El discurso pedag?gico en el marco de la pedagog?a por proyectos y su incidencia en la producci?n de la escritura

142 p. Recurso Electr?nicoEste informe muestra la investigaci?n que se desarroll? en la Instituci?n Educativa Colegio Militar Almirante Padilla, (Ubicado en San Mateo, Soacha - Cundinamarca). Parti? de la pregunta ?C?mo incide el discurso pedag?gico en la ense?anza de la escritura en los ni?os de grado tercero del Colegio Militar Almirante Padilla en el marco de la pedagog?a por proyectos? En un principio, se indagaron antecedentes respecto al problema. Despu?s se establecieron los fundamentos conceptuales para el desarrollo de la ense?anza de la producci?n escrita, luego se determin? el discurso del docente de Lengua Castellana y su incidencia en los procesos de los estudiantes durante la investigaci?n. Posteriormente se dise?? y aplic? la propuesta de un proyecto de aula enfocada en diez misiones. Esto permiti? favorecer en los estudiantes procesos de innovaci?n, investigaci?n, pensamiento cr?tico, trabajo colaborativo, interacci?n y creaciones a partir de la pr?ctica con base en sus intereses, estimulando el trabajo cooperativo, como tambi?n la formaci?n integral a partir de las fortalezas individuales de los estudiantes. Finalmente se evalu? el impacto que tuvo la intervenci?n de la propuesta metodol?gica a trav?s del an?lisis de rubricas con un doble prop?sito: Evidenciar los aciertos y dificultades de los estudiantes en la producci?n textual y analizar el discurso del profesor en relaci?n con los procesos de escritura que propone a los ni?os y ni?as. Como conclusi?n, se implement? el proyecto de aula con base en el discurso instruccional. Lo que permiti? observar el avance en los procesos de escritura de los estudiantes.This research Project shows the work developed in Instituci?n Educativa Colegio Militar Almirante Padilla. (The school is located in San Mateo, Soacha-Cundinamarca). It started with the question: How does the teacher ?talk influence the third grade students writing process at the Militar Almirante Padilla School, according to Pedagogy for Projects? framework? At the beginning, background about the research problem was inquired into. Afterwards, a conceptual framework about writing teaching was stablished. Then, the Spanish teacher-talk and its influence in the students? processes were determined. Later, a proposal about ten missions was designed and applied. This allowed students to promote processes of innovation, research, critical thinking, collaborative work, interaction and creation, based on their interests, stimulating cooperative work, as well as integral training, based on the individual strengths of the students. Finally, the impact of the intervention of the methodological proposal was evaluated through the analysis of rubrics with a double purpose: to evidence the successes and difficulties of the students in the textual production and to analyze the teacher-talk in relation to the writing processes that develops with children. As a conclusion, the classroom project was implemented based on instructional discourse. What allowed observing the advance in the writing processes of the students. Key words: teacher-talk, significant learning, knowledge, written production, pedagogy for projects

PENGARUH PROFITABILITAS DAN LEVERAGE TERHADAP RETURN SAHAM (Studi pada Perusahaan Pertambangan yang Terdaftar di Bursa Efek Indonesia Tahun 2015-2019)

Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh profitabilitas dan leverage terhadap return saham perusahaan pertambangan yang terdaftar di Bursa Efek Indonesia tahun 2015-2019. Profitabilitas diukur dengan return on assets (ROA) dan leverage diukur dengan debt to equity ratio (DER). Pada penelitian ini, populasi yang digunakan adalah perusahaan pertambangan yang terdaftar di Bursa Efek Indonesia pada tahun 2015-2019 yang berjumlah 34 perusahaan. Pengambilan sampel dilakukan dengan teknik purposive sampling untuk mendapatkan sampel yang representative sesuai dengan kriteria yang telah ditetapkan. Jumlah sampel yang didapat adalah sebanyak 31 perusahaan sektor pertambangan dengan periode penelitian 2015-2019 sehingga diperoleh sampel sebanyak 155 data. Metode yang digunakan untuk mengalisis data adalah metode analisis regresi linier berganda. Hasil penelitian yang telah dilakukan dengan menggunakan analisis regresi linier berganda menunjukkan bahwa secara parsial profitabilitas berpengaruh positif dan signifikan terhadap return saham perusahaan pertambangan yang terdaftar di Bursa Efek Indonesia tahun 2015-2019. Sedangkan, leverage berpengaruh negatif dan signifikan terhadap return saham perusahaan pertambangan yang terdaftar di Bursa Efek Indonesia tahun 2015-2019. Hasil penelitian secara simultan menunjukkan bahwa profitabilitas dan leverage berpengaruh positif dan signifikan terhadap return saham perusahaan pertambangan yang terdaftar di Bursa Efek Indonesia tahun 2015-2019. Kata Kunci : Profitabilitas, leverage, return saham

