493 research outputs found
Temporal Expression Pattern Of The Insulin-like Growth Factor Ii And Fibroblast Growth Factor Transcripts In Vian Embryogenesis
In this study, the abundance of IGF-II and bFGF transcripts was estimated in the chicken embryos using the competitive RT-PCR analysis. Significant enhancements in the abundance of IGF-II mRNA were observed at stages HH1 and 5, and a new accumulation in these levels was observed at stage HH18 in comparison to the basal levels. The abundance of bFGF mRNA increased significantly at stages HH18 and 20, followed by an upregulation in the expression of these transcripts at stage HH26. These findings provided important information about the temporal expression pattern of IGF-II and bFGF transcripts in the whole chicken embryos during in ovo development.515949955Bass, J., Oldham, J., Sharma, M., Kambadur, R., Growth factors controlling muscle development (1999) Dom. An. Endocrinol, 17, pp. 191-197Borja, A.J.M., Zeller, R., Meyers, C., Expression of alternatively spliced bFGF coding exons and antisense mRNAs during chicken embryogenesis (1993) Dev. Biol, 157, pp. 110-118Castelli, R., Porro, F., Tarsia, P., The heparins and cancer: Review of clinical trials and biological properties (2004) Vas. Med, 9, pp. 205-213Chomczynski, P., Sacchi, N., Single step method of RNA isolation by acid guanidinium thiocyanatephenol chloroform extraction (1987) Analytical Biochem, 162, pp. 156-159Cohn, M.J., Izpisúa-Belmonte, J.C., Abud, H., Heath, J.K., Tickle, C., Fibroblast growth factors induce additional limb development from the flank of chick embryos (1995) Cell, 80, pp. 739-746Cook, R.D., Weisberg, S., Transforming a response variable for linearity (1994) Biometrika, 81, pp. 731-737Darling, D.C., Brickell, P.M., Nucleotide sequence and genomic structure of the chicken insulin-like growth factor-II (IGF-II) coding region (1996) Gen. Comp. Endocrinol, 102, pp. 283-287Denley, A., Cosgrove, L., Booker, G., Wallace, J., Forbes, B., Molecular interactions of the IGF system (2005) Cytokine Growth Factor Rev, 16, pp. 421-439Florini, J.R., Magri, K.A., Ewton, D.Z., James, P.L., Grindstaff, K., Rotwein, P., Spontaneous differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II (1991) J. Biol. Chem, 266, pp. 15917-15923Florini, J.R., Ewton, D.Z., Cooligan, S.A., Growth hormone and the insulin-like growth factor system in myogenesis (1996) Endocrine Rev, 1795, pp. 481-517Gabriel, J.E., Javiel, H.A., Alvares, L.A., Schmidt, G., Coutinho, L.L., In situ detection of the myogenic factor MyoD in whole chicken embryos (2000) Genet. Mol. Biol, 23, pp. 145-148Gabriel, J.E., Alvares, L.E., Gobet, M.C., de Paz, C.C.P., Packer, I.U., Macari, M., Coutinho, L.L., Expression of MyoD, myogenin, myostatin and Hsp70 transcripts in chicken embryos submitted to mild cold or heat (2003) J. Thermal Biol, 28, pp. 261-269Hamburger, V., Hamilton, H.L., A series of normal stages in the development of the chick embryo (1951) J. Morphol, 88, pp. 49-92Hannon, K., Smith, C.K., Bales, K.R., Santerre, R.F., Temporal and quantitative analysis of myogenic regulatory and growth factor gene expression in the developing mouse embryo (1992) Dev. Biol, 151, pp. 137-144Kocamis, H., Killefer, J., Expression profiles of IGF-I, IGF-II, bFGF and TGF-b2 growth factors during chicken embryonic development (2003) Turk J Vet Anim Sci, 27, pp. 367-372Kost, T.A., Theodorakis, N., Hughes, S.H., The nucleotide sequence of the chick cytoplasmic betaactin gene (1983) Nucleic Acids Res, 11, pp. 8287-8301Muramatsu, M., Yamada, M., Takai, S., Miyazaki, M., Suppression of basic fibroblast growth factor-induced angiogenesis by a specific chymase inhibitor, BCEAB, through the chymase-angiotensin-dependent pathway in hamster sponge granulomas (2002) Br. J. Pharmacol, 137, pp. 554-560Ohuchi, H., Nakagawa, T., Yamamoto, A., Araga, A., Ohata, T., Isbimam, Y., Yoshioka, H., Noji, S., The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor (1997) Development, 113, pp. 1419-1434Pirskanen, A., Kiefer, J.C., Hauschka, S.D., IGFs, insulin, Shh, bFGF, and TGF-beta 1 interact synergistically to promote somite myogenesis in vitro (2000) Dev. Biol, 224, pp. 189-203Pownall, M.E., Emerson Jr., C.J., Sequential activation of three myogenic regulatory genes during somite mophogenesis in quail embryos (1992) Dev. Biol, 151, pp. 67-79Sambrook, J., Fritsch, E.F., Maniatis, T. Extraction, purification, analysis of messenger RNA from eukaryotic cells. In: Ford, N. Molecular cloning: a laboratory manual. 