Standardized Short-Term Acute Heat Stress Assays Resolve Historical Differences in Coral Thermotolerance Across Microhabitat Reef Sites

Coral bleaching is one of the main drivers of reef degradation. Most corals bleach and suffer mortality at just 1–2°C above their maximum monthly mean temperatures, but some species and genotypes resist or recover better than others. Here, we conducted a series of 18‐hr short‐term acute heat stress assays side‐by‐side with a 21‐day long‐term heat stress experiment to assess the ability of both approaches to resolve coral thermotolerance differences reflective of in situ reef temperature thresholds. Using a suite of physiological parameters (photosynthetic efficiency, coral whitening, chlorophyll a , host protein, algal symbiont counts, and algal type association), we assessed bleaching susceptibility of Stylophora pistillata colonies from the windward/exposed and leeward/protected sites of a nearshore coral reef in the central Red Sea, which had previously shown differential mortality during a natural bleaching event. Photosynthetic efficiency was most indicative of the expected higher thermal tolerance in corals from the protected reef site, denoted by an increased retention of dark‐adapted maximum quantum yields at higher temperatures. These differences were resolved using both experimental setups, as corroborated by a positive linear relationship, not observed for the other parameters. Notably, short‐term acute heat stress assays resolved per‐colony (genotype) differences that may have been masked by acclimation effects in the long‐term experiment. Using our newly developed portable experimental system termed the Coral Bleaching Automated Stress System (CBASS), we thus highlight the potential of mobile, standardized short‐term acute heat stress assays to resolve fine‐scale differences in coral thermotolerance. Accordingly, such a system may be suitable for large‐scale determination and complement existing approaches to identify resilient genotypes/reefs for downstream experimental examination and prioritization of reef sites for conservation/restoration. Development of such a framework is consistent with the recommendations of the National Academy of Sciences and the Reef Restoration and Adaptation Program committees for new intervention and restoration strategies

Diagnóstico coproparasitológico de fascioliasis en ovinos y caprinos de Boavita, Boyacá (Colombia)

The aim of this study was to establish the prevalence of Fasciola hepatica through coprological analysis and to identify risk factors associated with the presentation of the parasite in sheep and goats in the municipality of Boavita, Boyacá. The study was observational, descriptive, cross-sectional with simple random sampling. Faecal samples were taken from 297 sheep and 337 goats to identify parasite eggs. The general prevalence was 8.0% (51/634), being 9.1% for sheep and 7.1% for goats. The prevalence in sheep was higher in males (14.8%) than in females (8.5%), likewise, sheep less than one year old (9.3%) and Criolla (11.1%) presented the highest prevalence. In goats, the prevalence was higher in females (7.3%) than in males (5.6%), and those less than one year old (10.5%) and the Alpine breed (8%) presented the greater prevalence. No significant statistical association was found between females and males. The Creole breed was established as a risk factor for sheep.El objetivo del estudio fue establecer la prevalencia de Fasciola hepatica mediante análisis coprológico e identificar factores de riesgo asociados a la presentación del parásito en ovinos y caprinos del municipio de Boavita, Boyacá. El estudio fue observacional, descriptivo de corte transversal con muestreo aleatorio simple. Se tomaron muestras de materia fecal a 297 ovinos y 337 caprinos para identificar los huevos del parásito. La prevalencia general fue de 8.0% (51/634), siendo de 9.1% para ovejas y de 7.1% para cabras. La prevalencia en ovinos fue mayor en machos (14.8%) que en hembras (8.5%); asimismo, los ovinos menores a un año (9.3%) y de Criolla (11.1%) presentaron las prevalencias más altas. En las cabras, la prevalencia fue mayor en hembras (7.3%) que en machos (5.6%), y los menores a un año (10.5%) y de la raza Alpina (8%) presentaron las prevalencias más altas. No se encontró asociación estadística significativa entre hembras y machos. La raza Criolla se estableció como factor de riesgo para los ovinos

MASSIV: Mass Assembly Survey with SINFONI in VVDS. V. The major merger rate of star-forming galaxies at 0.9 < z < 1.8 from IFS-based close pairs