5-hydroxyindolacetic acid (5-HIAA), a main metabolite of serotonin, is responsible for complete Freund's adjuvant-induced thermal hyperalgesia in mice

<p>Abstract</p> <p>Background</p> <p>The role of serotonin (5-hydroxytrptamine, 5-HT) in the modulation of pain has been widely studied. Previous work led to the hypothesis that 5-hydroxyindolacetic acid (5-HIAA), a main metabolite of serotonin, might by itself influence pain thresholds.</p> <p>Results</p> <p>In the present study, we investigated the role of 5-HIAA in inflammatory pain induced by intraplantar injection of complete Freund's adjuvant (CFA) into the hind paw of mice. Wild-type mice were compared to mice deficient of the 5-HT transporter (5-HTT-/- mice) using behavioral tests for hyperalgesia and high-performance liquid chromatography (HPLC) to determine tissue levels of 5-HIAA. Wild-type mice reproducibly developed thermal hyperalgesia and paw edema for 5 days after CFA injection. 5-HTT-/- mice treated with CFA had reduced thermal hyperalgesia on day 1 after CFA injection and normal responses to heat thereafter. The 5-HIAA levels in spinal cord and sciatic nerve as measured with HPLC were lower in 5-HTT-/- mice than in wild-type mice after CFA injection. Pretreatment of wild-type mice with intraperitoneal injection of para-chlorophenylalanine (p-CPA), a serotonin synthesis inhibitor, resulted in depletion of the 5-HIAA content in spinal cord and sciatic nerve and decrease in thermal hyperalgesia in CFA injected mice. The application of exogenous 5-HIAA resulted in potentiation of thermal hyperalgesia induced by CFA in 5-HTT-/- mice and in wild-type mice pretreated with p-CPA, but not in wild-type mice without p-CPA pretreatment. Further, methysergide, a broad-spectrum serotonin receptor antagonist, had no effect on 5-HIAA-induced potentiation of thermal hyperalgesia in CFA-treated wild-type mice.</p> <p>Conclusion</p> <p>Taken together, the present results suggest that 5-HIAA plays an important role in modulating peripheral thermal hyperalgesia in CFA induced inflammation, probably via a non-serotonin receptor mechanism.</p

The history and evolution of the clinical effectiveness of haemophilia type a treatment: a systematic review.

First evidence of cases of haemophilia dates from ancient Egypt, but it was when Queen Victoria from England in the 19th century transmitted this illness to her descendants, when it became known as the "royal disease". Last decades of the 20th century account for major discoveries that improved the life expectancy and quality of life of these patients. The history and evolution of haemophilia healthcare counts ups and downs. The introduction of prophylactic schemes during the 1970s have proved to be more effective that the classic on-demand replacement of clotting factors, nevertheless many patients managed with frequent plasma transfusions or derived products became infected with the Human Immunodeficiency Virus (HIV) and Hepatitis C virus during the 1980s and 1990s. Recombinant factor VIII inception has decreased the risk of blood borne infections and restored back longer life expectancies. Main concerns for haemophilia healthcare are shifting from the pure clinical aspects to the economic considerations of long-term replacement therapy. Nowadays researchers' attention has been placed on the future costs and cost-effectiveness of costly long-term treatment. Equity considerations are relevant as well, and alternative options for less affluent countries are under the scope of further research. The aim of this review was to assess the evidence of different treatment options for haemophilia type A over the past four decades, focusing on the most important technological advances that have influenced the natural course of this "royal disease"

Induction of humoral immune response to multiple recombinant Rhipicephalus appendiculatus antigens and their effect on tick feeding success and pathogen transmission

