Certification process of small grain cereals BRC in France

In France, genetic resources are maintained in several Biological Resources Centres (BRC) spread all over the country. Most part of these BRC are involved in a quality management process. The development of quality system becomes a priority in regard of new notions (as collection of reference) and requirement at European and international levels.In this context, the French small grain cereals BRC engages itself in a certification process since March 2014. The certification's framework applied to principal missions of small grain cereals BRC:acquisition and regeneration.Each year new materials are introduced in collection for different purposes (scientific projects, evaluation networks, ie...). To be able to answer to Nagoya protocol requirement and also to be in agreement with European collection (AEGIS) philosophy, acquisition process has been clarified and standardized. Regeneration of small grain cereals genetic resources are realized at INRA Clermont-Ferrand with around 10% of the whole collection (≈ 27 000 acc.) characterized per year for primary descriptors. A part of these data is available online on SIReGal database (http://urgi.versailles.inra.fr/siregal).conservation: Active collection is maintained in a 100 m3 cold room at +4°C and 30% relative humidity. It is used to regeneration, multiplication, distribution, characterization and assessment. Base collection is maintained at -20°C in freezer as secure storage. Various tests concerning drying before storage and germination are done to improve conservation conditions. distribution: To improve access to small grain cereals collections, a work on data's curation and submission to SIReGal database has been done and it still in process. Around 4 300 samples have been distributed in 2014, mainly to researchers and breeders but also to farmers and associations. Terms and conditions of BRC have been formalized and a first survey of genetic resources' requesters was launched in autumn 2014 to better understand their expectations and to have a feedback on BRC services (seed quality, available information, delay, documentation …). Each proceeding has been well documented to clarify and improved our methodology

What is behind the management of small grain cereals Biological Resources Centre?

National audienc

What is behind the management of small grain cereals biological centre?

The BreedWheat project and more particularly Céréales Vallée, INRA and INRA Transfert, has organized the International Wheat Innovation Workshop (IWIW) the 16th and 17th November 2015 in Clermont-Ferrand, France. Message de bienvenueL’innovation variétale doit pouvoir profiter des progrès rapides des technologies de génotypage et de phénotypage. L’objectif de ce colloque international est de faire connaître à la communauté scientifique et semencière les principales avancées sur la génomique, le séquençage, la génétique, l’écophysiologie et la caractérisation des ressources génétiques du blé tendre. Nous proposons de le faire au travers de projets et d’équipes qui travaillent dans le cadre de grands projets nationaux focalisés sur le blé. De nouvelles pistes d’applications et de recherches peuvent apparaitre de la confrontation de ces expériences entre elles et avec la communauté des céréales.Une plage importante sera dédiée aux discussions, avec des tables rondes prévues après chaque session, permettant ainsi de favoriser le dialogue entre chercheurs académiques et privés, entre chercheurs des différents projets et entre ces projets et d’autres initiatives.Ce colloque organisé par le projet français BreedWheat (Projet Investissements d’Avenir), associe WISP (Projet LOLA britannique), proWeizen (Alliance allemande pour la recherche et la sélection), CRP WHEAT (programme challenge des instituts de recherche internationaux), l’IWGSC (consortium international de séquençage du blé) et la Wheat Initiative. Il présentera les résultats obtenus dans ces projets et certains programmes associés.L’inscription est gratuite mais obligatoire et ouverte prioritairement aux partenaires des projets et programmes précités. Les personnes non partenaires devront remplir un questionnaire sur leur expertise et intérêt majeur. Les présentations orales sont principalement limitées aux partenaires des projets mais la soumission de posters est possible et fortement encouragée.The small grain cereals Biological Resources Centre (BRC), part of INRA plant BRC Networks, gathers a collection of around 27 000 accessions. A major part of the preserved accessions are patrimonial genetic resources (landraces, breeding lines, elite lines and registered cultivars), including bread wheat, durum wheat and relatives species, barleys, triticales, oats, Aegilops sp. and ryes.BRC has defined two strands of activity: The first one is related to collection's management, from acquisition of accession to sample's availability which is certified since June 2015 under French standard NF S 96-90. BRC brings its practical expertise of agronomical BRC management to quality organisation AFNOR in order to adapt this French standard at ISO level. A part of the collection is available online on SIReGal ttp://urgi.versailles.inra.fr/siregal). Annually, around 4 200 samples are freely distributed with a Material Transfer Agreement (MTA). BRC is also implicated in European Cooperative Programme for Plant Genetic Resources Cereals Networks and through this in the definition of the European collection AEGIS.The second axis concerns diversity's valorisation of collections by involvement in research programs. A core collection of 372 bread wheat accessions was defined from BRC collection (Balfourier et al, 2007) which is now highly genotyped (more than 200 000 SNPs markers) and phenotyped for a hundred traits (Bordes et al, 2008). Today, BRC participates to two main French projects: PIA Breedwheat, in which 4 600 accessions of BRC wheat collection were described at molecular and phenotype levels and CASDAR Colnator program where 570 French barley accessions will be evaluated under 2 years. Finally, BRC is also involved with private breeders in the national networks for cereal crop genetic resources. At European level, BRC is involved in FP7 Whealbi project by providing part of accessions, participating to multisite evaluation network and developing a biorepository for wheat collection

