Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment

Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death

Effect of organic precursor in hybrid sol–gel coatings for corrosion protection and the application on hot dip galvanised steel

Sol-gel coating material with enhanced corrosion protection for zinc-coated steel has been obtained through the incorporation of mono-phenol and bi-phenol organic precursors in an epoxide functionalised-silica-zirconia matrix. The effect of the presence of the organic precursors in the baseline formulation has been studied; sol stability has been examined by viscosity evolution; gel densification stage has been studied by differential scanning calorimetry (DSC); material composition has been analysed by Fourier transform infrared spectroscopy (FTIR) and X-ray spectroscopy (XPS); coating thickness and roughness has been measured by profilometry. Corrosion performance in three artificial weathering tests showed outstanding performance in the delay of zinc and steel corrosion products emergence, and electrochemical impedance spectroscopy (EIS) measurement permitted the identification of the coating presenting the most promising properties in terms of corrosion protection. Developed coatings have shown outstanding contribution to service life extension of zinc-coated parts.The authors thank the support of the Basque Government for EMAITEK 2017 program and the ELKARTEK project NG-FAB16 (contract number KK2016-00025). The authors thank CIC BiomaGUNE and Luis Yate for XPS characterisation

Correspondencia personal recibida por Manuel José Mosquera

Correspondencia personal recibida por Manuel José Mosquera desde 1831 hasta 1853. Se destacan particularmente aquellas enviadas desde el año de 1852 en el que el Senado expide la orden de destierro en su contra, hasta poco después de su muerte ocurrida en diciembre de 1853. Incluye: cartas enviadas por Federico Arboleda; monseñor Lorenzo Barilli; José A. Barros; Sebastián Bascioni; J.C. Beales, de la Compañía de Jesús; Cabildo de Popayán; Carlos Calvo; José Antonio [Chávez], obispo de Calidonia; Pablo de Blas, de la Compañía de Jesús; Narciso De Francisco Martin; Francisco obispo de La Habana Fleix y Solans; fray José Andrés Forero; Mariano, obispo de Guayana [Fortique]; Domingo Belisario Gómez; Víctor Gómez; Primitivo Grueso; Gregorio Gutiérrez Vergara; Ignacio Gutiérrez Vergara; fray Pedro José Guzmán; Antonio Herrán; Pedro Alcántara Herrán; A.C. Lançon Hermanos; Domingo Lemos; Justo Pastor Lozada; Manuel M. Luna; fray Jacinto Madeleine; Manuel María Mallarino; María de Cristo [priora del Convento]; María Isabela del Carmelo; Juan Antonio Marroquín; Pedro Maciá; fray Pedro Martínez; Valentín Carlos Martínez; Francisco Montoya; José María Obando; Antonino Olano; Juan Francisco Ortiz; José Telésforo Paul; Manuel María Peña; Ana María Pombo; Lino de Pombo; Purificación C. del Sagrado Corazón; José Elías obispo de Caradro [Puyana]; Venancio Restrepo; C. de Rivaud; Gregorio de Jesús Rivera; Ignacio Rivera; Pedro Agustín Rodríguez; Josefa Sáenz de Manzanos; José María Sáiz; Francisco Sauri; Nicolás Savo; Mariano obispo de Tricala [Talavera y Garcés]; Pedro Antonio Torres; Jorge Vargas; fray Eduardo Vásquez; Pedro Vásquez. Fechada en Popayán, Santa Marta, Roma, Nueva York, Quito, Bogotá, Caracas, Cartagena, Medellín, Washington, Brooklyn, Lyon, Kingston, Lima, Cali, México, Cartago, Laval, Pasto, Tierra Negra, Poitiers, El Trapiche, Santa Fé, Villaclara, Bordeaux y Torrice