Morphological characterization of two species of Abelmoschus: Abelmoschus esculentus and Abelmoschus caillei

Abelmoschus esculentus (L.) Moench. (okra) and Abelmoschus caillei (A.Chev.) Stevels (West African okra) are commonly grown as vegetable crops in southern Nigeria. This study examined the intra- and interspecific relationships between the two species, using seven accessions of A. esculentus and eight of A. caillei. Twelve quantitative and 12 qualitative characters were pooled for the analyses. Leaf characters were measured at the inception of the third leaf, floral characters at inception of flowering. Growth pattern was indeterminate for A. esculentus and determinate for A. caillei; stem was weak and procumbent for A. esculentus, stiff and erect for A. caillei; internode length was short/moderate in A. esculentus, long in A. caillei. Epicalyses terminated at the onset of the fruit in A. esculentus, but were hard and persistent in A. caillei; fruit shape was fusiform in A. esculentus, ovoid/oblong in A. caillei, erect in A. esculentus, pendulous in A. caillei. Flowering period was longer in A. caillei (>43 days) than in A. esculentus (maximum of 43 days). A. caillei produced more fruits (77%) than A. esculentus (44%); taller plants were recorded for A. caillei (34.50–52.20 cm) than for A. esculentus (21.30–30.10 cm). Greater intraspecific variation was observed in A. esculentus (five clusters) than in A. caillei (three clusters). The species showed closer interspecific relationship at higher Euclidean distance

Isotopic Studies of Contaminant Transport at the Hanford Site,WA

Processes of fluid flow and chemical transport through thevadose zone can be characterized through the isotopic systematics ofnatural soils, minerals, pore fluids and groundwater. In thiscontribution, we first review our research using measured isotopicvariations, due both to natural and site related processes, of theelements H, O, N, Sr and U, to study the interconnection between vadosezone and groundwater contamination at the Hanford Site in south-centralWashington. We follow this brief review with a presentation of new datapertaining to vadose zone and groundwater contamination in the WMAT-TX-TY vicinity. Uranium (U) isotopic data for the C3832 core (WMA TX)indicates the involvement of processed natural U fuel, and links theobserved U contamination to the nearby single shelled tank TX-104. Thedata also precludes contamination from an early 1970 s TX-107 leak. Inthe case of the C4104 core (WMA T), the U isotopic data indicates amixture of processed natural and enriched U fuels consistent with themajor leak from T-106 in 1973. Uranium and Strontium isotopic data forthe cores also provides direct evidence for chemical interaction betweenhigh-pH waste fluid and sediment. Isotopic data for groundwater nitratecontamination in the vicinity of WMA-T strongly suggests high-level tankwaste (most likely from T-106) as the source of very high 99Tcconcentrations recently observed at the NE corner of WMAT

Telomere-led meiotic chromosome movements: recent update in structure and function

In S. cerevisiae prophase meiotic chromosomes move by forces generated in the cytoplasm and transduced to the telomere via a protein complex located in the nuclear membrane. We know that chromosome movements require actin cytoskeleton [13,31] and the proteins Ndj1, Mps3, and Csm4. Until recently, the identity of the protein connecting Ndj1-Mps3 with the cytoskeleton components was missing. It was also not known the identity of a cytoplasmic motor responsible for interacting with the actin cytoskeleton and a protein at the outer nuclear envelope. Our recent work [36] identified Mps2 as the protein connecting Ndj1-Mps3 with cytoskeleton components; Myo2 as the cytoplasmic motor that interacts with Mps2; and Cms4 as a regulator of Mps2 and Myo2 interaction and activities (Figure 1). Below we present a model for how Mps2, Csm4, and Myo2 promote chromosome movements by providing the primary connections joining telomeres to the actin cytoskeleton through the LINC complex.Fil: Lee, C. Y.. Oklahoma Medical Research Foundation; Estados UnidosFil: Bisig, Carlos Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Conrad, M. N.. Oklahoma Medical Research Foundation; Estados UnidosFil: Ditamo, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Almeida, L. Previato de. Oklahoma Medical Research Foundation; Estados UnidosFil: Dresser, M.E.. Oklahoma Medical Research Foundation; Estados UnidosFil: Pezza, Roberto. Oklahoma Medical Research Foundation; Estados Unido

Human spermatogenic failure purges deleterious mutation load from the autosomes and both sex chromosomes, including the gene DMRT1

