), descriptions of arena constructions and by laboratory tests of surface material. Mixed models were used to present subjective evaluation of rider perception of the functional properties for each arena while controlling for rider and event. The association between objective and subjective assessments were also explored creating mixed models, controlling for rider and event. Mechanical measurements of impact firmness, and to a lesser extent cushioning and grip, had a significant positive association with the riders’ perception. Responsiveness as assessed by the Orono biomechanical surface tester (OBST) was negatively associated with the riders’ perceptions, which suggests riders and the OBST had different concepts of this functional property and that further developments of the OBST might be necessary. Objectively measured uniformity showed no useful association with riders’ perception. Even though arena assessments were made by top level riders, a substantial inter-rider variation was demonstrated

First application of an axial speed of sound measurement technique in the monitoring of tendon healing

PublishedJournal ArticleN/AInstitut National de la Recherche AgronomiqueRe´gion Basse NormandieDirection Ge´ne´rale de l’Enseignement et de la Recherch

Axial speed of sound for the monitoring of injured equine tendons: a preliminary study.

PublishedJournal ArticleResearch Support, Non-U.S. Gov'tEquine superficial digital flexor tendons (SDFT) are often injured, and they represent an excellent model for human sport tendinopathies. While lesions can be precisely diagnosed by clinical evaluation and ultrasonography, a prognosis is often difficult to establish; the knowledge of the injured tendon's mechanical properties would help in anticipating the outcome. The objectives of the present study were to compare the axial speed of sound (SOS) measured in vivo in normal and injured tendons and to investigate their relationship with the tendons' mechanical parameters, in order to assess the potential of quantitative axial ultrasound to monitor the healing of the injured tendons. SOS was measured in vivo in the right fore SDFTs of 12 horses during walk, before and 3.5 months after the surgical induction of a bilateral core lesion. The 12 horses were then euthanized, their SDFTs isolated and tested in tension to measure their elastic modulus and maximal load (and corresponding stress). SOS significantly decreased from 2179.4 ± 31.4 m/s in normal tendons to 2065.8 ± 67.1 m/s 3.5 months after the surgical induction, and the tendons' elastic modulus (0.90 ± 0.17 GPa) was found lower than what has been reported in normal tendons. While SOS was not correlated to tendon maximal load and corresponding stress, the SOS normalized on its value in normal tendons was correlated to the tendons' elastic modulus. These preliminary results confirm the potential of axial SOS in helping the functional assessment of injured tendon.Direction Générale de l’Enseignement et de la Recherche (French Ministry of Agriculture)Région Basse-NormandieInstitut National de la Recherche AgronomiqueAgence Nationale de la Recherch

Axial speed of sound is related to tendon's nonlinear elasticity.

PublishedJournal ArticleResearch Support, Non-U.S. Gov'tAxial speed of sound (SOS) measurements have been successfully applied to noninvasively evaluate tendon load, while preliminary studies showed that this technique also has a potential clinical interest in the follow up of tendon injuries. The ultrasound propagation theory predicts that the SOS is determined by the effective stiffness, mass density and Poisson's ratio of the propagating medium. Tendon stiffness characterizes the tissue's mechanical quality, but it is often measured in quasi-static condition and for entire tendon segments, so it might not be the same as the effective stiffness which determines the SOS. The objectives of the present study were to investigate the relationship between axial SOS and tendon's nonlinear elasticity, measured in standard laboratory conditions, and to evaluate if tendon's mass density and cross-sectional area (CSA) affect the SOS level. Axial SOS was measured during in vitro cycling of 9 equine superficial digital tendons. Each tendon's stiffness was characterized with a tangent modulus (the continuous derivative of the true stress/true strain curve) and an elastic modulus (the slope of this curve's linear region). Tendon's SOS was found to linearly vary with the square root of the tangent modulus during loading; tendon's SOS level was found correlated to the elastic modulus's square root and inversely correlated to the tendon's CSA, but it was not affected by tendon's mass density. These results confirm that tendon's tangent and elastic moduli, measured in laboratory conditions, are related to axial SOS and they represent one of its primary determinants.Direction Générale de l’Enseignement et de la Recherche (French Ministry of Agriculture)Région Basse-NormandieInstitut National de la Recherche Agronomiqu

True stress and Poisson's ratio of tendons during loading.

