Prevalência de anti-Forssman numa amostra de população portuguesa e a sua caracterização

Introdução Descoberto em 1911 por Frederick Forssman, a expressão do antigénio (Ag) Forssman (Fs) varia entre espécies, estando raramente presente nos eritrócitos humanos. Em 1987, três famílias inglesas sem qualquer relação entre si, foram identificadas com um fenótipo designado Apae que, mais tarde, foi classificado como o 31º grupo sanguíneo: FORS. Os anticorpos (Ac) anti-Fs têm ocorrência natural em humanos e podem ter implicações transfusionais e de transplante, uma vez que o Ag está presente na superfície de eritrócitos, fluídos corporais e órgãos. Objetivos O principal objetivo deste trabalho foi avaliar a prevalência de Ab anti-Fs e esclarecer o seu impacto na medicina transfusional, classificando o tipo de imunoglobulina (Ig) envolvida. Materiais e Métodos Neste estudo foi utilizada a técnica standard de tubo para avaliar a presença de Ac anti-Fs em amostras de plasma de uma população de dadores Portugueses e classificar as imunoglobulinas envolvidas. Foi utilizada, para a realização de todas as experiências, uma suspensão de eritrócitos de ovelha a 3-5% com expressão positiva para Ag Fs. Resultados De um total de 11877 amostras, 117 (0,99%) apresentaram reações fracas (entre 0 e 1 numa escala de 0 a 4) quando em contacto com o Ag Fs presente na suspensão de eritrócitos de ovelha. Essas amostras foram posteriormente estudadas quanto à presença da mutação no gene GBGT1, responsável pela expressão da enzima Fs sintetase. Das 192 amostras estudadas (50 de cada grupo sanguíneo, à exceção do AB – o mais raro), para classificar o Ac envolvido, 52% revelaram ser apenas IgM, sendo as demais, uma mistura entre IgG e IgM. Conclusão Na população estudada, poucas amostras (<1%) apresentaram reação negativa contra os eritrócitos de ovelhas, confirmando a baixa prevalência deste grupo sanguíneo. Os padrões de reação das amostras são independentes do grupo sanguíneo ABO e do género. O Ac anti-Fs é principalmente, mas não exclusivamente, IgM.Introduction Discovered in 1911 by Frederick Forssman, the Forssman (Fs) antigen (Ag) expression varies among species, being rarely present on human red blood cells (RBC). In 1987, three unrelated English families were identified with a phenotype designed Apae which was later classified as the 31st blood group: FORS. The antibodies (Ab) anti-Fs have natural occurrence in humans and can interfere in transfusion and transplantation once the Ag is present on the surface of RBC, body fluids and organs. Goals The main goal of this research was to evaluate the prevalence of anti-Fs Ab and clarify its impact on transfusion medicine by classifying the type of immunoglobulin (Ig) involved. Materials and Methods In this study, standard tube technique was used to evaluate the presence of Ab anti-Fs in plasma samples from a Portuguese donor population and classify the immunoglobulin involved. It was used a 3-5% RBC sheep suspension with a positive expression for Fs Ag to perform all the experiments. Results From a total of 11877 samples, 117 (0,99%) showed weak reactions (between 0 and 1 on a scale from 0 to 4) when in contact with the Fs Ag present in the sheep RBC suspension. These samples would be further studied for the search of the mutation in the GBGT1 gene responsible for the expression of Fs synthase. From the 192 samples studied (50 from each blood type, except AB – the rarest) to classify the Ab involved, 52% revealed to be only IgM, being the remaining samples a mixture between IgG and IgM. Conclusion In the population studied, only a few samples (<1%) revealed a negative reaction against the sheep RBC, thus confirming the low prevalence of this blood group. The reaction patterns in the samples are independent of the ABO blood group and gender. The Ab against Fs is mainly, but not exclusively, IgMinfo:eu-repo/semantics/publishedVersio

El modelo de evaluación de competencias del prácticum en educación social basado en e-porfolio y rúbrica

La nueva concepción de la formación superior exige realizar parte de la formación inicial en contextos reales de trabajo para la cualificación de los futuros profesionales. En esta comunicación vamos a presentar una propuesta formativa y evaluativa del Prácticum I de Educación Social, pero concretamente, en el ámbito profesional de Menores. A través del porfolio electrónico, el alumno recogerá el trabajo realizado durante el Prácticum I, a través de la propuesta realizada por el profesor tutor. Para evaluar el porfolio electrónico y las actividades realizadas se emplearán rúbricas

