478 research outputs found

    Temporal Expression Pattern Of The Insulin-like Growth Factor Ii And Fibroblast Growth Factor Transcripts In Vian Embryogenesis

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    In this study, the abundance of IGF-II and bFGF transcripts was estimated in the chicken embryos using the competitive RT-PCR analysis. Significant enhancements in the abundance of IGF-II mRNA were observed at stages HH1 and 5, and a new accumulation in these levels was observed at stage HH18 in comparison to the basal levels. The abundance of bFGF mRNA increased significantly at stages HH18 and 20, followed by an upregulation in the expression of these transcripts at stage HH26. These findings provided important information about the temporal expression pattern of IGF-II and bFGF transcripts in the whole chicken embryos during in ovo development.515949955Bass, J., Oldham, J., Sharma, M., Kambadur, R., Growth factors controlling muscle development (1999) Dom. An. Endocrinol, 17, pp. 191-197Borja, A.J.M., Zeller, R., Meyers, C., Expression of alternatively spliced bFGF coding exons and antisense mRNAs during chicken embryogenesis (1993) Dev. Biol, 157, pp. 110-118Castelli, R., Porro, F., Tarsia, P., The heparins and cancer: Review of clinical trials and biological properties (2004) Vas. Med, 9, pp. 205-213Chomczynski, P., Sacchi, N., Single step method of RNA isolation by acid guanidinium thiocyanatephenol chloroform extraction (1987) Analytical Biochem, 162, pp. 156-159Cohn, M.J., Izpisúa-Belmonte, J.C., Abud, H., Heath, J.K., Tickle, C., Fibroblast growth factors induce additional limb development from the flank of chick embryos (1995) Cell, 80, pp. 739-746Cook, R.D., Weisberg, S., Transforming a response variable for linearity (1994) Biometrika, 81, pp. 731-737Darling, D.C., Brickell, P.M., Nucleotide sequence and genomic structure of the chicken insulin-like growth factor-II (IGF-II) coding region (1996) Gen. Comp. Endocrinol, 102, pp. 283-287Denley, A., Cosgrove, L., Booker, G., Wallace, J., Forbes, B., Molecular interactions of the IGF system (2005) Cytokine Growth Factor Rev, 16, pp. 421-439Florini, J.R., Magri, K.A., Ewton, D.Z., James, P.L., Grindstaff, K., Rotwein, P., Spontaneous differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II (1991) J. Biol. Chem, 266, pp. 15917-15923Florini, J.R., Ewton, D.Z., Cooligan, S.A., Growth hormone and the insulin-like growth factor system in myogenesis (1996) Endocrine Rev, 1795, pp. 481-517Gabriel, J.E., Javiel, H.A., Alvares, L.A., Schmidt, G., Coutinho, L.L., In situ detection of the myogenic factor MyoD in whole chicken embryos (2000) Genet. Mol. Biol, 23, pp. 145-148Gabriel, J.E., Alvares, L.E., Gobet, M.C., de Paz, C.C.P., Packer, I.U., Macari, M., Coutinho, L.L., Expression of MyoD, myogenin, myostatin and Hsp70 transcripts in chicken embryos submitted to mild cold or heat (2003) J. Thermal Biol, 28, pp. 261-269Hamburger, V., Hamilton, H.L., A series of normal stages in the development of the chick embryo (1951) J. Morphol, 88, pp. 49-92Hannon, K., Smith, C.K., Bales, K.R., Santerre, R.F., Temporal and quantitative analysis of myogenic regulatory and growth factor gene expression in the developing mouse embryo (1992) Dev. Biol, 151, pp. 137-144Kocamis, H., Killefer, J., Expression profiles of IGF-I, IGF-II, bFGF and TGF-b2 growth factors during chicken embryonic development (2003) Turk J Vet Anim Sci, 27, pp. 367-372Kost, T.A., Theodorakis, N., Hughes, S.H., The nucleotide sequence of the chick cytoplasmic betaactin gene (1983) Nucleic Acids Res, 11, pp. 8287-8301Muramatsu, M., Yamada, M., Takai, S., Miyazaki, M., Suppression of basic fibroblast growth factor-induced angiogenesis by a specific chymase inhibitor, BCEAB, through the chymase-angiotensin-dependent pathway in hamster sponge granulomas (2002) Br. J. Pharmacol, 137, pp. 554-560Ohuchi, H., Nakagawa, T., Yamamoto, A., Araga, A., Ohata, T., Isbimam, Y., Yoshioka, H., Noji, S., The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor (1997) Development, 113, pp. 1419-1434Pirskanen, A., Kiefer, J.C., Hauschka, S.D., IGFs, insulin, Shh, bFGF, and TGF-beta 1 interact synergistically to promote somite myogenesis in vitro (2000) Dev. Biol, 224, pp. 189-203Pownall, M.E., Emerson Jr., C.J., Sequential activation of three myogenic regulatory genes during somite mophogenesis in quail embryos (1992) Dev. Biol, 151, pp. 67-79Sambrook, J., Fritsch, E.F., Maniatis, T. Extraction, purification, analysis of messenger RNA from eukaryotic cells. In: Ford, N. Molecular cloning: a laboratory manual. 2.ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989, pp.7.40-7.87Sanchez, C.L., Rodriguez-Gallardo, L., Alvarez, I.S., Climent, V., Garcia-Martinez, V., Effects of growth factors on the commitment of chick blastoderm (2001) Int. J. Dev. Biol, 45, pp. S109-S110SAS Institute. SAS/STAT User's guide. Online Version. 8.ed. Cary: SAS Institute, 1999Szebenyi, G., Fallon, W., Fibroblast growth factors as multifunctional signaling factors (1999) Int Rev Citol, 185, pp. 45-106Tsai, S., Wiltbank, M.C., Quantification of mRNA using competitive RT-PCR with standard curve methodology (1996) Biotechniques, 21, pp. 862-86

