98 research outputs found
Incidencia del contenido inicial de residuos en frutas sobre los aceites esenciales cítricos
Las exportaciones de productos cítricos tienen un alto impacto en la economía regional. A fin de adecuarse a la calidad exigida por los mercados internacionales debe efectuarse un adecuado manejo de la producción en todos sus aspectos. Los niveles aceptados de residuos de plaguicidas tienden a ser cada vez menores. La aplicación de plaguicidas tales como: O-fenilfenol, clorpirifos, imazalil, procloraz, bromopropilato, cipermetrina,y thiabendazole en los cultivos y/o empaques hace factible la presencia de residuos en las frutas, pudiendo consecuentemente persistir en los jugos y aceites esenciales. Se plantearon como objetivos evaluar los residuos presentes en las frutas y su evolución en el proceso de industrialización de aceites esenciales. Se aplicaron técnicas extractivas tales como, microextracción en fase sólida y método QuEChERS modificado. Las determinaciones e identificación de los analitos se efectuaron por cromatografía gaseosa con detectores de microcaptura de electrones, de nitrógeno-fósforo, espectrometría de masa y cromatografía líquida con arreglo de diodos y fluorescencia. Los datos estadísticos indicaron que las metodologías analíticas son lineales en el rango de concentraciones previsto, de adecuada precisión, alta sensibilidad y recuperación. Dado que existe efecto matriz, en todos los casos se adoptó el método de calibración con muestras adicionadas. Los resultados de tres temporadas de producción indicaron que los tipos de plaguicidas y los niveles detectados son muy variables, aún para un mismo analito, debido posiblemente a la diferencia y variabilidad de los lotes de frutas ingresadas. La carga inicial de residuos en la fruta disminuye aproximadamente un 40 a 50%, debido al proceso de lavado y cepillado en la línea. En aceite pulido los valores medios de residuos expresados en mg/kg presentaron diferencias significativas para los diferentes plaguicidas. Los valores de persistencia relativa en el aceite, respecto a los residuos en frutas post lavado, no superaron en su mayoría el 10%. Los niveles residuales en los aceites descerados variaron ligeramente respecto al pulido, posiblemente debido a las características fisicoquímicas de los analito, las cuales inciden en la partición de los mismos en las diferentes fases, y los valores de persistencia relativa no superaron el 15%. Siendo el O-fenilfenol el de mayor persistencia, tanto en aceite pulido como descerado. Podemos concluir que la mayor eliminación de los residuos se produce en el proceso de lavado y en menor porcentaje en el pulido y descerado debido a la partición de los analitos en las fases acuosa, oleosa y cerosa.Centro de Investigaciones del Medioambient
The effects of auditory enrichment on zebrafish behavior and physiology
Environmental enrichment is widely used to improve welfare and behavioral performance of animal species. It ensures housing of laboratory animals in environments with space and complexity that enable the expression of their normal behavioral repertoire. Auditory enrichment by exposure to classical music decreases abnormal behaviors and endocrine stress responses in humans, non-humans primates, and rodents. However, little is known about the role of auditory enrichment in laboratory zebrafish. Given the growing importance of zebrafish for neuroscience research, such studies become critical. To examine whether auditory enrichment by classical music can affect fish behavior and physiology, we exposed adult zebrafish to 2 h of Vivaldi’s music (65–75 dB) twice daily, for 15 days. Overall, zebrafish exposed to such auditory stimuli were less anxious in the novel tank test and less active, calmer in the light-dark test, also affecting zebrafish physiological (immune) biomarkers, decreasing peripheral levels of pro-inflammatory cytokines and increasing the activity of some CNS genes, without overt effects on whole-body cortisol levels. In summary, we report that twice-daily exposure to continuous musical sounds may provide benefits over the ongoing 50–55 dB background noise of equipment in the laboratory setting. Overall, our results support utilizing auditory enrichment in laboratory zebrafish to reduce stress and improve welfare in this experimental aquatic organism
A first constraint on basal melt-water production of the Greenland ice sheet
PROMICE is funded by the Geological Survey of Denmark and Greenland (GEUS) and the Danish Ministry of Climate, Energy and Utilities under the Danish Cooperation for Environment in the Arctic (DANCEA), and is conducted in collaboration with DTU Space (Technical University of Denmark) and Asiaq, Greenland.The Greenland ice sheet has been one of the largest sources of sea-level rise since the early 2000s. However, basal melt has not been included explicitly in assessments of ice-sheet mass loss so far. Here, we present the first estimate of the total and regional basal melt produced by the ice sheet and the recent change in basal melt through time. We find that the ice sheet’s present basal melt production is 21.4 +4.4/−4.0 Gt per year, and that melt generated by basal friction is responsible for about half of this volume. We estimate that basal melting has increased by 2.9 ± 5.2 Gt during the first decade of the 2000s. As the Arctic warms, we anticipate that basal melt will continue to increase due to faster ice flow and more surface melting thus compounding current mass loss trends, enhancing solid ice discharge, and modifying fjord circulation.Publisher PDFPeer reviewe
Effects of both ecdysone and the acclimation to low temperature, on growth and metabolic rate of juvenile freshwater crayfish Cherax quadricarinatus (Decapoda, Parastacidae)
Treatment outcomes of fixed-dose combination versus separate tablet regimens in pulmonary tuberculosis patients with or without diabetes in Qatar
Background: Tuberculosis is considered the second most common cause of death due to infectious agent. The
currently preferred regimen for treatment of pulmonary tuberculosis (PTB) is isoniazid, rifampin, pyrazinamide, and
ethambutol, which has been used either as separate tablets (ST) or as fixed-dose combination (FDC). To date, no
studies have compared both regimens in Qatar. We aim to evaluate the safety and effectiveness of FDC and ST
regimen for treating PTB, in addition to comparing safety and efficacy of FDC and ST regimens in patients with
diabetes treated for TB.
