INTERNET DAN PERUBAHAN SOSIAL: PEMANFAATAN INTERNET UNTUK ADVOKASI DAN DISEMINASI DALAM STUDI KASUS PENEGAKAN PENGGUNAAN MASKER OLEH KEMENKES DAN PIHAK KEPOLISIAN

Presiden Indonesia Ir. Joko Widodo menetapkan Kedaruratan Keputusan Presiden Nomor 11 Tahun 2020 dan bekerjasama dengan Kementerian Kesehatan Indonesia Direktorat 2020 kepada masyarakat tentang pemakaian masker oleh semua orang ketika berada di luar rumah dan Cuci Tangan Pakai Sabun (CTPS). Dalam mendukung aturan pemerintah, media sosial ikut berperan membantu pemerintah dalam menyebarkan informasinya kepada masyarakat sehingga masyarakat dapat mengikuti dan menjalankan aturan yang dibuat oleh pemerintah Indonesia. Penelitian ini berusaha menjawab bagaimana Kemenkes RI dan Pihak Kepolisian melakukan Advokasi dan Diseminasi dalam penggunaan masker kepada masyarakat serta apa saja pengaruh dari kewajiban penggunaan masker yang memberi pengaruh sosial kepada masyarakat? Penelitian ini merupakan jenis penelitian kualitatif menggunakan metode studi kasus yaitu suatu serangkaian kegiatan ilmiah yang dilakukan secara intensif, terinci dan mendalam tentang suatu program, peristiwa, dan aktivitas, baik pada tingkat perorangan, sekelompok orang, lembaga, atau organisasi untuk memperoleh pengetahuan mendalam tentang peristiwa. Adapun keseimpulan dalam penelitian ini ialah Advokasi dan diseminasi kemenkes dan kepolisian dalam penggunaan masker kepada masyarakat dilakukan melalui internet di antaranya website, youtube, media sosial, dan advokasi. Segala hal yang berkaitan dengan diseminasi penggunaan masker di unggah pada setiap media internet. Revolusi komunikasi yang terjadi akibat pandemik covid 19 yang melanda dunia saat ini membawa perubahan sosial di masyarakat dari proses komunikasi dimana dipandemi ini masyaraat harus menggunakan makser dimana yang sebelumnya tidak ada aturan untuk menggunakan masker dimanapun berada

Synthesis of unexpected glucosidase inhibitors and determination of transition states for glucopyranose interconversion

Over the past four decades, the study of glycosidase inhibitors has evolved from being a simple subject of research interest to the generation of molecules with medicinal properties. Besides, glycosidase inhibitors are also widely used as agrochemicals and therapeutic agents. Inspired by the abundance of irreversible inhibitors with cyclopropane rings in nature, we synthesized eight D-glucosides in order to study the active site residues of glucosidases. All compounds were synthesized from á-D-glucose in good yields. While the â-D-glucosides were shown to be good substrates for sweet almond â-glucosidase, we showed that the á-D-glucosides are tight binding but poor substrates for yeast á-D-glucosidase. For example, 1-(4-chlorophenylcyclopropyl)methyl-á-D-glucopyranoside binds with a Kd of 6.7 ìM. The synthesis of â-glucosidase inhibitors, in our second project, was conceptualized on the results obtained from the inhibition of yeast á-glucosidase by cyclopropanated valienamine derivative. Our â-analogue was synthesized from methyl á-D-glucose in 12 steps (4% overall yield). This compound was shown to inhibit almond â-glucosidase, albeit poorly, indicating that á- and â-glucosidases transition state structures are different. Molecules with spirocyclopropyl moieties, are known to be potent glucosidase inhibitors. The third project describes our attempts toward the synthesis of glucosidase inhibitors with spirocyclopropyl moieties. The starting material was methyl á-D-glucopyranoside and after successfully completing the first seven steps of the proposed reaction sequence (Figure 4.12), the project was terminated as an expected radical initiated cyclization reaction could not be effected successfully. The project was therefore not completed, although potential other synthetic routes could lead to the desired target compounds. Our last project was a collaborative initiative with Prof. V. L. Schramm and in this we describe the transition states for the mutarotation of glucose. We measured several kinetic isotope effects while the Schramm laboratories performed ab initio calculations. We showed that the transition state for the spontaneous glucopyranose interconversion most likely involves a single water molecule that act as a general-base catalysis