    A comparative analysis of thermophysical properties correlations for n-paraffins to be used in wax precipitation modeling

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    The performance of a thermodynamic wax precipitation model strongly depends upon the n-paraffin thermophysical properties used. In order to estimate them, several correlations have been proposed, and their values have a great impact on both calculated wax disappearance temperature (WDT) and amount of wax precipitated at each temperature (WPC). The main goal of this work is to evaluate the correlations available for the relevant thermophysical properties aiming at achieving a reliable wax precipitation modeling. The methodology used involves the direct comparison of the correlations with the values of pure n-paraffin properties, and indirect evaluation by their use in the estimation of wax disappearance temperatures, the amount of wax precipitated at each temperature, and DSC experimental curves. This study contemplates two thermodynamic approaches for paraffin precipitation: the solid solution (SS), which considers the formation of one solid solution; and the multisolid phase model (MS), that assumes that each solid phase consists of a pure component.publishe

    The one-dimensional XXZ model with long-range interactions

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    The one-dimensional XXZ model (s=1/2, N sites) with uniform long-range interactions among the transverse components of the spins is considered. The Hamiltonian of the model is explicitly given by H=Jj=1N(sjxsj+1x+sjysj+1y)(I/N)j,k=1Nsjzskzhj=1Nsjz,H=J\sum_{j=1}^{N}(s_{j}^{x}s_{j+1}^{x}+s_{j}^{y}s_{j+1}^{y}) -(I/N)\sum_{j,k=1}^{N}s_{j}^{z}s_{k}^{z}-h\sum_{j=1}^{N}s_{j}^{z}, where the sx,y,zs^{x,y,z} are half the Pauli spin matrices. The model is exactly solved by applying the Jordan-Wigner fermionization, followed by a Gaussian transformation. In the absence of the long-range interactions (I=0), the model, which reduces to the isotropic XY model, is known to exhibit a second-order quantum phase transition driven by the field at zero temperature. It is shown that in the presence of the long-range interactions (I different from 0) the nature of the transition is strongly affected. For I>0, which favours the ordering of the transverse components of the spins, the transition is changed from second- to first-order, due to the competition between transverse and xy couplings. On the other hand, for I<0, which induces complete frustration of the spins, a second-order transition is still present, although the system is driven out of its usual universality class, and its critical exponents assume typical mean-field values.Comment: 5 pages, 1 figure, presented at ICM2000, to be published in the Proceedings (Journal of Magnetism & Magnetic Materials

    Photoinduced antibacterial activity of the essential oils from Eugenia brasiliensis lam and Piper mosenii C. DC. by blue led light

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    The objective of this work was to evaluate the phytochemical composition and the antibacterial and antibiotic-modulating activities of the essential oils of Eugenia brasiliensis Lam (OEEb) and Piper mosenii C. DC (OEPm) singly or in association with blue LED (Light-emitting diode) light. The antibacterial and antibiotic-modulatory activities of the essential oils on the activity of aminoglycosides were evaluated to determine the minimum inhibitory concentration (MIC, \u3bcg/mL) in the presence or absence of exposure to blue LED light. The chemical analysis showed \u3b1-pinene and bicyclogermacrene as major constituents of OEPm, whereas \u3b1-muurolol was the main compound of OEEb. Both OEEb and OEPm showed MIC 65 512 \u3bcg/mL against the strains under study. However, the association of these oils with the blue LED light enhanced the action of the aminoglycosides amikacin and gentamicin. In conclusion, the association of aminoglycosides with the blue LED light and essential oils was effective against resistant bacteria

    Measurements of long-range near-side angular correlations in sNN=5\sqrt{s_{\text{NN}}}=5TeV proton-lead collisions in the forward region

