Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal

Barbel (Barbus bocagei) a common species in Portuguese rivers was studied to assess the impact of water contamination on hepatic oxidative stress response, lipid peroxidation and histology. The Vizela River is a tributary of the Ave River, located in one of the most industrialized areas of Portugal. The oxidative stress biomarkers analyzed included superoxide dismutase, catalase, glutathione S-transferase, glutathione reductase, glucose 6‑phosphate dehydrogenase and xanthine oxidase activities. Levels of reduced glutathione and lipid peroxidation were also evaluated. Except xanthine oxidase activity, that did not show any alteration, all the other enzymatic activities were increased in the liver of barbel captured in the Vizela River when compared with reference barbel. While, no differences were observed for glutathione reductase content, lipid peroxidation was higher in barbel from the Vizela River. Liver histological alterations were determined and their severity scored. Though lymphocyte foci were only observed in Vizela River barbel, macrophage aggregates were also present in reference barbel, although the severity score was higher in Vizela fish. The results of this study show that barbel liver oxidative stress responses, lipid peroxidation and histology are sensitive to the contaminants present in Vizela River water and are valuable biomarkers for monitoring purposes.Barbos (Barbus bocagei), una especie común en los ríos portugueses, se utilizó para evaluar el impacto de la contaminación del agua en la respuesta hepática al estrés oxidativo, en la peroxidación lipídica y en la histología del órgano. El río Vizela es un afluente del río Ave, situado en una de las regiones más industrializadas de Portugal. Los biomarcadores de estrés oxidativo analizados fueron la actividad de las enzimas superóxido dismutasa, catalasa, glutatión S-transferasa, glutation reductasa, glucosa 6 fosfato deshidrogenasa y de la xantina oxidasa. Los niveles de glutatión reducido y de la peroxidación lipídica también fueron evaluados. Excepto la xantina oxidasa, que no mostró ninguna alteración, todas las otras actividades enzimáticas han sufrido incrementos en el hígado de los barbos capturados en el río Vizela, cuando se comparan con los barbos de referencia. No se observaron diferencias para el contenido de glutatión reductasa, pero la peroxidación lipídica fue mayor en los barbos del río Vizela. Las alteraciones en la histología hepática fueron identificadas y clasificadas de acuerdo con su gravedad. Mientras que los linfocitos de focos se observaron sólo en barbos del Río Vizela, los agregados de macrófagos también estuvieron presentes en barbos locales de referencia, aunque la gravedad de las alteraciones fue mayor en los peces del río Vizela. Los resultados de este estudio muestran que las respuestas de estrés oxidativo, la peroxidación lipídica y la histología hepática son sensibles a los contaminantes presentes en el agua del Río Vizela, demonstrando ser biomarcadores valiosos para propósitos de monitoreo

Evaluation of soil mechanic resistance and seed depth placement in a maize crop seed under different soil management practices

Com o objetivo de estudar a influência da resistência mecânica do solo na distribuição vertical de semente obtida por semeadores numa cultura de milho, realizou-se na campanha de 2011 um estudo de campo na região do Alentejo, sendo a operação de sementeira feita sob diferentes praticas culturais: mobilização convencional, mobilização mínima e sementeira direta. Em sementeira direta foram ainda testadas duas velocidades de trabalho, 4 km-1 e 6 km-1. A profundidade de sementeira foi determinada a partir da medição do comprimento do mesocótilo do milho após a emergência da cultura. A média, desvio padrão, coeficiente de variação e correlação dos valores de resistência ao rompimento do solo e profundidade de sementeira foram determinados. Os resultados demonstraram que independentemente da pratica cultural utilizada se verificou uma correlação negativa entre os valores encontrados para a resistência ao rompimento do solo e os valores de profundidade de deposição da semente, sendo o valor mais acentuado na condição de sementeira direta (r = - 0.77) à velocidade de trabalho de 4 km-1. A melhor performance de sementeira foi conseguida em sementeira direta com o aumento de velocidade de trabalho de 4 para 6 km-1 com um coeficiente de variação de 10.1%. A análise de variância demonstrou haverem diferenças significativas nas profundidades de sementeira causadas pela resistência do solo e pela velocidade de trabalho do semeador de sementeira direta

Does intake of bread supplemented with wheat germ have a preventive role on cardiovascular disease risk markers in healthy volunteers? A randomised, controlled, crossover trial

