AAVR-Displaying Interfaces: Serotype-Independent Adeno-Associated Virus Capture and Local Delivery Systems.

Interfacing gene delivery vehicles with biomaterials has the potential to play a key role in diversifying gene transfer capabilities, including localized, patterned, and controlled delivery. However, strategies for modifying biomaterials to interact with delivery vectors must be redesigned whenever new delivery vehicles and applications are explored. We have developed a vector-independent biomaterial platform capable of interacting with various adeno-associated viral (AAV) serotypes. A water-soluble, cysteine-tagged, recombinant protein version of the recently discovered multi-AAV serotype receptor (AAVR), referred to as cys-AAVR, was conjugated to maleimide-displaying polycaprolactone (PCL) materials using click chemistry. The resulting cys-AAVR-PCL system bound to a broad range of therapeutically relevant AAV serotypes, thereby providing a platform capable of modulating the delivery of all AAV serotypes. Intramuscular injection of cys-AAVR-PCL microspheres with bound AAV vectors resulted in localized and sustained gene delivery as well as reduced spread to off-target organs compared to a vector solution. This cys-AAVR-PCL system is thus an effective approach for biomaterial-based AAV gene delivery for a broad range of therapeutic applications

Low Level Light Could Work on Skin Inflammatory Disease: A Case Report on Refractory Acrodermatitis Continua

Low level laser or light treatment on the various clinical condition is getting considerable attention now. However, there has been no report about the clinical effect of low level polarized polychromatic noncoherent light (LPPL) on the inflammatory skin disease. We experienced a case of acrodermatitis continua in a pregnant woman refractory to any conventional treatment including the most potent topical steroid. She was successfully treated with LPPL. LPPL could be a possible treatment modality producing substantial clinical result in inflammatory skin condition without any side-effect

Annular Leukocytoclastic Vasculitis in a Patient with Ulcerative Colitis

Leukocytoclastic vasculitis (LV) is characterized by neutrophilic invasion and fibrinoid necrosis along with endothelial enlargement in postcapillary venules. Annular appearance of LV (ALV) is rare, but it can be accompanied by several systemic diseases. One of these systemic diseases is ulcerative colitis (UC), a subgroup of inflammatory bowel disease. Only one case was previously reported in which ALV was associated with UC, and herein we present one more case. A 66-year-old woman presented with painful polycyclic erythema on both palms, which had been present for 4 days. She had suffered from UC for 5 years. The patient had no fever or other systemic symptoms, and histological examination demonstrated typical LV. 200 mg of oral dapsone was taken daily to rapidly reduce her symptoms and signs, and after 1 week all lesions resolved completely without any adverse events. ALV is not a distinct condition and it can appear in a broad range of small vessel vasculitides. Although ALV in patients with UC is a very rare combination, clinicians need to be aware of this possible association

A case of assisted reproductive therapy-induced erythema nodosum

Erythema nodosum is a common variant of panniculitis. It is characterized by tender erythematous nodule and plaque on the anterior aspect of the leg. The etiology is not fully understood. It may be associated with a variety of disorders, including infection, medication, autoimmune disorders, pregnancy, and malignancy. A 33-year-old Korean woman presented with 1 week history of painful erythematous plaques on both knees. She was 7 weeks pregnant with assisted reproductive therapy, and had been maintained on daily intramuscular progesterone injection for 4 weeks. Histological examination of the lesions revealed septal panniculitis without vasculitis. Two days after discontinuing progesterone injection, the symptoms and lesions started to resolve. Herein we present a case of erythema nodosum caused by progesterone injection for endometrial preparation.OAIID:oai:osos.snu.ac.kr:snu2011-01/102/2008000790/5SEQ:5PERF_CD:SNU2011-01EVAL_ITEM_CD:102USER_ID:2008000790ADJUST_YN:NEMP_ID:A079501DEPT_CD:801CITE_RATE:.531FILENAME:reproductive therapy e nodosum.pdfDEPT_NM:의학과SCOPUS_YN:NCONFIRM:

Treatment of Keratoacanthoma with 5% Imiquimod Cream and Review of the Previous Report

Keratoacanthoma (KA) is a benign epidermal tumor, characterized by rapid and abundant growth, a tendency toward spontaneous regression and histopathologic similarity to squamous cell carcinoma (SCC). Because KA can be easily misdiagnosed as SCC, surgery is considered the treatment of choice. Recently, regression of KAs following application of 5% imiquimod cream (Aldara®) has been reported. We present 4 cases of KA treated with topical imiquimod, applied 3 to 4 times a week. Obvious improvement was observed after 4 to 6 weeks of application and the lesions were almost cleared leaving scars after 9 to 11 weeks. These results show that topical imiquimod can be an effective option for the conservative management of KA as previously reported. We also suggest that lesions treated with imiquimod cream should be considered for biopsy to judge histopathological remission after 5 to 8 weeks of application to shorten the duration of the treatment