2.ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989, pp.7.40-7.87Sanchez, C.L., Rodriguez-Gallardo, L., Alvarez, I.S., Climent, V., Garcia-Martinez, V., Effects of growth factors on the commitment of chick blastoderm (2001) Int. J. Dev. Biol, 45, pp. S109-S110SAS Institute. SAS/STAT User's guide. Online Version. 8.ed. Cary: SAS Institute, 1999Szebenyi, G., Fallon, W., Fibroblast growth factors as multifunctional signaling factors (1999) Int Rev Citol, 185, pp. 45-106Tsai, S., Wiltbank, M.C., Quantification of mRNA using competitive RT-PCR with standard curve methodology (1996) Biotechniques, 21, pp. 862-86
A comparative analysis of thermophysical properties correlations for n-paraffins to be used in wax precipitation modeling
The performance of a thermodynamic wax precipitation model strongly depends upon the n-paraffin thermophysical properties used. In order to estimate them, several correlations have been proposed, and their values have a great impact on both calculated wax disappearance temperature (WDT) and amount of wax precipitated at each temperature (WPC). The main goal of this work is to evaluate the correlations available for the relevant thermophysical properties aiming at achieving a reliable wax precipitation modeling. The methodology used involves the direct comparison of the correlations with the values of pure n-paraffin properties, and indirect evaluation by their use in the estimation of wax disappearance temperatures, the amount of wax precipitated at each temperature, and DSC experimental curves. This study contemplates two thermodynamic approaches for paraffin precipitation: the solid solution (SS), which considers the formation of one solid solution; and the multisolid phase model (MS), that assumes that each solid phase consists of a pure component.publishe
The one-dimensional XXZ model with long-range interactions
The one-dimensional XXZ model (s=1/2, N sites) with uniform long-range
interactions among the transverse components of the spins is considered. The
Hamiltonian of the model is explicitly given by
where the
are half the Pauli spin matrices. The model is exactly solved by
applying the Jordan-Wigner fermionization, followed by a Gaussian
transformation. In the absence of the long-range interactions (I=0), the model,
which reduces to the isotropic XY model, is known to exhibit a second-order
quantum phase transition driven by the field at zero temperature. It is shown
that in the presence of the long-range interactions (I different from 0) the
nature of the transition is strongly affected. For I>0, which favours the
ordering of the transverse components of the spins, the transition is changed
from second- to first-order, due to the competition between transverse and xy
couplings. On the other hand, for I<0, which induces complete frustration of
the spins, a second-order transition is still present, although the system is
driven out of its usual universality class, and its critical exponents assume
typical mean-field values.Comment: 5 pages, 1 figure, presented at ICM2000, to be published in the
Proceedings (Journal of Magnetism & Magnetic Materials
Photoinduced antibacterial activity of the essential oils from Eugenia brasiliensis lam and Piper mosenii C. DC. by blue led light
The objective of this work was to evaluate the phytochemical composition and the antibacterial and antibiotic-modulating activities of the essential oils of Eugenia brasiliensis Lam (OEEb) and Piper mosenii C. DC (OEPm) singly or in association with blue LED (Light-emitting diode) light. The antibacterial and antibiotic-modulatory activities of the essential oils on the activity of aminoglycosides were evaluated to determine the minimum inhibitory concentration (MIC, \u3bcg/mL) in the presence or absence of exposure to blue LED light. The chemical analysis showed \u3b1-pinene and bicyclogermacrene as major constituents of OEPm, whereas \u3b1-muurolol was the main compound of OEEb. Both OEEb and OEPm showed MIC 65 512 \u3bcg/mL against the strains under study. However, the association of these oils with the blue LED light enhanced the action of the aminoglycosides amikacin and gentamicin. In conclusion, the association of aminoglycosides with the blue LED light and essential oils was effective against resistant bacteria