Objective Intake of whole grains is associated with a reduced risk of cardiovascular disease (CVD). This evidence is also strong for bran alone, but findings about germ are conflicting. Our aim was to elucidate the role of germ in primary prevention of cardiovascular events, and therefore, a staple food was selected for 6g of germ supplementation. This corresponds to sixfold increase in the global mean consumption of germ, while preserving the sensory proprieties of refined bread which is crucial for consumers acceptance. Design Randomised, double-blinded, crossover, controlled clinical trial with 15-week follow-up comprising a 2-week run-in, two intervention periods of 4weeks each and a 5-week washout period. Setting A single centre in the north of Portugal. Participants 55 eligible healthy adults (mean age of 34 years and body mass index between 19 and 38kg/m2) were randomly assigned. Interventions The study consisted of two intervention periods including daily intake of refined wheat bread enriched with 6g of wheat germ and control (non-enriched bread). Outcomes Changes in fasting cholesterol and triglycerides, fasting and postprandial glucose, insulin sensitivity and C reactive protein. Results We observed no significant effect of daily intake of wheat germ on cholesterol and triglycerides levels, on postprandial glucose response and on insulin sensitivity. Incremental area under curve glucose and homeostasis model assessment for insulin resistance did not change, suggesting that 6g of wheat germ have no effect on glucose metabolism. No effect was also observed in the subgroup of participants who complied with the protocol (n=47). Conclusions The absence of alterations on lipid and glucose profiles suggests that germ up to 6g/day may have no preventive effect on CVD risk. However, it is important to investigate other food vehicles that can accommodate higher doses of wheat germ in future studies.This work was supported by the Programa Operacional Competitividade e Internacionalização–COMPETE2020 and by National Funds through Fundação para a Ciência e a Tecnologia-FCT (through the operations FCOMP-01-0202-FEDER-038861 and POCI-01-0145-FEDER-007746).info:eu-repo/semantics/publishedVersio

Taxonomic, structural diversity and carbon stocks in a gradient of island forests

Assessment of forest ecosystems and their services is seen as a key action for the advancement of biodiversity objectives, and to inform the development and implementation of related policies and planning. The Azorean forest is important for timber production, the protection of soil and water resources, and for its recreational and aesthetic value. However, its role in carbon accumulation has not been fully addressed. We assessed plant diversity, forest structure and carbon stocks in a gradient of three forest types (Natural Forest-NF; Exotic Woodland-EW and Production Forest-PF) in three of the Azores islands. We used biodiversity indices and found that NF harboured the highest plant diversity levels and PF the lowest. Diversity levels were lower for structural than for taxonomic data, particularly for PF. The highest tree carbon stock was found at EW in one of the islands, while PF consistently exhibited relatively high tree carbon stocks in the three islands. The largest soil carbon stocks were found at EW, while leaf litter carbon stocks were higher at PF. We concluded that NF play a fundamental role as plant diversity hotspots but have lower relevance as carbon stocks what might be associated with montane environmental conditions. PFs provide economic assets and act as carbon sinks, while EWs play a major role as carbon sinks in soil, but also at tree level in the oldest forests.This research was funded by Project FOREST-ECO2-Towards an Ecological and economic valorization of the Azorean Forest ACORES-01-0145-FEDER-000014-Azores 2020 PO, 2016-2019; FEDER funds through the Operational Programme for Competitiveness Factors-COMPETE and by National Funds through FCT-Foundation for Science and Technology under the UID/BIA/50027/2019 and POCI-01-0145-FEDER-006821info:eu-repo/semantics/publishedVersio

Neuromuscular assessment of a stand-up paddle stroke

his study analyzed muscle activity during the stand-up paddle stroke, considering the paddling side and the adjacent and opposing muscles relative to the position of the arms during paddling. Methods: Fourteen male paddleboarders performed three trials covering 195 m in which surface electromyography of the upper trapezius, biceps brachii, triceps brachii, tibialis anterior, and gastrocnemius medialis were recorded (four-cycle strokes on each side). The data were processed according to percentage of maximum voluntary contraction (% MVC). The MVC activation values (µV) for each muscle were then calculated and presented as percentage MVC (% MVC). Results: The recovery phase accounted for 60% of the paddle cycle, while the pull phase represented 39%. During right-side paddling, higher % MVC was found in the opposite-side upper trapezius (24.35%, p < 0.01) during the pulling phase and in the adjacent biceps brachii (8.36%, p < 0.03) during the recovery phase. In left-side paddling, greater % MVC was found in the opposite-side upper trapezius (27.60%, p < 0.01) during the pulling phase and in the opposite-side triceps brachii (42.25%, p < 0.04) during the recovery phase. Furthermore, the pulling phase exhibited higher MVC in the opposite-side upper trapezius compared to the recovery phase, both in the right-side (24.35%, p < 0.03) and left-side (27.60%, p < 0.01) paddling. Conclusions: these findings help establish the muscular activity of both sides of the paddling technique and the differences between the upper and lower limbs.info:eu-repo/semantics/publishedVersio

Daily intake of wheat germ-enriched bread may promote a healthy gut bacterial microbiota: a randomised controlled trial

