PROFESSIONAL DISEASE OF WORKERS OF THE AIRCRAFT PLANT OF ULAN-UDE

In the article the analysis of professional disease of workers of Aircraft plant of Ulan-Ude is presented

Measles (lecture)

The article provides comprehensive review of different aspects of measles. Definition of disease, historical overview, measles in the world epidemiology and in the countries bordering Ukraine over the recent years, particularities of measles epidemiology in Ukraine are given in details. Etiology of measles virus including known genomic structure and viral proteins list, genetic changeability of the virus. Particularities of measles epidemiological process are discussed, criteria of determination of morbidity level and contagiosity of the given disease are outlined. Detailed pathogenesis of measles in different periods of disease is provided, reciprocal influence of the disease and vitamin A metabolism is given. Particularities of humoral and cellular immunological response, including those in early-aged and in patients with immune deficiency are described. Possibility of development of subacute sclerosing panencephalitis is pointed out; currently accepted causes of this complication development, its immunological and virological particularities are summarized. Pathogenetic mecha­nisms of rash development, com­plications as well as morphological changes in different organs and systems are given. The article gives both international and different clinical classifications of measles. Clinical manifestations of typical and atypical measles course are described in details by syndromes and according to disease periods. Particularities of measles course at different premorbid conditions are described. The article provides colored photos which illustrate clinical mani­festations of measles manifestation on the skin and mucosa at different disease periods

Ifluence of seasonity on vitamin D level in patients with HCV infection and healthy people

According to WHO, about 150-200 million people are currently infected with the HCV virus worldwide. Recently, in the professional literature, the number of publications on the role of vitamin D in patients with viral hepatitis C has increased as vitamin D metabolism occurs with the participation of the liver and its deficiency is associated with an increased risk of infectious diseases. The aim of this study was to investigate the effect of seasonal factor on vitamin D (25 hydroxycalciferol) levels in patients with chronic viral hepatitis C and healthy subjects. The study involved 100 patients in the registry of patients with chronic viral hepatitis in the Dnipropetrovsk region. The prevalence and deficiency of vitamin D in patients with chronic viral hepatitis C and conditionally healthy subjects at different times of the year were determined, which showed the presence or absence of a seasonal effect on serum 25(OH)D level. Patients were divided into two groups, depending on the time of the year (autumn-winter and spring-summer), in which the level of 25 (OH) D was determined. The serum was metabolised by vitamin D, which is synthesized by the liver – 25 hydroxycalciferol (25 (OH) D), an indicator of the supply of vitamin D to the human body. Vitamin D levels were evaluated according to the M.F. Holick classification. According to the level of vitamin D patients were divided into 3 groups (patients with normal level, insufficient (suboptimal) level and vitamin D deficiency). The results of the study showed no effect of seasonal factor on the level of 25 (OH) D in the serum of patients with chronic viral hepatitis C. Vitamin D levels are controlled by the time of the year: in spring and summer this indicator is normal, in autumn and winter – seasonal decrease in vitamin D

Корь (лекция)

The article provides comprehensive review of different aspects of measles. Definition of disease, historical overview, measles in the world epidemiology and in the countries bordering Ukraine over the recent years, particularities of measles epidemiology in Ukraine are given in details. Etiology of measles virus including known genomic structure and viral proteins list, genetic changeability of the virus. Particularities of measles epidemiological process are discussed, criteria of determination of morbidity level and contagiosity of the given disease are outlined. Detailed pathogenesis of measles in different periods of disease is provided, reciprocal influence of the disease and vitamin A metabolism is given. Particularities of humoral and cellular immunological response, including those in early-aged and in patients with immune deficiency are described. Possibility of development of subacute sclerosing panencephalitis is pointed out; currently accepted causes of this complication development, its immunological and virological particularities are summarized. Pathogenetic mecha­nisms of rash development, com­plications as well as morphological changes in different organs and systems are given. The article gives both international and different clinical classifications of measles. Clinical manifestations of typical and atypical measles course are described in details by syndromes and according to disease periods. Particularities of measles course at different premorbid conditions are described. The article provides colored photos which illustrate clinical mani­festations of measles manifestation on the skin and mucosa at different disease periods

Neurosyphilis in the practice of clinicians: difficulties of diagnosis

Neurosyphilis presents a complicated diagnostic task for physician’s worldwide, considering its confusing clinical presentations and long-term course, especially its latent and late forms. We present a clinical case of diagnosis of neurosyphilis, meningovascular form, with typical MRI finding and VDRL confirmation. Treatment with penicillin led to clinical improvement of psychopathological changes, whereas complete recovery was not achieved in this case. Neurosyphilis should be included into differential diagnosis of complicated cases of CNS disorder

Comparative analysis of vitamin D contents in patients with chronic viral hepatitis C and healthy.

From scientific literature it is known that vitamin D helps maintain the most important functions of the immune system, there is evidence of its role in inflammatory and metabolic diseases of the liver, including infection with hepatitis C. Aim – to study vitamin D status in patients with chronic viral hepatitis C compared to the healthy ones. We examined 100 patients with chronic viral hepatitis C (main group) and 30 patients without hepatitis C virus (control group). Level of 25 (OH) D in serum was studied by immunochemical method with electrochemiluminescent detection. To verify the diagnosis of vitamin D deficiency and insufficiency, a classification (M.F. Holick, 2011), adopted by the International Institute of Medicine and the Committee of Endocrinologists for clinical practice guidelines, was used. Vitamin D deficiency was found in 44% of people with chronic viral hepatitis C in the main group (mean hydroxycalciferol – 14.36±4.12 ng/ml. Vitamin D deficiency was found in 6,6% in the control group (mean 17,5±8,52 ng/ml). The average vitamin D deficiency in the main group was slightly lower than that in the control group. Factors that affect the metabolism of vitamin D in patients with chronic viral hepatitis C include a lack of vitamin D in blood serum

ЗАБЕЗПЕЧЕНІСТЬ ВІТАМІНОМ D ПАЦІЄНТІВ З ХРОНІЧНИМ ГЕПАТИТОМ С

The aim of the work – to analyze the level of vitamin D (25 hydroxycalciferol (25(OH)D) in patients with chronic hepatitis C (HCV) prior to the start of antiviral therapy. Materials and methods. 100 people who are registered as patients with chronic viral hepatitis in the Dnipropetrovsk region, whose serum was determined by the liver metabolite of vitamin D, 25 hydroxycalciferol (25(OH)D), which is currently indicates the available amount of vitamin D in the human body. Patients were divided according to the level of vitamin D into 3 groups (patients with normal levels, insufficiency and vitamin D deficiency). Evaluation of vitamin D-status was performed according to the classification (M.F. Holick, 2011), according to which the level of 25(OH)D in serum from 75–200 nmol/l or 30–85 ng/ml corresponds to the norm, the level of 25-hydroxycalciferol from 75 nmol/l to 50 nmol/l or 29–20 ng/ml in the blood is considered to be as insufficiency of vitamin D and an indicator lower than 50 nmol/l or less than 20 ng/ml corresponds to a vitamin D deficiency. Results and discussion. The study showed that only 18 (18.0 %) patients had sufficient vitamin D levels, 38 patients (38.0 %) had vitamin D insufficiency, and most patients 44 (44.0 %) were diagnosed with vitamin D deficiency, indicating the apparent relationship between vitamin D levels and chronic hepatitis C. Vitamin D deficiency was found to be prevalent in the age groups of 45–59 years (61.4 %) and 25–44 years (22.7 %). It was determined that vitamin D level among HCV patients did not depend on gender.Мета роботи – вивчити забезпеченість вітаміном D (25 гідроксикальциферол (25(OH)D) хворих на хронічний гепатит С (ХГС) перед початком противірусної терапії (ПВТ). Матеріали і методи. Досліджено 100 осіб, які перебували у реєстрі хворих на хронічний вірусний гепатит у Дніпропетровському регіоні, яким у сироватці крові визначали метаболіт вітаміну D, що синтезується печінкою – 25 гідроксикальциферол (25(OH)D), який на сьогодні є індикатором забезпеченості вітаміном D організму людини. Оцінка вітамін D-статусу проводилась відповідно до класифікації (M.F. Holick, 2011), згідно з якою рівень 25(OH)D у сироватці крові від 75-200 нмоль/л або 30-85 нг/мл є оптимальним, рівень 25-гідроксикальциферолу від 75 нмоль/л до 50 нмоль/л або 29-20 нг/мл у крові – суб­оптимальним рівнем вітаміну D, а показник нижчий 50 нмоль/л або менше 20 нг/мл відповідає дефіциту вітаміну D. Пацієнти були розподілені залежно від рівня вітаміну D на 3 групи (пацієнти з оптимальним рівнем, субоптимальним рівнем і дефіцитом вітаміну D). Результати досліджень та їх обговорення. Результати дослідження продемонстрували, що лише 18 (18,0 %) пацієнтів мали оптимальний рівень вітаміну D, у 38 хворих (38,0 %) виявлено субоптимальний рівень вітаміну D, у більшості хворих – 44 (44,0 %) діагностовано дефіцит вітаміну D, що вказує на певну закономірність між рівнем вітаміну D та ХГС. Було встановлено, що дефіцит вітаміну D переважав у хворих у вікових межах 45-59 років (61,4 %) і 25-44 років (22,7 %). З’ясовано, що вміст вітаміну D при ХГС не залежав від статі й був знижений у більшості хворих жінок і чоловіків