PREVENÇÃO E DIAGNÓSTICO DE PATOLOGIAS ASSOCIADAS À TIREÓIDE

Os hormônios tireoideanos (HT) exercem funções importantes no crescimento, desenvolvimento e metabolismo de todos os vertebrados, a base para esse progresso é dada pelo hormônio triiodotironina (T3). Os HT são secretados constantemente pela glândula tireóide que secreta tiroxina (T4) da qual provém, por desiodação, grande parte do T3 circulante. A interação entre os HT e os receptores nucleares refletem nos efeitos biológicos desses hormônios que se apresentam em regiões específicas do DNA, assim determinam a ativação ou inibição de seus genes-alvo e o controle da síntese de proteínas específicas. Algumas patologias podem ser associadas aos hormônios tireoideanos como o hipotireoidismo (chamado de mixedema, em casos agravados) comumente resulta da deficiência de iodo, a qual acarreta em uma produção diminuída de HT, apresentando então manifestações clínicas padrões como: fraqueza muscular, bradicardia, depressão, déficit cognitivo e baixo índice metabólico. E hipertireoidismo ou tireotoxicose que é caracterizado pelo aumento de produção dos HT que acelera o metabolismo do paciente. O objetivo deste estudo é revisar na literatura as principais doenças relacionadas aos hormônios tireoideanos e as principais patologias associadas a esses hormônios. O presente trabalho trata-se de estudo bibliográfico do tipo exploratório-descritivo, cujas bases de dados utilizadas foram SciELO, BVS e PUBMED, sendo que para a busca dos artigos foram usados os descritores em saúde com palavras-chaves em inglês, português e espanhol: hormônios tireoideanos, T3, T4, prevenção, diagnóstico. A pesquisa teve como critério de inclusão cinco artigos que abordavam sobre o tema, no período de 2014 a 2019, baseado na avaliação de títulos, resumos e o artigo na íntegra; e como critério de exclusão, os que não atendiam aos objetivos do estudo. A medida que o projeto foi sendo desenvolvido notou-se a importância do conhecimento dos hormônios e suas possíveis doenças para então acontecer uma prevenção associada a uma boa qualidade de vida, não realizar o uso de cigarro, como também há prevenções que são indicadas para o hipotireoidismo e não para o hipertireoidismo, como a atividade física regular. Também é de suma importância que pacientes com mais de 40 anos realizem a dosagem de TSH com regularidade

Effects of a personalized intervention program on the biochemical and hematological profile in community dwelling old adults-the AGA@4life intervention model

Aging is a social and economic challenge of the highest importance and a multidisciplinary intervention seems to be a promising approach for improving the quality of life of elderly individuals. This project was designed aimed at promoting an active and healthy aging through the implementation of an intervention program based on the comprehensive geriatric assessment model (AGA@4life), focused on promoting health and wellbeing, independence and autonomy, mobility, and social inclusion. A non-randomized interventional study was designed to evaluate the effect of only a dietetic and nutritional approach (control group (CG)) and the combination of a tailored exercise program and a dietetic and nutritional approach (intervention group (IG)) in the biochemical and hematological profile of older adults in the framework of AGA@4life. The 34 participants enrolled, aged 65 years or over, were subject to a thorough baseline (T0) multidisciplinary diagnostic evaluation, including the gathering of clinical information and a battery of biochemical and hematological determinations, and reevaluated after eight weeks of intervention (T1). Between T0 and T1, an increase in albumin and total proteins serum levels were observed in both groups (p < 0.01); the hematological profile in CG and IG showed an increase in red cell count and hemoglobin (p < 0.05). In IG, an increase of HDL cholesterol (p < 0.001) and a decrease of triglycerides (p = 0.001) were still observed. The AGA@4life multidisciplinary intervention improved the hematological and biochemical profile of old adults, potentially contributing to delay the development of several aging comorbidities and increase the quality of life of participants.info:eu-repo/semantics/publishedVersio

