Das Jubiläumsjahr im Überblick

Das Staatsarchiv Hamburg feierte 2010 sein 300-jähriges Bestehen. Sein Vorläufer wurde bereits im Jahre 1293 erstmals erwähnt und somit die Aufbewahrung wichtiger Dokumente der Stadt an zentraler Stelle bezeugt. Doch erst seit dem 11. September 1710, dem Amtsantritt von Nicolaus Stampeel als wissenschaftlicher Archivar bei der Stadt Hamburg, werden Dokumente systematisch ausgewählt, bewahrt und erschlossen. Dieses Jubiläum hat das Staatsarchiv mit einem breit gefächerten Veranstaltungsprogramm begangen, zu dem auch Vorträge und Lesungen gehörten, die in der hier vorliegenden Festschrift abgedruckt sind.In 2010, the Hamburg State Archives celebrated its 300th anniversary. The Archives\u27 forerunner was first mentioned in 1293 and thus testified to the preservation of important documents of the city in a central place. But it was not until September 11th, 1710, when Nicolaus Stampeel took office as a scientific archivist at the City of Hamburg. From then on, documents were systematically selected, preserved and indexed. This anniversary was celebrated by the State Archives with a broad programme of events, which included lectures and readings printed in the commemorative volume available here

Las órdenes militares en la cruzada granadina de Alfonso el Benigno (1329-1334)

A la memoria de la Dra. Regina Sainz de la MazaEn este estudio se recupera la investigación las Órdenes militares catalanoaragonesas durante el siglo XIV. El interés del trabajo radica en el hecho de que en él se considera, de manera conjunta, la actuación en la cruzada de las cuatro Órdenes militares enclavadas en territorio catalanoaragonés, lo cual permite observar tanto su distinto grado de participación como la diferente actitud del monarca hacia cada una de ellas.Peer reviewe

Pseudo Natural Products—Chemical Evolution of Natural Product Structure

Pseudo‐natural products (PNPs) combine natural product (NP) fragments in novel arrangements not accessible by current biosynthesis pathways. As such they can be regarded as non‐biogenic fusions of NP‐derived fragments. They inherit key biological characteristics of the guiding natural product, such as chemical and physiological properties, yet define small molecule chemotypes with unprecedented or unexpected bioactivity. We iterate the design principles underpinning PNP scaffolds and highlight their syntheses and biological investigations. We provide a cheminformatic analysis of PNP collections assessing their molecular properties and shape diversity. We propose and discuss how the iterative analysis of NP structure, design, synthesis, and biological evaluation of PNPs can be regarded as a human‐driven branch of the evolution of natural products, that is, a chemical evolution of natural product structure

Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes

Laccases are useful biocatalysts for many diverse biotechnological applications. In this study we have established efficient and reliable expression systems and high-throughput screenings for the recombinant CotA-laccase from Bacillus subtilis. The expression levels of cotA-laccase were compared in five different Escherichia coli host strains growing in 96-well microtiter plates under different culture conditions. Lower coefficients of variance (around 15%) were achieved using crude cell lysates of BL21 and KRX host strains growing under microaerobic conditions. Reproducible high-throughput screenings for the decolorization of high redox potential azo and anthraquinonic dyes were developed and optimized for identification of variants with increased redox potential. The enzymatic assays developed were tested for the screening of one mutant library from CotA-laccase created by error-prone PCR.(undefined

Experimental and Numerical Investigation of a Multi-Jet Impingement Cooling Configuration

In order to protect turbine blades from thermal damage or thermally induced aging, internal impingement cooling has found common use throughout engine design, both in stationary gas turbines as well as aircraft engines, but also finds applications in other areas requiring cooling. The present investigation is focused on a generic impingement cooling configuration that can be easily modelled with computational fluid dynamics (CFD) and at the same time can be studied in detail experimentally. The acquired experimental data can be directly used for the validation of the CFD simulations, ultimately allowing their application in more complex, realistic configurations where experimental investigations become prohibitively expensive or otherwise impossible. The investigated configuration consists of 9 evenly spaced jets of Reynolds number Re D = 10000 issuing into a square channel that is sealed at one end. The jets directly impinge on a uniformly heated target plate. With previous work on similar configurations well described in literature, the focus of the present contribution is to further exploit the potentials offered by snap-shot based and time-resolved measurements. The flow field within the channel is characterized with both conventional, snap-shot particle image velocimetry (PIV) as well as with high-speed, time-resolved PIV (TR-PIV) to, respectively, capture overview data as well as detailed information on temporally evolving flow structures. In addition, measurements of the unsteady surface temperature distribution on the heated channel wall are performed by means of a newly developed unsteady temperature sensitive paint (iTSP) measurement technique. The interaction of the turbulent jets with the wall and with its neighbors is studied in detail using correlation and spectral analysis as well as modal decomposition. Where possible, this is supplemented with corresponding data obtained from numerical modelling. None of the applied postprocessing methods reveal a significant interaction between jets suggesting that the jet-driven dynamics of heat transfer at the wall are restricted to their immediate vicinity which may simplify the requirements on numerical models of similar cooling configurations

Veröffentlichungen aus dem Staatsarchiv der Freien und Hansestadt Hamburg

Das Staatsarchiv Hamburg feierte 2010 sein 300-jähriges Bestehen. Sein Vorläufer wurde bereits im Jahre 1293 erstmals erwähnt und somit die Aufbewahrung wichtiger Dokumente der Stadt an zentraler Stelle bezeugt. Doch erst seit dem 11. September 1710, dem Amtsantritt von Nicolaus Stampeel als wissenschaftlicher Archivar bei der Stadt Hamburg, werden Dokumente systematisch ausgewählt, bewahrt und erschlossen. Dieses Jubiläum hat das Staatsarchiv mit einem breit gefächerten Veranstaltungsprogramm begangen, zu dem auch Vorträge und Lesungen gehörten, die in der hier vorliegenden Festschrift abgedruckt sind.In 2010, the Hamburg State Archives celebrated its 300th anniversary. The Archives\u27 forerunner was first mentioned in 1293 and thus testified to the preservation of important documents of the city in a central place. But it was not until September 11th, 1710, when Nicolaus Stampeel took office as a scientific archivist at the City of Hamburg. From then on, documents were systematically selected, preserved and indexed. This anniversary was celebrated by the State Archives with a broad programme of events, which included lectures and readings printed in the commemorative volume available here

A Man-Made ATP-Binding Protein Evolved Independent of Nature Causes Abnormal Growth in Bacterial Cells

Recent advances in de novo protein evolution have made it possible to create synthetic proteins from unbiased libraries that fold into stable tertiary structures with predefined functions. However, it is not known whether such proteins will be functional when expressed inside living cells or how a host organism would respond to an encounter with a non-biological protein. Here, we examine the physiology and morphology of Escherichia coli cells engineered to express a synthetic ATP-binding protein evolved entirely from non-biological origins. We show that this man-made protein disrupts the normal energetic balance of the cell by altering the levels of intracellular ATP. This disruption cascades into a series of events that ultimately limit reproductive competency by inhibiting cell division. We now describe a detailed investigation into the synthetic biology of this man-made protein in a living bacterial organism, and the effect that this protein has on normal cell physiology