PublishedJournal ArticleResearch Support, Non-U.S. Gov'tExcessive axial tension is very likely involved in the aetiology of tendon lesions, and the most appropriate indicator of tendon stress state is the true stress, the ratio of instantaneous load to instantaneous cross-sectional area (CSA). Difficulties to measure tendon CSA during tension often led to approximate true stress by assuming that CSA is constant during loading (i.e. by the engineering stress) or that tendon is incompressible, implying a Poisson's ratio of 0.5, although these hypotheses have never been tested. The objective of this study was to measure tendon CSA variation during quasi-static tensile loading, in order to assess the true stress to which the tendon is subjected and its Poisson's ratio. Eight equine superficial digital flexor tendons (SDFT, about 30cm long) were tested in tension until failure while the CSA of each tendon was measured in its metacarpal part by means of a linear laser scanner. Axial elongation and load were synchronously recorded during the test. CSA was found to linearly decrease with strain, with a mean decrease at failure of -10.7±2.8% (mean±standard deviation). True stress at failure was 7.1-13.6% higher than engineering stress, while stress estimation under the hypothesis of incompressibility differed from true stress of -6.6 to 2.3%. Average Poisson's ratio was 0.55±0.12 and did not significantly vary with load. From these results on equine SDFT it was demonstrated that tendon in axial quasi-static tension can be considered, at first approximation, as an incompressible material.Direction Générale de l’Enseignement et de la Recherche (French Ministry of Agriculture)Région Basse-NormandieInstitut National de la Recherche Agronomiqu

COMPARISON OF TWO PREPARATION PROCEDURES (HARROWING VS. ROLLING) APPLIED TO AN EQUESTRIAN SPORT ARENA: EFFECTS ON THE DYNAMIC VARIABLES IN 3 HORSES LANDING AFTER A JUMP

The purpose of this study was to compare the dynamic variables recorded on 3 horses landing after a jump on an arena surface (sand & fibre mix) after application of two classical preparation procedures: harrowing and rolling. Each horse, equipped with a dynamometric horseshoe and an accelerometer on its right forehoof, performed 6 jumps alternatively on each prepared surface, landing alternatively at right and left lead. The maximal vertical deceleration and the energy of vibrations (>50Hz) at impact significantly decreased with harrowing, on both limbs. The braking force and braking loading rate were greater on the rolled surface but only in the leading limb. The vertical loading rate and, in the leading limb only, the maximal vertical force, were significantly larger on the rolled surface, which suggests that preparation affected the surface deeper than expected

The Sequisol project: biomechanical eval uation of the effects of equestrian track surfaces on the equine locomotor system