Measurements of long-range near-side angular correlations in TeV proton-lead collisions in the forward region
Two-particle angular correlations are studied in proton-lead collisions at a
nucleon-nucleon centre-of-mass energy of TeV, collected
with the LHCb detector at the LHC. The analysis is based on data recorded in
two beam configurations, in which either the direction of the proton or that of
the lead ion is analysed. The correlations are measured in the laboratory
system as a function of relative pseudorapidity, , and relative
azimuthal angle, , for events in different classes of event
activity and for different bins of particle transverse momentum. In
high-activity events a long-range correlation on the near side, , is observed in the pseudorapidity range . This
measurement of long-range correlations on the near side in proton-lead
collisions extends previous observations into the forward region up to
. The correlation increases with growing event activity and is found
to be more pronounced in the direction of the lead beam. However, the
correlation in the direction of the lead and proton beams are found to be
compatible when comparing events with similar absolute activity in the
direction analysed.Comment: All figures and tables, along with any supplementary material and
additional information, are available at
https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-040.htm
Study of the production of and hadrons in collisions and first measurement of the branching fraction
The product of the () differential production
cross-section and the branching fraction of the decay () is
measured as a function of the beauty hadron transverse momentum, ,
and rapidity, . The kinematic region of the measurements is and . The measurements use a data sample
corresponding to an integrated luminosity of collected by the
LHCb detector in collisions at centre-of-mass energies in 2011 and in 2012. Based on previous LHCb
results of the fragmentation fraction ratio, , the
branching fraction of the decay is
measured to be \begin{equation*} \mathcal{B}(\Lambda_b^0\rightarrow J/\psi
pK^-)= (3.17\pm0.04\pm0.07\pm0.34^{+0.45}_{-0.28})\times10^{-4},
\end{equation*} where the first uncertainty is statistical, the second is
systematic, the third is due to the uncertainty on the branching fraction of
the decay , and the
fourth is due to the knowledge of . The sum of the
asymmetries in the production and decay between and
is also measured as a function of and .
The previously published branching fraction of , relative to that of , is updated.
The branching fractions of are determined.Comment: 29 pages, 19figures. All figures and tables, along with any
supplementary material and additional information, are available at
https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-032.htm
Evidence for the strangeness-changing weak decay
Using a collision data sample corresponding to an integrated luminosity
of 3.0~fb, collected by the LHCb detector, we present the first search
for the strangeness-changing weak decay . No
hadron decay of this type has been seen before. A signal for this decay,
corresponding to a significance of 3.2 standard deviations, is reported. The
relative rate is measured to be
, where and
are the and fragmentation
fractions, and is the branching
fraction. Assuming is bounded between 0.1 and
0.3, the branching fraction would lie
in the range from to .Comment: 7 pages, 2 figures, All figures and tables, along with any
supplementary material and additional information, are available at
https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-047.htm
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