Methods: A retrospective observational study was conducted in two general hospitals in Qatar. Patients diagnosed
with PTB received anti-tuberculosis medications (either as FDC or ST) administered by the nurse. Sputum smears
were tested weekly. We assessed the time to negative sputum smear and incidence of adverse events among FDC
and ST groups.
Results: The study included 148 patients. FDC was used in 90 patients (61%). Effectiveness was not different
between FDC and ST regimens as shown by mean time to sputum conversion (29.9 ± 18.3 vs. 35.6 ± 23 days, p = 0.12).
Similarly, there was no difference in the incidence of adverse events, except for visual one that was higher in ST group.
Among the 33 diabetic patients, 19 received the FDC and had faster sputum conversion compared to those who
received ST (31 ± 12 vs. 49.4 ± 30.9 days, p = 0.05). Overall, diabetic patients needed longer time for sputum conversion
and had more hepatotoxic and gastric adverse events compared to non-diabetics.
Conclusion: ST group had higher visual side effects compared to FDC. FDC may be more effective in diabetic patients;
however, further studies are required to confirm such finding.PublishedN/
Design, synthesis and biological assessment of novel N-substituted 3-(phthalimidin-2-yl)-2,6-dioxopiperidines and 3-substituted 2,6-dioxopiperidines for TNF-α inhibitory activity
Eight novel 2-(2,6-dioxopiperidin-3-yl)phthalimidine EM-12 dithiocarbamates 9 and 10, N-substituted 3-(phthalimidin-2-yl)-2,6-dioxopiperidines 11-14 and 3-substituted 2,6-dioxopiperidines 16 and 18 were synthesized as tumor necrosis factor-α (TNF-α) synthesis inhibitors. Synthesis involved utilization of a novel condensation approach, a one-pot reaction involving addition, iminium rearrangement and elimination, to generate the phthalimidine ring required for the creation of compounds 9-14. Agents were, thereafter, quantitatively assessed for their ability to suppress the synthesis on TNF-α in a lipopolysaccharide (LPS)-challenged mouse macrophage-like cellular screen, utilizing cultured RAW 264.7 cells. Whereas compounds 9, 14 and 16 exhibited potent TNF-α lowering activity, reducing TNF-α by up to 48% at 30 μM, compounds 12, 17 and 18 presented moderate TNF-α inhibitory action. The TNF-α lowering properties of these analogs proved more potent than that of revlimid (3) and thalidomide (1). In particular, N-dithiophthalimidomethyl-3-(phthalimidin-2-yl)-2,6-dioxopiperidine 14 not only possessed the greatest potency of the analogs to reduce TNF-α synthesis, but achieved this with minor cellular toxicity at 30 μM. The pharmacological focus of the presented compounds is towards the development of well-tolerated agents to ameliorate the neuroinflammation, that is, commonly associated with neurodegenerative disorders, epitomized by Alzheimer's disease and Parkinson's disease
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Design and results of the ice sheet model initialisation experiments initMIP-Greenland: an ISMIP6 intercomparison
Earlier large-scale Greenland ice sheet sea-level projections (e.g. those run during the ice2sea and SeaRISE initiatives) have shown that ice sheet initial conditions have a large effect on the projections and give rise to important uncertainties. The goal of this initMIP-Greenland intercomparison exercise is to compare, evaluate, and improve the initialisation techniques used in the ice sheet modelling community and to estimate the associated uncertainties in modelled mass changes. initMIP-Greenland is the first in a series of ice sheet model intercomparison activities within ISMIP6 (the Ice Sheet Model Intercomparison Project for CMIP6), which is the primary activity within the Coupled Model Intercomparison Project Phase 6 (CMIP6) focusing on the ice sheets. Two experiments for the large-scale Greenland ice sheet have been designed to allow intercomparison between participating models of (1) the initial present-day state of the ice sheet and (2) the response in two idealised forward experiments. The forward experiments serve to evaluate the initialisation in terms of model drift (forward run without additional forcing) and in response to a large perturbation (prescribed surface mass balance anomaly); they should not be interpreted as sea-level projections. We present and discuss results that highlight the diversity of data sets, boundary conditions, and initialisation techniques used in the community to generate initial states of the Greenland ice sheet. We find good agreement across the ensemble for the dynamic response to surface mass balance changes in areas where the simulated ice sheets overlap but differences arising from the initial size of the ice sheet. The model drift in the control experiment is reduced for models that participated in earlier intercomparison exercises
Enhanced basal lubrication and the contribution of the Greenland ice sheet to future sea-level rise