Wheat bran fibre has a beneficial effect on gastrointestinal function, but evidence for wheat germ is scarce. Accordingly, we evaluated the effects of daily intake of wheat germ on gastrointestinal discomfort and gut microbiota by adding wheat germ to refined (white) wheat bread, the most consumed bread type. We hypothesised that an improvement in the composition of refined bread could beneficially affect intestinal health without compromising consumers' acceptance.AM-R, CC, and LFA were responsible for the study design, carrying out the trial, data analysis, manuscript writ-ing, and final revision. CM, HP, SN and DS were partially responsi-ble for the data collection and analysis. JAT collaborated in the study design and final revision of the manuscript. AM-R and LFA were responsible for the statistical analysis. CC and LFA were coordina-tors of the project, with an equal contribution. This work is part of the VALORINTEGRADOR project, and was supported by the Programa Operacional Competitividade e Internacionalização and by National Funds through the Fundação para a Ciência e a Tecnologia (through the operations FCOMP-01-0202-FEDER-038861 and POCI-01-0145-FEDER-007746). Our thanks to Germen and Padaria Ribeiro for the production of the bread used in this trial. We would like to thank Fran-cisca Mendes for her assistance in laboratory work. We are also espe-cially grateful to all participants for their enthusiastic collaboration in this study. We extend our thanks to Milaydis Sosa-Napolskij for proof reading this manuscript.info:eu-repo/semantics/publishedVersio

Enhanced dietary formulation to mitigate winter thermal stress in gilthead sea bream (Sparus aurata): a 2D-DIGE plasma proteome study

Low water temperatures during winter are common in farming of gilthead sea bream in the Mediterranean. This causes metabolic disorders that in extreme cases can lead to a syndrome called "winter disease." An improved immunostimulatory nutritional status might mitigate the effects of this thermal metabolic stress. A trial was set up to assess the effects of two different diets on gilthead sea bream physiology and nutritional state through plasma proteome and metabolites. Four groups of 25 adult gilthead sea bream were reared during winter months, being fed either with a control diet (CTRL) or with a diet called "winter feed" (WF). Proteome results show a slightly higher number of proteins upregulated in plasma of fish fed the WF. These proteins are mostly involved in the immune system and cell protection mechanisms. Lipid metabolism was also affected, as shown both by plasma proteome and by the cholesterol plasma levels. Overall, the winter feed diet tested seems to have positive effects in terms of fish condition and nutritional status, reducing the metabolic effects of thermal stress

Ciência Cidadã em Portugal: no cruzamento entre diversas áreas do conhecimento

Nas últimas décadas, cidadãos entusiastas, cientistas amadores, educadores e cientistas profissionais começaram, com maior frequência, a colaborar conjunta e ativamente em programas de recolha de dados científicos para responder a questões-chave da investigação contemporânea. Esta intensa colaboração e participação, conhecida como ciência cidadã, é considerada uma tendência emergente, no entanto, exemplos desta prática remontam, pelo menos, ao século XIX. Em Portugal, apesar da sua aplicabilidade nas diversas áreas do conhecimento, a ciência cidadã é ainda pouco comum e praticamente desconhecida da comunidade científica. Pretende-se, assim, traçar o panorama da ciência cidadã em Portugal, mostrando exemplos de práticas nesta área desde o passado ao presente e as áreas do conhecimento nas quais se desenvolvem projetos de ciência cidadã. Serão também apresentados e discutidos resultados de um estudo preliminar sobre a perceção da comunidade científica nacional relativamente a esta prática.info:eu-repo/semantics/publishedVersio

Architecture of cooperation for multi-robot systems

This paper presents a new architecture for coordinating multiple autonomous robots in the execution of cooperative tasks. The architecture is based on the definition of a strategy that uses different tactics, setplays and roles. Roles enable to configure individual behaviour by performing specific tasks using a given set of actions. The proposed architecture allows flexible and efficient multi-robot operation in dynamic environments. The paper also presents an application of the proposed architecture to a complex domain such as middle-size robotic soccer. Our architecture is also generally enough to be applied in different robotic soccer leagues and similar multi-robot problems. Copyright © 2006 IFAC

Joint use of data and modeling in coastal wave transformation

In the framework of a research project entitled "BRISA-BReaking waves and Induced SAnd transport", a methodology was devised to characterize the waves joining together in-situ measurements and numerical wave propagation models. With this goal in mind, a number of in-situ measurements were made, for selected positions in front of Praia de Faro (South Portugal), during four days (25th to 28th March, 2009) by using different types of equipments (e.g., resistive wave gauges, pressure sensors, currentmeters and a new prototype pore pressure sensor using optical fibre). Wave records were obtained simultaneously offshore (at a water depth of 11.7 m below mean sea level, MSL) and at the surf and swash zones. The data processing and analysis were made by applying classical time domain techniques. Numerical simulations of the wave propagation between offshore and inshore for the measurement period were performed with two numerical models, a 1D model based on linear theory and a nonlinear Boussinesq-type model, COULWAVE, both forced by the measured offshore wave conditions of 27th March 2009. Comparisons between numerical results and field data for the pressure sensors placed in the surf and swash zones were made and discussed. This approach enables to evaluate the performance of those models to simulate those specific conditions, but also to validate the models by gaining confidence on their use in other conditions.Science and Technology Foundation of the Ministry of Science, Technology and Higher Education, Portugal [SFRH/BPD/20508/2004]info:eu-repo/semantics/publishedVersio