We aim to measure the major merger rate of star-forming galaxies at 0.9 < z <1.8, using close pairs identified from integral field spectroscopy (IFS). We use the velocity field maps obtained with SINFONI/VLT on the MASSIV sample, selected from the star-forming population in the VVDS. We identify physical pairs of galaxies from the measurement of the relative velocity and the projected separation (r_p) of the galaxies in the pair. Using the well constrained selection function of the MASSIV sample we derive the gas-rich major merger fraction (luminosity ratio mu = L_2/L_1 >= 1/4), and, using merger time scales from cosmological simulations, the gas-rich major merger rate at a mean redshift up to z = 1.54. We find a high gas-rich major merger fraction of 20.8+15.2-6.8 %, 20.1+8.0-5.1 % and 22.0+13.7-7.3 % for close pairs with r_p <= 20h^-1 kpc in redshift ranges z = [0.94, 1.06], [1.2, 1.5) and [1.5, 1.8), respectively. This translates into a gas-rich major merger rate of 0.116+0.084-0.038 Gyr^-1, 0.147+0.058-0.037 Gyr^-1 and 0.127+0.079-0.042 Gyr^-1 at z = 1.03, 1.32 and 1.54, respectively. Combining our results with previous studies at z < 1, the gas-rich major merger rate evolves as (1+z)^n, with n = 3.95 +- 0.12, up to z = 1.5. From these results we infer that ~35% of the star-forming galaxies with stellar masses M = 10^10 - 10^10.5 M_Sun have undergone a major merger since z ~ 1.5. We develop a simple model which shows that, assuming that all gas-rich major mergers lead to early-type galaxies, the combined effect of gas-rich and dry mergers is able to explain most of the evolution in the number density of massive early-type galaxies since z ~ 1.5, with our measured gas-rich merger rate accounting for about two-thirds of this evolution.Comment: Published in Astronomy and Astrophysics, 24 pages, 30 figures, 2 tables. Appendix with the residual images from GALFIT added. Minor changes with respect to the initial versio

The host galaxies of luminous type 2 AGNs at z ∼ 0.3–0.4

ABSTRACT: We study the morphological and structural properties of the host galaxies associated with 57 optically selected luminous type 2 active galactic nuclei (AGNs) at z ∼ 0.3–0.4: 16 high-luminosity Seyfert 2 [HLSy2, 8.0 ≤ log(L[oIII]/Lʘ) < 8.3] and 41 obscured [QSO2, log(L[O III]/Lʘ) ≥ 8.3] quasars. With this work, the total number of QSO2s at z < 1 with parametrized galaxies increases from ∼35 to 76. Our analysis is based on Hubble Space Telescope WFPC2 and ACS images that we fit with GALFIT. HLSy2s and QSO2s show a wide diversity of galaxy hosts. The main difference lies in the higher incidence of highly disturbed systems among QSO2s. This is consistent with a scenario in which galaxy interactions are the dominant mechanism triggering nuclear activity at the highest AGN power. There is a strong dependence of galaxy properties with AGN power (assuming L[O III] is an adequate proxy). The relative contribution of the spheroidal component to the total galaxy light (B/T) increases with L[O III]. While systems dominated by the spheroidal component spread across the total range of L[O III], most disc-dominated galaxies concentrate at log(L[O III]/Lʘ)JUM and MVM acknowledge support from the Spanish former Ministerio de Economía y Competitividad through the grants AYA2012-32295 and AYA2015-64346-C2-2-P. FB also acknowledges support from grant AYA2016-77237-C3-1-P from the Spanish Ministry of Economy and Competitiveness (MINECO). JPL acknowledges support from the Spanish Ministerio de Economía y Competitividad through the grant AYA2017-85170-R. BRP acknowledges financial support from the Spanish Ministry of Economy and Competitiveness through grant ESP2015-68964. F.J.C. acknowledges financial support through grant AYA2015-64346-C2-1-P (MINECO/FEDER)

Spectro-photometric close pairs in GOODS-S: major and minor companions of intermediate-mass galaxies

(Abriged) Our goal here is to provide merger frequencies that encompass both major and minor mergers, derived from close pair statistics. We use B-band luminosity- and mass-limited samples from an Spitzer/IRAC-selected catalogue of GOODS-S. We present a new methodology for computing the number of close companions, Nc, when spectroscopic redshift information is partial. We select as close companions those galaxies separated by 6h^-1 kpc < rp < 21h^-1 kpc in the sky plane and with a difference Delta_v <= 500 km s^-1 in redshift space. We provide Nc for four different B-band-selected samples. Nc increases with luminosity, and its evolution with redshift is faster in more luminous samples. We provide Nc of M_star >= 10^10 M_Sun galaxies, finding that the number including minor companions (mass ratio >= 1/10) is roughly two times the number of major companions alone (mass ratio >= 1/3) in the range 0.2 <= z < 1.1. We compare the major merger rate derived by close pairs with the one computed by morphological criteria, finding that both approaches provide similar merger rates for field galaxies when the progenitor bias is taken into account. Finally, we estimate that the total (major+minor) merger rate is ~1.7 times the major merger rate. Only 30% to 50% of the M_star >= 10^10 M_Sun early-type (E/S0/Sa) galaxies that appear z=1 and z=0 may have undergone a major or a minor merger. Half of the red sequence growth since z=1 is therefore unrelated to mergers.Comment: Accepted in A&A. 14 pages, 6 figures, 8 tables. We have tested the method with a local, volume-limited spectroscopic sample

Consensus Guidelines for Advancing Coral Holobiont Genome and Specimen Voucher Deposition