Comparative Studies of Three Pairs of α- and γ‑Conjugated Folic Acid Derivatives Labeled with Fluorine-18

The folate receptor (FR) is upregulated in various epithelial cancer types (FR α-isoform), while healthy tissues show only restricted expression. FR-targeted imaging using folate radiopharmaceuticals is therefore a promising approach for the detection of FR-positive cancer tissue. Almost all folate-based radiopharmaceuticals have been prepared by conjugation at the γ-carboxylic functionality of the glutamate moiety of folic acid. In this work, three pairs of fluorinated α- and γ-conjugated folate derivatives were synthesized and their in vitro and in vivo properties compared. The syntheses of all six regioisomers were obtained in good chemical yields using a multistep synthetic approach including the highly selective Cu­(I)-catalyzed 1,3-dipolar cycloaddition. The radiosyntheses of the α- and γ-conjugated ¹⁸F-labeled folate derivatives were accomplished in moderate to good radiochemical yields, high radiochemical purities (>95%), and specific activities ranging from 25 to 196 GBq/μmol. In vitro, all folate derivatives showed high binding affinity to the FR-α (IC₅₀ = 1.4–2.2 nM). In vivo PET imaging and biodistribution studies in FR-positive KB tumor-bearing mice demonstrated similar FR-specific tumor uptake for both regioisomers of each pair of compounds. However, FR-unspecific liver uptake was significantly lower for the α-regioisomers compared to the corresponding γ-regioisomers. In contrast, kidney uptake was up to 50% lower for the γ-regioisomers than for the α-regioisomers. These results show that the site of conjugation in the glutamyl moiety of folic acid has a significant impact on the in vivo behavior of ¹⁸F-based radiofolates, but not on their in vitro FR-binding affinity. These findings may potentially stimulate new directions for the design of novel ¹⁸F-labeled folate-based radiotracers

Multiplicity dependence of light (anti-)nuclei production in p–Pb collisions at sNN=5.02 TeV

The measurement of the deuteron and anti-deuteron production in the rapidity range −1 < y < 0 as a function of transverse momentum and event multiplicity in p–Pb collisions at √sNN = 5.02 TeV is presented. (Anti-)deuterons are identified via their specific energy loss dE/dx and via their time-of- flight. Their production in p–Pb collisions is compared to pp and Pb–Pb collisions and is discussed within the context of thermal and coalescence models. The ratio of integrated yields of deuterons to protons (d/p) shows a significant increase as a function of the charged-particle multiplicity of the event starting from values similar to those observed in pp collisions at low multiplicities and approaching those observed in Pb–Pb collisions at high multiplicities. The mean transverse particle momenta are extracted from the deuteron spectra and the values are similar to those obtained for p and particles. Thus, deuteron spectra do not follow mass ordering. This behaviour is in contrast to the trend observed for non-composite particles in p–Pb collisions. In addition, the production of the rare 3He and 3He nuclei has been studied. The spectrum corresponding to all non-single diffractive p-Pb collisions is obtained in the rapidity window −1 < y < 0 and the pT-integrated yield dN/dy is extracted. It is found that the yields of protons, deuterons, and 3He, normalised by the spin degeneracy factor, follow an exponential decrease with mass number