The quality of ground surfaces conditions equine locomotion and can be a risk factor for osteoarticular and tendinous lesions. A novel protocol of biomechanical measurements has been developed to characterize the effects of ground surfaces on the locomotor system and locomotion of harnessed trotter horses under training conditions. This protocol is based on the simultaneous use of a 3D dynamometric horseshoe, a 3D accelerometer, a superficial digital flexor (SDF) tendon force ultrasonic sensor, inertial measurement units, and a high-speed camera. Three French trotters were used to compare two tracks at the Grosbois training centre (crushed sand and all-weather waxed track) ; biomechanical measurements were performed at a standardized speed (9.7 m/s, i.e. 35 km/h). The all-weather waxed track appeared as the most damping (shock absorbing), i.e. lesser impact deceleration and braking force and lesser maximal loading rate of the SDF tendon. However, the horse’s locomotor comfort (determined by the stride length) did not seem to be greater. The preliminary results of the tests performed since October 2006 as part of the Sequisol project (10 tracks tested on 5 different sites) confirm superior shock-absorbing properties as well as a “slower” characteristic during the braking phase for the all-weather waxed tracks, and generally speaking, the strong influence of maintenance conditions of the surfaces on the biomechanical results.La qualité du sol conditionne la locomotion d'un cheval, et peut aussi être un facteur de risque de lésions ostéo-articulaires et tendineuses. Un protocole original de mesures biomécaniques a été mis au point afin de caractériser l'effet des sols sur l'appareil locomoteur et la locomotion, chez le trotteur attelé, dans les conditions de l'entraînement. Ce protocole repose sur l'utilisation simultanée d'un fer dynamométrique 3D, d'un accéléromètre 3D, d'un capteur ultrasonore de force dans le tendon fléchisseur superficiel du doigt (perforé), de centrales de mesure inertielle et d'une caméra haute fréquence. Trois chevaux trotteurs français ont été utilisés pour comparer deux pistes du centre d'entraînement de Grosbois (sable concassé et sable fibré-huilé); les mesures biomécaniques ont été effectuées à vitesse standardisée (9,7 m/s, soit 35 km/h). La piste en fibré-huilé présente des propriétés d'amortissement plus importantes que la piste en sable concassé: la décélération à l'impact, la force de freinage et la vitesse de mise en tension maximale du tendon perforé sont plus faibles. En revanche, le confort locomoteur du cheval, apprécié notamment par la longueur de la foulée, n'est pas supérieur. Le bilan préliminaire des tests réalisés depuis octobre 2006 dans le cadre du projet Sequisol (dix pistes testées sur cinq sites différents) confirme le caractère plus amortissant mais aussi plus « lent », lors du freinage, des pistes en fibré-huilé et, plus généralement, l'influence forte des conditions d'entretien des sols sur les résultats biomécaniques

Science in brief: Highlights from the biomechanics and physiotherapy abstracts at the International Conference on Equine Exercise Physiology.

Although human observations of equine locomotion are as old as our relationship with the horse, today's scientists still have much to learn about horse–human interactions. Two approaches are commonly used to study equine biomechanics and both were evident in abstracts presented at the International Conference on Equine Exercise Physiology (ICEEP) 2014. One approach is to use simplified methods of measurement and analysis that provide simple but meaningful objective information that can ultimately be used by the clinician or practitioner. Alternatively, more complex equipment and techniques may be used that directly measure or infer loading on the equine musculoskeletal system to provide detailed structural and functional information. Whichever methods are used, it is important that they are reliable and robust and that the errors and limitations of the measurement system are fully recognised when interpreting data. In his keynote speech, Professor René van Weeren proposed that the biomechanical techniques available to scientists today provide a gateway to a better understanding of the horse–rider interaction that must ultimately improve equine welfare while maintaining peak performance. The abstracts presented in this Editorial therefore cover key topics that are relevant to welfare and performance, lameness and asymmetry, locomotion and sports performance, a focus on the axial system, and the foot

Proteomic Analysis of Tendon Extracellular Matrix Reveals Disease Stage- specific Fragmentation and Differential Cleavage of COMP ( Cartilage Oligomeric Matrix Protein)

During inflammatory processes the extracellular matrix (ECM) is extensively remodeled, and many of the constituent components are released as proteolytically cleaved fragments. These degradative processes are better documented for inflammatory joint diseases than tendinopathy even though the pathogenesis has many similarities. The aims of this study were to investigate the proteomic composition of injured tendons during early and late disease stages to identify disease-specific cleavage patterns of the ECM protein cartilage oligomeric matrix protein (COMP). In addition to characterizing fragments released in naturally occurring disease, we hypothesized that stimulation of tendon explants with proinflammatory mediators in vitro would induce fragments of COMP analogous to natural disease. Therefore, normal tendon explants were stimulated with IL-1β and prostaglandin E2, and their effects on the release of COMP and its cleavage patterns were characterized. Analyses of injured tendons identified an altered proteomic composition of the ECM at all stages post injury, showing protein fragments that were specific to disease stage. IL-1β enhanced the proteolytic cleavage and release of COMP from tendon explants, whereas PGE2 had no catabolic effect. Of the cleavage fragments identified in early stage tendon disease, two fragments were generated by an IL-1-mediated mechanism. These fragments provide a platform for the development of neo-epitope assays specific to injury stage for tendon disease