Two Cases of H2-Receptor Antagonist Hypersensitivity and Cross-Reactivity

H2-receptor antagonists, such as cimetidine, ranitidine and famotidine, are some of the most commonly prescribed medications for gastric acid-related disorders. These compounds are generally well-tolerated and anaphylactic reactions to them are rare. Here, we report two cases of H2-receptor antagonist-induced anaphylactic reactions: the first presented with sudden dyspnea, sneezing, urticaria, and swelling of the eyelids after ranitidine intake. The second presented with sudden severe urticaria, facial swelling, chest discomfort, dizziness, and hypotension. Possible cross-reactivity with other H2-receptor antagonists was assessed by oral challenge and skin tests. To date, only a few reports addressing cross-reactivity among H2-receptor antagonists have been published. We review the literature and summarize the data available on drug cross-reactivity in H2-receptor antagonist hypersensitivity

Photoattachment of thiolated DNA probes on SU-8 spin-coated Blu-ray disk surfaces for biosensing

[EN] A methodology to modify Blu-ray disk (BD) surfaces by spin-coating an SU-8 epoxy photoresist is evaluated, to create a hybrid material with new functionalities. The optical performance of the new film surface disk is analyzed. The light-mediated activation of epoxy-to-thiol chemistry is applied to the site-specific and covalent binding of thiol-ended oligonucleotide probes to the SU-8 disk surface. For the optimized hybridization conditions, a limit of detection of 1 pM is reached employing enzymatic development. PCR products, from a Salmonella typhimurium serotype, are detected at an amplicon concentration of 20 pM. The reading of the assays is performed using a commercial BD-player.This work has been funded by the projects FEDER CTQ2010-15943 (CICYT, Spain), PROMETEO 2010/008 (Generalitat Valenciana) and an interdisciplinary UPV PAID 05 - 10 grant.Peris Chanzá, EJ.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, Á. (2013). Photoattachment of thiolated DNA probes on SU-8 spin-coated Blu-ray disk surfaces for biosensing. Journal of Materials Chemistry B. 1(45):6245-6253. https://doi.org/10.1039/c3tb21026jS6245625314

Real scale evaluation of vibration mitigation of sub-ballast layers with added tyre-derived aggregate

[EN] This paper represents a final stage in the assessment of granular sub-ballast materials mixed with tyre-derived aggregate (TDA) without binder material. The objective is to evaluate such mixtures through a full-scale test under real traffic conditions. An experimental track with three 30-metre long sections was constructed: one section was built with conventional sub-ballast; and the other two sections were built with mixtures containing increasing rubber content. This track was then monitored using accelerometers. The results show a clear reduction in the acceleration peaks as rubber content increases. Moreover, the excited frequency bandwidth tends to become narrower and shifts to lower frequencies.The works presented in this paper are part of a research project (Project COMPOVIA) funded by the Spanish CDTI (Centro para el Desarrollo Tecnologico Industrial) and the CTA (Corporacion TecnolOgica de Andalucia). The authors also wish to thank ADIF for their collaboration during the measurements carried out in its facilities.Martínez Fernández, P.; Hidalgo Signes, C.; Villalba Sanchis, I.; Pérez Mira, D.; Insa Franco, R. (2018). Real scale evaluation of vibration mitigation of sub-ballast layers with added tyre-derived aggregate. Construction and Building Materials. 169:335-346. https://doi.org/10.1016/j.conbuildmat.2018.03.027S33534616

Massive Genomic Decay in Serratia symbiotica, a Recently Evolved Symbiont of Aphids

All vertically transmitted bacterial symbionts undergo a process of genome reduction over time, resulting in tiny, gene-dense genomes. Comparison of genomes of ancient bacterial symbionts gives only limited information about the early stages in the transition from a free-living to symbiotic lifestyle because many changes become obscured over time. Here, we present the genome sequence for the recently evolved aphid symbiont Serratia symbiotica. The S. symbiotica genome exhibits several of the hallmarks of genome evolution observed in more ancient symbionts, including elevated rates of evolution and reduction in genome size. The genome also shows evidence for massive genomic decay compared with free-living relatives in the same genus of bacteria, including large deletions, many pseudogenes, and a slew of rearrangements, perhaps promoted by mobile DNA. Annotation of pseudogenes allowed examination of the past and current metabolic capabilities of S. symbiotica and revealed a somewhat random process of gene inactivation with respect to function. Analysis of mutational patterns showed that deletions are more common in neutral DNA. The S. symbiotica genome provides a rare opportunity to study genome evolution in a recently derived heritable symbiont