Multimodality Imaging in Carotid Web

Purpose: Carotid web (CaW) is an underrecognized cause of cryptogenic stroke in young patients. The optimal imaging for CaW is unknown. We aim to evaluate the diagnostic accuracy of diverse imaging modalities for the diagnosis of CaW.Methods: Retrospective analysis of institutional neurovascular database was performed to identify patients with multimodal (CT angiogram–CTA, digital subtraction angiogram–DSA, and/or ultrasound–US) imaging diagnosis of CaW or atherosclerosis. Baseline clinical demographics were recorded. Blinded image analysis was performed for each imaging modality by separate readers. Discrepancies were settled by consensus. Two-sided Cohen's Kappa (κ) coefficient was used to evaluate the inter-rater agreement for the etiological diagnosis between imaging modalities.Results: Thirty patients/60 carotids were evaluated by CTA and 55 carotids were included. Patients with symptomatic CaW (n = 20), compared to individuals with atherosclerosis (n = 10), were younger (49 ± 9 vs. 60 ± 8 years; p &lt; 0.01), more commonly female (75% vs. 30%; p = 0.01), and less frequently presented vascular risk factors: Hypertension (40% vs. 100%; p &lt; 0.01), hyperlipidemia (0% vs. 50%; p &lt; 0.01), diabetes (10% vs. 40%; p = 0.05), and smoking (5% vs. 70%; p &lt; 0.01). High inter-rater correlation strength existed for CTA (n = 55; κ = 0.88; p &lt; 0.0001) and DSA (n = 28; κ = 0.86, p &lt; 0.0001) readers for lesion diagnosis while US inter-rater agreement was lower (κ = 0.553; p = 0.001). Across modalities CTA and DSA shared very high strength of agreement (κ = 0.92; p &lt; 0.0001), compared to a less pronounced agreement between US and CTA (κ = 0.553; p = 0.001). The strength of correlation between DSA-CTA was significantly more robust as compared to US-CTA (Z = 3.58; p = 0.0003).Conclusion: CTA and DSA demonstrated comparable and superior performance as compared to US in the diagnosis of CaW

Sleep loss causes emotional dysregulations increasing depression and anxiety: a reciprocal relationship

Introduction: Good quality sleep is essential for good neurocognitive performance, mental and physical health. However, changes in society have led to a worsening of sleep quality, which is associated with the development of anxiety and depression. Objective: ​​Discuss and contribute for a better understanding of the association between sleep loss and anxiety and depression. Methodology: This article presents a narrative review of the literature based on the analysis of scientific articles published from 2014 to 2022, in PubMed, about sleep deprivation and its association with anxiety and depression. Two criterias were applied, titles that did not mention the thematic association between sleep deprivation and depression and anxiety and abstracts that did not address that theme. After the exclusion criterias, was obtained in total, 45 articles originally in English (including book chapters, guidelines and case reports) remained. Discussion: Sleep deprivation is associated with an increase in cortisol levels, a reduction in testosterone and serotonin levels, and an increase in inflammatory markers. In this context, this condition is related to symptoms of depression and anxiety. Due changes in the pattern of society, such as increased use of the internet and a greater workload, have led a large part of the population to develop problems in sleep quality. Addictionaly, it is known that good quality sleep is essential for good neurocognitive performance, mental and physical health. Conclusion: This study discussed the relation between sleep deprivation, depression and anxiety. It is added to its effect on good neurocognitive performance, mental and physical health. Overall, it was evidenced that there is an association of mechanisms between these disorders. In this context, there is an urgent need for more specific research and dissemination of data on the association of sleep deprivation and depression and anxiety

Outcomes of carotid artery stenting at a high-volume Brazilian interventional neuroradiology center