We assess the effect of enhanced basal sliding on the flow and mass budget of the Greenland ice sheet, using a newly developed parameterization of the relation between meltwater runoff and ice flow. A wide range of observations suggest that water generated by melt at the surface of the ice sheet reaches its bed by both fracture and drainage through moulins. Once at the bed, this water is likely to affect lubrication, although current observations are insufficient to determine whether changes in subglacial hydraulics will limit the potential for the speedup of flow. An uncertainty analysis based on our best-fit parameterization admits both possibilities: continuously increasing or bounded lubrication. We apply the parameterization to four higher-order ice-sheet models in a series of experiments forced by changes in both lubrication and surface mass budget and determine the additional mass loss brought about by lubrication in comparison with experiments forced only by changes in surface mass balance. We use forcing from a regional climate model, itself forced by output from the European Centre Hamburg Model (ECHAM5) global climate model run under scenario A1B. Although changes in lubrication generate widespread effects on the flow and form of the ice sheet, they do not affect substantial net mass loss; increase in the ice sheet’s contribution to sea-level rise from basal lubrication is projected by all models to be no more than 5% of the contribution from surface mass budget forcing alone
Fun at Antarctic grounding lines: Ice-shelf channels and sediment transport
Meltwater beneath the polar ice sheets drains, in part, through subglacial conduits. Landforms created by such drainages are abundant in areas formerly covered by ice sheets during the last glacial maximum. However, observations of subglacial conduit dynamics under a contemporary ice sheet are lacking. We present results from ice-penetrating radar to infer the existence of subglacial conduits upstream of the grounding line of Roi Baudouin Ice Shelf, Antarctica. The conduits are aligned with ice-shelf channels, and underlain by esker ridges formed from sediment deposition due to reduced water outflow speed near the grounding line. In turn, the eskers modify localice flow to initiate the bottom topography of the ice-shelf channels, and create small surface ridges extending onto the shelf. Relict features on the shelf are interpreted to indicate a history of these interactions and variability of past subglacial drainages. Because ice-shelf channels are loci where intense melting occurs to thin an ice shelf, these findings expose a novel link between subglacial drainage, sedimentation, and ice-shelf stability. To investigate the role of sediment transport beneath ice sheets further, we model the sheet-shelf system ofthe Ekstömisen catchment, Antarctica. A 3D finite element model (Elmer/ICE) is used to solve the transients full Stokes equation for isotropic, isothermal ice with a dynamic grounding line. We initialize the model with surface topography from the TanDEM-X satellites and by inverting simultaneously for ice viscosity and basaldrag using present-day surface velocities. Results produce a flow field which is consitent with sattelite and on-site observations. Solving the age-depth relationship allows comparison with radar isochrones from airborne data, and gives information about the atmospheric/dynamic history of this sector. The flow field will eventually be used to identify potential sediment sources and sinks which we compare with more than 400 km of seismic profiles collected over the floating ice shelves and the grounded ice sheet
Continuum-mechanical, Anisotropic Flow model for polar ice masses, based on an anisotropic Flow Enhancement factor
A complete theoretical presentation of the Continuum-mechanical, Anisotropic
Flow model, based on an anisotropic Flow Enhancement factor (CAFFE model) is
given. The CAFFE model is an application of the theory of mixtures with
continuous diversity for the case of large polar ice masses in which induced
anisotropy occurs. The anisotropic response of the polycrystalline ice is
described by a generalization of Glen's flow law, based on a scalar anisotropic
enhancement factor. The enhancement factor depends on the orientation mass
density, which is closely related to the orientation distribution function and
describes the distribution of grain orientations (fabric). Fabric evolution is
governed by the orientation mass balance, which depends on four distinct
effects, interpreted as local rigid body rotation, grain rotation, rotation
recrystallization (polygonization) and grain boundary migration (migration
recrystallization), respectively. It is proven that the flow law of the CAFFE
model is truly anisotropic despite the collinearity between the stress deviator
and stretching tensors.Comment: 22 pages, 5 figure
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