Identification, characterization, and purification of a 65,000 dalton protein in rat brain that is photolabeled by nitro-containing benzodiazepines

Benzodiazepines bind to two well-characterized classes of nanomolar-affinity binding sites, the central and the peripheral types. Although these sites appear to mediate many of the effects of these compounds, they cannot account for all of the biochemical and physiologic effects of the benzodiazepines. In this investigation, a protein that is photolabeled by NO2-containing benzodiazepines was identified and characterized in rat brain by performing photaffinity labeling experiments with [3H] -clonazepam and [3H] -flunitrazepam. These experiments demonstrate that this photolabeled protein has a molecular weight of 65,000 daltons. Photolabeling of the protein was saturable, inhibited in a stereoselective manner by benzodiazepine enantiomers, inhibited by therapeutically-relevant concentrations of many different NO2-containing benzodiazepines, and was not inhibited by more than 70 non-benzodiazepine compounds. The photolabeled protein is distinct from the central and peripheral sites on the basis of molecular weight, benzodiazepine inhibitory potencies, subcellular localization, and tissue distribution.The protein was purified to apparent homogeneity by Affi-Gel Blue and Agarose Green column chromatography. This two-column purification scheme resulted in a greater than 2000-fold enrichment of the protein with a yield of 35%. Biochemical studies on the purified preparation established that the protein exists as a monomer in the purified state, contains a high relative abundance of glycine residues, and possesses amino acid sequence homology with the beta chain of hemoglobin.This newly-characterized protein may mediate some of the effects of the benzodiazepines that are not associated with the central- or peripheral-type sites. It is also possible that the protein is an enzyme that synthesizes or degrades benzodiazepines

Older people maintaining mental health well-being through resilience : an appreciative inquiry study in four countries

Aim. To explore the experience and strategies of mental health well-being through resilience in older people across the four participating countries. Background. While there is increasing evidence of the way older people maintain physical well-being, there has not been the same emphasis when examining the ways in which older people enhance their resilience and so promote mental health well-being. Design. An Appreciative Inquiry approach was used. Method. A convenience sample of 58 people over the age of 65 years from Australia, UK, Germany, and South Africa were interviewed. Data were analysed using thematic analysis. Results. Participants described their experiences of mental health well-being in relation to: social isolation and loneliness; social worth; self-determination; and security. Strategies utilised include promoting resilience by maintaining community connections and relationships, keeping active, and emotional, practical and spiritual coping. Conclusion. The findings highlight the importance of maintaining mental health well-being through resilience. Although there were some variations between countries, these strategies for maintaining well-being transcended culture and nation. Relevance to clinical practice. Listening to older people through research such as the current study will help to determine what help is needed and how healthcare and policy makers can assist

Extension Event Attendance increases Adoption of weed management practices by sports field managers

Data from a national survey of 348 U.S. sports field managers were used to examine the effects of participation in Cooperative Extension events on the adoption of turfgrass weed management practices. Of the respondents, 94% had attended at least one event in the previous 3 yr. Of this 94%, 97% reported adopting at least one practice as a result of knowledge gained at an Extension turfgrass event. Half of the respondents had adopted four or more practices; a third adopted five or more practices. Nonchemical, cultural practices were the most-adopted practices (65% of respondents). Multiple regression analysis was used to examine factors explaining practice adoption and Extension event attendance. Compared to attending one event, attending three events increased total adoption by an average of one practice. Attending four or more events increased total adoption by two practices. Attending four or more events (compared to one event) increased the odds of adopting six individual practices by 3- to 6-fold, depending on the practice. This suggests that practice adoption could be enhanced by encouraging repeat attendance among past Extension event attendees. Manager experience was a statistically significant predictor of the number of Extension events attended but a poor direct predictor of practice adoption. Experience does not appear to increase adoption directly, but indirectly, via its impact on Extension event attendance. In addition to questions about weed management generally, the survey asked questions specifically about annual bluegrass management. Respondents were asked to rank seven sources of information for their helpfulness in managing annual bluegrass. There was no single dominant information source, but Extension was ranked more than any other source as the most helpful (by 22% of the respondents) and was ranked among the top three by 53%, closely behind field representative/local distributor sources at 54%