OBJECTIVES: Carotid artery stenting is an emerging revascularization alternative to carotid endarterectomy. However, guidelines have recommended carotid artery stenting only if the rate of periprocedural stroke or death is < 6% among symptomatic patients and < 3% among asymptomatic patients. The aim of this study is to evaluate and compare clinical outcomes of symptomatic and asymptomatic patients who had undergone carotid artery stenting as a first-intention treatment. METHOD: A retrospective analysis of patients who underwent carotid artery stenting by our interventional neuroradiology team was conducted. Patients were divided into two groups: symptomatic and asymptomatic patients. The primary endpoints were ipsilateral ischemic stroke, ipsilateral parenchymal hemorrhage and major adverse cardiac and cerebrovascular events at 30 days. The secondary endpoints included ipsilateral ischemic stroke, ipsilateral parenchymal hemorrhage, ipsilateral transient ischemic attack and major adverse cardiac and cerebrovascular events between the 1- and 12-month follow-ups. RESULTS: A total of 200 consecutive patients were evaluated. The primary endpoints obtained in the symptomatic vs. asymptomatic groups were ipsilateral stroke (2.4% vs. 2.7%, p = 1.00), ipsilateral parenchymal hemorrhage (0.8% vs. 0.0%, p = 1.00) and major adverse cardiac and cerebrovascular events (4.7% vs. 2.7%, p = 0.71). The secondary endpoints obtained in the symptomatic vs. asymptomatic groups were ipsilateral ischemic stroke (0.0% vs. 0.0%), ipsilateral parenchymal hemorrhage (0.0% vs. 0.0%), ipsilateral TIA (0.0% vs. 0.0%, p = 1.00) and major adverse cardiac and cerebrovascular events (11.2% vs. 4.1%, p = 0.11). CONCLUSIONS: In this retrospective study, carotid artery stenting was similarly safe and effective when performed as a first-intention treatment in both symptomatic and asymptomatic patients. The study results comply with the safety requirements from current recommendations to perform carotid artery stenting as an alternative treatment to carotid endarterectomy

Violencia política y conflictos sociales en América Latina

Parte I. Violencia, derechos humanos y criminalidad ; Conflictos y violencias en Colombia -- Derechos humanos y seguridad democrática en Colombia : ¿un equilibrio posible? -- Conflictividad, violencia y control social : saberes latinoamericanos contemporáneos -- Pensar la justicia de adolescentes desde el campo jurídico -- Parte II. Conflictos armados, étnicos, territoriales y por los recursos naturales -- El conflicto Mapuche y el estado de Chile : una reflexión sobre la violencia y la imagen de Chile en el bicentenario -- Entre la violencia política y social una revisión a la inmigración africana subsahariana occidental en Buenos Aires (1990-2010) -- Producción de nuevas espacialidades y cambios de autoridad. Interacción, globalización-conflicto armado-grupos étnicos -- Los recursos mineros y la protesta ciudadana en la Argentina de la última década -- Performance do crime : Componentes dramáticos e teatrais dos grandes roubos no Brasil.Editorial Universidad del Nort

Violencia política y conflictos sociales en América Latina

La violencia socio-económica como categoría de estudio en las ciencias sociales y humanas, ha venido adquiriendo creciente relevancia en América Latina. Los problemas y variables metodológicas asociadas a su investigación han sido abordados con mayor profundidad desde la sociología, el Derecho, la Ciencia política y la Psicología Social. Es por esto, que esta publicación se propone abordar la violencia socio-económica como una categoría de análisis e investigación interdisciplinaria, posibilitando la reflexión académica en torno a las dinámicas de construcción del poder, la institucionalidad y los movimientos sociales en América Latina.Introducción; Parte I. Violencia, derechos humanos y criminalidad. Conflicto y violencias en Colombia / Roberto González Arana, Ivonne Molinares Guerrero; Derechos humanos y seguridad democrática en Colombia: ¿un equilibrio posible? / César Barreira; Conflictividad, violencia y control social: saberes latinoamericanos contemporáneos / José Vicente Tavares-dos-Santos; Pensar la justicia de adolescentes desde el campo jurídico / Nilia Viscardi, Marcia Barbero; Parte ii. Conflictos: armados, étnicos, territoriales y por los recursos naturales. Aproximaciones teórico-conceptuales en torno al conflicto armado colombiano / Luis Fernando Trejos Rosero; El conflicto mapuche y el estado de Chile: una reflexión sobre la violencia y la imagen de Chile en el bicentenario / Loreto Correa V.; Entre la violencia política y social. Una revisión a la inmigración africana subsahariana occidental en Buenos Aires (1990-2010) / Mary Luz Estupiñán Serrano; Producción de nuevas espacialidades y cambios de autoridad. Interacción globalización-conflicto armado-grupos étnicos / Clara Inés Aramburo Siegert; Los recursos mineros y la protesta ciudadana en la Argentina de la última década / Orietta Favaro, Graciela Iuorno; Performances do crime: componentes dramáticos e teatrais dos grandes roubos no Brasil / Jania Perla Diógenes de Aquino

Relatório do Projeto Laboratório de Criação Artística

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