Coral research is being ushered into the genomic era. To fully capitalize on the potential discoveries from this genomic revolution, the rapidly increasing number of high-quality genomes requires effective pairing with rigorous taxonomic characterizations of specimens and the contextualization of their ecological relevance. However, to date there is no formal framework that genomicists, taxonomists, and coral scientists can collectively use to systematically acquire and link these data. Spurred by the recently announced “Coral symbiosis sensitivity to environmental change hub” under the “Aquatic Symbiosis Genomics Project” - a collaboration between the Wellcome Sanger Institute and the Gordon and Betty Moore Foundation to generate gold-standard genome sequences for coral animal hosts and their associated Symbiodiniaceae microalgae (among the sequencing of many other symbiotic aquatic species) - we outline consensus guidelines to reconcile different types of data. The metaorganism nature of the coral holobiont provides a particular challenge in this context and is a key factor to consider for developing a framework to consolidate genomic, taxonomic, and ecological (meta)data. Ideally, genomic data should be accompanied by taxonomic references, i.e., skeletal vouchers as formal morphological references for corals and strain specimens in the case of microalgal and bacterial symbionts (cultured isolates). However, exhaustive taxonomic characterization of all coral holobiont member species is currently not feasible simply because we do not have a comprehensive understanding of all the organisms that constitute the coral holobiont. Nevertheless, guidelines on minimal, recommended, and ideal-case descriptions for the major coral holobiont constituents (coral animal, Symbiodiniaceae microalgae, and prokaryotes) will undoubtedly help in future referencing and will facilitate comparative studies. We hope that the guidelines outlined here, which we will adhere to as part of the Aquatic Symbiosis Genomics Project sub-hub focused on coral symbioses, will be useful to a broader community and their implementation will facilitate cross- and meta-data comparisons and analyses.CV acknowledges funding from the German Research Foundation (DFG), grants 433042944 and 458901010. Open Access publication fees are covered by an institutional agreement of the University of Konstanz

Double blind, randomized controlled trial, to evaluate the effectiveness of a controlled nitric oxide releasing patch versus meglumine antimoniate in the treatment of cutaneous leishmaniasis [NCT00317629]

BACKGROUND: Cutaneous Leishmaniasis is a worldwide disease, endemic in 88 countries, that has shown an increasing incidence over the last two decades. So far, pentavalent antimony compounds have been considered the treatment of choice, with a percentage of cure of about 85%. However, the high efficacy of these drugs is counteracted by their many disadvantages and adverse events. Previous studies have shown nitric oxide to be a potential alternative treatment when administered topically with no serious adverse events. However, due to the unstable nitric oxide release, the topical donors needed to be applied frequently, making the adherence to the treatment difficult. The electrospinning technique has allowed the production of a multilayer transdermal patch that produces a continuous and stable nitric oxide release. The main objective of this study is to evaluate this novel nitric oxide topical donor for the treatment of cutaneous leishmaniasis. METHODS AND DESIGN: A double-blind, randomized, double-masked, placebo-controlled clinical trial, including 620 patients from endemic areas for Leishmaniasis in Colombia was designed to investigate whether this patch is as effective as meglumine antimoniate for the treatment of cutaneous leishmaniasis but with less adverse events. Subjects with ulcers characteristic of cutaneous leishmaniasis will be medically evaluated and laboratory tests and parasitological confirmation performed. After checking the inclusion/exclusion criteria, the patients will be randomly assigned to one of two groups. During 20 days Group 1 will receive simultaneously meglumine antimoniate and placebo of nitric oxide patches while Group 2 will receive placebo of meglumine antimoniate and active nitric oxide patches. During the treatment visits, the medications will be daily administered and the presence of adverse events assessed. During the follow-up, the research group will visit the patients at days 21, 45, 90 and 180. The healing process of the ulcer, the health of the participants, recidivisms and/or reinfection will also be assessed. The evolution of the ulcers will be photographically registered. In case that the effectiveness of the patches is demonstrated, a novel and safe therapeutic alternative for one of the most important public health problems in many countries will be available to patients

UltraVISTA: a new ultra-deep near-infrared survey in COSMOS

In this paper we describe the first data release of the UltraVISTA near-infrared imaging survey of the COSMOS field. We summarise the key goals and design of the survey and provide a detailed description of our data reduction techniques . We provide stacked, sky-subtracted images in $YJHK_{\rm s}$ and narrow-band filters constructed from data collected during the first year of UltraVISTA observations. Our stacked images reach $5\sigma$ $AB$ depths in an aperture of 2\arcsec diameter of $\sim 25$ in $Y$ and $\sim 24$ in $JHK_{\rm s}$ bands and all have sub-arcsecond seeing. To this $5\sigma$ limit, our $K_{\rm s}$ catalogue contains 216,268 sources. We carry out a series of quality assessment tests on our images and catalogues, comparing our stacks with existing catalogues. The $1\sigma$ astrometric RMS in both directions for stars selected with $17.0<K_{\rm s}\rm {(AB)} <19.5$ is \sim 0.08\arcsec in comparison to the publicly-available COSMOS ACS catalogues. Our images are resampled to the same pixel scale and tangent point as the publicly available COSMOS data and so may be easily used to generate multi-colour catalogues using this data. All images and catalogues presented in this paper are publicly available through ESO's "phase 3" archiving and distribution system and from the UltraVISTA web site.Comment: 11 pages, 15 figures, updated to match published version. Error in Fig. 15 in A&A version correcte