‘I will Blow your face off’—Virtual and Physical World Anti-Muslim Hate Crime

Anti-Muslim hate crime is usually viewed in the prism of physical attacks; however, it also occurs in a cyber context, and this reality has considerable consequences for victims. In seeking to help improve our understanding of anti-Muslim hate crime, this article draws on the findings from a project that involved qualitative interviews with Muslim men and women who experienced both virtual and physical world anti-Muslim hate, and reported their experiences to the British government-funded service Tell MAMA (Measuring Anti-Muslim Attacks). In doing so, this article sets out the first ever study to examine the nature, determinants and impacts of both virtual and physical world anti-Muslim hate crime upon Muslim men and Muslim women in the United Kingdom (UK). Correspondingly, we found that victims of both virtual and physical world anti-Muslim hate crime are likely to suffer from emotional stress, anxiety and fear of cyber threats materialising in the ‘real world’

Islamophobia and Twitter: A Typology of Online Hate Against Muslims on Social Media

The Woolwich attack in May 2013 has led to a spate of hate crimes committed against Muslim communities in the United Kingdom. These incidents include Muslim women being targeted for wearing the headscarf and mosques being vandalized. While street level Islamophobia remains an important area of investigation, an equally disturbing picture is emerging with the rise in online anti-Muslim abuse. This article argues that online Islamophobia must be given the same level of attention as street level Islamophobia. It examines 500 tweets from 100 different Twitter users to examine how Muslims are being viewed and targeted by perpetrators of online abuse via the Twitter search engine, and offers a typology of offender characteristics

Herbicide-Resistance in Turf Systems: Insights and Options for Managing Complexity

Due to complex interactions between social and ecological systems, herbicide resistance has classic features of a “wicked problem.” Herbicide-resistant (HR) Poa annua poses a risk to sustainably managing U.S. turfgrass systems, but there is scant knowledge to guide its management. Six focus groups were conducted throughout the United States to gain understanding of socio-economic barriers to adopting herbicide-resistance management practices. Professionals from major turfgrass sectors (golf courses, sports fields, lawn care, and seed/sod production) were recruited as focus-group participants. Discussions emphasized challenges of the weed management of turfgrass systems as compared to agronomic crops. This included greater time constraints for managing weeds and more limited chemical control options. Lack of understanding about the proper use of compounds with different modes of action was identified as a threat to sustainable weed management. There were significant regional differences in perceptions of the existence, geographic scope, and social and ecological causes of HR in managing Poa annua. Effective resistance management will require tailoring chemical and non-chemical practices to the specific conditions of different turfgrass sectors and regions. Some participants thought it would be helpful to have multi-year resistance management programs that are both sector- and species-specific

Doing ‘dangerous’ autoethnography on Islamophobic victimization

This article draws on our different experiences of employing autoethnography when researching Islamophobia, using two independent research projects. In particular, we reflect upon our experiences of Islamophobic victimization as a result of being ‘visibly’ Muslim in public spaces in the UK. We discuss our motivation for employing autoethnography and the role of our insider/outsider status in adopting the role of the Muslim ‘other’ in public spaces. Additionally, we consider the nature, extent and impact of Islamophobic victimization upon ourselves, and the coping mechanisms we employed to deal with it. The article concludes by reflecting upon the advantages, ethical challenges and limitations of using autoethnography when researching Islamophobic victimization

Proactive and politically skilled professionals: What is the relationship with affective occupational commitment?

The aim of this study is to extend research on employee affective commitment in three ways: (1) instead of organizational commitment the focus is on occupational commitment; (2) the role of proactive personality on affective occupational commitment is examined; and (3) occupational satisfaction is examined as a mediator and political skills as moderator in the relationship between proactive personality and affective occupational commitment. Two connected studies, one in a hospital located in the private sector and one in a university located in the public sector, are carried out in Pakistan, drawing on a total sample of over 400 employees. The results show that proactive personality is positively related to affective occupational commitment, and that occupational satisfaction partly mediates the relationship between proactive personality and affective occupational commitment. No effect is found for a moderator effect of political skills in the relationship between proactive personality and affective occupational commitment. Political skills however moderate the relationship between proactive personality and affective organizational commitment