Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Reducing water scarcity by improving water productivity in the United States

Nearly one-sixth of U.S. river basins are unable to consistently meet societal water demands while also providing sufficient water for the environment. Water scarcity is expected to intensify and spread as populations increase, new water demands emerge, and climate changes. Improving water productivity by meeting realistic benchmarks for all water users could allow U.S. communities to expand economic activity and improve environmental flows. Here we utilize a spatially detailed database of water productivity to set realistic benchmarks for over 400 industries and products. We assess unrealized water savings achievable by each industry in each river basin within the conterminous U.S. by bringing all water users up to industry- and region-specific water productivity benchmarks. Some of the most water stressed areas throughout the U.S. West and South have the greatest potential for water savings, with around half of these water savings obtained by improving water productivity in the production of corn, cotton, and alfalfa. By incorporating benchmark-meeting water savings within a national hydrological model (WaSSI), we demonstrate that depletion of river flows across Western U.S. regions can be reduced on average by 6.2–23.2%, without reducing economic production. Lastly, we employ an environmentally extended input-output model to identify the U.S. industries and locations that can make the biggest impact by working with their suppliers to reduce water use ‘upstream’ in their supply chain. The agriculture and manufacturing sectors have the largest indirect water footprint due to their reliance on water-intensive inputs but these sectors also show the greatest capacity to reduce water consumption throughout their supply chains

New water accounting reveals why the Colorado River no longer reaches the sea

Persistent overuse of water supplies from the Colorado River during recent decades has substantially depleted large storage reservoirs and triggered mandatory cutbacks in water use. The river holds critical importance to more than 40 million people and more than two million hectares of cropland. Therefore, a full accounting of where the river’s water goes en route to its delta is necessary. Detailed knowledge of how and where the river’s water is used can aid design of strategies and plans for bringing water use into balance with available supplies. Here we apply authoritative primary data sources and modeled crop and riparian/wetland evapotranspiration estimates to compile a water budget based on average consumptive water use during 2000–2019. Overall water consumption includes both direct human uses in the municipal, commercial, industrial, and agricultural sectors, as well as indirect water losses to reservoir evaporation and water consumed through riparian/wetland evapotranspiration. Irrigated agriculture is responsible for 74% of direct human uses and 52% of overall water consumption. Water consumed for agriculture amounts to three times all other direct uses combined. Cattle feed crops including alfalfa and other grass hays account for 46% of all direct water consumption

Water scarcity and fish imperilment driven by beef production

Human consumption of freshwater is now approaching or surpassing the rate at which water sources are being naturally replenished in many regions, creating water shortage risks for people and ecosystems. Here we assess the impact of human water uses and their connection to water scarcity and ecological damage across the United States, identify primary causes of river dewatering and explore ways to ameliorate them. We find irrigation of cattle-feed crops to be the greatest consumer of river water in the western United States, implicating beef and dairy consumption as the leading driver of water shortages and fish imperilment in the region. We assess opportunities for alleviating water scarcity by reducing cattle-feed production, finding that temporary, rotational fallowing of irrigated feed crops can markedly reduce water shortage risks and improve ecological sustainability. Long-term water security and river ecosystem health will ultimately require Americans to consume less beef that depends on irrigated feed crops