Tratamento endovascular de estenose de artéria renal em rim transplantado

Introdução: a estenose de artéria renal transplantada (EART) é a complicação vascular mais frequente do transplante renal. O diagnóstico é realizado através da arteriografia e o tratamento consiste na angioplastia transluminal percutânea (ATP). Objetivo: avaliar o sucesso clínico, a patência primária e secundária da ATP, bem como a sobrevida dos pacientes após o tratamento endovascular. Métodos: análise retrospectiva dos pacientes com EART tratados por ATP no período de janeiro de 2008 a dezembro de 2016 no Hospital Universitário Walter Cantídio. Resultados: 28 pacientes foram tratados por EART através do método endovascular. A pressão diastólica reduziu de 91,62 mmHg para 76 mmHg, em média, após 6 meses do tratamento (p<0,05). Observou-se uma redução nos níveis de creatinina após 3, 6 e 24 meses da angioplastia (p=0,005). As taxas de patência primária em 1, 3, 6, 12 e 24 meses foram de 89,3% +/- 5,8%, 85,6% +/- 6,7%, 81,8% +/- 7,4%, 78,1% +/-7,9% e 68,9% +/- 9,3%, respectivamente. A taxa de patência secundária foi de 100%. A taxa de sobrevida em 1, 3, 6, 12 e 24 meses foi de 96,4% +/- 3,5%, 96,4% +/-3,5%, 92,6% +/- 5,1%, 92,6% +/- 5,1% e 83,8% +/- 7,5%, respectivamente. Conclusão: o tratamento endovascular de EART apresenta boas taxas de patências primária e secundária e é efetivo em restaurar e manter a função renal em dois anos

Selenylated Imidazo[1,2<i>-a</i>]pyridine Induces Cell Senescence and Oxidative Stress in Chronic Myeloid Leukemia Cells

Imidazo[1,2-a]pyridines (IPs) have been studied regarding drug development. The objective of this work was to evaluate the antileukemic capacity of IP derivatives by screening their ability as a pro-oxidant. IP derivatives were synthesized and oral bioavailability and toxicity were analyzed in silico. Redox screening was performed on human Kasumi, KG-1, K562, and Jurkat leukemia cells. The IP derivative and the most responsive leukemic cell were selected for cytotoxicity, cell proliferation, cell senescence, and oxidative stress assays. The predictive toxicity analysis showed a possible effect on the reproductive system, but without mutagenic, carcinogenic, or irritability effects. MRK-107 against K562 cells was the compound that showed the best redox profile. MRK-107 did not induce cell death in K562 and monocyte cells. However, this compound was able to decrease cell proliferation and increase cell senescence after 48 and 72 h. Furthermore, MRK-107 induced oxidative stress in K562 cells after 72 h, increasing lipid peroxidation and decreasing reduced glutathione (GSH) contents. This study demonstrated that MRK-107-induced senescence with the involvement of oxidative stress is a possible mechanism of action, addressing this compound as a potential antitumor drug against chronic myeloid leukemia

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved