553 research outputs found
Dry aerosol deposition over the North Sea estimated from aircraft measurements
A mathematical approach based on the Monin-Obukhov similarity theory is used to predict the wind speed, friction velocity and drag coefficient, which are then introduced in the well-known deposition model of Slinn and Slinn (1980), to calculate the dry deposition of heavy metals into the North Sea. This model is perfectly suitable for aircraft sampling considering the fact that usually due to safety reasons, flights at the reference height used in deposition models (10m), are not possible. To check this approach, deposition velocities were calculated based on the airborne concentrations of Cu, Cd, Zn and Pb obtained by sampling with the aid of an aircraft over the Dutch continental shelf of the North Sea. Results are in agreement with those found in the literature. A rough estimation of the atmospheric input for these heavy metals and comparison with riverine inputs and direct discharges is also included
Photosensitized electron transport across lipid vesicle walls: Enhancement of quantum yield by ionophores and transmembrane potentials
Enzymatic synthesis of vanillin
Due to increasing interest in natural vanillin, two enzymatic routes for the synthesis of vanillin were developed. The flavoprotein vanillyl alcohol oxidase (VAO) acts on a wide range of phenolic compounds and converts both creosol and vanillylamine to vanillin with high yield. The VAO-mediated conversion of creosol proceeds via a two-step process in which the initially formed vanillyl alcohol is further oxidized to vanillin. Catalysis is limited by the formation of an abortive complex between enzyme-bound flavin and creosol. Moreover, in the second step of the process, the conversion of vanillyl alcohol is inhibited by the competitive binding of creosol. The VAO-catalyzed conversion Of vanillylamine proceeds efficiently at alkaline pH values. Vanillylamine is initially converted to a vanillylimine intermediate product, which is hydrolyzed nonenzymatically to vanillin. This route to vanillin has biotechnological potential as the widely available principle of red pepper, capsaicin, can be hydrolyzed enzymatically to vanillylamine
Synthesis of medium-chain glycerides using lipase from Candida rugosa
Enzymatic synthesis of medium-chain glycerides (MCG) from capric acid and glycerol was studied using lipase from Candida rugosa. The effects of various reaction parameters such as time, molar ratio of substrates (mmol capric acid/mmol glycerol), amount of lipase, type of organic solvents, and initial water activity (a w ) were studied. The best conditions tested for MCG synthesis at 37°C were, respectively, time, 24 h; molar ratio of substrates, 2.5; and amount of lipase, 100.0 mg. The use of organic solvents greatly influenced the activity of lipase in the synthesis of MCG. Generally, activity of lipase was high in nonpolar solvents with log P values from 3.50 to 4.50, where P is the partition coefficient between water and 1-octanol. The enzymatic synthesis of MCG was preferably carried out at an initial a w of 0.328, which resulted in maximal yield. Analysis of the products of reaction using gas chromatography showed that lipase from Candida rugosa seemed to produce more dicaprin and tricaprin than monocaprin
Alcoholysis of palm oil mid-fraction by lipase from Rhizopus rhizopodiformis
A mycelial lipase from Rhizopus rhizopodiformis was prepared in fragment form. The lipase was examined to catalyze the alcoholysis of palm oil mid-fraction (PMF) in organic solvents. High percentage conversions of PMF to alkyl esters were achieved when methanol or propanol was used as acyl acceptor. Of the two most prevalent fatty acids in PMF, palmitic acid seemed to be preferred over oleic acid in the formation of methyl and propyl esters. The optimal ratio of oil to methanol in the alcoholysis reaction is 1 to 2 moles. The lipase exhibited high alcoholysis activities in nonpolar solvents (log P>2), such as hexane, benzene, toluene, and heptane. The enzyme showed exceptionally high thermostability
Composition and dynamics of the nucleolinus, a link between the nucleolus and cell division apparatus in surf clam (Spisula) oocytes
Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of American Society for Biochemistry and Molecular Biology for personal use, not for redistribution. The definitive version was published in Journal of Biological Chemistry 287 (2012): 6702-6713, doi:10.1074/jbc.M111.288506.The nucleolinus is a little-known
cellular structure, discovered over 150
years ago (1) and thought by some
investigators in the late 19th to mid-20th
century to function in the formation of the
centrosomes or spindle. A role for the
nucleolinus in formation of the cell division
apparatus has recently been confirmed in
oocytes of the surf clam, Spisula solidissima
(2). However, we know so little about the
composition and dynamics of this
compartment, it is difficult to construct
mechanistic hypotheses or even to be sure
that prior reports were describing
analogous structures in the cells of mammals, amphibians, plants, and other
organisms where it was observed. Surf
clam oocytes are an attractive model to
approach this problem because the
nucleolinus is easily visible by light
microscopy, making it accessible by laser
microsurgery as well as isolation by
common cell fractionation techniques. In
this report we analyze the macromolecular
composition of isolated Spisula nucleolini
and examine the relationship of this
structure to the nucleolus and cell division
apparatus. Analysis of nucleolinar RNA
and protein revealed a set of molecules that
overlaps with, but is nevertheless distinct
from the nucleolus. The proteins identified
were primarily ones involved in nucleic acid
metabolism and cell cycle regulation.