BACKGROUND: Rhipicephalus appendiculatus is the primary vector of Theileria parva, the etiological agent of East Coast fever (ECF), a devastating disease of cattle in sub-Saharan Africa. We hypothesized that a vaccine targeting tick proteins that are involved in attachment and feeding might affect feeding success and possibly reduce tick-borne transmission of T. parva. Here we report the evaluation of a multivalent vaccine cocktail of tick antigens for their ability to reduce R. appendiculatus feeding success and possibly reduce tick-transmission of T. parva in a natural host-tick-parasite challenge model. METHODS: Cattle were inoculated with a multivalent antigen cocktail containing recombinant tick protective antigen subolesin as well as two additional R. appendiculatus saliva antigens: the cement protein TRP64, and three different histamine binding proteins. The cocktail also contained the T. parva sporozoite antigen p67C. The effect of vaccination on the feeding success of nymphal and adult R. appendiculatus ticks was evaluated together with the effect on transmission of T. parva using a tick challenge model. RESULTS: To our knowledge, this is the first evaluation of the anti-tick effects of these antigens in the natural host-tick-parasite combination. In spite of evidence of strong immune responses to all of the antigens in the cocktail, vaccination with this combination of tick and parasite antigens did not appear to effect tick feeding success or reduce transmission of T. parva. CONCLUSION: The results of this study highlight the importance of early evaluation of anti-tick vaccine candidates in biologically relevant challenge systems using the natural tick-host-parasite combination

A Petri net model for railway bridge maintenance

This article describes the application of the Petri net modelling approach to managing the maintenance process of railway bridges. The Petri net model accounts for the degradation, inspection and repair processes of individual bridge elements in investigating the effectiveness of alternative maintenance strategies. The times governing the degradation and repair processes considered are stochastic and defined by the appropriate Weibull distribution. The model offers a capability for modelling the bridge asset which overcomes the limitations in the currently used modelling techniques reported in the literature. The bridge model also provides a means of predicting the future asset condition as a result of adopting different maintenance strategies. The solution of the Petri net model is performed using a Monte Carlo simulation routine. The application of the model to a typical metal railway bridge is also presented in the article

Transcriptome profiling of grapevine seedless segregants during berry development reveals candidate genes associated with berry weight

Indexación: Web of Science; PubMedBackground Berry size is considered as one of the main selection criteria in table grape breeding programs. However, this is a quantitative and polygenic trait, and its genetic determination is still poorly understood. Considering its economic importance, it is relevant to determine its genetic architecture and elucidate the mechanisms involved in its expression. To approach this issue, an RNA-Seq experiment based on Illumina platform was performed (14 libraries), including seedless segregants with contrasting phenotypes for berry weight at fruit setting (FST) and 6–8 mm berries (B68) phenological stages. Results A group of 526 differentially expressed (DE) genes were identified, by comparing seedless segregants with contrasting phenotypes for berry weight: 101 genes from the FST stage and 463 from the B68 stage. Also, we integrated differential expression, principal components analysis (PCA), correlations and network co-expression analyses to characterize the transcriptome profiling observed in segregants with contrasting phenotypes for berry weight. After this, 68 DE genes were selected as candidate genes, and seven candidate genes were validated by real time-PCR, confirming their expression profiles. Conclusions We have carried out the first transcriptome analysis focused on table grape seedless segregants with contrasting phenotypes for berry weight. Our findings contributed to the understanding of the mechanisms involved in berry weight determination. Also, this comparative transcriptome profiling revealed candidate genes for berry weight which could be evaluated as selection tools in table grape breeding programs.http://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0789-

P-hydroxyphenylpyruvate, an intermediate of the Phe/Tyr catabolism, improves mitochondrial oxidative metabolism under stressing conditions and prolongs survival in rats subjected to profound hemorrhagic shock

The aim of this study was to test the effect of a small volume administration of p-hydroxyphenylpyruvate (pHPP) in a rat model of profound hemorrhagic shock and to assess a possible metabolic mechanism of action of the compound. The results obtained show that hemorrhaged rats treated with 2-4% of the estimated blood volume of pHPP survived significantly longer (p<0.001) than rats treated with vehicle. In vitro analysis on cultured EA.hy 926 cells demonstrated that pHPP improved cell growth rate and promoted cell survival under stressing conditions. Moreover, pHPP stimulated mitochondria-related respiration under ATP-synthesizing conditions and exhibited antioxidant activity toward mitochondria-generated reactive oxygen species. The compound effects reported in the in vitro and in vivo analyses were obtained in the same millimolar concentration range. These data disclose pHPP as an efficient energetic substrates-supplier to the mitochondrial respiratory chain as well as an antioxidant supporting the view that the compound warrants further evaluation as a therapeutic agent. © 2014 Cotoia et al