Gonadal failure, along with early pregnancy loss and perinatal death, may be an important filter that limits the propagation of harmful mutations in the human population. We hypothesized that men with spermatogenic impairment, a disease with unknown genetic architecture and a common cause of male infertility, are enriched for rare deleterious mutations compared to men with normal spermatogenesis. After assaying genomewide SNPs and CNVs in 323 Caucasian men with idiopathic spermatogenic impairment and more than 1,100 controls, we estimate that each rare autosomal deletion detected in our study multiplicatively changes a man’s risk of disease by 10% (OR 1.10 [1.04–1.16], p,261023), rare X-linked CNVs by 29%, (OR 1.29 [1.11–1.50], p,161023), and rare Y-linked duplications by 88% (OR 1.88 [1.13–3.13], p,0.03). By contrasting the properties of our case-specific CNVs with those of CNV callsets from cases of autism, schizophrenia, bipolar disorder, and intellectual disability, we propose that the CNV burden in spermatogenic impairment is distinct from the burden of large, dominant mutations described for neurodevelopmental disorders. We identified two patients with deletions of DMRT1, a gene on chromosome 9p24.3 orthologous to the putative sex determination locus of the avian ZW chromosome system. In an independent sample of Han Chinese men, we identified 3 more DMRT1 deletions in 979 cases of idiopathic azoospermia and none in 1,734 controls, and found none in an additional 4,519 controls from public databases. The combined results indicate that DMRT1 loss-of-function mutations are a risk factor and potential genetic cause of human spermatogenic failure (frequency of 0.38% in 1306 cases and 0% in 7,754 controls, p = 6.261025). Our study identifies other recurrent CNVs as potential causes of idiopathic azoospermia and generates hypotheses for directing future studies on the genetic basis of male infertility and IVF outcomes.This work was partially funded by the Portuguese Foundation for Science and Technology FCT/MCTES (PIDDAC) and co-financed by European funds (FEDER) through the COMPETE program, research grant PTDC/SAU-GMG/101229/2008. IPATIMUP is an Associate Laboratory of the Portuguese Ministry of Science, Technology, and Higher Education and is partially supported by FCT. AML is the recipient of a postdoctoral fellowship from FCT (SFRH/BPD/73366/2010). CO is supported by a grant from the United States National Institutes of Health (R01 HD21244), JDS is supported by Damon Runyon Clinical Investigator Award, Alex's Lemonade Stand Foundation Epidemiology Award, and the Eunice Kennedy Shriver Children's Health Research Career Development Award NICHD 5K12HD001410. Support for humans studies and specimens were provided by the NIH/NIDDK George M. O'Brien Center for Kidney Disease Kidney Translational Research Core (P30DK079333) grant to Washington University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Search for lepton-flavor-violating τ→ℓV0\tau\to\ell V^0 decays at Belle

We have searched for neutrinoless $\tau$ lepton decays into $\ell$ and $V^0$, where $\ell$ stands for an electron or muon, and $V^0$ for a vector meson ($\phi$, $\omega$, $K^{*0}$, $\bar{K}^{*0}$ or $\rho^0$), using 543 fb$^{-1}$ of data collected with the Belle detector at the KEKB asymmetric-energy $e^+e^-$ collider. No excess of signal events over the expected background has been observed, and we set upper limits on the branching fractions in the range $(5.9-18) \times 10^{-8}$ at the 90% confidence level. These upper limits include the first results for the $\ell \omega$ mode as well as new limits that are significantly more restrictive than our previous results for the $\ell \phi$, $\ell K^{*0}$, $\ell \bar{K}^{*0}$ and $\ell \rho^0$ modes.Comment: 7 pages, 16 figure

Constraints on Dark Matter Annihilation in Clusters of Galaxies with the Fermi Large Area Telescope

Nearby clusters and groups of galaxies are potentially bright sources of high-energy gamma-ray emission resulting from the pair-annihilation of dark matter particles. However, no significant gamma-ray emission has been detected so far from clusters in the first 11 months of observations with the Fermi Large Area Telescope. We interpret this non-detection in terms of constraints on dark matter particle properties. In particular for leptonic annihilation final states and particle masses greater than ~200 GeV, gamma-ray emission from inverse Compton scattering of CMB photons is expected to dominate the dark matter annihilation signal from clusters, and our gamma-ray limits exclude large regions of the parameter space that would give a good fit to the recent anomalous Pamela and Fermi-LAT electron-positron measurements. We also present constraints on the annihilation of more standard dark matter candidates, such as the lightest neutralino of supersymmetric models. The constraints are particularly strong when including the fact that clusters are known to contain substructure at least on galaxy scales, increasing the expected gamma-ray flux by a factor of ~5 over a smooth-halo assumption. We also explore the effect of uncertainties in cluster dark matter density profiles, finding a systematic uncertainty in the constraints of roughly a factor of two, but similar overall conclusions. In this work, we focus on deriving limits on dark matter models; a more general consideration of the Fermi-LAT data on clusters and clusters as gamma-ray sources is forthcoming.Comment: accepted to JCAP, Corresponding authors: T.E. Jeltema and S. Profumo, minor revisions to be consistent with accepted versio

Observation of the Decays B0->K+pi-pi0 and B0->rho-K+

We report the observation of B^0 decays to the K^+pi^-pi^0 final state using a data sample of 78 fb^-1 collected by the Belle detector at the KEKB e^+e^- collider. With no assumptions about intermediate states in the decay, the branching fraction is measured to be (36.6^{+4.2}_{-4.3}+- 3.0)*10^-6.We also search for B decays to intermediate two-body states with the same K^+pi^-pi^0 final state. Significant B signals are observed in the rho(770)^- K^+ and K^*(892)^+pi^- channels, with branching fractions of (15.1^{+3.4+1.4+2.0}_{-3.3-1.5-2.1})* 10^-6 and (14.8^{+4.6+1.5+2.4}_{-4.4-1.0-0.9})* 10^-6, respectively. The first error is statistical, the second is systematic and the third is due to the largest possible interference. Contributions from other possible two-body states will be discussed. No CP asymmetry is found in the inclusive K^+pi^-pi^0 or rho^-K^+ modes, and we set 90% confidence level bounds on the asymmetry of -0.12<A_{CP}<0.26 and -0.18<A_{CP}<0.64, respectively.Comment: 18 pages, 7 figure

Low-Cycle Fatigue of Ultra-Fine-Grained Cryomilled 5083 Aluminum Alloy

The cyclic deformation behavior of cryomilled (CM) AA5083 alloys was compared to that of conventional AA5083-H131. The materials studied were a 100 pct CM alloy with a Gaussian grain size average of 315 nm and an alloy created by mixing 85 pct CM powder with 15 pct unmilled powder before consolidation to fabricate a plate with a bimodal grain size distribution with peak averages at 240 nm and 1.8 μm. Although the ultra-fine-grain (UFG) alloys exhibited considerably higher tensile strengths than those of the conventional material, the results from plastic-strain-controlled low-cycle fatigue tests demonstrate that all three materials exhibit identical fatigue lives across a range of plastic strain amplitudes. The CM materials exhibited softening during the first cycle, similar to other alloys produced by conventional powder metallurgy, followed by continual hardening to saturation before failure. The results reported in this study show that fatigue deformation in the CM material is accompanied by slight grain growth, pinning of dislocations at the grain boundaries, and grain rotation to produce macroscopic slip bands that localize strain, creating a single dominant fatigue crack. In contrast, the conventional alloy exhibits a cell structure and more diffuse fatigue damage accumulation

On velocity-dependent dark matter annihilations in dwarf satellites

Milky Way dwarf spheroidal satellites are a prime target for Dark Matter (DM) indirect searches. Recently the importance of possible long-range interactions has been recognized, as they can boost the expected DM gamma ray signal by orders of magnitude through an effect commonly known as the Sommerfeld enhancement. However, for such analyses precise modelling of DM phase-space distribution becomes crucial and can introduce large uncertainties in the final result. We provide a pioneering attempt towards a comprehensive investigation of these systematics. First, the DM halo profiles are constrained using Bayesian inference on the available stellar kinematic datasets with a careful treatment of observational and theoretical uncertainties. We consider both cuspy and cored parametric DM density profiles, together with the case of a non-parametric halo modelling directly connected to observable quantities along the line-of-sight. After reconsidering the study case of ergodic systems, the basic ingredient of all previous analyses, we investigate for the first time scenarios where DM particles are allowed to have anisotropic velocity distributions. Referring to a generalized J-factor, sensitive to velocity-dependent effects, an enhancement (suppression) with respect to the isotropic phase-space distributions is obtained for the case of tangentially (radially) biased DM particle orbits. We provide new estimates for J-factors for the eight brightest Milky Way dwarfs also in the limit of velocity-independent DM annihilation, in good agreement with previous results in literature, and derive data-driven lower-bounds based on the non-parametric modelling of the halo density. This work presents a state-of-the-art analysis of the aforementioned effects and falls within the interest of current and future experimental collaborations involved in DM indirect detection programs