Monoclonal antibodies generated against
isolated nucleolini revealed centrosomal
forerunners in the oocyte cytoplasm.
Finally, induction of damage to the
nucleolinus by laser microsurgery altered
the trafficking of α- and γ-tubulin after
fertilization. These observations strongly
support a role for the nucleolinus in cell
division and represent our first clues
regarding mechanism.This work was supported by a grant from the NIH (GM088503) to M.C.A
A review on substances and processes relevant for optical remote sensing of extremely turbid marine areas, with a focus on the Wadden Sea
The interpretation of optical remote sensing data of estuaries and tidal flat areas is hampered by optical complexity and often extreme turbidity. Extremely high concentrations of suspended matter, chlorophyll and dissolved organic matter, local differences, seasonal and tidal variations and resuspension are important factors influencing the optical properties in such areas. This review gives an overview of the processes in estuaries and tidal flat areas and the implications of these for remote sensing in such areas, using the Wadden Sea as a case study area. Results show that remote sensing research in extremely turbid estuaries and tidal areas is possible. However, this requires sensors with a large ground resolution, algorithms tuned for high concentrations of various substances and the local specific optical properties of these substances, a simultaneous detection of water colour and land-water boundaries, a very short time lag between acquisition of remote sensing and in situ data used for validation and sufficient geophysical and ecological knowledge of the area. © 2010 The Author(s)
Essential versus accessory aspects of cell death: recommendations of the NCCD 2015
Cells exposed to extreme physicochemical or mechanical stimuli die in an uncontrollable manner, as a result of their immediate structural breakdown. Such an unavoidable variant of cellular demise is generally referred to as ‘accidental cell death’ (ACD). In most settings, however, cell death is initiated by a genetically encoded apparatus, correlating with the fact that its course can be altered by pharmacologic or genetic interventions. ‘Regulated cell death’ (RCD) can occur as part of physiologic programs or can be activated once adaptive responses to perturbations of the extracellular or intracellular microenvironment fail. The biochemical phenomena that accompany RCD may be harnessed to classify it into a few subtypes, which often (but not always) exhibit stereotyped morphologic features. Nonetheless, efficiently inhibiting the processes that are commonly thought to cause RCD, such as the activation of executioner caspases in the course of apoptosis, does not exert true cytoprotective effects in the mammalian system, but simply alters the kinetics of cellular demise as it shifts its morphologic and biochemical correlates. Conversely, bona fide cytoprotection can be achieved by inhibiting the transduction of lethal signals in the early phases of the process, when adaptive responses are still operational. Thus, the mechanisms that truly execute RCD may be less understood, less inhibitable and perhaps more homogeneous than previously thought. Here, the Nomenclature Committee on Cell Death formulates a set of recommendations to help scientists and researchers to discriminate between essential and accessory aspects of cell death
A budget approach applied to nutrients (N, P, Si) and DIC in the Southern Bight of the North Sea: results from the CANOPY project
Deliberate termination of life of newborns with spina bifida, a critical reappraisal
Objects: Deliberate termination of life of newborns (involuntary euthanasia) with meningomyelocele (MMC) is practiced openly only in the Netherlands. 'Unbearable and hopeless suffering' is the single most cited criterion for this termination, together with the notion that 'there are no other proper medical means to alleviate this suffering'. In this paper, both (and other) statements are questioned, also by putting them in a broader perspective. Methods: First, a historical overview of the treatment of newborns with MMC is presented, concentrating on the question of selection for treatment. Second, a thorough analysis is made of the criteria used for life termination. Third, a case of a newborn with a very severe MMC is presented as a 'reference case'. Conclusion: 'Unbearable and hopeless suffering' cannot be applied to newborns with MMC. They are not 'terminally ill' and do have 'prospects of a future'. In these end-of-life decisions, 'quality of life judgments' should not be applied. When such a newborn is not treated, modern palliative care always will suffice in eliminating possible discomfort. There is no reason whatsoever for active life-termination of these newborns
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