55 research outputs found
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.Instituto Butantan Laboratório Especial de MicrobiologiaUniversidade Federal de São Paulo (UNIFESP) Imunologia e Parasitologia Departamento de MicrobiologiaRobert Koch Institut Division of Emerging Bacterial PathogensInstituto de Saúde ColetivaUniversidade Federal da Bahia Departamento de PediatriaUNIFESP, Imunologia e Parasitologia Depto. de MicrobiologiaSciEL
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
How patterns spread:The to-infinitival complement as a case of diffusional change, or 'to-infinitives, and beyond'
<i>The Role Played by Analogy in Processes of Language Change: The Case of English</i> Have-to <i>Compared to Spanish </i>Tener-que
HIV-1 seroprevalence in the general population of Salvador, Bahia State, Northeast Brazil
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Materials and electrode configuration strategies for flexible, printable, and implantable energy storage
Global energy consumption is projected to double from its 1990 level by 2030. Although rechargeable lithium-ion batteries have powered mobile computing and telecommunications for two decades, their energy density is inadequate to meet rising demands for grid storage, and falls short of DOE targets for electric vehicles and the needs of new electronic devices. As a result, the lithium-sulfur (Li-S) system has emerged as a leading “Beyond Lithium Ion” candidate. Simultaneously, the impetus for a comprehensive “Internet of Things” that interactively monitors and controls energy use, infrastructure, and human health has been consistently hampered by a lack of batteries that are small, thin, flexible, implantable, high-energy density, and cheaply manufactured to power the multitude of required devices. This dissertation addresses three aspects of these dilemmas. First, an elastic, conductive, and electroactive polymer nanocomposite comprising polypyrrole and polyurethane (PPyPU) is developed to serve as a binder for flexible Li-S cathodes. After fifty flex/bend cycles, the cathodes provide high capacity with essentially no capacity-fade for 100 cycles at high sulfur loadings. The electroactive PPy in the binder is believed to stabilize dissolved polysulfides, while the elastic PU accommodates the significant sulfur expansion which is known to compromise Li-S cathode integrity during charge/discharge. Second, a process is presented for producing extrusion-printed carbon nanotube-based electrodes for Li-S cathodes. Although printing provides a high-throughput, inexpensive, top-down, “green” alternative to industrial microfabrication, it has been infrequently applied to batteries, and the few reports of printed batteries were based on low energy-density materials. Therefore, multiwall carbon nanotube inks are formulated for printing microelectrodes to be utilized as electrodeposition scaffolds for high-loading Li/polysulfide catholytes. The resulting lithium-sulfur cathodes are shown to meet industrial benchmarks for portable and wearable electronics. In concert, sulfur-infused single-wall carbon nanotubes (S@SWNT) are used for inkjet-printed thin-film cathodes as proof-of-concept for integrated, printing-based nanomanufacturing. Third, an electroactive bio-nanocomposite comprising purely endogenous materials (dopamine and hyaluronic acid) is synthesized and characterized in vitro as a potentially implantable energy-storage material. The dopamine-hyaluronic acid (DAHA) hydrogel composite can be electropolymerized to create a pseudocapacitive biopolymer, p(DAHA), that exhibits catechol−quinone interconversion, high pseudocapacitance and discharge capacity, and stable, long-term electroactivity for 400 cycles. These characteristics predispose it for bioelectronic energy storage, i.e., as a supercapacitor or, when coupled with an implantable Ag/AgCl electrode, a biobattery with an operating voltage of ∼0.85 V.Chemical Engineerin
Bioelectronic Energy Storage: A Pseudocapacitive Hydrogel Composed of Endogenous Biomolecules
Advances
in bioelectronics have produced implantable devices for
in vivo biosensing and therapeutics, but batteries for implantable
devices currently require bulky metal cases to sequester toxic electrolytes
and immunogenic active materials; therefore, development of new materials
is paramount for safety and miniaturization. Implantable batteries
could be fully biocompatible if they exclusively comprised endogenous
materials. Accordingly, we present an energy-storage material fabricated
entirely from endogenous biomolecules via one-step carbodiimide conjugation
of dopamine (DA) to hyaluronic acid (HA). The DAHA composite can be
electropolymerized to create a pseudocapacitive biopolymer, p(DAHA),
that exhibits catechol–quinone interconversion, stability,
long-term electroactivity for 400 cycles, and high pseudocapacitance
(up to ∼900 F g<sup>–1</sup>) and discharge capacity
(∼130 mAh g<sup>–1</sup> at ∼10 A g<sup>–1</sup>). These characteristics predispose it for bioelectronic
energy storage, i.e., as a supercapacitor or, when coupled with an
implantable Ag/AgCl electrode, a biobattery with an operating voltage
of ∼0.85 V
And the Injun goes “How!”: Representations of American Indian English in white public space
Nerve Growth Factor-Immobilized Electrically Conducting Fibrous Scaffolds for Potential Use in Neural Engineering Applications
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