Comparative analysis of vitamin D contents in patients with chronic viral hepatitis C and healthy.

From scientific literature it is known that vitamin D helps maintain the most important functions of the immune system, there is evidence of its role in inflammatory and metabolic diseases of the liver, including infection with hepatitis C. Aim – to study vitamin D status in patients with chronic viral hepatitis C compared to the healthy ones. We examined 100 patients with chronic viral hepatitis C (main group) and 30 patients without hepatitis C virus (control group). Level of 25 (OH) D in serum was studied by immunochemical method with electrochemiluminescent detection. To verify the diagnosis of vitamin D deficiency and insufficiency, a classification (M.F. Holick, 2011), adopted by the International Institute of Medicine and the Committee of Endocrinologists for clinical practice guidelines, was used. Vitamin D deficiency was found in 44% of people with chronic viral hepatitis C in the main group (mean hydroxycalciferol – 14.36±4.12 ng/ml. Vitamin D deficiency was found in 6,6% in the control group (mean 17,5±8,52 ng/ml). The average vitamin D deficiency in the main group was slightly lower than that in the control group. Factors that affect the metabolism of vitamin D in patients with chronic viral hepatitis C include a lack of vitamin D in blood serum

HVint: a strategy for identifying novel protein-protein interactions in Herpes Simplex Virus Type 1

Human herpesviruses are widespread human pathogens with a remarkable impact on worldwide public health. Despite intense decades of research, the molecular details in many aspects of their function remain to be fully characterized. To unravel the details of how these viruses operate, a thorough understanding of the relationships between the involved components is key. Here, we present HVint, a novel protein-protein intra-viral interaction resource for herpes simplex virus type 1 (HSV-1) integrating data from five external sources. To assess each interaction, we used a scoring scheme that takes into consideration aspects such as the type of detection method and the number of lines of evidence. The coverage of the initial interactome was further increased using evolutionary information, by importing interactions reported for other human herpesviruses. These latter interactions constitute, therefore, computational predictions for potential novel interactions in HSV-1. An independent experimental analysis was performed to confirm a subset of our predicted interactions. This subset covers proteins that contribute to nuclear egress and primary envelopment events, including VP26, pUL31, pUL40 and the recently characterized pUL32 and pUL21. Our findings support a coordinated crosstalk between VP26 and proteins such as pUL31, pUS9 and the CSVC complex, contributing to the development of a model describing the nuclear egress and primary envelopment pathways of newly synthesized HSV-1 capsids. The results are also consistent with recent findings on the involvement of pUL32 in capsid maturation and early tegumentation events. Further, they open the door to new hypotheses on virus-specific regulators of pUS9-dependent transport. To make this repository of interactions readily accessible for the scientific community, we also developed a user-friendly and interactive web interface. Our approach demonstrates the power of computational predictions to assist in the design of targeted experiments for the discovery of novel protein-protein interactions