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    Two-particle angular correlations are studied in proton-lead collisions at a nucleon-nucleon centre-of-mass energy of sNN=5\sqrt{s_{\text{NN}}}=5TeV, collected with the LHCb detector at the LHC. The analysis is based on data recorded in two beam configurations, in which either the direction of the proton or that of the lead ion is analysed. The correlations are measured in the laboratory system as a function of relative pseudorapidity, Δη\Delta\eta, and relative azimuthal angle, Δϕ\Delta\phi, for events in different classes of event activity and for different bins of particle transverse momentum. In high-activity events a long-range correlation on the near side, Δϕ0\Delta\phi \approx 0, is observed in the pseudorapidity range 2.0<η<4.92.0<\eta<4.9. This measurement of long-range correlations on the near side in proton-lead collisions extends previous observations into the forward region up to η=4.9\eta=4.9. The correlation increases with growing event activity and is found to be more pronounced in the direction of the lead beam. However, the correlation in the direction of the lead and proton beams are found to be compatible when comparing events with similar absolute activity in the direction analysed.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-040.htm

    Study of the production of Λb0\Lambda_b^0 and B0\overline{B}^0 hadrons in pppp collisions and first measurement of the Λb0J/ψpK\Lambda_b^0\rightarrow J/\psi pK^- branching fraction

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    The product of the Λb0\Lambda_b^0 (B0\overline{B}^0) differential production cross-section and the branching fraction of the decay Λb0J/ψpK\Lambda_b^0\rightarrow J/\psi pK^- (B0J/ψK(892)0\overline{B}^0\rightarrow J/\psi\overline{K}^*(892)^0) is measured as a function of the beauty hadron transverse momentum, pTp_{\rm T}, and rapidity, yy. The kinematic region of the measurements is pT<20 GeV/cp_{\rm T}<20~{\rm GeV}/c and 2.0<y<4.52.0<y<4.5. The measurements use a data sample corresponding to an integrated luminosity of 3 fb13~{\rm fb}^{-1} collected by the LHCb detector in pppp collisions at centre-of-mass energies s=7 TeV\sqrt{s}=7~{\rm TeV} in 2011 and s=8 TeV\sqrt{s}=8~{\rm TeV} in 2012. Based on previous LHCb results of the fragmentation fraction ratio, fΛB0/fdf_{\Lambda_B^0}/f_d, the branching fraction of the decay Λb0J/ψpK\Lambda_b^0\rightarrow J/\psi pK^- is measured to be \begin{equation*} \mathcal{B}(\Lambda_b^0\rightarrow J/\psi pK^-)= (3.17\pm0.04\pm0.07\pm0.34^{+0.45}_{-0.28})\times10^{-4}, \end{equation*} where the first uncertainty is statistical, the second is systematic, the third is due to the uncertainty on the branching fraction of the decay B0J/ψK(892)0\overline{B}^0\rightarrow J/\psi\overline{K}^*(892)^0, and the fourth is due to the knowledge of fΛb0/fdf_{\Lambda_b^0}/f_d. The sum of the asymmetries in the production and decay between Λb0\Lambda_b^0 and Λb0\overline{\Lambda}_b^0 is also measured as a function of pTp_{\rm T} and yy. The previously published branching fraction of Λb0J/ψpπ\Lambda_b^0\rightarrow J/\psi p\pi^-, relative to that of Λb0J/ψpK\Lambda_b^0\rightarrow J/\psi pK^-, is updated. The branching fractions of Λb0Pc+(J/ψp)K\Lambda_b^0\rightarrow P_c^+(\rightarrow J/\psi p)K^- are determined.Comment: 29 pages, 19figures. All figures and tables, along with any supplementary material and additional information, are available at https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-032.htm

    BB flavour tagging using charm decays at the LHCb experiment

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    An algorithm is described for tagging the flavour content at production of neutral BB mesons in the LHCb experiment. The algorithm exploits the correlation of the flavour of a BB meson with the charge of a reconstructed secondary charm hadron from the decay of the other bb hadron produced in the proton-proton collision. Charm hadron candidates are identified in a number of fully or partially reconstructed Cabibbo-favoured decay modes. The algorithm is calibrated on the self-tagged decay modes B+J/ψK+B^+ \to J/\psi \, K^+ and B0J/ψK0B^0 \to J/\psi \, K^{*0} using 3.0fb13.0\mathrm{\,fb}^{-1} of data collected by the LHCb experiment at pppp centre-of-mass energies of 7TeV7\mathrm{\,TeV} and 8TeV8\mathrm{\,TeV}. Its tagging power on these samples of BJ/ψXB \to J/\psi \, X decays is (0.30±0.01±0.01)%(0.30 \pm 0.01 \pm 0.01) \%.Comment: All figures and tables, along with any supplementary material and additional information, are available at http://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-027.htm
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